Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ

Detalhes bibliográficos
Autor(a) principal: Melissa Soares Medeiros
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=39
Resumo: Introduction: Genotypic testing for HIV-1 drug resistance is useful for selecting antiretroviral drugs for patients developing treatment failure. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. The optimal understanding of its interpretation will give an important tool for HIV treatment. Objective: To identify common combinations of resistance mutations and antiretroviral resistance profile. Methods: Between April 2002 and March 2004, 101 protease and reverse transcriptase (RT) sequences were determined for HIV-1 isolates from patients who were failing antiretroviral therapy. Resistance profile was obtained by Stanford program. Results: male were 76.2%, median age 38 years, CD4 media was 279.21 cells/mm3 and Viral load 4.49 log. Total of 31 mutational patterns were detected to protease inhibitor (IP), 49 to nucleoside RT inhibitor (NRTI), and 17 to nonnucleoside RT inhibitor (NNRTI). K65R was detected in 5.9% isolates. The most frequent mutations were L90M, M184V and K103N to IP, NRTI and NNRTI respectively. The main mutational patterns accounted for 49% of mutant sequences to IP, 38.5% to ITRN accounted and 40,9% to NNRTI. Patients with three or more therapeutic failure had worst resistance profile to all IP except for Lopinavir, and NRTI except for Tenofovir. High resistance to Lamivudine and NNRTI were independent of failure quantity. Conclusion: The best susceptibility was found to Lopinavir at IPâs class and to Tenofovir at ITRNâs. The main mutational patterns to IP, ITRN and NNRTI represented almost half of all patterns found.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisGenotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃGenotipagem e perfil de resistÃncia aos antiretrovirais do virus da imunodeficiÃncia tipo 1 em populaÃÃo com falha terapeutica no CearÃ, Brasil - 2002 a 20042006-02-03Aldo Ãngelo Moreira Lima09055339334http://lattes.cnpq.br/2153321168945169Carlos MaurÃcio de Castro Costa01356810306http://lattes.cnpq.br/9291210203141568 Terezinha do Menino Jesus Silva LeitÃo23484993391http://lattes.cnpq.br/134161063703905574743945372http://lattes.cnpq.br/7680201310065966Melissa Soares MedeirosUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em FarmacologiaUFCBR Inibidores de Protease Inibidores de Transcriptase Reversa Transcriptase Reversa do HIV-1Genotypic testing Mutation HIV-1 Protease Genes Reverse Transcriptase Genes Antiretrovirals HIV-1 resistanceFARMACOLOGIAIntroduction: Genotypic testing for HIV-1 drug resistance is useful for selecting antiretroviral drugs for patients developing treatment failure. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. The optimal understanding of its interpretation will give an important tool for HIV treatment. Objective: To identify common combinations of resistance mutations and antiretroviral resistance profile. Methods: Between April 2002 and March 2004, 101 protease and reverse transcriptase (RT) sequences were determined for HIV-1 isolates from patients who were failing antiretroviral therapy. Resistance profile was obtained by Stanford program. Results: male were 76.2%, median age 38 years, CD4 media was 279.21 cells/mm3 and Viral load 4.49 log. Total of 31 mutational patterns were detected to protease inhibitor (IP), 49 to nucleoside RT inhibitor (NRTI), and 17 to nonnucleoside RT inhibitor (NNRTI). K65R was detected in 5.9% isolates. The most frequent mutations were L90M, M184V and K103N to IP, NRTI and NNRTI respectively. The main mutational patterns accounted for 49% of mutant sequences to IP, 38.5% to ITRN accounted and 40,9% to NNRTI. Patients with three or more therapeutic failure had worst resistance profile to all IP except for Lopinavir, and NRTI except for Tenofovir. High resistance to Lamivudine and NNRTI were independent of failure quantity. Conclusion: The best susceptibility was found to Lopinavir at IPâs class and to Tenofovir at ITRNâs. The main mutational patterns to IP, ITRN and NNRTI represented almost half of all patterns found.A Genotipagem està sendo usada como mÃtodo para guiar a seleÃÃo de antiretrovirais em pacientes com falha terapÃutica. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. Objetivo: Avaliar o perfil de resistÃncia aos antiretrovirais e identificar padrÃes mutacionais das seqÃÃncias de protease e TR do HIV-1. MÃtodos: Foram estudadas as sequÃncias de genes da protease e TR isoladas de 101 amostras de pacientes com HIV-1 em falha terapÃutica, entre abril/2002 a marÃo/2004, atravÃs de Genotipagem realizadas no CearÃ. O Banco de dados de Stanford foi utilizado para avaliaÃÃo de resistÃncia e SPSS versÃo 11 e Epi Info versÃo 6 para anÃlise estatÃstica. Resultados: Sexo masculino 76,2%, mediana de idade 38 anos, CD4 mÃdio de 279,21 cells/mm3 e Carga Viral 4.49 log. Na classe de Inibidores de Protease (IP) 31 padrÃes mutacionais foram encontrados, nos inibidores da transcriptase reversa anÃlogos de nucleosÃdeos (ITRN) 49 e para inibidores da transcriptase reversa nÃo anÃlogos de nucleosÃdeos (ITRNN) 17. As mutaÃÃes mais frequentes foram L90M, M184V e K103N para IP, ITRN e ITRNN espectivamente. A K65R foi detectada em 5,9% dos isolados. TrÃs ou mais falhas terapÃuticas apresentaram maior perfil de resistÃncia para todos os IPs exceto para Lopinavir, e para todos os ITRNs exceto para Tenofovir. Os seis principais padrÃes mutacionais para IPs equivaleram a 49% das sequÃncias, para ITRNs a 38,5%, e para ITRNNs os dois principais padrÃes corresponderam a 40,9%. Foram encontrados altos Ãndices de resistÃncia para ITRNNs independente da quantidade de falhas terapÃuticas. ConclusÃo: Nos IPs a menor resistÃncia encontrada foi ao Lopinavir e nos ITRNs ao Tenofovir. Os principais padrÃes mutacionais para IPs, ITRNs e ITRNNs representaram quase metade de todos os padrÃes de resistÃncia encontrados.Faculdade Christushttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=39application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:13:06Zmail@mail.com -
dc.title.en.fl_str_mv Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
dc.title.alternative.pt.fl_str_mv Genotipagem e perfil de resistÃncia aos antiretrovirais do virus da imunodeficiÃncia tipo 1 em populaÃÃo com falha terapeutica no CearÃ, Brasil - 2002 a 2004
title Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
spellingShingle Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
Melissa Soares Medeiros
Inibidores de Protease
Inibidores de Transcriptase Reversa
Transcriptase Reversa do HIV-1
Genotypic testing
Mutation
HIV-1
Protease Genes
Reverse Transcriptase Genes
Antiretrovirals
HIV-1 resistance
FARMACOLOGIA
title_short Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
title_full Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
title_fullStr Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
title_full_unstemmed Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
title_sort Genotyping and antiretroviral resistance profile test from HIV-1 samples in patients with therapeutic failure from CearÃ
author Melissa Soares Medeiros
author_facet Melissa Soares Medeiros
author_role author
dc.contributor.advisor1.fl_str_mv Aldo Ãngelo Moreira Lima
dc.contributor.advisor1ID.fl_str_mv 09055339334
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2153321168945169
dc.contributor.referee1.fl_str_mv Carlos MaurÃcio de Castro Costa
dc.contributor.referee1ID.fl_str_mv 01356810306
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9291210203141568
dc.contributor.referee2.fl_str_mv Terezinha do Menino Jesus Silva LeitÃo
dc.contributor.referee2ID.fl_str_mv 23484993391
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1341610637039055
dc.contributor.authorID.fl_str_mv 74743945372
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7680201310065966
dc.contributor.author.fl_str_mv Melissa Soares Medeiros
contributor_str_mv Aldo Ãngelo Moreira Lima
Carlos MaurÃcio de Castro Costa
Terezinha do Menino Jesus Silva LeitÃo
dc.subject.por.fl_str_mv Inibidores de Protease
Inibidores de Transcriptase Reversa
Transcriptase Reversa do HIV-1
topic Inibidores de Protease
Inibidores de Transcriptase Reversa
Transcriptase Reversa do HIV-1
Genotypic testing
Mutation
HIV-1
Protease Genes
Reverse Transcriptase Genes
Antiretrovirals
HIV-1 resistance
FARMACOLOGIA
dc.subject.eng.fl_str_mv Genotypic testing
Mutation
HIV-1
Protease Genes
Reverse Transcriptase Genes
Antiretrovirals
HIV-1 resistance
dc.subject.cnpq.fl_str_mv FARMACOLOGIA
dc.description.sponsorship.fl_txt_mv Faculdade Christus
dc.description.abstract.por.fl_txt_mv Introduction: Genotypic testing for HIV-1 drug resistance is useful for selecting antiretroviral drugs for patients developing treatment failure. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. The optimal understanding of its interpretation will give an important tool for HIV treatment. Objective: To identify common combinations of resistance mutations and antiretroviral resistance profile. Methods: Between April 2002 and March 2004, 101 protease and reverse transcriptase (RT) sequences were determined for HIV-1 isolates from patients who were failing antiretroviral therapy. Resistance profile was obtained by Stanford program. Results: male were 76.2%, median age 38 years, CD4 media was 279.21 cells/mm3 and Viral load 4.49 log. Total of 31 mutational patterns were detected to protease inhibitor (IP), 49 to nucleoside RT inhibitor (NRTI), and 17 to nonnucleoside RT inhibitor (NNRTI). K65R was detected in 5.9% isolates. The most frequent mutations were L90M, M184V and K103N to IP, NRTI and NNRTI respectively. The main mutational patterns accounted for 49% of mutant sequences to IP, 38.5% to ITRN accounted and 40,9% to NNRTI. Patients with three or more therapeutic failure had worst resistance profile to all IP except for Lopinavir, and NRTI except for Tenofovir. High resistance to Lamivudine and NNRTI were independent of failure quantity. Conclusion: The best susceptibility was found to Lopinavir at IPâs class and to Tenofovir at ITRNâs. The main mutational patterns to IP, ITRN and NNRTI represented almost half of all patterns found.
A Genotipagem està sendo usada como mÃtodo para guiar a seleÃÃo de antiretrovirais em pacientes com falha terapÃutica. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. Objetivo: Avaliar o perfil de resistÃncia aos antiretrovirais e identificar padrÃes mutacionais das seqÃÃncias de protease e TR do HIV-1. MÃtodos: Foram estudadas as sequÃncias de genes da protease e TR isoladas de 101 amostras de pacientes com HIV-1 em falha terapÃutica, entre abril/2002 a marÃo/2004, atravÃs de Genotipagem realizadas no CearÃ. O Banco de dados de Stanford foi utilizado para avaliaÃÃo de resistÃncia e SPSS versÃo 11 e Epi Info versÃo 6 para anÃlise estatÃstica. Resultados: Sexo masculino 76,2%, mediana de idade 38 anos, CD4 mÃdio de 279,21 cells/mm3 e Carga Viral 4.49 log. Na classe de Inibidores de Protease (IP) 31 padrÃes mutacionais foram encontrados, nos inibidores da transcriptase reversa anÃlogos de nucleosÃdeos (ITRN) 49 e para inibidores da transcriptase reversa nÃo anÃlogos de nucleosÃdeos (ITRNN) 17. As mutaÃÃes mais frequentes foram L90M, M184V e K103N para IP, ITRN e ITRNN espectivamente. A K65R foi detectada em 5,9% dos isolados. TrÃs ou mais falhas terapÃuticas apresentaram maior perfil de resistÃncia para todos os IPs exceto para Lopinavir, e para todos os ITRNs exceto para Tenofovir. Os seis principais padrÃes mutacionais para IPs equivaleram a 49% das sequÃncias, para ITRNs a 38,5%, e para ITRNNs os dois principais padrÃes corresponderam a 40,9%. Foram encontrados altos Ãndices de resistÃncia para ITRNNs independente da quantidade de falhas terapÃuticas. ConclusÃo: Nos IPs a menor resistÃncia encontrada foi ao Lopinavir e nos ITRNs ao Tenofovir. Os principais padrÃes mutacionais para IPs, ITRNs e ITRNNs representaram quase metade de todos os padrÃes de resistÃncia encontrados.
description Introduction: Genotypic testing for HIV-1 drug resistance is useful for selecting antiretroviral drugs for patients developing treatment failure. O melhor entendimento da sua interpretaÃÃo facilitarà sua utilizaÃÃo como ferramenta mÃdica na terapÃutica do HIV. The optimal understanding of its interpretation will give an important tool for HIV treatment. Objective: To identify common combinations of resistance mutations and antiretroviral resistance profile. Methods: Between April 2002 and March 2004, 101 protease and reverse transcriptase (RT) sequences were determined for HIV-1 isolates from patients who were failing antiretroviral therapy. Resistance profile was obtained by Stanford program. Results: male were 76.2%, median age 38 years, CD4 media was 279.21 cells/mm3 and Viral load 4.49 log. Total of 31 mutational patterns were detected to protease inhibitor (IP), 49 to nucleoside RT inhibitor (NRTI), and 17 to nonnucleoside RT inhibitor (NNRTI). K65R was detected in 5.9% isolates. The most frequent mutations were L90M, M184V and K103N to IP, NRTI and NNRTI respectively. The main mutational patterns accounted for 49% of mutant sequences to IP, 38.5% to ITRN accounted and 40,9% to NNRTI. Patients with three or more therapeutic failure had worst resistance profile to all IP except for Lopinavir, and NRTI except for Tenofovir. High resistance to Lamivudine and NNRTI were independent of failure quantity. Conclusion: The best susceptibility was found to Lopinavir at IPâs class and to Tenofovir at ITRNâs. The main mutational patterns to IP, ITRN and NNRTI represented almost half of all patterns found.
publishDate 2006
dc.date.issued.fl_str_mv 2006-02-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=39
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Farmacologia
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
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instname_str Universidade Federal do Ceará
instacron_str UFC
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