Eletrophysiological evaluation of guanylin and urogunylin in rat brain
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Tipo de documento: | Dissertação |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
| Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14 |
Resumo: | Guanylin and uroguanylin are heat-stable peptides isolated and identified from rat intestine and opossum urine, respectively. They control salt and water transport in the kidney and intestine mediated by cGMP. In this study we tried to show the effects of the guanylin-like peptides on EEG-parameters, as well to investigate possible cerebral action mechanisms in the central nervous system. The experiments were performed using anaesthetized male Wistar rats that were placed on the stereotaxic frame for surgery to implant a guide cannula towards to cisterna magna. After 48 hours, the animals were divided in three groups: guanylin (2μg/μl/min) and uroguanylin (2μg/μl/min and 6μg/μl/min), and recived intracisternal infusion by a infusion pump. Another two groups were performed using uroguanylin (2μg/μl/min) and a pretreatment of two Clˉ blockers: niflumic acid and nedocromil sodium. EEG recordings were made throughout the experimental procedure, using a software for spectral activity study and absolute amplitude, starting with the control recording segment, followed by drug infusion segment and finishing with after infusion segment. Guanylin peptide in the rat brain increased the frontal waves amplitude and induced spikes. Uroguanylin induced the same changes more intensively (p<0.05). Niflumic acid didnât promoted changes, but nedocromil seemed to inhibit the spikes (p<0.05). We propose that guanylin and uroguanilyn EEG effects were caused by Clˉ channels envolvement. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEletrophysiological evaluation of guanylin and urogunylin in rat brainAvaliaÃÃo eletrofisiolÃgica da aÃÃo da guanilina e de uroguanilina em cÃrebro de ratos2003-10-13ManassÃs Claudino Fonteles00251135349http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4783570A5Francisca ClÃa FlorenÃo de Sousa31636020372http://lattes.cnpq.br/1180465052181572Otoni Cardoso do Vale01404229353http://lattes.cnpq.br/0128019591224851 46382992372http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4799337P0Maria Daniele Azevedo TeixeiraUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em FarmacologiaUFCBREletroencefalografia Guanilina Uroguanilina Canais de Cloreto NerofarmacologiaElectroencephalogram Guanylin Uroguanylin Chloride channel NeuropharmacologyNEUROFISIOLOGIAGuanylin and uroguanylin are heat-stable peptides isolated and identified from rat intestine and opossum urine, respectively. They control salt and water transport in the kidney and intestine mediated by cGMP. In this study we tried to show the effects of the guanylin-like peptides on EEG-parameters, as well to investigate possible cerebral action mechanisms in the central nervous system. The experiments were performed using anaesthetized male Wistar rats that were placed on the stereotaxic frame for surgery to implant a guide cannula towards to cisterna magna. After 48 hours, the animals were divided in three groups: guanylin (2μg/μl/min) and uroguanylin (2μg/μl/min and 6μg/μl/min), and recived intracisternal infusion by a infusion pump. Another two groups were performed using uroguanylin (2μg/μl/min) and a pretreatment of two Clˉ blockers: niflumic acid and nedocromil sodium. EEG recordings were made throughout the experimental procedure, using a software for spectral activity study and absolute amplitude, starting with the control recording segment, followed by drug infusion segment and finishing with after infusion segment. Guanylin peptide in the rat brain increased the frontal waves amplitude and induced spikes. Uroguanylin induced the same changes more intensively (p<0.05). Niflumic acid didnât promoted changes, but nedocromil seemed to inhibit the spikes (p<0.05). We propose that guanylin and uroguanilyn EEG effects were caused by Clˉ channels envolvement. Os peptÃdeos termo-estÃveis guanilina e uroguanilina foram inicialmente isolados e identificados do intestino de rato e de urina de opossum: suas propriedades sÃo atribuÃdas ao controle do transporte de sal e Ãgua no rim e intestino, mediado pelo GMPc. O presente estudo propÃe-se a avaliar a atividade neurofisiolÃgica dos peptÃdeos do tipo guanilina, atravÃs da anÃlise do registro eletroencefÃlico, bem como investigar os mecanismos de aÃÃo responsÃveis pela possÃvel aÃÃo sobre o sistema nervoso central. Para tanto, grupos de ratos Wistar machos anestesiados foram submetidos a uma cirurgia para a colocaÃÃo de uma cÃnula na cisterna magna. Decorridas 48 horas da cirurgia, estes animais foram novamente anestesiados, sendo infundidas atravÃs de uma bomba de infusÃo: guanilina (2μg/μl/min) e uroguanilina (2μg/μl/mim e 6μg/μl/min), em trÃs grupos distintos. Posteriormente, outros dois grupos de animais foram submetidos ao mesmo protocolo experimental, com a uroguanilina, porÃm adicionalmente, receberam um prÃ-tratamento (antes da infusÃo) de duas substÃncias bloqueadoras de canais de Clˉ: o Ãcido niflÃmico e o nedocromil sÃdico. Durante a infusÃo intracisternal dos peptÃdeos, houve o registro do EEG dos diversos espectros de ondas, sendo gravados trÃs momentos: antes da infusÃo ( controle), durante e apÃs a infusÃo. O peptÃdeo guanilina quando infundido em cÃrebro de ratos levou a alteraÃÃes na amplitude do traÃado e o surgimento de pontas no EEG. A uroguanilina induziu as mesmas alteraÃÃes, contudo houve uma maior intensidade (p<0.05). O prÃ-tratamento com Ãcido niflÃmico nÃo influiu nos resultados da infusÃo de uroguanilina, porÃm o nedocromil inibiu o surgimento de pontas (p<0.05). Sugerimos atravÃs deste estudo, que os peptÃdeos guanilina e uroguanilina produzem alteraÃÃes eletroencefalogrÃficas, atuando sobre o cÃrebro por mecanismos de aÃÃo envolvendo canais de Clˉ. FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicoCoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:13:06Zmail@mail.com - |
| dc.title..fl_str_mv |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| dc.title.alternative.pt.fl_str_mv |
AvaliaÃÃo eletrofisiolÃgica da aÃÃo da guanilina e de uroguanilina em cÃrebro de ratos |
| title |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| spellingShingle |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain Maria Daniele Azevedo Teixeira Eletroencefalografia Guanilina Uroguanilina Canais de Cloreto Nerofarmacologia Electroencephalogram Guanylin Uroguanylin Chloride channel Neuropharmacology NEUROFISIOLOGIA |
| title_short |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| title_full |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| title_fullStr |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| title_full_unstemmed |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| title_sort |
Eletrophysiological evaluation of guanylin and urogunylin in rat brain |
| author |
Maria Daniele Azevedo Teixeira |
| author_facet |
Maria Daniele Azevedo Teixeira |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
ManassÃs Claudino Fonteles |
| dc.contributor.advisor1ID.fl_str_mv |
00251135349 |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4783570A5 |
| dc.contributor.referee1.fl_str_mv |
Francisca ClÃa FlorenÃo de Sousa |
| dc.contributor.referee1ID.fl_str_mv |
31636020372 |
| dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1180465052181572 |
| dc.contributor.referee2.fl_str_mv |
Otoni Cardoso do Vale |
| dc.contributor.referee2ID.fl_str_mv |
01404229353 |
| dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0128019591224851 |
| dc.contributor.authorID.fl_str_mv |
46382992372 |
| dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4799337P0 |
| dc.contributor.author.fl_str_mv |
Maria Daniele Azevedo Teixeira |
| contributor_str_mv |
ManassÃs Claudino Fonteles Francisca ClÃa FlorenÃo de Sousa Otoni Cardoso do Vale |
| dc.subject.por.fl_str_mv |
Eletroencefalografia Guanilina Uroguanilina Canais de Cloreto Nerofarmacologia |
| topic |
Eletroencefalografia Guanilina Uroguanilina Canais de Cloreto Nerofarmacologia Electroencephalogram Guanylin Uroguanylin Chloride channel Neuropharmacology NEUROFISIOLOGIA |
| dc.subject.eng.fl_str_mv |
Electroencephalogram Guanylin Uroguanylin Chloride channel Neuropharmacology |
| dc.subject.cnpq.fl_str_mv |
NEUROFISIOLOGIA |
| dc.description.sponsorship.fl_txt_mv |
FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior |
| dc.description.abstract.por.fl_txt_mv |
Guanylin and uroguanylin are heat-stable peptides isolated and identified from rat intestine and opossum urine, respectively. They control salt and water transport in the kidney and intestine mediated by cGMP. In this study we tried to show the effects of the guanylin-like peptides on EEG-parameters, as well to investigate possible cerebral action mechanisms in the central nervous system. The experiments were performed using anaesthetized male Wistar rats that were placed on the stereotaxic frame for surgery to implant a guide cannula towards to cisterna magna. After 48 hours, the animals were divided in three groups: guanylin (2μg/μl/min) and uroguanylin (2μg/μl/min and 6μg/μl/min), and recived intracisternal infusion by a infusion pump. Another two groups were performed using uroguanylin (2μg/μl/min) and a pretreatment of two Clˉ blockers: niflumic acid and nedocromil sodium. EEG recordings were made throughout the experimental procedure, using a software for spectral activity study and absolute amplitude, starting with the control recording segment, followed by drug infusion segment and finishing with after infusion segment. Guanylin peptide in the rat brain increased the frontal waves amplitude and induced spikes. Uroguanylin induced the same changes more intensively (p<0.05). Niflumic acid didnât promoted changes, but nedocromil seemed to inhibit the spikes (p<0.05). We propose that guanylin and uroguanilyn EEG effects were caused by Clˉ channels envolvement. Os peptÃdeos termo-estÃveis guanilina e uroguanilina foram inicialmente isolados e identificados do intestino de rato e de urina de opossum: suas propriedades sÃo atribuÃdas ao controle do transporte de sal e Ãgua no rim e intestino, mediado pelo GMPc. O presente estudo propÃe-se a avaliar a atividade neurofisiolÃgica dos peptÃdeos do tipo guanilina, atravÃs da anÃlise do registro eletroencefÃlico, bem como investigar os mecanismos de aÃÃo responsÃveis pela possÃvel aÃÃo sobre o sistema nervoso central. Para tanto, grupos de ratos Wistar machos anestesiados foram submetidos a uma cirurgia para a colocaÃÃo de uma cÃnula na cisterna magna. Decorridas 48 horas da cirurgia, estes animais foram novamente anestesiados, sendo infundidas atravÃs de uma bomba de infusÃo: guanilina (2μg/μl/min) e uroguanilina (2μg/μl/mim e 6μg/μl/min), em trÃs grupos distintos. Posteriormente, outros dois grupos de animais foram submetidos ao mesmo protocolo experimental, com a uroguanilina, porÃm adicionalmente, receberam um prÃ-tratamento (antes da infusÃo) de duas substÃncias bloqueadoras de canais de Clˉ: o Ãcido niflÃmico e o nedocromil sÃdico. Durante a infusÃo intracisternal dos peptÃdeos, houve o registro do EEG dos diversos espectros de ondas, sendo gravados trÃs momentos: antes da infusÃo ( controle), durante e apÃs a infusÃo. O peptÃdeo guanilina quando infundido em cÃrebro de ratos levou a alteraÃÃes na amplitude do traÃado e o surgimento de pontas no EEG. A uroguanilina induziu as mesmas alteraÃÃes, contudo houve uma maior intensidade (p<0.05). O prÃ-tratamento com Ãcido niflÃmico nÃo influiu nos resultados da infusÃo de uroguanilina, porÃm o nedocromil inibiu o surgimento de pontas (p<0.05). Sugerimos atravÃs deste estudo, que os peptÃdeos guanilina e uroguanilina produzem alteraÃÃes eletroencefalogrÃficas, atuando sobre o cÃrebro por mecanismos de aÃÃo envolvendo canais de Clˉ. |
| description |
Guanylin and uroguanylin are heat-stable peptides isolated and identified from rat intestine and opossum urine, respectively. They control salt and water transport in the kidney and intestine mediated by cGMP. In this study we tried to show the effects of the guanylin-like peptides on EEG-parameters, as well to investigate possible cerebral action mechanisms in the central nervous system. The experiments were performed using anaesthetized male Wistar rats that were placed on the stereotaxic frame for surgery to implant a guide cannula towards to cisterna magna. After 48 hours, the animals were divided in three groups: guanylin (2μg/μl/min) and uroguanylin (2μg/μl/min and 6μg/μl/min), and recived intracisternal infusion by a infusion pump. Another two groups were performed using uroguanylin (2μg/μl/min) and a pretreatment of two Clˉ blockers: niflumic acid and nedocromil sodium. EEG recordings were made throughout the experimental procedure, using a software for spectral activity study and absolute amplitude, starting with the control recording segment, followed by drug infusion segment and finishing with after infusion segment. Guanylin peptide in the rat brain increased the frontal waves amplitude and induced spikes. Uroguanylin induced the same changes more intensively (p<0.05). Niflumic acid didnât promoted changes, but nedocromil seemed to inhibit the spikes (p<0.05). We propose that guanylin and uroguanilyn EEG effects were caused by Clˉ channels envolvement. |
| publishDate |
2003 |
| dc.date.issued.fl_str_mv |
2003-10-13 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal do Cearà |
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Programa de PÃs-GraduaÃÃo em Farmacologia |
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UFC |
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BR |
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Universidade Federal do Cearà |
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