Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis

Detalhes bibliográficos
Autor(a) principal: Adriana Kelly de Sousa Santiago
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9471
Resumo: There is an agreement that in dental treatments with incomplete rizogenese it is necessary an intracanal medication with high antibacterial effect because the use of files and standard protocol based on instrumentation must be avoided to protect the fragile dental element from extra hazard. Several endodontic dressings have been used with this purpose, alone or associated to a copious irrigation procedure. However, it is not yet standardized in the literature a unique medication with protracted and excellent antimicrobial effect, associated to a feasible handling and insertion into the duct. The purpose of this study was to evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis by means of agar disk diffusion method. Accordingly, it was created a polypropylene device to simulate the release characteristics of the main root canal. The efficacy tests were performed over a period of 30 days. The formulations studied were distributed in triplicate just as follows: Triantibiotic Paste (metronidazole, ciprofloxacin and minocycline); Biantibiotic Paste (metronidazole and ciprofloxacin); Ciprofloxacin Paste; Amoxilin Paste; Calcium hydroxide paste; 0.9% saline (control group). All pastes were still allotted into 2 categories: open and closed apex. The devices remained in the medium at 37ÂC over 30 days (static). Samples used for the measurement of biologic response were collected in pre-fixed times: H1(1st hour), H6(6th hour), H24(24th hour), D3(3rd day), D7(7th day), D14(14th day) and D30(30th day) in the course of thirty days. At every settled time, 20μL of solution was removed from each device and embedded on sterile paper discs with 6mm of diameter, being the equivalent volume replaced with fresh release medium. After, the discs were transferred with sterile tweezersg to the surface of agar-BHI medium previously inoculated with bacteria over petri plaques. From the devices with closed apex, it was removed 20μL of solution from above the paste surface, avoiding any directly touch on it. This collect aimed to evaluate the antimicrobial effect of different preparations inside the root canal. From the devices with open apex it was removed 20μL of the solution in which the plastic device was submerged, in order to measure the action of the diffused antibiotic to the periapical region through the apex. From the inhibition halos obtained in each case it was possible to rank the formulations in order of effectiveness. The Amoxicillin paste presented an initial antimicrobial effect more robust, keeping it throughout the experiment. However from the second week there was no more significant difference between this and the other antibiotic pastes (triantibiotic and biantibiotic). These pastes had an initial effect less representative than amoxicillin, however the effect became similar from the second week. Nevertheless, calcium hydroxide paste had a discreet effect initially and it was completely ceased already after the 3rd day. Even if Amoxicillin paste presented a superior result among the studied pastes, it was noticed color changes associated to degradation signs after two weeks. The findings show that these antibiotics pastes posed an excellent pharmacological effect, apart from calcium hydroxide. It can be concluded that all the antibiotics pastes, among them the pastes of amoxicillin, triantibiotic and biantibiotic were effective against E. faecalis. As a result its use as dressing for teeth with incomplete risogenesis in may be a good alternative. Among the advantages it is the microbial effectiveness, as well as the feasible manipulation during the clinical procedure. When comparing biantibiotic and triantibiotic pastes, the first showed to be almost equally effective, being a great choice for the clinical treatments of anterior teeth, where the pigmentation caused by minocycline antibiotic could present a drawback in the final result of the treatment.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEvaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalisAvaliaÃÃo in vitro da efetividade de diferentes pastas antibiÃticas utilizadas para curativos endodÃnticos sobre o E. Faecalis2013-03-07Josà Jeovà Siebra Moreira Neto39125432320http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4773258P9Francisco Fabio Oliveira de Sousa92923844330http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4777925P5MÃnica Sampaio do Vale23010134304http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4786660H3ClÃudio Maniglia Ferreira14262563880http://lattes.cnpq.br/1944193921428069 00462817385 http://lattes.cnpq.br/1342838696403276Adriana Kelly de Sousa SantiagoUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em OdontologiaUFCBRPasta antibiÃticaRegeneraÃÃo endodÃnticaApicificaÃÃoPasta antibiotic endodontic regeneration apexification endodontics antibiotics Enterococcus faecalisODONTOPEDIATRIAThere is an agreement that in dental treatments with incomplete rizogenese it is necessary an intracanal medication with high antibacterial effect because the use of files and standard protocol based on instrumentation must be avoided to protect the fragile dental element from extra hazard. Several endodontic dressings have been used with this purpose, alone or associated to a copious irrigation procedure. However, it is not yet standardized in the literature a unique medication with protracted and excellent antimicrobial effect, associated to a feasible handling and insertion into the duct. The purpose of this study was to evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis by means of agar disk diffusion method. Accordingly, it was created a polypropylene device to simulate the release characteristics of the main root canal. The efficacy tests were performed over a period of 30 days. The formulations studied were distributed in triplicate just as follows: Triantibiotic Paste (metronidazole, ciprofloxacin and minocycline); Biantibiotic Paste (metronidazole and ciprofloxacin); Ciprofloxacin Paste; Amoxilin Paste; Calcium hydroxide paste; 0.9% saline (control group). All pastes were still allotted into 2 categories: open and closed apex. The devices remained in the medium at 37ÂC over 30 days (static). Samples used for the measurement of biologic response were collected in pre-fixed times: H1(1st hour), H6(6th hour), H24(24th hour), D3(3rd day), D7(7th day), D14(14th day) and D30(30th day) in the course of thirty days. At every settled time, 20μL of solution was removed from each device and embedded on sterile paper discs with 6mm of diameter, being the equivalent volume replaced with fresh release medium. After, the discs were transferred with sterile tweezersg to the surface of agar-BHI medium previously inoculated with bacteria over petri plaques. From the devices with closed apex, it was removed 20μL of solution from above the paste surface, avoiding any directly touch on it. This collect aimed to evaluate the antimicrobial effect of different preparations inside the root canal. From the devices with open apex it was removed 20μL of the solution in which the plastic device was submerged, in order to measure the action of the diffused antibiotic to the periapical region through the apex. From the inhibition halos obtained in each case it was possible to rank the formulations in order of effectiveness. The Amoxicillin paste presented an initial antimicrobial effect more robust, keeping it throughout the experiment. However from the second week there was no more significant difference between this and the other antibiotic pastes (triantibiotic and biantibiotic). These pastes had an initial effect less representative than amoxicillin, however the effect became similar from the second week. Nevertheless, calcium hydroxide paste had a discreet effect initially and it was completely ceased already after the 3rd day. Even if Amoxicillin paste presented a superior result among the studied pastes, it was noticed color changes associated to degradation signs after two weeks. The findings show that these antibiotics pastes posed an excellent pharmacological effect, apart from calcium hydroxide. It can be concluded that all the antibiotics pastes, among them the pastes of amoxicillin, triantibiotic and biantibiotic were effective against E. faecalis. As a result its use as dressing for teeth with incomplete risogenesis in may be a good alternative. Among the advantages it is the microbial effectiveness, as well as the feasible manipulation during the clinical procedure. When comparing biantibiotic and triantibiotic pastes, the first showed to be almost equally effective, being a great choice for the clinical treatments of anterior teeth, where the pigmentation caused by minocycline antibiotic could present a drawback in the final result of the treatment. Existe um consenso de que no tratamento de dentes com rizogÃnese incompleta à necessÃria uma medicaÃÃo intracanal com mÃximo efeito antibacteriano, pois o uso de limas e o protocolo convencional de instrumentaÃÃo devem ser evitados para que nÃo torne o elemento dental ainda mais frÃgil. Diversos curativos endodÃnticos tÃm sido utilizados com este objetivo, bem como o uso de uma irrigaÃÃo mais copiosa e com irrigante mais efetivo. No entanto, ainda nÃo està padronizada na literatura uma medicaÃÃo com efeito antibiÃtico prolongado e efetivo, alÃm de fÃcil manipulaÃÃo e inserÃÃo no conduto. O objetivo deste trabalho foi avaliar in vitro a eficÃcia de diferentes pastas antimicrobianas utilizadas como medicaÃÃo intracanal sobre o patÃgeno Enterococcus faecalis utilizando o mÃtodo de disco - difusÃo em Ãgar. Para isso, foi confeccionado um dispositivo de polipropileno que possui caracterÃsticas semelhantes ao canal radicular principal. Os testes de eficÃcia foram realizados por um perÃodo de 30 dias. Foram analisadas as seguintes formulaÃÃes: Pasta triantibiÃtica (metronidazol, ciprofloxacino e minociclina); Pasta biantibiÃtica (metronidazol e ciprofloxacino); Pasta de Ciprofloxacino; Pasta de Amoxicilina; Pasta de hidrÃxido de cÃlcio; SoluÃÃo fisiolÃgica 0,9% (grupo controle). Todas as pastas foram ainda alocadas em duas categorias: Ãpice aberto e Ãpice fechado e ensaiadas em triplicata. Os dispositivos permaneceram em estufa a 37ÂC durante 30 dias (sem agitaÃÃo), sendo as coletas para aferiÃÃo da resposta biolÃgica realizadas em perÃodos preestabelecidos: H1(1 hora), H6(6 hora), H24(24 hora), D3(3 dia), D7(7 dia), D14(14 dia) e D30(30 dia) no decorrer de 30 dias. A cada coleta foram retirados de cada dispositivo 20ÂL de soluÃÃo e depositados sobre discos de papel de filtro estÃreis com 6 mm de diÃmetro. Em seguida, os discos de papel embebidos foram transferidos com pinÃa estÃril para a superfÃcie das placas de petri previamente semeadas com a bactÃria. Dos dispositivos com Ãpice fechado foram removidos 20ÂL da soluÃÃo que estava sobre a superfÃcie da pasta, tendo o cuidado para nÃo tocÃ-la. Este procedimento teve o intuito de medir a aÃÃo antimicrobiana direta dos diferentes preparados no interior do conduto radicular. A partir dos dispositivos com Ãpice aberto foram retirados 20ÂL da soluÃÃo na qual o dispositivo plÃstico encontrava-se imerso, com o intuito de medir a aÃÃo do preparado difundido à regiÃo periapical. A partir dos halos de inibiÃÃo obtidos, foi possÃvel observar quais preparados foram mais eficazes com respeito ao seu efeito antibacteriano. A pasta de amoxicilina apresentou o maior efeito antimicrobiano inicial, mantendo-o durante todo o experimento, no entanto, a partir da segunda semana nÃo houve mais diferenÃa estatÃstica entre as pastas antibiÃticas e o efeito tornou-se semelhante. As pastas triantibiÃtica e biantibiÃtica tiveram efeito inicial menor do que o da Amoxicilina, porÃm o efeito foi igualado a partir da segunda semana. Por outro lado, a pasta de hidrÃxido de cÃlcio teve um efeito discreto inicialmente, o qual foi totalmente cessado jà apÃs o 3 dia. Ainda que a pasta de Amoxicilina tenha apresentado os melhores resultados dentre as pastas estudadas, foi evidenciado o aparecimento de cor escurecida associada a sinais de degradaÃÃo apÃs duas semanas. Este estudo mostrou que as pastas antibiÃticas tiveram um excelente efeito farmacolÃgico, ao contrÃrio da pasta de hidrÃxido de cÃlcio. Pode-se concluir que as pastas antibiÃticas, dentre elas as pastas de Amoxicilina, triantibiÃtica e biantibiÃtica apresentaram um excelente efeito sobre o micro-organismo E. faecalis. Portanto, seu uso como medicaÃÃo intracanal nos casos de dentes com rizogÃnese incompleta pode ser uma Ãtima alternativa, estando ainda a facilidade de manipulaÃÃo durante o procedimento clÃnico atrelada à eficÃcia microbicida. Quando comparadas, a pasta biantibiÃtica mostrou-se quase que igualmente efetiva a triantibiÃtica, podendo ser uma Ãtima escolha em casos clÃnicos de tratamento de dentes anteriores, sempre e quando a pigmentaÃÃo causada pelo fÃrmaco Minociclina possa comprometer o resultado final do tratamento. PÃde-se concluir que as pastas antibiÃticas, dentre elas as pastas de amoxicilina, triantibiÃtica e biantibiÃtica apresentaram excelente efeito inibitÃrio sobre o micro-organismo E. faecalis. Portanto, o seu uso como medicaÃÃo intracanal nos casos de dentes com rizogÃnese incompleta pode ser uma excelente alternativa.CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9471application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:22:35Zmail@mail.com -
dc.title.en.fl_str_mv Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
dc.title.alternative.pt.fl_str_mv AvaliaÃÃo in vitro da efetividade de diferentes pastas antibiÃticas utilizadas para curativos endodÃnticos sobre o E. Faecalis
title Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
spellingShingle Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
Adriana Kelly de Sousa Santiago
Pasta antibiÃtica
RegeneraÃÃo endodÃntica
ApicificaÃÃo
Pasta antibiotic
endodontic regeneration
apexification
endodontics
antibiotics
Enterococcus faecalis
ODONTOPEDIATRIA
title_short Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
title_full Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
title_fullStr Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
title_full_unstemmed Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
title_sort Evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis
author Adriana Kelly de Sousa Santiago
author_facet Adriana Kelly de Sousa Santiago
author_role author
dc.contributor.advisor1.fl_str_mv Josà Jeovà Siebra Moreira Neto
dc.contributor.advisor1ID.fl_str_mv 39125432320
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4773258P9
dc.contributor.advisor-co1.fl_str_mv Francisco Fabio Oliveira de Sousa
dc.contributor.advisor-co1ID.fl_str_mv 92923844330
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4777925P5
dc.contributor.referee1.fl_str_mv MÃnica Sampaio do Vale
dc.contributor.referee1ID.fl_str_mv 23010134304
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4786660H3
dc.contributor.referee2.fl_str_mv ClÃudio Maniglia Ferreira
dc.contributor.referee2ID.fl_str_mv 14262563880
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1944193921428069
dc.contributor.authorID.fl_str_mv 00462817385
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1342838696403276
dc.contributor.author.fl_str_mv Adriana Kelly de Sousa Santiago
contributor_str_mv Josà Jeovà Siebra Moreira Neto
Francisco Fabio Oliveira de Sousa
MÃnica Sampaio do Vale
ClÃudio Maniglia Ferreira
dc.subject.por.fl_str_mv Pasta antibiÃtica
RegeneraÃÃo endodÃntica
ApicificaÃÃo
topic Pasta antibiÃtica
RegeneraÃÃo endodÃntica
ApicificaÃÃo
Pasta antibiotic
endodontic regeneration
apexification
endodontics
antibiotics
Enterococcus faecalis
ODONTOPEDIATRIA
dc.subject.eng.fl_str_mv Pasta antibiotic
endodontic regeneration
apexification
endodontics
antibiotics
Enterococcus faecalis
dc.subject.cnpq.fl_str_mv ODONTOPEDIATRIA
dc.description.sponsorship.fl_txt_mv CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
dc.description.abstract.por.fl_txt_mv There is an agreement that in dental treatments with incomplete rizogenese it is necessary an intracanal medication with high antibacterial effect because the use of files and standard protocol based on instrumentation must be avoided to protect the fragile dental element from extra hazard. Several endodontic dressings have been used with this purpose, alone or associated to a copious irrigation procedure. However, it is not yet standardized in the literature a unique medication with protracted and excellent antimicrobial effect, associated to a feasible handling and insertion into the duct. The purpose of this study was to evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis by means of agar disk diffusion method. Accordingly, it was created a polypropylene device to simulate the release characteristics of the main root canal. The efficacy tests were performed over a period of 30 days. The formulations studied were distributed in triplicate just as follows: Triantibiotic Paste (metronidazole, ciprofloxacin and minocycline); Biantibiotic Paste (metronidazole and ciprofloxacin); Ciprofloxacin Paste; Amoxilin Paste; Calcium hydroxide paste; 0.9% saline (control group). All pastes were still allotted into 2 categories: open and closed apex. The devices remained in the medium at 37ÂC over 30 days (static). Samples used for the measurement of biologic response were collected in pre-fixed times: H1(1st hour), H6(6th hour), H24(24th hour), D3(3rd day), D7(7th day), D14(14th day) and D30(30th day) in the course of thirty days. At every settled time, 20μL of solution was removed from each device and embedded on sterile paper discs with 6mm of diameter, being the equivalent volume replaced with fresh release medium. After, the discs were transferred with sterile tweezersg to the surface of agar-BHI medium previously inoculated with bacteria over petri plaques. From the devices with closed apex, it was removed 20μL of solution from above the paste surface, avoiding any directly touch on it. This collect aimed to evaluate the antimicrobial effect of different preparations inside the root canal. From the devices with open apex it was removed 20μL of the solution in which the plastic device was submerged, in order to measure the action of the diffused antibiotic to the periapical region through the apex. From the inhibition halos obtained in each case it was possible to rank the formulations in order of effectiveness. The Amoxicillin paste presented an initial antimicrobial effect more robust, keeping it throughout the experiment. However from the second week there was no more significant difference between this and the other antibiotic pastes (triantibiotic and biantibiotic). These pastes had an initial effect less representative than amoxicillin, however the effect became similar from the second week. Nevertheless, calcium hydroxide paste had a discreet effect initially and it was completely ceased already after the 3rd day. Even if Amoxicillin paste presented a superior result among the studied pastes, it was noticed color changes associated to degradation signs after two weeks. The findings show that these antibiotics pastes posed an excellent pharmacological effect, apart from calcium hydroxide. It can be concluded that all the antibiotics pastes, among them the pastes of amoxicillin, triantibiotic and biantibiotic were effective against E. faecalis. As a result its use as dressing for teeth with incomplete risogenesis in may be a good alternative. Among the advantages it is the microbial effectiveness, as well as the feasible manipulation during the clinical procedure. When comparing biantibiotic and triantibiotic pastes, the first showed to be almost equally effective, being a great choice for the clinical treatments of anterior teeth, where the pigmentation caused by minocycline antibiotic could present a drawback in the final result of the treatment.
Existe um consenso de que no tratamento de dentes com rizogÃnese incompleta à necessÃria uma medicaÃÃo intracanal com mÃximo efeito antibacteriano, pois o uso de limas e o protocolo convencional de instrumentaÃÃo devem ser evitados para que nÃo torne o elemento dental ainda mais frÃgil. Diversos curativos endodÃnticos tÃm sido utilizados com este objetivo, bem como o uso de uma irrigaÃÃo mais copiosa e com irrigante mais efetivo. No entanto, ainda nÃo està padronizada na literatura uma medicaÃÃo com efeito antibiÃtico prolongado e efetivo, alÃm de fÃcil manipulaÃÃo e inserÃÃo no conduto. O objetivo deste trabalho foi avaliar in vitro a eficÃcia de diferentes pastas antimicrobianas utilizadas como medicaÃÃo intracanal sobre o patÃgeno Enterococcus faecalis utilizando o mÃtodo de disco - difusÃo em Ãgar. Para isso, foi confeccionado um dispositivo de polipropileno que possui caracterÃsticas semelhantes ao canal radicular principal. Os testes de eficÃcia foram realizados por um perÃodo de 30 dias. Foram analisadas as seguintes formulaÃÃes: Pasta triantibiÃtica (metronidazol, ciprofloxacino e minociclina); Pasta biantibiÃtica (metronidazol e ciprofloxacino); Pasta de Ciprofloxacino; Pasta de Amoxicilina; Pasta de hidrÃxido de cÃlcio; SoluÃÃo fisiolÃgica 0,9% (grupo controle). Todas as pastas foram ainda alocadas em duas categorias: Ãpice aberto e Ãpice fechado e ensaiadas em triplicata. Os dispositivos permaneceram em estufa a 37ÂC durante 30 dias (sem agitaÃÃo), sendo as coletas para aferiÃÃo da resposta biolÃgica realizadas em perÃodos preestabelecidos: H1(1 hora), H6(6 hora), H24(24 hora), D3(3 dia), D7(7 dia), D14(14 dia) e D30(30 dia) no decorrer de 30 dias. A cada coleta foram retirados de cada dispositivo 20ÂL de soluÃÃo e depositados sobre discos de papel de filtro estÃreis com 6 mm de diÃmetro. Em seguida, os discos de papel embebidos foram transferidos com pinÃa estÃril para a superfÃcie das placas de petri previamente semeadas com a bactÃria. Dos dispositivos com Ãpice fechado foram removidos 20ÂL da soluÃÃo que estava sobre a superfÃcie da pasta, tendo o cuidado para nÃo tocÃ-la. Este procedimento teve o intuito de medir a aÃÃo antimicrobiana direta dos diferentes preparados no interior do conduto radicular. A partir dos dispositivos com Ãpice aberto foram retirados 20ÂL da soluÃÃo na qual o dispositivo plÃstico encontrava-se imerso, com o intuito de medir a aÃÃo do preparado difundido à regiÃo periapical. A partir dos halos de inibiÃÃo obtidos, foi possÃvel observar quais preparados foram mais eficazes com respeito ao seu efeito antibacteriano. A pasta de amoxicilina apresentou o maior efeito antimicrobiano inicial, mantendo-o durante todo o experimento, no entanto, a partir da segunda semana nÃo houve mais diferenÃa estatÃstica entre as pastas antibiÃticas e o efeito tornou-se semelhante. As pastas triantibiÃtica e biantibiÃtica tiveram efeito inicial menor do que o da Amoxicilina, porÃm o efeito foi igualado a partir da segunda semana. Por outro lado, a pasta de hidrÃxido de cÃlcio teve um efeito discreto inicialmente, o qual foi totalmente cessado jà apÃs o 3 dia. Ainda que a pasta de Amoxicilina tenha apresentado os melhores resultados dentre as pastas estudadas, foi evidenciado o aparecimento de cor escurecida associada a sinais de degradaÃÃo apÃs duas semanas. Este estudo mostrou que as pastas antibiÃticas tiveram um excelente efeito farmacolÃgico, ao contrÃrio da pasta de hidrÃxido de cÃlcio. Pode-se concluir que as pastas antibiÃticas, dentre elas as pastas de Amoxicilina, triantibiÃtica e biantibiÃtica apresentaram um excelente efeito sobre o micro-organismo E. faecalis. Portanto, seu uso como medicaÃÃo intracanal nos casos de dentes com rizogÃnese incompleta pode ser uma Ãtima alternativa, estando ainda a facilidade de manipulaÃÃo durante o procedimento clÃnico atrelada à eficÃcia microbicida. Quando comparadas, a pasta biantibiÃtica mostrou-se quase que igualmente efetiva a triantibiÃtica, podendo ser uma Ãtima escolha em casos clÃnicos de tratamento de dentes anteriores, sempre e quando a pigmentaÃÃo causada pelo fÃrmaco Minociclina possa comprometer o resultado final do tratamento. PÃde-se concluir que as pastas antibiÃticas, dentre elas as pastas de amoxicilina, triantibiÃtica e biantibiÃtica apresentaram excelente efeito inibitÃrio sobre o micro-organismo E. faecalis. Portanto, o seu uso como medicaÃÃo intracanal nos casos de dentes com rizogÃnese incompleta pode ser uma excelente alternativa.
description There is an agreement that in dental treatments with incomplete rizogenese it is necessary an intracanal medication with high antibacterial effect because the use of files and standard protocol based on instrumentation must be avoided to protect the fragile dental element from extra hazard. Several endodontic dressings have been used with this purpose, alone or associated to a copious irrigation procedure. However, it is not yet standardized in the literature a unique medication with protracted and excellent antimicrobial effect, associated to a feasible handling and insertion into the duct. The purpose of this study was to evaluate the in vitro effectiveness of different antimicrobial pastes currently used as intracanal medication against the pathogen Enterococcus faecalis by means of agar disk diffusion method. Accordingly, it was created a polypropylene device to simulate the release characteristics of the main root canal. The efficacy tests were performed over a period of 30 days. The formulations studied were distributed in triplicate just as follows: Triantibiotic Paste (metronidazole, ciprofloxacin and minocycline); Biantibiotic Paste (metronidazole and ciprofloxacin); Ciprofloxacin Paste; Amoxilin Paste; Calcium hydroxide paste; 0.9% saline (control group). All pastes were still allotted into 2 categories: open and closed apex. The devices remained in the medium at 37ÂC over 30 days (static). Samples used for the measurement of biologic response were collected in pre-fixed times: H1(1st hour), H6(6th hour), H24(24th hour), D3(3rd day), D7(7th day), D14(14th day) and D30(30th day) in the course of thirty days. At every settled time, 20μL of solution was removed from each device and embedded on sterile paper discs with 6mm of diameter, being the equivalent volume replaced with fresh release medium. After, the discs were transferred with sterile tweezersg to the surface of agar-BHI medium previously inoculated with bacteria over petri plaques. From the devices with closed apex, it was removed 20μL of solution from above the paste surface, avoiding any directly touch on it. This collect aimed to evaluate the antimicrobial effect of different preparations inside the root canal. From the devices with open apex it was removed 20μL of the solution in which the plastic device was submerged, in order to measure the action of the diffused antibiotic to the periapical region through the apex. From the inhibition halos obtained in each case it was possible to rank the formulations in order of effectiveness. The Amoxicillin paste presented an initial antimicrobial effect more robust, keeping it throughout the experiment. However from the second week there was no more significant difference between this and the other antibiotic pastes (triantibiotic and biantibiotic). These pastes had an initial effect less representative than amoxicillin, however the effect became similar from the second week. Nevertheless, calcium hydroxide paste had a discreet effect initially and it was completely ceased already after the 3rd day. Even if Amoxicillin paste presented a superior result among the studied pastes, it was noticed color changes associated to degradation signs after two weeks. The findings show that these antibiotics pastes posed an excellent pharmacological effect, apart from calcium hydroxide. It can be concluded that all the antibiotics pastes, among them the pastes of amoxicillin, triantibiotic and biantibiotic were effective against E. faecalis. As a result its use as dressing for teeth with incomplete risogenesis in may be a good alternative. Among the advantages it is the microbial effectiveness, as well as the feasible manipulation during the clinical procedure. When comparing biantibiotic and triantibiotic pastes, the first showed to be almost equally effective, being a great choice for the clinical treatments of anterior teeth, where the pigmentation caused by minocycline antibiotic could present a drawback in the final result of the treatment.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9471
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9471
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Odontologia
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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