Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce

Detalhes bibliográficos
Autor(a) principal: Maria Nataniela da Silva
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17280
Resumo: Natural and synthetic estrogens are naturally excreted by humans and some vertebrates. These emerging contaminants classified as endocrine disruptors (EDs) present, even in small quantities, in water and sediment are able to affect the endocrine system and the stages of growth and reproduction of aquatic organisms and humans. In this context, this study aimed to perform a physical-chemical and microbiological characterization of reservoir water from Santo AnastÃcio, located in CearÃ, Brazil, and the develop and validate an analytical method using High-performance liquid chromatography with detector diode array (HPLC-DAD) for the determination of caffeine (CAF) and estrone hormones, 17β-estradiol, estriol (natural) and 17α-ethinylestradiol (synthetic) for water and sediment phase of reservoir water studied. The physicochemical parameters evaluated were phosphorus, chlorophyll A, pheophytin, nitrogen, chloride, sulfide, hardness, calcium, magnesium, pH, alkalinity, turbidity and total and dissolved solids. Microbiological characterization evaluated the presence of total and fecal coliforms. For the determination of EDs by HPLC-DAD was used to extract solid phase techniques (SPE) for water sample and QuEChERS for the sediment sample. The results of physicochemical analyzes indicated above the allowed values by CONAMA 357/05 for class 2, only to phosphorus and sulfide. Microbiological characterization indicated the presence of coliforms far above the values stipulated by CONAMA. In determining the DEs concentrations from 0.54 to 0.93 μg L-1 were detected caffeine in water, and 0.76 to 4.94 mg kg-1 17β estradiol and estriol in the sediment. Therefore, the water reservoir is unsuitable for direct consumption and recreation within the law. The validated analytical method was efficient for the determination of analytes can be used for routine analysis since there is no legislation to caffeine and EDs.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDetermination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/CeDeterminaÃÃo de desreguladores endocrinos na Ãgua e no sedimentos do aÃude Santo AnastÃcio na cidade de Fortaleza/Ce2016-02-24Helena Becker11756179387http://lattes.cnpq.br/4283472486715232Ronaldo Ferreira do Nascimento42824397349 http://lattes.cnpq.br/6345440666273561Francisco Wagner de Sousa6156506730451157799353http://lattes.cnpq.br/9264050084665519Maria Nataniela da SilvaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em QuÃmica UFCBRDesreguladores endÃcrinos AÃude Santo AnastÃcio SPE QuEChERS HPLC â DAD Endocrine Disruptors Weir Santo Anastacio SPE QuEChERS HPLC - DADQUIMICA ANALITICAQUIMICA ANALITICANatural and synthetic estrogens are naturally excreted by humans and some vertebrates. These emerging contaminants classified as endocrine disruptors (EDs) present, even in small quantities, in water and sediment are able to affect the endocrine system and the stages of growth and reproduction of aquatic organisms and humans. In this context, this study aimed to perform a physical-chemical and microbiological characterization of reservoir water from Santo AnastÃcio, located in CearÃ, Brazil, and the develop and validate an analytical method using High-performance liquid chromatography with detector diode array (HPLC-DAD) for the determination of caffeine (CAF) and estrone hormones, 17β-estradiol, estriol (natural) and 17α-ethinylestradiol (synthetic) for water and sediment phase of reservoir water studied. The physicochemical parameters evaluated were phosphorus, chlorophyll A, pheophytin, nitrogen, chloride, sulfide, hardness, calcium, magnesium, pH, alkalinity, turbidity and total and dissolved solids. Microbiological characterization evaluated the presence of total and fecal coliforms. For the determination of EDs by HPLC-DAD was used to extract solid phase techniques (SPE) for water sample and QuEChERS for the sediment sample. The results of physicochemical analyzes indicated above the allowed values by CONAMA 357/05 for class 2, only to phosphorus and sulfide. Microbiological characterization indicated the presence of coliforms far above the values stipulated by CONAMA. In determining the DEs concentrations from 0.54 to 0.93 μg L-1 were detected caffeine in water, and 0.76 to 4.94 mg kg-1 17β estradiol and estriol in the sediment. Therefore, the water reservoir is unsuitable for direct consumption and recreation within the law. The validated analytical method was efficient for the determination of analytes can be used for routine analysis since there is no legislation to caffeine and EDs.EstrÃgenos naturais e sintÃticos sÃo naturalmente excretados por seres humanos e alguns vertebrados. Estes contaminantes emergentes classificados como desreguladores endÃcrinos (DEs) presentes, mesmo em pequenas quantidades, na Ãgua e no sedimento sÃo capazes de afetar o sistema endÃcrino e as fases de crescimento e reproduÃÃo de organismos aquÃticos e humanos. Nesse contexto, este trabalho teve como objetivos efetuar uma caracterizaÃÃo fÃsico-quÃmica e microbiolÃgica da Ãgua do aÃude Santo AnastÃcio, localizado no CearÃ, Brazil, alÃm de desenvolver e validar um mÃtodo analÃtico empregando a cromatografia lÃquida de alta eficiÃncia com detector de arranjos de diodos (HPLC-DAD) para determinaÃÃo da cafeÃna e dos hormÃnios estrona, 17β-estradiol, estriol (naturais) e 17α-etinilestradiol (sintÃtico) na Ãgua e no sedimento do aÃude mencionado. Os parÃmetros fÃsico-quÃmicos avaliados foram fÃsforo, clorofila A, feofitina, nitrogÃnio, cloreto, sulfeto, dureza, cÃlcio, magnÃsio, pH, alcalinidade, turbidez e sÃlidos totais e dissolvidos. A caracterizaÃÃo microbiolÃgica avaliou a presenÃa de coliformes totais e termotolerantes. Para a determinaÃÃo dos DEs por HPLC-DAD utilizou-se as tÃcnicas de extraÃÃo em fase sÃlida (SPE) para a amostra de Ãgua e QuEChERS para a amostra de sedimento. Os resultados das anÃlises fÃsico-quÃmicas indicaram valores acima do permitido pelo CONAMA 357/05, para classe 2, apenas para fÃsforo e sulfeto. A caracterizaÃÃo microbiolÃgica indicou a presenÃa de coliformes muito acima dos valores estipulados pelo CONAMA. Na determinaÃÃo dos DEs foram detectados as concentraÃÃes de 0,54 a 0,93 μg L-1 de cafeÃna na Ãgua, e, 0,76 a 4,94 mg kg-1 do 17β estradiol e estriol no sedimento. Diante disso, a Ãgua do aÃude encontra-se inadequada para consumo direto e recreaÃÃo dentro da legislaÃÃo vigente. O mÃtodo analÃtico validado mostrou-se eficiente para determinaÃÃo dos analitos podendo ser utilizado para anÃlises de rotina uma vez que nÃo existe legislaÃÃo para a cafeÃna e os DEs.CoordenaÃÃo de AperfeiÃoamento de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17280application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:30:39Zmail@mail.com -
dc.title.en.fl_str_mv Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
dc.title.alternative.pt.fl_str_mv DeterminaÃÃo de desreguladores endocrinos na Ãgua e no sedimentos do aÃude Santo AnastÃcio na cidade de Fortaleza/Ce
title Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
spellingShingle Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
Maria Nataniela da Silva
Desreguladores endÃcrinos
AÃude Santo AnastÃcio
SPE
QuEChERS
HPLC â DAD
Endocrine Disruptors
Weir Santo Anastacio
SPE
QuEChERS
HPLC - DAD
QUIMICA ANALITICA
QUIMICA ANALITICA
title_short Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
title_full Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
title_fullStr Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
title_full_unstemmed Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
title_sort Determination of endocrine disruptors in water and sediment from Santo AnastÃcio weir in the city Fortaleza/Ce
author Maria Nataniela da Silva
author_facet Maria Nataniela da Silva
author_role author
dc.contributor.advisor1.fl_str_mv Helena Becker
dc.contributor.advisor1ID.fl_str_mv 11756179387
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4283472486715232
dc.contributor.referee1.fl_str_mv Ronaldo Ferreira do Nascimento
dc.contributor.referee1ID.fl_str_mv 42824397349
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6345440666273561
dc.contributor.referee2.fl_str_mv Francisco Wagner de Sousa
dc.contributor.referee2ID.fl_str_mv 61565067304
dc.contributor.authorID.fl_str_mv 51157799353
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9264050084665519
dc.contributor.author.fl_str_mv Maria Nataniela da Silva
contributor_str_mv Helena Becker
Ronaldo Ferreira do Nascimento
Francisco Wagner de Sousa
dc.subject.por.fl_str_mv Desreguladores endÃcrinos
AÃude Santo AnastÃcio
SPE
QuEChERS
HPLC â DAD
topic Desreguladores endÃcrinos
AÃude Santo AnastÃcio
SPE
QuEChERS
HPLC â DAD
Endocrine Disruptors
Weir Santo Anastacio
SPE
QuEChERS
HPLC - DAD
QUIMICA ANALITICA
QUIMICA ANALITICA
dc.subject.eng.fl_str_mv Endocrine Disruptors
Weir Santo Anastacio
SPE
QuEChERS
HPLC - DAD
dc.subject.cnpq.fl_str_mv QUIMICA ANALITICA
QUIMICA ANALITICA
dc.description.sponsorship.fl_txt_mv CoordenaÃÃo de AperfeiÃoamento de NÃvel Superior
dc.description.abstract.por.fl_txt_mv Natural and synthetic estrogens are naturally excreted by humans and some vertebrates. These emerging contaminants classified as endocrine disruptors (EDs) present, even in small quantities, in water and sediment are able to affect the endocrine system and the stages of growth and reproduction of aquatic organisms and humans. In this context, this study aimed to perform a physical-chemical and microbiological characterization of reservoir water from Santo AnastÃcio, located in CearÃ, Brazil, and the develop and validate an analytical method using High-performance liquid chromatography with detector diode array (HPLC-DAD) for the determination of caffeine (CAF) and estrone hormones, 17β-estradiol, estriol (natural) and 17α-ethinylestradiol (synthetic) for water and sediment phase of reservoir water studied. The physicochemical parameters evaluated were phosphorus, chlorophyll A, pheophytin, nitrogen, chloride, sulfide, hardness, calcium, magnesium, pH, alkalinity, turbidity and total and dissolved solids. Microbiological characterization evaluated the presence of total and fecal coliforms. For the determination of EDs by HPLC-DAD was used to extract solid phase techniques (SPE) for water sample and QuEChERS for the sediment sample. The results of physicochemical analyzes indicated above the allowed values by CONAMA 357/05 for class 2, only to phosphorus and sulfide. Microbiological characterization indicated the presence of coliforms far above the values stipulated by CONAMA. In determining the DEs concentrations from 0.54 to 0.93 μg L-1 were detected caffeine in water, and 0.76 to 4.94 mg kg-1 17β estradiol and estriol in the sediment. Therefore, the water reservoir is unsuitable for direct consumption and recreation within the law. The validated analytical method was efficient for the determination of analytes can be used for routine analysis since there is no legislation to caffeine and EDs.
EstrÃgenos naturais e sintÃticos sÃo naturalmente excretados por seres humanos e alguns vertebrados. Estes contaminantes emergentes classificados como desreguladores endÃcrinos (DEs) presentes, mesmo em pequenas quantidades, na Ãgua e no sedimento sÃo capazes de afetar o sistema endÃcrino e as fases de crescimento e reproduÃÃo de organismos aquÃticos e humanos. Nesse contexto, este trabalho teve como objetivos efetuar uma caracterizaÃÃo fÃsico-quÃmica e microbiolÃgica da Ãgua do aÃude Santo AnastÃcio, localizado no CearÃ, Brazil, alÃm de desenvolver e validar um mÃtodo analÃtico empregando a cromatografia lÃquida de alta eficiÃncia com detector de arranjos de diodos (HPLC-DAD) para determinaÃÃo da cafeÃna e dos hormÃnios estrona, 17β-estradiol, estriol (naturais) e 17α-etinilestradiol (sintÃtico) na Ãgua e no sedimento do aÃude mencionado. Os parÃmetros fÃsico-quÃmicos avaliados foram fÃsforo, clorofila A, feofitina, nitrogÃnio, cloreto, sulfeto, dureza, cÃlcio, magnÃsio, pH, alcalinidade, turbidez e sÃlidos totais e dissolvidos. A caracterizaÃÃo microbiolÃgica avaliou a presenÃa de coliformes totais e termotolerantes. Para a determinaÃÃo dos DEs por HPLC-DAD utilizou-se as tÃcnicas de extraÃÃo em fase sÃlida (SPE) para a amostra de Ãgua e QuEChERS para a amostra de sedimento. Os resultados das anÃlises fÃsico-quÃmicas indicaram valores acima do permitido pelo CONAMA 357/05, para classe 2, apenas para fÃsforo e sulfeto. A caracterizaÃÃo microbiolÃgica indicou a presenÃa de coliformes muito acima dos valores estipulados pelo CONAMA. Na determinaÃÃo dos DEs foram detectados as concentraÃÃes de 0,54 a 0,93 μg L-1 de cafeÃna na Ãgua, e, 0,76 a 4,94 mg kg-1 do 17β estradiol e estriol no sedimento. Diante disso, a Ãgua do aÃude encontra-se inadequada para consumo direto e recreaÃÃo dentro da legislaÃÃo vigente. O mÃtodo analÃtico validado mostrou-se eficiente para determinaÃÃo dos analitos podendo ser utilizado para anÃlises de rotina uma vez que nÃo existe legislaÃÃo para a cafeÃna e os DEs.
description Natural and synthetic estrogens are naturally excreted by humans and some vertebrates. These emerging contaminants classified as endocrine disruptors (EDs) present, even in small quantities, in water and sediment are able to affect the endocrine system and the stages of growth and reproduction of aquatic organisms and humans. In this context, this study aimed to perform a physical-chemical and microbiological characterization of reservoir water from Santo AnastÃcio, located in CearÃ, Brazil, and the develop and validate an analytical method using High-performance liquid chromatography with detector diode array (HPLC-DAD) for the determination of caffeine (CAF) and estrone hormones, 17β-estradiol, estriol (natural) and 17α-ethinylestradiol (synthetic) for water and sediment phase of reservoir water studied. The physicochemical parameters evaluated were phosphorus, chlorophyll A, pheophytin, nitrogen, chloride, sulfide, hardness, calcium, magnesium, pH, alkalinity, turbidity and total and dissolved solids. Microbiological characterization evaluated the presence of total and fecal coliforms. For the determination of EDs by HPLC-DAD was used to extract solid phase techniques (SPE) for water sample and QuEChERS for the sediment sample. The results of physicochemical analyzes indicated above the allowed values by CONAMA 357/05 for class 2, only to phosphorus and sulfide. Microbiological characterization indicated the presence of coliforms far above the values stipulated by CONAMA. In determining the DEs concentrations from 0.54 to 0.93 μg L-1 were detected caffeine in water, and 0.76 to 4.94 mg kg-1 17β estradiol and estriol in the sediment. Therefore, the water reservoir is unsuitable for direct consumption and recreation within the law. The validated analytical method was efficient for the determination of analytes can be used for routine analysis since there is no legislation to caffeine and EDs.
publishDate 2016
dc.date.issued.fl_str_mv 2016-02-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17280
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em QuÃmica
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
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reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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