Clinical, laboratory and microbiological evaluation of treatment with antibiotic pulps of permanent teeth with incomplete rhizogenesis necrosed by trauma

• Autor(a) principal: Adriana Kelly de Sousa Santiago Barbosa
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
• Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=18244
• topic: ODONTOPEDIATRIA

The aim of this study was to evaluate the effects of an antimicrobial paste on the treatment of necrosed to trauma permanent teeth and to verify the presence of microorganisms in the root canal as well as to identify them by performing the polymerase chain reaction (PCR). Thus, two studies were performed: a clinical and microbiological investigation and another one in vitro. Patients who presented open apex necrosed anterior permanent teeth due to trauma and incomplete rhizogenesis were eligible to participate. In the in vitro study, 60 single-rooted specimens were used and their length and root apical diameter were standardized, being contaminated with Enterococcus faecalis for 30 days. In the clinical trial, patients underwent the clinical procedures of the revascularization technique that uses sodium hypochlorite and double antibiotic paste to disinfect the root canal and aggregated trioxide mineral (MTA) as biocompatible material to seal the root canal cervical third. The purpose was to investigate the efficacy of the double antimicrobial paste action through microbiological assessment by bacterial growth analysis and PCR technique in samples collected after endodontic access (C1), after sodium hypochlorite (2.5%) irrigation (C2) and at the end of a 30-day period of use of the double antimicrobial paste in the root canal (C3). The clinical success was assessed by observing the rhizogenesis, dentinary wall thickening, and apex closing through periapical radiographs and localized tomography. Also, the absence of signal and symptoms was evaluated, as well as the absence of periapical lesions. 14 patients were treated, 7 male subjects (50%) and 7 female subjects (50%) whose age ranged from 6-14 years old. Most part of the teeth was affected by lateral luxation (73.3%) and the other (26,3%) cases of dental trauma were avulsion, intrusion, extrusion, and subluxation associated or not with crown fracture without pulp exposition. The percentage of the success reaching 93%. Also, we can observe that the antimicrobial pastes, among them the triple and double ones, presented excellent inhibitory effects on E. faecalis. The two pastes were more efficient in reducing the E. faecalis growth than the calcium hydroxide paste. Hence, the double antimicrobial paste can be considered an antimicrobial agent efficient and comparable to the triple antimicrobial and calcium hydroxide pastes.