Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - CearÃ
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Tipo de documento: | Dissertação |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
| Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13386 |
Resumo: | This research work was designed to detect the presence of Aeromonas in the Cocà River estuary, Fortaleza, Cearà State, Brazil. The database consisted of 30 samples of the riverâs surface water and 30 samples of the riverâs sooil, in the period from September, 2007 to April, 2008.. They were amenable, simultaneously, to counting of bacteria on Agar Gelatin Phosphate Salt (GSP) plus 20μg/mL of ampicilim (UFC/mL or UFC/g) The results showed dissemination of Aeromonas in the estuary. The counts for the water samples varied from 10 to 7,050 and from 25 to 38,500 UFC/mL at points A and B respectively; and from 100 to 37,500 UFC/g, and 1,200 to 43500 UFC/g at points A and B respectively. At point C, the counts for water and sediment were smaller than 10 UFC per ml or gram in all samples The occurrence of the greatest indices of Aeromonas in April, at the height of the rainy season, and the lowest in Septemer, at the height of the dry season, suggests there to be a probable seasonality of bacteria density in the studied environment. Among the 41 isolated strains were found the species A. caviae, A. sobria, A. trota, A. salmonicida and A. allossacharophyla. All the strains of Aeromonas sp. were found to be sensitive to cloranfenicol and ceftriaxona except for ampicillim, to which they showed 100% resistance.All the stirps showed resistance to two out of the nine tested antibiotics. After the Curingâ technique, the eritromicina resistance seems to be of plasmidian origin. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDetection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - CearÃDetecÃÃo de Aeromonas spp. em amostras de Ãgua superficial e sedimento ao longo de um gradiente de salinidade no estuÃrio do Rio Cocà - CearÃ2009-04-24Regine Helena Silva dos Fernandes Vieira09061029368Oscarina Viana de Sousa38583933391http://lattes.cnpq.br/6529999796909142Norma Suely Evangelista Barreto35547057304http://lattes.cnpq.br/910036226937245162255851334http://lattes.cnpq.br/8744370708180407 Camila MagalhÃes SilvaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Engenharia de PescaUFCBRAeromonas Ambientes aquÃticos ResistÃnciaCura do plasmÃdio AntibioticsPlasmids Aquatic environmentENGENHARIA DE PESCAThis research work was designed to detect the presence of Aeromonas in the Cocà River estuary, Fortaleza, Cearà State, Brazil. The database consisted of 30 samples of the riverâs surface water and 30 samples of the riverâs sooil, in the period from September, 2007 to April, 2008.. They were amenable, simultaneously, to counting of bacteria on Agar Gelatin Phosphate Salt (GSP) plus 20μg/mL of ampicilim (UFC/mL or UFC/g) The results showed dissemination of Aeromonas in the estuary. The counts for the water samples varied from 10 to 7,050 and from 25 to 38,500 UFC/mL at points A and B respectively; and from 100 to 37,500 UFC/g, and 1,200 to 43500 UFC/g at points A and B respectively. At point C, the counts for water and sediment were smaller than 10 UFC per ml or gram in all samples The occurrence of the greatest indices of Aeromonas in April, at the height of the rainy season, and the lowest in Septemer, at the height of the dry season, suggests there to be a probable seasonality of bacteria density in the studied environment. Among the 41 isolated strains were found the species A. caviae, A. sobria, A. trota, A. salmonicida and A. allossacharophyla. All the strains of Aeromonas sp. were found to be sensitive to cloranfenicol and ceftriaxona except for ampicillim, to which they showed 100% resistance.All the stirps showed resistance to two out of the nine tested antibiotics. After the Curingâ technique, the eritromicina resistance seems to be of plasmidian origin.Este projeto teve como objetivo principal a pesquisa de Aeromonas spp. em Ãgua de superfÃcie e sedimento em trÃs pontos distintos ao longo do rio CocÃ, CearÃ. Foram realizadas coletas no perÃodo de outubro de 2007 a abril de 2008 gerando um total de 30 amostras de Ãgua e 30 de sedimento. A quantificaÃÃo da comunidade bacteriana pertencente ao gÃnero Aeromonas foi feita atravÃs de plaqueamento direto sobre Agar Gelatina Fosfato Sal (Agar GSP acrescido de 20μg/mL de ampicilina). Nas amostras de Ãgua, os valores obtidos variaram de 10 a 7.050 UFC/mL e de 25 a 38.500 UFC/mL nos pontos A e B, respectivamente. Nas amostras de sedimento, as contagens variaram de 100 a 37500 UFC/g e 1.200 a 43.500 UFC/g nos pontos A e B, respectivamente. Nas amostras de Ãgua e sedimento do ponto C, os valores foram menores que 10 UFC (por mL ou g) em todas as coletas. As maiores contagens foram verificadas no mÃs de abril, perÃodo de chuva, e as menores no mÃs de setembro, perÃodo de estiagem. Foram feitos isolamentos e apÃs identificaÃÃo as estirpes foram submetidas à teste de antibiograma e à tÃcnica da âcuraâ do plasmÃdio. Dentre as 41 cepas isoladas, foram identificadas as espÃcies A. caviae, A. sobria, A. trota, A. salmonicida e A. allossacharophyla. Todas as cepas se mostraram sensÃveis ao cloranfenicol e ceftriaxona. Todas as estirpes apresentaram resistÃncia a, pelo menos, dois dos nove antibiÃticos testados. ApÃs a tÃcnica de cura, a maior parte da resistÃncia a eritromicina ficou caracterizada como de origem plasmidial.Conclui-se que o estuÃrio do Rio Cocà està contaminado por Aeromonas e que muitas delas apresentam resistÃncias a antibiÃticos denotando um ambiente poluÃdo e de risco para a populaÃÃo que usa suas Ãguas para lazer, pesca ou outra atividade qualquer. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13386application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:27:01Zmail@mail.com - |
| dc.title.en.fl_str_mv |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| dc.title.alternative.pt.fl_str_mv |
DetecÃÃo de Aeromonas spp. em amostras de Ãgua superficial e sedimento ao longo de um gradiente de salinidade no estuÃrio do Rio Cocà - Cearà |
| title |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| spellingShingle |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà Camila MagalhÃes Silva Aeromonas Ambientes aquÃticos ResistÃncia Cura do plasmÃdio Antibiotics Plasmids Aquatic environment ENGENHARIA DE PESCA |
| title_short |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| title_full |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| title_fullStr |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| title_full_unstemmed |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| title_sort |
Detection Aeromonas spp. in surface water samples and sediment along a salinity gradient in river estuary Cocà - Cearà |
| author |
Camila MagalhÃes Silva |
| author_facet |
Camila MagalhÃes Silva |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Regine Helena Silva dos Fernandes Vieira |
| dc.contributor.advisor1ID.fl_str_mv |
09061029368 |
| dc.contributor.referee1.fl_str_mv |
Oscarina Viana de Sousa |
| dc.contributor.referee1ID.fl_str_mv |
38583933391 |
| dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6529999796909142 |
| dc.contributor.referee2.fl_str_mv |
Norma Suely Evangelista Barreto |
| dc.contributor.referee2ID.fl_str_mv |
35547057304 |
| dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9100362269372451 |
| dc.contributor.authorID.fl_str_mv |
62255851334 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8744370708180407 |
| dc.contributor.author.fl_str_mv |
Camila MagalhÃes Silva |
| contributor_str_mv |
Regine Helena Silva dos Fernandes Vieira Oscarina Viana de Sousa Norma Suely Evangelista Barreto |
| dc.subject.por.fl_str_mv |
Aeromonas Ambientes aquÃticos ResistÃncia Cura do plasmÃdio |
| topic |
Aeromonas Ambientes aquÃticos ResistÃncia Cura do plasmÃdio Antibiotics Plasmids Aquatic environment ENGENHARIA DE PESCA |
| dc.subject.eng.fl_str_mv |
Antibiotics Plasmids Aquatic environment |
| dc.subject.cnpq.fl_str_mv |
ENGENHARIA DE PESCA |
| dc.description.abstract.por.fl_txt_mv |
This research work was designed to detect the presence of Aeromonas in the Cocà River estuary, Fortaleza, Cearà State, Brazil. The database consisted of 30 samples of the riverâs surface water and 30 samples of the riverâs sooil, in the period from September, 2007 to April, 2008.. They were amenable, simultaneously, to counting of bacteria on Agar Gelatin Phosphate Salt (GSP) plus 20μg/mL of ampicilim (UFC/mL or UFC/g) The results showed dissemination of Aeromonas in the estuary. The counts for the water samples varied from 10 to 7,050 and from 25 to 38,500 UFC/mL at points A and B respectively; and from 100 to 37,500 UFC/g, and 1,200 to 43500 UFC/g at points A and B respectively. At point C, the counts for water and sediment were smaller than 10 UFC per ml or gram in all samples The occurrence of the greatest indices of Aeromonas in April, at the height of the rainy season, and the lowest in Septemer, at the height of the dry season, suggests there to be a probable seasonality of bacteria density in the studied environment. Among the 41 isolated strains were found the species A. caviae, A. sobria, A. trota, A. salmonicida and A. allossacharophyla. All the strains of Aeromonas sp. were found to be sensitive to cloranfenicol and ceftriaxona except for ampicillim, to which they showed 100% resistance.All the stirps showed resistance to two out of the nine tested antibiotics. After the Curingâ technique, the eritromicina resistance seems to be of plasmidian origin. Este projeto teve como objetivo principal a pesquisa de Aeromonas spp. em Ãgua de superfÃcie e sedimento em trÃs pontos distintos ao longo do rio CocÃ, CearÃ. Foram realizadas coletas no perÃodo de outubro de 2007 a abril de 2008 gerando um total de 30 amostras de Ãgua e 30 de sedimento. A quantificaÃÃo da comunidade bacteriana pertencente ao gÃnero Aeromonas foi feita atravÃs de plaqueamento direto sobre Agar Gelatina Fosfato Sal (Agar GSP acrescido de 20μg/mL de ampicilina). Nas amostras de Ãgua, os valores obtidos variaram de 10 a 7.050 UFC/mL e de 25 a 38.500 UFC/mL nos pontos A e B, respectivamente. Nas amostras de sedimento, as contagens variaram de 100 a 37500 UFC/g e 1.200 a 43.500 UFC/g nos pontos A e B, respectivamente. Nas amostras de Ãgua e sedimento do ponto C, os valores foram menores que 10 UFC (por mL ou g) em todas as coletas. As maiores contagens foram verificadas no mÃs de abril, perÃodo de chuva, e as menores no mÃs de setembro, perÃodo de estiagem. Foram feitos isolamentos e apÃs identificaÃÃo as estirpes foram submetidas à teste de antibiograma e à tÃcnica da âcuraâ do plasmÃdio. Dentre as 41 cepas isoladas, foram identificadas as espÃcies A. caviae, A. sobria, A. trota, A. salmonicida e A. allossacharophyla. Todas as cepas se mostraram sensÃveis ao cloranfenicol e ceftriaxona. Todas as estirpes apresentaram resistÃncia a, pelo menos, dois dos nove antibiÃticos testados. ApÃs a tÃcnica de cura, a maior parte da resistÃncia a eritromicina ficou caracterizada como de origem plasmidial.Conclui-se que o estuÃrio do Rio Cocà està contaminado por Aeromonas e que muitas delas apresentam resistÃncias a antibiÃticos denotando um ambiente poluÃdo e de risco para a populaÃÃo que usa suas Ãguas para lazer, pesca ou outra atividade qualquer. |
| description |
This research work was designed to detect the presence of Aeromonas in the Cocà River estuary, Fortaleza, Cearà State, Brazil. The database consisted of 30 samples of the riverâs surface water and 30 samples of the riverâs sooil, in the period from September, 2007 to April, 2008.. They were amenable, simultaneously, to counting of bacteria on Agar Gelatin Phosphate Salt (GSP) plus 20μg/mL of ampicilim (UFC/mL or UFC/g) The results showed dissemination of Aeromonas in the estuary. The counts for the water samples varied from 10 to 7,050 and from 25 to 38,500 UFC/mL at points A and B respectively; and from 100 to 37,500 UFC/g, and 1,200 to 43500 UFC/g at points A and B respectively. At point C, the counts for water and sediment were smaller than 10 UFC per ml or gram in all samples The occurrence of the greatest indices of Aeromonas in April, at the height of the rainy season, and the lowest in Septemer, at the height of the dry season, suggests there to be a probable seasonality of bacteria density in the studied environment. Among the 41 isolated strains were found the species A. caviae, A. sobria, A. trota, A. salmonicida and A. allossacharophyla. All the strains of Aeromonas sp. were found to be sensitive to cloranfenicol and ceftriaxona except for ampicillim, to which they showed 100% resistance.All the stirps showed resistance to two out of the nine tested antibiotics. After the Curingâ technique, the eritromicina resistance seems to be of plasmidian origin. |
| publishDate |
2009 |
| dc.date.issued.fl_str_mv |
2009-04-24 |
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info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
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masterThesis |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13386 |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13386 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal do Cearà |
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Programa de PÃs-GraduaÃÃo em Engenharia de Pesca |
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UFC |
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BR |
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Universidade Federal do Cearà |
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