Development and method validation for multiresidue determination of pesticides in mango (Mangifera indica L.) using QuEChERS and GC - MS

• Autor(a) principal: FÃtima Itana Chaves CustÃdio Martins
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
• Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15591
• topic: Pesticidas; Cromatografia; Frutos; Pesticides; Chromatography; Fruits; QUIMICA ANALITICA

The development of analytical methods aiming at monitoring of pesticides is essential to ensure that foods are within limits considered safe for health, and to enable the regulatory agencies to obtain faster and more reliable results. In this work, we developed and validated a method for the determination of pesticides in mango, running the sample preparation with QuEChERS method modified with the addition of graphitized carbon (CGB - Carbon Grafitized Black) to remove nonpolar pigments and to identify and quantification of the compounds to the Gas Chromatography Mass Spectrometry (GC-MS). The developed method showed selectivity for all compounds. For all compounds was identified matrix effect, so the study of quantification of the compound was made based on the curves made in the matrix extract. The limits of detection (LOD) ranged from 0,0025 to 0,01 mg.kg-1 and the limits of quantification (LOQ) ranged from 0,008 to 0,03 mg.kg-1. The compounds showed acceptable recovery levels ranging from34% to 143%. The method was applied to the determination of pesticide residues in twelve samples of mango, among which, in six samples were detected five different compounds (Chloroneb, Propachlor, α-Chlordane, Chlorpyrifos,DCPA, Chlorobenzilate and trans-Permethrin). For Chloroneb compounds, Propachlor and α-Chlordane were detected concentration above the maximum residue limit allowed under EU data