Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16272 |
Resumo: | The study was conducted to evaluate the influence of supplementation of different lipid sources on performance and quantitative characteristics of housing and not housing, fatty acid profile and muscle proteome Longissimus dorsi of Santa Ines lambs fed different additional sources of lipids. 35 lambs bulls were used, with initial body weight (13 Â 1.80 kg) and approximately two months in a randomized block design with five treatments and seven repetitions. The treatments consisted of five diets, one free additional lipids (control) and the other, added cottonseed (CA), bran cashew (FCC), cottonseed with cashew nut meal (CALFCC) and calcium salts of long chain fatty acids (LCFA-Ca). The duration of the experiment was determined by the time required for the average body weight of all animals in each treatment were 28 kg, and this selected group for slaughter. No significant effect was observed with the use of supplementary sources of lipids on the loss of fasting, body weight at slaughter, empty body weight, hot carcass weight, cold carcass weight loss by cooling and biological yield. The non-carcass components, intestines and organs showed no differences across lipid additional sources. Regarding the fatty acid profile were observed 13 fatty acids, four of which are saturated, monounsaturated five-four polyunsaturated (PUFA). In proteome analysis, the proteins were expressed in greater quantity were located primarily at pH less than 5.5. Comparing the expression intensity spots between treatments can observe significant difference in the intensity of the spots 23 for the diet revealed fifteen proteins and of this total, seven were differentiated with respect to time. The proteins identified in this experiment can be mainly classified as structural cellular organization and protection to stress, as well as specific metabolic functions and other functions. The addition of various sources of lipid influenced the productive performance and features: hot carcass dressing and cold, yield and weight of the rack the weight and performance of the gastrointestinal tract filled, small intestine and liver income in Santa Ines sheep growing. Supplementation with different lipid sources influence the profile of fatty acids of Longissimus dorsi, so that diets containing FCC and Lcfa-Ca increased the amount of CLA in the muscle. The proteome analysis may be a method to identify marker proteins meat quality prediction. In this study, the identified proteins are highly relevant to the meat tenderness process, wherein the meat maturation process is complex and involves protein in different biological processes. The type of power had greater influence on the proportions of bodies responsible for digestion and absorption of nutrients. |
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Biblioteca Digital de Teses e Dissertações da UFC |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisEffects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woollessEfeitos de diferentes fontes de lipÃdeos sobre as caracterÃsticas de carcaÃa, perfil lipÃdico e proteoma do Longissimus Dorsi De cordeiros deslanados2014-11-07ElzÃnia Sales Pereira66613965634http://lattes.cnpq.br/4011686548866427Maria Socorro de Souza Carneiro07327307320http://lattes.cnpq.br/6312138252500734Arlindo de Alencar Araripe Moura28462874300http://lattes.cnpq.br/7244663767446774MaurÃcio Fraga van Tilburg03816279775http://lattes.cnpq.br/4336877181545923Silas Primola Gomes82959196600http://lattes.cnpq.br/346259515076303797627720320http://lattes.cnpq.br/958839018913068Paulo Cesar Lopes de ArrudaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo Integrado em Zootecnia-PDIZ UFCBRÃcidos graxosConfinamento proteÃmicaconfinement fatty acids proteomicsZOOTECNIAThe study was conducted to evaluate the influence of supplementation of different lipid sources on performance and quantitative characteristics of housing and not housing, fatty acid profile and muscle proteome Longissimus dorsi of Santa Ines lambs fed different additional sources of lipids. 35 lambs bulls were used, with initial body weight (13  1.80 kg) and approximately two months in a randomized block design with five treatments and seven repetitions. The treatments consisted of five diets, one free additional lipids (control) and the other, added cottonseed (CA), bran cashew (FCC), cottonseed with cashew nut meal (CALFCC) and calcium salts of long chain fatty acids (LCFA-Ca). The duration of the experiment was determined by the time required for the average body weight of all animals in each treatment were 28 kg, and this selected group for slaughter. No significant effect was observed with the use of supplementary sources of lipids on the loss of fasting, body weight at slaughter, empty body weight, hot carcass weight, cold carcass weight loss by cooling and biological yield. The non-carcass components, intestines and organs showed no differences across lipid additional sources. Regarding the fatty acid profile were observed 13 fatty acids, four of which are saturated, monounsaturated five-four polyunsaturated (PUFA). In proteome analysis, the proteins were expressed in greater quantity were located primarily at pH less than 5.5. Comparing the expression intensity spots between treatments can observe significant difference in the intensity of the spots 23 for the diet revealed fifteen proteins and of this total, seven were differentiated with respect to time. The proteins identified in this experiment can be mainly classified as structural cellular organization and protection to stress, as well as specific metabolic functions and other functions. The addition of various sources of lipid influenced the productive performance and features: hot carcass dressing and cold, yield and weight of the rack the weight and performance of the gastrointestinal tract filled, small intestine and liver income in Santa Ines sheep growing. Supplementation with different lipid sources influence the profile of fatty acids of Longissimus dorsi, so that diets containing FCC and Lcfa-Ca increased the amount of CLA in the muscle. The proteome analysis may be a method to identify marker proteins meat quality prediction. In this study, the identified proteins are highly relevant to the meat tenderness process, wherein the meat maturation process is complex and involves protein in different biological processes. The type of power had greater influence on the proportions of bodies responsible for digestion and absorption of nutrients.O estudo foi realizado com objetivo de avaliar a influÃncia da suplementaÃÃo de diferentes fontes de lipÃdeos sobre o desempenho produtivo e as caracterÃsticas quantitativas de carcaÃa e nÃo carcaÃa, perfil de Ãcidos graxos e proteoma do mÃsculo Longissimus dorsi de cordeiros Santa InÃs alimentados com diferentes fontes suplementares de lipÃdeos. Foram utilizados 35 cordeiros nÃo-castrados, com peso corporal mÃdio inicial (13  1,80 kg) e aproximadamente, dois meses de idade em delineamento de blocos ao acaso com cinco tratamentos e sete repetiÃÃes. Os tratamentos experimentais consistiram de cinco raÃÃes, sendo uma isenta de lipÃdeos suplementares (controle) e as demais, adicionadas de caroÃo de algodÃo (CA), farelo da castanha de caju (FCC), caroÃo de algodÃo com farelo de castanha de caju (CALFCC) e sais de cÃlcio de Ãcidos graxos de cadeia longa (Ca-Agcl). A duraÃÃo do experimento foi determinada pelo tempo necessÃrio para que a mÃdia do peso corporal de todos os animais de cada tratamento atingisse 28 kg, sendo este grupo selecionado para o abate. NÃo foi observado efeito significativo com a utilizaÃÃo de fontes suplementares de lipÃdeos sobre a perda do jejum, peso corporal ao abate, peso de corpo vazio, peso de carcaÃa quente, peso de carcaÃa fria, perda por resfriamento e rendimento biolÃgico. Os componentes nÃo-carcaÃa, vÃsceras e ÃrgÃos, nÃo apresentaram diferenÃas em funÃÃo das fontes suplementares lipÃdicas. No tocante ao perfil de Ãcidos graxos foram observados 13 Ãcidos graxos, dos quais quatro sÃo saturados, cinco monoinsaturados e quatro poliinsaturados(AGPI). Na anÃlise do proteÃma as proteÃnas que foram expressas em maior quantidade se localizaram principalmente em pH inferior a 5,5. Comparando a expressÃo da intensidade de spots entre os tratamentos pode-se observar diferenÃa significativa na intensidade de 23 spots em funÃÃo da dieta revelando quinze proteÃnas sendo que desse total, sete foram diferenciadas em funÃÃo do tempo. As proteÃnas identificadas neste experimento podem ser classificadas principalmente como estruturais de organizaÃÃo celular e proteÃÃo ao stresse, bem como com especÃficas funÃÃes metabÃlicas e outras funÃÃes. A adiÃÃo de diferentes fontes de lipÃdeo suplementar influenciou o desempenho produtivo e as caracterÃsticas: rendimento da carcaÃa quente e fria, rendimento e peso da costela o peso e rendimento do trato gastrointestinal cheio, intestino delgado e rendimento do fÃgado em ovinos Santa InÃs em crescimento. A suplementaÃÃo com diferentes fontes lipÃdicas influenciou o perfil de Ãcidos graxos do Longissimus dorsi, de modo que as dietas contendo FCC e Ca-Agcl aumentou a quantidade de CLA neste mÃsculo. A anÃlise do proteoma pode ser um mÃtodo para identificar as proteÃnas marcadoras de prediÃÃo da qualidade da carne. Nesse estudo, as proteÃnas identificadas sÃo altamente relevantes para o processo de maciez da carne, sendo que o processo de maturaÃÃo da carne à complexo e envolve proteÃnas em diferentes processos biolÃgicos. O tipo de alimentaÃÃo teve maior influÃncia sobre as proporÃÃes dos ÃrgÃos responsÃveis pela digestÃo e absorÃÃo de nutrientes.FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicohttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16272application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:29:27Zmail@mail.com - |
dc.title.en.fl_str_mv |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
dc.title.alternative.pt.fl_str_mv |
Efeitos de diferentes fontes de lipÃdeos sobre as caracterÃsticas de carcaÃa, perfil lipÃdico e proteoma do Longissimus Dorsi De cordeiros deslanados |
title |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
spellingShingle |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless Paulo Cesar Lopes de Arruda Ãcidos graxos Confinamento proteÃmica confinement fatty acids proteomics ZOOTECNIA |
title_short |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
title_full |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
title_fullStr |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
title_full_unstemmed |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
title_sort |
Effects of different lipid sources on housing characteristics, lipid profile and proteome Longissimus Dorsi lambs woolless |
author |
Paulo Cesar Lopes de Arruda |
author_facet |
Paulo Cesar Lopes de Arruda |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
ElzÃnia Sales Pereira |
dc.contributor.advisor1ID.fl_str_mv |
66613965634 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4011686548866427 |
dc.contributor.referee1.fl_str_mv |
Maria Socorro de Souza Carneiro |
dc.contributor.referee1ID.fl_str_mv |
07327307320 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6312138252500734 |
dc.contributor.referee2.fl_str_mv |
Arlindo de Alencar Araripe Moura |
dc.contributor.referee2ID.fl_str_mv |
28462874300 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7244663767446774 |
dc.contributor.referee3.fl_str_mv |
MaurÃcio Fraga van Tilburg |
dc.contributor.referee3ID.fl_str_mv |
03816279775 |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4336877181545923 |
dc.contributor.referee4.fl_str_mv |
Silas Primola Gomes |
dc.contributor.referee4ID.fl_str_mv |
82959196600 |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/3462595150763037 |
dc.contributor.authorID.fl_str_mv |
97627720320 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/958839018913068 |
dc.contributor.author.fl_str_mv |
Paulo Cesar Lopes de Arruda |
contributor_str_mv |
ElzÃnia Sales Pereira Maria Socorro de Souza Carneiro Arlindo de Alencar Araripe Moura MaurÃcio Fraga van Tilburg Silas Primola Gomes |
dc.subject.por.fl_str_mv |
Ãcidos graxos Confinamento proteÃmica |
topic |
Ãcidos graxos Confinamento proteÃmica confinement fatty acids proteomics ZOOTECNIA |
dc.subject.eng.fl_str_mv |
confinement fatty acids proteomics |
dc.subject.cnpq.fl_str_mv |
ZOOTECNIA |
dc.description.sponsorship.fl_txt_mv |
FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico |
dc.description.abstract.por.fl_txt_mv |
The study was conducted to evaluate the influence of supplementation of different lipid sources on performance and quantitative characteristics of housing and not housing, fatty acid profile and muscle proteome Longissimus dorsi of Santa Ines lambs fed different additional sources of lipids. 35 lambs bulls were used, with initial body weight (13  1.80 kg) and approximately two months in a randomized block design with five treatments and seven repetitions. The treatments consisted of five diets, one free additional lipids (control) and the other, added cottonseed (CA), bran cashew (FCC), cottonseed with cashew nut meal (CALFCC) and calcium salts of long chain fatty acids (LCFA-Ca). The duration of the experiment was determined by the time required for the average body weight of all animals in each treatment were 28 kg, and this selected group for slaughter. No significant effect was observed with the use of supplementary sources of lipids on the loss of fasting, body weight at slaughter, empty body weight, hot carcass weight, cold carcass weight loss by cooling and biological yield. The non-carcass components, intestines and organs showed no differences across lipid additional sources. Regarding the fatty acid profile were observed 13 fatty acids, four of which are saturated, monounsaturated five-four polyunsaturated (PUFA). In proteome analysis, the proteins were expressed in greater quantity were located primarily at pH less than 5.5. Comparing the expression intensity spots between treatments can observe significant difference in the intensity of the spots 23 for the diet revealed fifteen proteins and of this total, seven were differentiated with respect to time. The proteins identified in this experiment can be mainly classified as structural cellular organization and protection to stress, as well as specific metabolic functions and other functions. The addition of various sources of lipid influenced the productive performance and features: hot carcass dressing and cold, yield and weight of the rack the weight and performance of the gastrointestinal tract filled, small intestine and liver income in Santa Ines sheep growing. Supplementation with different lipid sources influence the profile of fatty acids of Longissimus dorsi, so that diets containing FCC and Lcfa-Ca increased the amount of CLA in the muscle. The proteome analysis may be a method to identify marker proteins meat quality prediction. In this study, the identified proteins are highly relevant to the meat tenderness process, wherein the meat maturation process is complex and involves protein in different biological processes. The type of power had greater influence on the proportions of bodies responsible for digestion and absorption of nutrients. O estudo foi realizado com objetivo de avaliar a influÃncia da suplementaÃÃo de diferentes fontes de lipÃdeos sobre o desempenho produtivo e as caracterÃsticas quantitativas de carcaÃa e nÃo carcaÃa, perfil de Ãcidos graxos e proteoma do mÃsculo Longissimus dorsi de cordeiros Santa InÃs alimentados com diferentes fontes suplementares de lipÃdeos. Foram utilizados 35 cordeiros nÃo-castrados, com peso corporal mÃdio inicial (13  1,80 kg) e aproximadamente, dois meses de idade em delineamento de blocos ao acaso com cinco tratamentos e sete repetiÃÃes. Os tratamentos experimentais consistiram de cinco raÃÃes, sendo uma isenta de lipÃdeos suplementares (controle) e as demais, adicionadas de caroÃo de algodÃo (CA), farelo da castanha de caju (FCC), caroÃo de algodÃo com farelo de castanha de caju (CALFCC) e sais de cÃlcio de Ãcidos graxos de cadeia longa (Ca-Agcl). A duraÃÃo do experimento foi determinada pelo tempo necessÃrio para que a mÃdia do peso corporal de todos os animais de cada tratamento atingisse 28 kg, sendo este grupo selecionado para o abate. NÃo foi observado efeito significativo com a utilizaÃÃo de fontes suplementares de lipÃdeos sobre a perda do jejum, peso corporal ao abate, peso de corpo vazio, peso de carcaÃa quente, peso de carcaÃa fria, perda por resfriamento e rendimento biolÃgico. Os componentes nÃo-carcaÃa, vÃsceras e ÃrgÃos, nÃo apresentaram diferenÃas em funÃÃo das fontes suplementares lipÃdicas. No tocante ao perfil de Ãcidos graxos foram observados 13 Ãcidos graxos, dos quais quatro sÃo saturados, cinco monoinsaturados e quatro poliinsaturados(AGPI). Na anÃlise do proteÃma as proteÃnas que foram expressas em maior quantidade se localizaram principalmente em pH inferior a 5,5. Comparando a expressÃo da intensidade de spots entre os tratamentos pode-se observar diferenÃa significativa na intensidade de 23 spots em funÃÃo da dieta revelando quinze proteÃnas sendo que desse total, sete foram diferenciadas em funÃÃo do tempo. As proteÃnas identificadas neste experimento podem ser classificadas principalmente como estruturais de organizaÃÃo celular e proteÃÃo ao stresse, bem como com especÃficas funÃÃes metabÃlicas e outras funÃÃes. A adiÃÃo de diferentes fontes de lipÃdeo suplementar influenciou o desempenho produtivo e as caracterÃsticas: rendimento da carcaÃa quente e fria, rendimento e peso da costela o peso e rendimento do trato gastrointestinal cheio, intestino delgado e rendimento do fÃgado em ovinos Santa InÃs em crescimento. A suplementaÃÃo com diferentes fontes lipÃdicas influenciou o perfil de Ãcidos graxos do Longissimus dorsi, de modo que as dietas contendo FCC e Ca-Agcl aumentou a quantidade de CLA neste mÃsculo. A anÃlise do proteoma pode ser um mÃtodo para identificar as proteÃnas marcadoras de prediÃÃo da qualidade da carne. Nesse estudo, as proteÃnas identificadas sÃo altamente relevantes para o processo de maciez da carne, sendo que o processo de maturaÃÃo da carne à complexo e envolve proteÃnas em diferentes processos biolÃgicos. O tipo de alimentaÃÃo teve maior influÃncia sobre as proporÃÃes dos ÃrgÃos responsÃveis pela digestÃo e absorÃÃo de nutrientes. |
description |
The study was conducted to evaluate the influence of supplementation of different lipid sources on performance and quantitative characteristics of housing and not housing, fatty acid profile and muscle proteome Longissimus dorsi of Santa Ines lambs fed different additional sources of lipids. 35 lambs bulls were used, with initial body weight (13 Â 1.80 kg) and approximately two months in a randomized block design with five treatments and seven repetitions. The treatments consisted of five diets, one free additional lipids (control) and the other, added cottonseed (CA), bran cashew (FCC), cottonseed with cashew nut meal (CALFCC) and calcium salts of long chain fatty acids (LCFA-Ca). The duration of the experiment was determined by the time required for the average body weight of all animals in each treatment were 28 kg, and this selected group for slaughter. No significant effect was observed with the use of supplementary sources of lipids on the loss of fasting, body weight at slaughter, empty body weight, hot carcass weight, cold carcass weight loss by cooling and biological yield. The non-carcass components, intestines and organs showed no differences across lipid additional sources. Regarding the fatty acid profile were observed 13 fatty acids, four of which are saturated, monounsaturated five-four polyunsaturated (PUFA). In proteome analysis, the proteins were expressed in greater quantity were located primarily at pH less than 5.5. Comparing the expression intensity spots between treatments can observe significant difference in the intensity of the spots 23 for the diet revealed fifteen proteins and of this total, seven were differentiated with respect to time. The proteins identified in this experiment can be mainly classified as structural cellular organization and protection to stress, as well as specific metabolic functions and other functions. The addition of various sources of lipid influenced the productive performance and features: hot carcass dressing and cold, yield and weight of the rack the weight and performance of the gastrointestinal tract filled, small intestine and liver income in Santa Ines sheep growing. Supplementation with different lipid sources influence the profile of fatty acids of Longissimus dorsi, so that diets containing FCC and Lcfa-Ca increased the amount of CLA in the muscle. The proteome analysis may be a method to identify marker proteins meat quality prediction. In this study, the identified proteins are highly relevant to the meat tenderness process, wherein the meat maturation process is complex and involves protein in different biological processes. The type of power had greater influence on the proportions of bodies responsible for digestion and absorption of nutrients. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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publishedVersion |
format |
doctoralThesis |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16272 |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16272 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo Integrado em Zootecnia-PDIZ |
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UFC |
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BR |
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Universidade Federal do Cearà |
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reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
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UFC |
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