Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom

Detalhes bibliográficos
Autor(a) principal: Daniela Nascimento Amora
Data de Publicação: 2005
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13
Resumo: Crotalus durissus collilineatus is a snake usually found in semideciduous forest, the Cerrado region and its bites constitutes an important health problem. The most serious systemic change and primary cause of death is acute renal failure although the mecanisms of the damaging effects are not totally understood. We investigated the biological effects promoted by Crotalus durissus collilineatus crude venom and its fractions crotoxin and phopholipase A2. The toxic effects of C d collilineatus crude venom were evaluated by the histopathological analysis of organs such as heart, kidney, brain, lung and liver. Wistar rats were inoculated intraperitoneally with C d collilineatus crude venom. The liver showed steatosis and microvacuolation.The other organs showed normal morphological aspects. Renal effects were evaluated by the isolated perfused rat kidney method.The crude venom used at the lowest dose (10Âg) increased perfusion pressure (PP), urinary flow (UF), and glomerular filtration rate (GFR), with maximal effect at 120min (PP: control(120) 110.3+/-3.69; venom(120) 126.8+/-10.2; UF: control(120) 0.19+/-0.03; venom(120) 0.23+/-0.06; GFR: control(120) 0.79+/-0.07; venom(120) 1.17+/-0.4). There was no effect on the percent of sodium tubular transport (%TNa+), the percent of potassium tubular transport (%TK+) and the percent of chloride tubular transport (%TCl-) The highest dose of the crude venom (30Âg) caused a significantly decrease in PP (control(120) 110.3Â3.69; venom(120) 96.7Â8.1), RVR (control(120) 6.42Â0.78; venom(120)4.8Â0.56), UF (control(120) 0.19Â0.03; venom(120) 0.12Â0.01) and GFR (control(120) 0.79Â0.07; venom(120)0.53Â0.09). There was not effect on the percent of electrolytes tubular transport (%TNa+, %TK+ and %TCl-). Crotoxin (10mcg) produced a decrease in GFR (control(120) 0.79Â0.07; crotoxin(120) 0.31Â0.10), while PP, RVR and UF remained stable. Crotoxin also reduced %TK+ (control(120) 69.94Â6.49; crotoxin(120) 33.28Â4.78) and %TCl- (control(120) 79.53Â2.67; crotoxin (120) 64.62Â6.93) with maximal effect at 120min. Kidney perfused with phospholipase A2 (PLA2) showed a decrease in GFR (control(120) 0.79Â0.07; PLA2 (120) 0.52Â0.07) at 120min, whereas PP, RVR, UF and %TNa+ remained stable. PLA2 also reduced %TK+ (control(120) 69.94Â6.49; PLA2 (120) 56.26Â6.81) and %TCl- (control(60) 82.25Â2.72; PLA2 (60) 75.04Â4.26) at 60min. These results altogether indicate that Crotalus durissus collilineatus venom caused significative alterations in the renal parameters as well as hepatic damage.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisStudy about renal effects of <i>Crotalus durissus collilineatus</i> snake venomEstudo dos efeitos renais do veneno da serpente <i>Crotalus durissus collilineatus</i>2005-01-12Helena Serra Azul Monteiro03277470300http://lattes.cnpq.br/3830155707659519Francisca ClÃa FlorenÃo de Sousa31636020372http://lattes.cnpq.br/1180465052181572Gisela Costa CamarÃo06001734372http://lattes.cnpq.br/881251533662289380790739372http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4739922P3Daniela Nascimento AmoraUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em FarmacologiaUFCBRCrotalus Rim PerfusÃoCrotalus Snake venom Crotoxin Kidney PerfusionFARMACOLOGIA CARDIORENALCrotalus durissus collilineatus is a snake usually found in semideciduous forest, the Cerrado region and its bites constitutes an important health problem. The most serious systemic change and primary cause of death is acute renal failure although the mecanisms of the damaging effects are not totally understood. We investigated the biological effects promoted by Crotalus durissus collilineatus crude venom and its fractions crotoxin and phopholipase A2. The toxic effects of C d collilineatus crude venom were evaluated by the histopathological analysis of organs such as heart, kidney, brain, lung and liver. Wistar rats were inoculated intraperitoneally with C d collilineatus crude venom. The liver showed steatosis and microvacuolation.The other organs showed normal morphological aspects. Renal effects were evaluated by the isolated perfused rat kidney method.The crude venom used at the lowest dose (10Âg) increased perfusion pressure (PP), urinary flow (UF), and glomerular filtration rate (GFR), with maximal effect at 120min (PP: control(120) 110.3+/-3.69; venom(120) 126.8+/-10.2; UF: control(120) 0.19+/-0.03; venom(120) 0.23+/-0.06; GFR: control(120) 0.79+/-0.07; venom(120) 1.17+/-0.4). There was no effect on the percent of sodium tubular transport (%TNa+), the percent of potassium tubular transport (%TK+) and the percent of chloride tubular transport (%TCl-) The highest dose of the crude venom (30Âg) caused a significantly decrease in PP (control(120) 110.3Â3.69; venom(120) 96.7Â8.1), RVR (control(120) 6.42Â0.78; venom(120)4.8Â0.56), UF (control(120) 0.19Â0.03; venom(120) 0.12Â0.01) and GFR (control(120) 0.79Â0.07; venom(120)0.53Â0.09). There was not effect on the percent of electrolytes tubular transport (%TNa+, %TK+ and %TCl-). Crotoxin (10mcg) produced a decrease in GFR (control(120) 0.79Â0.07; crotoxin(120) 0.31Â0.10), while PP, RVR and UF remained stable. Crotoxin also reduced %TK+ (control(120) 69.94Â6.49; crotoxin(120) 33.28Â4.78) and %TCl- (control(120) 79.53Â2.67; crotoxin (120) 64.62Â6.93) with maximal effect at 120min. Kidney perfused with phospholipase A2 (PLA2) showed a decrease in GFR (control(120) 0.79Â0.07; PLA2 (120) 0.52Â0.07) at 120min, whereas PP, RVR, UF and %TNa+ remained stable. PLA2 also reduced %TK+ (control(120) 69.94Â6.49; PLA2 (120) 56.26Â6.81) and %TCl- (control(60) 82.25Â2.72; PLA2 (60) 75.04Â4.26) at 60min. These results altogether indicate that Crotalus durissus collilineatus venom caused significative alterations in the renal parameters as well as hepatic damage.A serpente Crotalus durissus collilineatus à encontrada na regiÃo do Cerrado e o acidente causado por esta espÃcie constitui um problema de saÃde pÃblica. A insuficiÃncia renal aguda à a alteraÃÃo sistÃmica mais sÃria, apesar dos seus mecanismos nÃo serem completamente esclarecidos. O objetivo deste trabalho foi estudar os efeitos biolÃgicos produzidos pelo veneno bruto de Crotalus durissus collilineatus e pelas fraÃÃes crotoxina e fosfolipase A2. Foi realizado o estudo da toxicidade aguda do veneno bruto de C d collilineatus, atravÃs da anÃlise histolÃgica de coraÃÃo, cÃrebro, rins, pulmÃes e fÃgado de ratos tratados com doses crescente do veneno. Nesse estudo foi observada a presenÃa de alteraÃÃes hepÃticas como microvacualizaÃÃo e esteatose, enquanto os demais ÃrgÃos apresentaram aspectos normais. Os efeitos renais foram avaliados atravÃs do mÃtodo de perfusÃo de rim isolado de rato. O veneno bruto, usado na dose de 10Âg, causou um aumento na pressÃo de perfusÃo (PP), no fluxo urinÃrio (FU) e no ritmo de filtraÃÃo glomerular (RFG), com efeito mÃximo aos 120min (PP: controle(120) 110,3+/-3,69; veneno(120) 126,8+/-10,2;FU: controle(120) 0,19+/-0,03; veneno(120) 0,23+/-0,06; RFG: controle(120) 0,79+/-0,07; veneno(120) 1,17+/-0,4). Essa dose nÃo produziu alteraÃÃes no transporte tubular de sÃdio (%TNa+), potÃssio (%TK+) e de cloreto (%TCl-). A dose mais elevada de veneno bruto (30Âg) causou um decrÃscimo significativo, com efeito mÃximo, aos 120min, na PP(controle(120) 110,3+/-3,69; veneno(120) 96,7+/-8,1), na RVR (controle(120) 6,42+/-0,78; veneno(120) 4,8+/-0,56), no FU (controle(120) 0,19+/-0,03; veneno(120) 0,12+/-0,01) e no RFG (controle(120) 0,79+/-0,07; veneno(120) 0,53+/-0,09). NÃo ocorreram alteraÃÃes no transporte tubular de eletrÃlitos. A fraÃÃo crotoxina nÃo produziu alteraÃÃes no RFG (controle(120) 0,79+/-0,07; crotoxina(120) 0,31+/-0,10). Esta fraÃÃo tambÃm reduziu os %TK+ (controle(120) 69,94+/-6,49; crotoxina(120) 33,28+/-4,78) e %TCl- (controle(120) 79,53+/-2,67, crotoxina(120) 64,62+/-6,93). O grupo perfundido com FLA2 apresentou um decrÃscimo no RFG (controle(120) 0,79+/-0,07; FLA2 (120) 0,52+/-0,07), enquanto a PP, a RVR, o FU e o %TNa+ permaneceram estÃveis. Foram observadas reduÃÃes no %TK+ aos 120min (controle(120) 69,94+/-6,49; FLA2(120) 56,26+/-6,81) e aos 60min, no %TCl- (controle(60) 82,25+/-2,72; FLA2(60) 75,04+/-4,26). Foi observada degeneraÃÃo hidrÃpico vacuolar e a presenÃa de material proteinÃceo nos tÃbulos renais, na anÃlise histolÃgica dos rins perfundidos tanto com veneno bruto quanto com as fraÃÃes. Estes resultados indicam que o veneno de Crotalus durissus collilineatus causou alteraÃÃes significativas nos parÃmetros renais e nos aspectos morfolÃgicos hepÃticos.FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicoCoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:13:06Zmail@mail.com -
dc.title.en.fl_str_mv Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
dc.title.alternative.pt.fl_str_mv Estudo dos efeitos renais do veneno da serpente <i>Crotalus durissus collilineatus</i>
title Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
spellingShingle Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
Daniela Nascimento Amora
Crotalus
Rim
PerfusÃo
Crotalus
Snake venom
Crotoxin
Kidney
Perfusion
FARMACOLOGIA CARDIORENAL
title_short Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
title_full Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
title_fullStr Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
title_full_unstemmed Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
title_sort Study about renal effects of <i>Crotalus durissus collilineatus</i> snake venom
author Daniela Nascimento Amora
author_facet Daniela Nascimento Amora
author_role author
dc.contributor.advisor1.fl_str_mv Helena Serra Azul Monteiro
dc.contributor.advisor1ID.fl_str_mv 03277470300
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3830155707659519
dc.contributor.referee1.fl_str_mv Francisca ClÃa FlorenÃo de Sousa
dc.contributor.referee1ID.fl_str_mv 31636020372
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1180465052181572
dc.contributor.referee2.fl_str_mv Gisela Costa CamarÃo
dc.contributor.referee2ID.fl_str_mv 06001734372
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8812515336622893
dc.contributor.authorID.fl_str_mv 80790739372
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4739922P3
dc.contributor.author.fl_str_mv Daniela Nascimento Amora
contributor_str_mv Helena Serra Azul Monteiro
Francisca ClÃa FlorenÃo de Sousa
Gisela Costa CamarÃo
dc.subject.por.fl_str_mv Crotalus
Rim
PerfusÃo
topic Crotalus
Rim
PerfusÃo
Crotalus
Snake venom
Crotoxin
Kidney
Perfusion
FARMACOLOGIA CARDIORENAL
dc.subject.eng.fl_str_mv Crotalus
Snake venom
Crotoxin
Kidney
Perfusion
dc.subject.cnpq.fl_str_mv FARMACOLOGIA CARDIORENAL
dc.description.sponsorship.fl_txt_mv FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
dc.description.abstract.por.fl_txt_mv Crotalus durissus collilineatus is a snake usually found in semideciduous forest, the Cerrado region and its bites constitutes an important health problem. The most serious systemic change and primary cause of death is acute renal failure although the mecanisms of the damaging effects are not totally understood. We investigated the biological effects promoted by Crotalus durissus collilineatus crude venom and its fractions crotoxin and phopholipase A2. The toxic effects of C d collilineatus crude venom were evaluated by the histopathological analysis of organs such as heart, kidney, brain, lung and liver. Wistar rats were inoculated intraperitoneally with C d collilineatus crude venom. The liver showed steatosis and microvacuolation.The other organs showed normal morphological aspects. Renal effects were evaluated by the isolated perfused rat kidney method.The crude venom used at the lowest dose (10Âg) increased perfusion pressure (PP), urinary flow (UF), and glomerular filtration rate (GFR), with maximal effect at 120min (PP: control(120) 110.3+/-3.69; venom(120) 126.8+/-10.2; UF: control(120) 0.19+/-0.03; venom(120) 0.23+/-0.06; GFR: control(120) 0.79+/-0.07; venom(120) 1.17+/-0.4). There was no effect on the percent of sodium tubular transport (%TNa+), the percent of potassium tubular transport (%TK+) and the percent of chloride tubular transport (%TCl-) The highest dose of the crude venom (30Âg) caused a significantly decrease in PP (control(120) 110.3Â3.69; venom(120) 96.7Â8.1), RVR (control(120) 6.42Â0.78; venom(120)4.8Â0.56), UF (control(120) 0.19Â0.03; venom(120) 0.12Â0.01) and GFR (control(120) 0.79Â0.07; venom(120)0.53Â0.09). There was not effect on the percent of electrolytes tubular transport (%TNa+, %TK+ and %TCl-). Crotoxin (10mcg) produced a decrease in GFR (control(120) 0.79Â0.07; crotoxin(120) 0.31Â0.10), while PP, RVR and UF remained stable. Crotoxin also reduced %TK+ (control(120) 69.94Â6.49; crotoxin(120) 33.28Â4.78) and %TCl- (control(120) 79.53Â2.67; crotoxin (120) 64.62Â6.93) with maximal effect at 120min. Kidney perfused with phospholipase A2 (PLA2) showed a decrease in GFR (control(120) 0.79Â0.07; PLA2 (120) 0.52Â0.07) at 120min, whereas PP, RVR, UF and %TNa+ remained stable. PLA2 also reduced %TK+ (control(120) 69.94Â6.49; PLA2 (120) 56.26Â6.81) and %TCl- (control(60) 82.25Â2.72; PLA2 (60) 75.04Â4.26) at 60min. These results altogether indicate that Crotalus durissus collilineatus venom caused significative alterations in the renal parameters as well as hepatic damage.
A serpente Crotalus durissus collilineatus à encontrada na regiÃo do Cerrado e o acidente causado por esta espÃcie constitui um problema de saÃde pÃblica. A insuficiÃncia renal aguda à a alteraÃÃo sistÃmica mais sÃria, apesar dos seus mecanismos nÃo serem completamente esclarecidos. O objetivo deste trabalho foi estudar os efeitos biolÃgicos produzidos pelo veneno bruto de Crotalus durissus collilineatus e pelas fraÃÃes crotoxina e fosfolipase A2. Foi realizado o estudo da toxicidade aguda do veneno bruto de C d collilineatus, atravÃs da anÃlise histolÃgica de coraÃÃo, cÃrebro, rins, pulmÃes e fÃgado de ratos tratados com doses crescente do veneno. Nesse estudo foi observada a presenÃa de alteraÃÃes hepÃticas como microvacualizaÃÃo e esteatose, enquanto os demais ÃrgÃos apresentaram aspectos normais. Os efeitos renais foram avaliados atravÃs do mÃtodo de perfusÃo de rim isolado de rato. O veneno bruto, usado na dose de 10Âg, causou um aumento na pressÃo de perfusÃo (PP), no fluxo urinÃrio (FU) e no ritmo de filtraÃÃo glomerular (RFG), com efeito mÃximo aos 120min (PP: controle(120) 110,3+/-3,69; veneno(120) 126,8+/-10,2;FU: controle(120) 0,19+/-0,03; veneno(120) 0,23+/-0,06; RFG: controle(120) 0,79+/-0,07; veneno(120) 1,17+/-0,4). Essa dose nÃo produziu alteraÃÃes no transporte tubular de sÃdio (%TNa+), potÃssio (%TK+) e de cloreto (%TCl-). A dose mais elevada de veneno bruto (30Âg) causou um decrÃscimo significativo, com efeito mÃximo, aos 120min, na PP(controle(120) 110,3+/-3,69; veneno(120) 96,7+/-8,1), na RVR (controle(120) 6,42+/-0,78; veneno(120) 4,8+/-0,56), no FU (controle(120) 0,19+/-0,03; veneno(120) 0,12+/-0,01) e no RFG (controle(120) 0,79+/-0,07; veneno(120) 0,53+/-0,09). NÃo ocorreram alteraÃÃes no transporte tubular de eletrÃlitos. A fraÃÃo crotoxina nÃo produziu alteraÃÃes no RFG (controle(120) 0,79+/-0,07; crotoxina(120) 0,31+/-0,10). Esta fraÃÃo tambÃm reduziu os %TK+ (controle(120) 69,94+/-6,49; crotoxina(120) 33,28+/-4,78) e %TCl- (controle(120) 79,53+/-2,67, crotoxina(120) 64,62+/-6,93). O grupo perfundido com FLA2 apresentou um decrÃscimo no RFG (controle(120) 0,79+/-0,07; FLA2 (120) 0,52+/-0,07), enquanto a PP, a RVR, o FU e o %TNa+ permaneceram estÃveis. Foram observadas reduÃÃes no %TK+ aos 120min (controle(120) 69,94+/-6,49; FLA2(120) 56,26+/-6,81) e aos 60min, no %TCl- (controle(60) 82,25+/-2,72; FLA2(60) 75,04+/-4,26). Foi observada degeneraÃÃo hidrÃpico vacuolar e a presenÃa de material proteinÃceo nos tÃbulos renais, na anÃlise histolÃgica dos rins perfundidos tanto com veneno bruto quanto com as fraÃÃes. Estes resultados indicam que o veneno de Crotalus durissus collilineatus causou alteraÃÃes significativas nos parÃmetros renais e nos aspectos morfolÃgicos hepÃticos.
description Crotalus durissus collilineatus is a snake usually found in semideciduous forest, the Cerrado region and its bites constitutes an important health problem. The most serious systemic change and primary cause of death is acute renal failure although the mecanisms of the damaging effects are not totally understood. We investigated the biological effects promoted by Crotalus durissus collilineatus crude venom and its fractions crotoxin and phopholipase A2. The toxic effects of C d collilineatus crude venom were evaluated by the histopathological analysis of organs such as heart, kidney, brain, lung and liver. Wistar rats were inoculated intraperitoneally with C d collilineatus crude venom. The liver showed steatosis and microvacuolation.The other organs showed normal morphological aspects. Renal effects were evaluated by the isolated perfused rat kidney method.The crude venom used at the lowest dose (10Âg) increased perfusion pressure (PP), urinary flow (UF), and glomerular filtration rate (GFR), with maximal effect at 120min (PP: control(120) 110.3+/-3.69; venom(120) 126.8+/-10.2; UF: control(120) 0.19+/-0.03; venom(120) 0.23+/-0.06; GFR: control(120) 0.79+/-0.07; venom(120) 1.17+/-0.4). There was no effect on the percent of sodium tubular transport (%TNa+), the percent of potassium tubular transport (%TK+) and the percent of chloride tubular transport (%TCl-) The highest dose of the crude venom (30Âg) caused a significantly decrease in PP (control(120) 110.3Â3.69; venom(120) 96.7Â8.1), RVR (control(120) 6.42Â0.78; venom(120)4.8Â0.56), UF (control(120) 0.19Â0.03; venom(120) 0.12Â0.01) and GFR (control(120) 0.79Â0.07; venom(120)0.53Â0.09). There was not effect on the percent of electrolytes tubular transport (%TNa+, %TK+ and %TCl-). Crotoxin (10mcg) produced a decrease in GFR (control(120) 0.79Â0.07; crotoxin(120) 0.31Â0.10), while PP, RVR and UF remained stable. Crotoxin also reduced %TK+ (control(120) 69.94Â6.49; crotoxin(120) 33.28Â4.78) and %TCl- (control(120) 79.53Â2.67; crotoxin (120) 64.62Â6.93) with maximal effect at 120min. Kidney perfused with phospholipase A2 (PLA2) showed a decrease in GFR (control(120) 0.79Â0.07; PLA2 (120) 0.52Â0.07) at 120min, whereas PP, RVR, UF and %TNa+ remained stable. PLA2 also reduced %TK+ (control(120) 69.94Â6.49; PLA2 (120) 56.26Â6.81) and %TCl- (control(60) 82.25Â2.72; PLA2 (60) 75.04Â4.26) at 60min. These results altogether indicate that Crotalus durissus collilineatus venom caused significative alterations in the renal parameters as well as hepatic damage.
publishDate 2005
dc.date.issued.fl_str_mv 2005-01-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Farmacologia
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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