Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ

Detalhes bibliográficos
Autor(a) principal: Maria NÃgila Carneiro Matos
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11219
Resumo: Proteomic studies of sperm cells may be contribute in the biological reproduction potential by mapping biomarkers of fertility. The present study aims to evaluate the effect of seasonality in the protein profile of sperm Moxotà breed goats, in order to identify markers of fertility. We used six animals in reproductive age (24-36 months) for the collection of semen in artificial vagina, which was carried out weekly during the dry and rainy seasons. After collection, semen was submitted to evaluation of sperm parameters (motility, vigor, concentration and testis volume). The profile of sperm proteins was obtained using the two-dimensional electrophoresis technique . Analysis of seminal parameters showed no significant differences between periods. We detected 330 and 246 spots for 2D gels representing the rainy and dry seasons. The distribution of spots through isoelectric point in the two study periods are concentrated in the pH range 5-6, both in the dry season (PS) and in the rainy season (PC) this region contains about 50% of the total protein . Regarding the molecular weight of 20-40 kDa proteins showed greater frequency over the years. It was observed the presence of 56 spots similar and constant expression in six animals. The qualitative and quantitative differences were observed between the season, highlighting the 349 spot that was present only in the PC, while the 566 and 1115 spots were differentially expressed in the dry season. Individual differences have been identified yet and it seems is correlated with sperm parameters evaluated. In general there was greater expression of proteins in the PS. In conclusion, the results show the influence of seasonality on the expression of some proteins, which may be strong candidates for biomarkers of fertility and may be related to some characteristics such as sperm maturation, capacitation, acrosome reaction and interaction between the gametes.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEfeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃSeasonality effect in protein profile of sperm Moxotà goats2012-02-28Celso Shiniti Nagano81850786100http://lattes.cnpq.br/7732407650135099JoÃo Batista Cajazeiras478 389 303 91http://lattes.cnpq.br/1947326193452969 Rodrigo Maranguape Silva da Cunha70290008387http://lattes.cnpq.br/191805606794342902143589336http://lattes.cnpq.br/1294869185940983Maria NÃgila Carneiro MatosUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Biotecnologia (Campus da UFC em Sobral-CE)UFCBRProteomics Spermatozoa Biomarkers Goats Moxotà breedProteÃmica EspermatozÃides Biomarcadores Caprino RaÃa MoxotÃREPRODUCAO ANIMALProteomic studies of sperm cells may be contribute in the biological reproduction potential by mapping biomarkers of fertility. The present study aims to evaluate the effect of seasonality in the protein profile of sperm Moxotà breed goats, in order to identify markers of fertility. We used six animals in reproductive age (24-36 months) for the collection of semen in artificial vagina, which was carried out weekly during the dry and rainy seasons. After collection, semen was submitted to evaluation of sperm parameters (motility, vigor, concentration and testis volume). The profile of sperm proteins was obtained using the two-dimensional electrophoresis technique . Analysis of seminal parameters showed no significant differences between periods. We detected 330 and 246 spots for 2D gels representing the rainy and dry seasons. The distribution of spots through isoelectric point in the two study periods are concentrated in the pH range 5-6, both in the dry season (PS) and in the rainy season (PC) this region contains about 50% of the total protein . Regarding the molecular weight of 20-40 kDa proteins showed greater frequency over the years. It was observed the presence of 56 spots similar and constant expression in six animals. The qualitative and quantitative differences were observed between the season, highlighting the 349 spot that was present only in the PC, while the 566 and 1115 spots were differentially expressed in the dry season. Individual differences have been identified yet and it seems is correlated with sperm parameters evaluated. In general there was greater expression of proteins in the PS. In conclusion, the results show the influence of seasonality on the expression of some proteins, which may be strong candidates for biomarkers of fertility and may be related to some characteristics such as sperm maturation, capacitation, acrosome reaction and interaction between the gametes.Estudos proteÃmicos da cÃlula espermÃtica podem contribuir para a utilizaÃÃo do potencial biolÃgico reprodutivo atravÃs do mapeamento de biomarcadores de fertilidade. O presente trabalho tem o objetivo de avaliar o efeito da sazonalidade no perfil proteico de espermatozÃides da raÃa caprina MoxotÃ, visando à identificaÃÃo de marcadores de fertilidade. Foram utilizados seis reprodutores em idade reprodutiva (24 a 36 meses) para a coleta de sÃmen em vaginal artificial, a qual foi realizada semanalmente durante os perÃodos seco e chuvoso. ApÃs a coleta, o sÃmen foi submetido à avaliaÃÃo dos parÃmetros espermÃticos (motilidade, vigor, concentraÃÃo e volume espermÃtico). O perfil das proteÃnas espermÃticas foi obtido atravÃs da tÃcnica de eletroforese bidimensional (2D). A anÃlise dos parÃmetros seminais nÃo apresentou diferenÃas significativas entre os perÃodos. Foram detectados 330 e 246 spots em gÃis 2D para os perÃodos chuvoso e seco, respectivamente. A distribuiÃÃo dos spots pelo ponto isoelÃtrico nos dois perÃodos estudados, encontram-se majoritariamente concentrados na faixa de pH de 5 a 6, tanto no perÃodo seco (PS) quanto no perÃodo chuvoso (PC) essa regiÃo contem cerca 50% do total de proteÃnas. Em relaÃÃo a massa molecular as proteÃnas de 20-40 kDa apresentaram maior frequÃncia ao longo do ano. Observou-se a presenÃa de 56 spots similares e com expressÃo constante nos seis animais. As diferenÃas qualitativas e quantitativas foram observadas entre os perÃodos, destacando-se o spot 349 que esteve presente apenas no PC, enquanto que os spots 566 e 1115 foram diferencialmente expressos no PS. Foram identificadas ainda diferenÃas individuais e estas parecem està correlacionadas com os parÃmetros espermÃticos avaliados. De uma forma geral observou-se maior expressÃo de proteÃnas no PC. Em conclusÃo, os resultados apontam influÃncia da sazonalidade na expressÃo de algumas proteÃnas, as quais podem ser fortes candidatas a biomarcadores de fertilidade e podem estar relacionadas a vÃrias caracterÃsticas do espermatozÃide como maturaÃÃo, capacitaÃÃo, reaÃÃo acrossÃmica e interaÃÃo entre os gametas.CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11219application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:24:28Zmail@mail.com -
dc.title.pt.fl_str_mv Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
dc.title.alternative..fl_str_mv Seasonality effect in protein profile of sperm Moxotà goats
title Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
spellingShingle Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
Maria NÃgila Carneiro Matos
ProteÃmica
EspermatozÃides
Biomarcadores
Caprino
RaÃa MoxotÃ
REPRODUCAO ANIMAL
title_short Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
title_full Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
title_fullStr Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
title_full_unstemmed Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
title_sort Efeito da sazonalidade no perfil de proteÃnas de espermatozÃides de caprinos da raÃa MoxotÃ
author Maria NÃgila Carneiro Matos
author_facet Maria NÃgila Carneiro Matos
author_role author
dc.contributor.advisor1.fl_str_mv Celso Shiniti Nagano
dc.contributor.advisor1ID.fl_str_mv 81850786100
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7732407650135099
dc.contributor.referee1.fl_str_mv JoÃo Batista Cajazeiras
dc.contributor.referee1ID.fl_str_mv 478 389 303 91
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1947326193452969
dc.contributor.referee2.fl_str_mv Rodrigo Maranguape Silva da Cunha
dc.contributor.referee2ID.fl_str_mv 70290008387
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1918056067943429
dc.contributor.authorID.fl_str_mv 02143589336
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1294869185940983
dc.contributor.author.fl_str_mv Maria NÃgila Carneiro Matos
contributor_str_mv Celso Shiniti Nagano
JoÃo Batista Cajazeiras
Rodrigo Maranguape Silva da Cunha
dc.subject.por.fl_str_mv ProteÃmica
EspermatozÃides
Biomarcadores
Caprino
RaÃa MoxotÃ
topic ProteÃmica
EspermatozÃides
Biomarcadores
Caprino
RaÃa MoxotÃ
REPRODUCAO ANIMAL
dc.subject.cnpq.fl_str_mv REPRODUCAO ANIMAL
dc.description.sponsorship.fl_txt_mv CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
dc.description.abstract..fl_txt_mv Proteomic studies of sperm cells may be contribute in the biological reproduction potential by mapping biomarkers of fertility. The present study aims to evaluate the effect of seasonality in the protein profile of sperm Moxotà breed goats, in order to identify markers of fertility. We used six animals in reproductive age (24-36 months) for the collection of semen in artificial vagina, which was carried out weekly during the dry and rainy seasons. After collection, semen was submitted to evaluation of sperm parameters (motility, vigor, concentration and testis volume). The profile of sperm proteins was obtained using the two-dimensional electrophoresis technique . Analysis of seminal parameters showed no significant differences between periods. We detected 330 and 246 spots for 2D gels representing the rainy and dry seasons. The distribution of spots through isoelectric point in the two study periods are concentrated in the pH range 5-6, both in the dry season (PS) and in the rainy season (PC) this region contains about 50% of the total protein . Regarding the molecular weight of 20-40 kDa proteins showed greater frequency over the years. It was observed the presence of 56 spots similar and constant expression in six animals. The qualitative and quantitative differences were observed between the season, highlighting the 349 spot that was present only in the PC, while the 566 and 1115 spots were differentially expressed in the dry season. Individual differences have been identified yet and it seems is correlated with sperm parameters evaluated. In general there was greater expression of proteins in the PS. In conclusion, the results show the influence of seasonality on the expression of some proteins, which may be strong candidates for biomarkers of fertility and may be related to some characteristics such as sperm maturation, capacitation, acrosome reaction and interaction between the gametes.
dc.description.abstract.por.fl_txt_mv Estudos proteÃmicos da cÃlula espermÃtica podem contribuir para a utilizaÃÃo do potencial biolÃgico reprodutivo atravÃs do mapeamento de biomarcadores de fertilidade. O presente trabalho tem o objetivo de avaliar o efeito da sazonalidade no perfil proteico de espermatozÃides da raÃa caprina MoxotÃ, visando à identificaÃÃo de marcadores de fertilidade. Foram utilizados seis reprodutores em idade reprodutiva (24 a 36 meses) para a coleta de sÃmen em vaginal artificial, a qual foi realizada semanalmente durante os perÃodos seco e chuvoso. ApÃs a coleta, o sÃmen foi submetido à avaliaÃÃo dos parÃmetros espermÃticos (motilidade, vigor, concentraÃÃo e volume espermÃtico). O perfil das proteÃnas espermÃticas foi obtido atravÃs da tÃcnica de eletroforese bidimensional (2D). A anÃlise dos parÃmetros seminais nÃo apresentou diferenÃas significativas entre os perÃodos. Foram detectados 330 e 246 spots em gÃis 2D para os perÃodos chuvoso e seco, respectivamente. A distribuiÃÃo dos spots pelo ponto isoelÃtrico nos dois perÃodos estudados, encontram-se majoritariamente concentrados na faixa de pH de 5 a 6, tanto no perÃodo seco (PS) quanto no perÃodo chuvoso (PC) essa regiÃo contem cerca 50% do total de proteÃnas. Em relaÃÃo a massa molecular as proteÃnas de 20-40 kDa apresentaram maior frequÃncia ao longo do ano. Observou-se a presenÃa de 56 spots similares e com expressÃo constante nos seis animais. As diferenÃas qualitativas e quantitativas foram observadas entre os perÃodos, destacando-se o spot 349 que esteve presente apenas no PC, enquanto que os spots 566 e 1115 foram diferencialmente expressos no PS. Foram identificadas ainda diferenÃas individuais e estas parecem està correlacionadas com os parÃmetros espermÃticos avaliados. De uma forma geral observou-se maior expressÃo de proteÃnas no PC. Em conclusÃo, os resultados apontam influÃncia da sazonalidade na expressÃo de algumas proteÃnas, as quais podem ser fortes candidatas a biomarcadores de fertilidade e podem estar relacionadas a vÃrias caracterÃsticas do espermatozÃide como maturaÃÃo, capacitaÃÃo, reaÃÃo acrossÃmica e interaÃÃo entre os gametas.
description Proteomic studies of sperm cells may be contribute in the biological reproduction potential by mapping biomarkers of fertility. The present study aims to evaluate the effect of seasonality in the protein profile of sperm Moxotà breed goats, in order to identify markers of fertility. We used six animals in reproductive age (24-36 months) for the collection of semen in artificial vagina, which was carried out weekly during the dry and rainy seasons. After collection, semen was submitted to evaluation of sperm parameters (motility, vigor, concentration and testis volume). The profile of sperm proteins was obtained using the two-dimensional electrophoresis technique . Analysis of seminal parameters showed no significant differences between periods. We detected 330 and 246 spots for 2D gels representing the rainy and dry seasons. The distribution of spots through isoelectric point in the two study periods are concentrated in the pH range 5-6, both in the dry season (PS) and in the rainy season (PC) this region contains about 50% of the total protein . Regarding the molecular weight of 20-40 kDa proteins showed greater frequency over the years. It was observed the presence of 56 spots similar and constant expression in six animals. The qualitative and quantitative differences were observed between the season, highlighting the 349 spot that was present only in the PC, while the 566 and 1115 spots were differentially expressed in the dry season. Individual differences have been identified yet and it seems is correlated with sperm parameters evaluated. In general there was greater expression of proteins in the PS. In conclusion, the results show the influence of seasonality on the expression of some proteins, which may be strong candidates for biomarkers of fertility and may be related to some characteristics such as sperm maturation, capacitation, acrosome reaction and interaction between the gametes.
publishDate 2012
dc.date.issued.fl_str_mv 2012-02-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11219
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Biotecnologia (Campus da UFC em Sobral-CE)
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
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reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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