Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15629 |
Resumo: | Soursop (Annona muricata L.) is a tree of Annonaceae family widely distributed in all tropical regions of the world, produces fruit known as graviola and its leaves are used in traditional medicine as phytotherapy. Some compounds present in A. muricata have been related to antioxidant activity, anti-inflammatory and antimicrobial activities of this plant. This study aimed to evaluate the antioxidant, anti-inflammatory and antibacterial activities of hexane (HE) and ethanol (EE) soursop leaves extracts. To this end, we used soursop leaves for preparation of extracts for further analyzes of phytochemical composition, antioxidant capacity, ear edema assays in vivo and in vitro antibacterial activity. For analysis of the antioxidant activity of hexane and ethanol extracts were determined total polyphenols, yellows flavonoids and anthocyanins and total antioxidant capacity by ABTS●+ and DPPH methods. To evaluate the anti-inflammatory activity of the extracts was performed ear edema model induced by xylene or TPA in mice (Swiss females, 25-35g)-treated intragastrically (ig) for 7 consecutive days or topically (single dose) of different concentrations of the extracts (EE or HE):10, 100 and 1000 mg/kg. The antimicrobial activity of the extracts was evaluated against strains of Staphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) and Salmonella choleraesuis (ATCC 14028). The extracts showed wide variety of phytochemical compounds, especially EE, and high antioxidant activity. Pretreatment via i.g. with the extracts was able to inhibit the edema induced by xylene, with a maximum percentage of inhibition of 61.72% for EE 10 and 57.08% for HE 100, but no effect on TPA-induced edema. The topical application with HE inhibited in a dose-dependent form the xylene-induced edema with maximum inhibition of 78.89% for HE1000, while for EE1000 was 67.95%, with no significant difference EE10 and EE100. As for the TPA-induced edema, HE (10, 100 and 1000 mg / kg) inhibited edema at 84.39%, 68.68% and 65.96%, while for EE (10, 100 and 1000 mg / kg ) the inhibition was 75%, 56% and 51%, respectively. Moreover, the extracts showed no antibacterial activity against all tested strains. The hexane and ethanol extracts of soursop leaves exhibit high differentiated antioxidant and anti-inflammatory activity compared to edema induced by xylene and TPA. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAntioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.)Atividades antioxidante, anti-inflamatÃria e antibacteriana dos extratos hexÃnico e etanÃlico das folhas da gravioleira (Annona muricata L.)2013-03-05Dirce Fernandes de Melo02120178372http://lattes.cnpq.br/6669454140368626Ãrika Freitas Mota54454956391http://lattes.cnpq.br/5964165127429630Diana CÃlia Sousa Nunes-Pinheiro15563278387http://lattes.cnpq.br/3376562859305916Neuza Felix Gomes72157771387http://lattes.cnpq.br/665967015616205500164371362http://lattes.cnpq.br/6278753319766242Beatriz de Sousa e LimaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em BioquÃmicaUFCBRAnnona muricata L.Atividade antioxidanteAtividade anti-inflamatÃriaEdema de orelhaXileno TPAAntioxidantesPlantas medicinaisAnnona muricata L.Antioxidant activityAnti-inflammatory activityEar edemaXylene TPABIOQUIMICASoursop (Annona muricata L.) is a tree of Annonaceae family widely distributed in all tropical regions of the world, produces fruit known as graviola and its leaves are used in traditional medicine as phytotherapy. Some compounds present in A. muricata have been related to antioxidant activity, anti-inflammatory and antimicrobial activities of this plant. This study aimed to evaluate the antioxidant, anti-inflammatory and antibacterial activities of hexane (HE) and ethanol (EE) soursop leaves extracts. To this end, we used soursop leaves for preparation of extracts for further analyzes of phytochemical composition, antioxidant capacity, ear edema assays in vivo and in vitro antibacterial activity. For analysis of the antioxidant activity of hexane and ethanol extracts were determined total polyphenols, yellows flavonoids and anthocyanins and total antioxidant capacity by ABTS●+ and DPPH methods. To evaluate the anti-inflammatory activity of the extracts was performed ear edema model induced by xylene or TPA in mice (Swiss females, 25-35g)-treated intragastrically (ig) for 7 consecutive days or topically (single dose) of different concentrations of the extracts (EE or HE):10, 100 and 1000 mg/kg. The antimicrobial activity of the extracts was evaluated against strains of Staphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) and Salmonella choleraesuis (ATCC 14028). The extracts showed wide variety of phytochemical compounds, especially EE, and high antioxidant activity. Pretreatment via i.g. with the extracts was able to inhibit the edema induced by xylene, with a maximum percentage of inhibition of 61.72% for EE 10 and 57.08% for HE 100, but no effect on TPA-induced edema. The topical application with HE inhibited in a dose-dependent form the xylene-induced edema with maximum inhibition of 78.89% for HE1000, while for EE1000 was 67.95%, with no significant difference EE10 and EE100. As for the TPA-induced edema, HE (10, 100 and 1000 mg / kg) inhibited edema at 84.39%, 68.68% and 65.96%, while for EE (10, 100 and 1000 mg / kg ) the inhibition was 75%, 56% and 51%, respectively. Moreover, the extracts showed no antibacterial activity against all tested strains. The hexane and ethanol extracts of soursop leaves exhibit high differentiated antioxidant and anti-inflammatory activity compared to edema induced by xylene and TPA.A gravioleira (Annona muricata L.) à uma Ãrvore da famÃlia Annonaceae largamente distribuÃda em todas as regiÃes tropicais do mundo, produz fruto conhecido como graviola e suas folhas sÃo usadas na medicina tradicional como fitoterÃpicos. Alguns compostos presentes na A. muricata jà foram relacionados com as atividades antioxidante, anti-inflamatÃria e antimicrobiana dessa planta. Esse trabalho teve por objetivos avaliar as atividades antioxidante, anti-inflamatÃria e antibacteriana dos extratos hexÃnico (EH) e etanÃlico (EE) das folhas de gravioleira. Para tanto, foram utilizadas folhas de gravioleira para o preparo dos extratos utilizados em anÃlises da composiÃÃo fitoquÃmica, da capacidade antioxidante total, ensaios de edema de orelha in vivo e da atividade antibacteriana in vitro. Para a anÃlise da atividade antioxidante dos extratos hexÃnico e etanÃlico foram determinados os polifenÃis totais, flavonoides amarelos e antocianinas totais e a capacidade antioxidante total pelos mÃtodos ABTS●+ e DPPH. Para avaliaÃÃo da atividade anti-inflamatÃria dos extratos, foi utilizado o modelo de edema de orelha induzido por xileno ou TPA em camundongos (Swiss fÃmeas, 25-35g) submetidos a tratamentos por via intragÃstrica (i.g.) por 7 dias consecutivos ou por via tÃpica (dose Ãnica) com diferentes concentraÃÃes dos extratos (EH ou EE): 10, 100 e 1000 mg/kg. A atividade antimicrobiana dos extratos foi avaliada contra cepas de Sthaphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) e Salmonella choleraesuis (ATCC 14028). Os extratos apresentaram grande variedade de compostos fitoquÃmicos, principalmente o EE, alÃm de elevada atividade antioxidante. O prÃ-tratamento por via i.g. com os extratos foi capaz de inibir o edema induzido por xileno, com um percentual mÃximo de inibiÃÃo de 61,72% para o EE10 e de 57,08% para o EH100, mas nenhum efeito no edema induzido por TPA. O tratamento tÃpico por EH inibiu de forma dose-dependente o edema induzido por xileno, com mÃximo de inibiÃÃo de 78,89% para EH1000, enquanto para EE1000 foi de 67,95%, sem diferenÃa significativa com EE10 e EE100. Jà para o edema induzido por TPA, EH (10, 100 e 1000 mg/kg) inibiram o edema em 84,39%, 68,68% e 65,96%, enquanto para EE (10, 100 e 1000 mg/kg) a inibiÃÃo foi de 75%, 56% e 51%, respectivamente. Ademais, os extratos nÃo apresentaram atividade antibacteriana frente Ãs cepas testadas. Os extratos hexÃnico e etanÃlico das folhas de gravioleira apresentam elevada capacidade antioxidante e atividade anti-inflamatÃria diferenciada em relaÃÃo aos edemas induzidos por xileno e TPA. CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15629application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:29:05Zmail@mail.com - |
dc.title.en.fl_str_mv |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
dc.title.alternative.pt.fl_str_mv |
Atividades antioxidante, anti-inflamatÃria e antibacteriana dos extratos hexÃnico e etanÃlico das folhas da gravioleira (Annona muricata L.) |
title |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
spellingShingle |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) Beatriz de Sousa e Lima Annona muricata L. Atividade antioxidante Atividade anti-inflamatÃria Edema de orelha Xileno TPA Antioxidantes Plantas medicinais Annona muricata L. Antioxidant activity Anti-inflammatory activity Ear edema Xylene TPA BIOQUIMICA |
title_short |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
title_full |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
title_fullStr |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
title_full_unstemmed |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
title_sort |
Antioxidant activity, anti- inflammatory and antibacterial of hexane and ethanol extracts of the leaves of soursop (Annona muricata L.) |
author |
Beatriz de Sousa e Lima |
author_facet |
Beatriz de Sousa e Lima |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dirce Fernandes de Melo |
dc.contributor.advisor1ID.fl_str_mv |
02120178372 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6669454140368626 |
dc.contributor.advisor-co1.fl_str_mv |
Ãrika Freitas Mota |
dc.contributor.advisor-co1ID.fl_str_mv |
54454956391 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/5964165127429630 |
dc.contributor.referee1.fl_str_mv |
Diana CÃlia Sousa Nunes-Pinheiro |
dc.contributor.referee1ID.fl_str_mv |
15563278387 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3376562859305916 |
dc.contributor.referee2.fl_str_mv |
Neuza Felix Gomes |
dc.contributor.referee2ID.fl_str_mv |
72157771387 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6659670156162055 |
dc.contributor.authorID.fl_str_mv |
00164371362 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6278753319766242 |
dc.contributor.author.fl_str_mv |
Beatriz de Sousa e Lima |
contributor_str_mv |
Dirce Fernandes de Melo Ãrika Freitas Mota Diana CÃlia Sousa Nunes-Pinheiro Neuza Felix Gomes |
dc.subject.por.fl_str_mv |
Annona muricata L. Atividade antioxidante Atividade anti-inflamatÃria Edema de orelha Xileno TPA Antioxidantes Plantas medicinais |
topic |
Annona muricata L. Atividade antioxidante Atividade anti-inflamatÃria Edema de orelha Xileno TPA Antioxidantes Plantas medicinais Annona muricata L. Antioxidant activity Anti-inflammatory activity Ear edema Xylene TPA BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Annona muricata L. Antioxidant activity Anti-inflammatory activity Ear edema Xylene TPA |
dc.subject.cnpq.fl_str_mv |
BIOQUIMICA |
dc.description.sponsorship.fl_txt_mv |
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior |
dc.description.abstract.por.fl_txt_mv |
Soursop (Annona muricata L.) is a tree of Annonaceae family widely distributed in all tropical regions of the world, produces fruit known as graviola and its leaves are used in traditional medicine as phytotherapy. Some compounds present in A. muricata have been related to antioxidant activity, anti-inflammatory and antimicrobial activities of this plant. This study aimed to evaluate the antioxidant, anti-inflammatory and antibacterial activities of hexane (HE) and ethanol (EE) soursop leaves extracts. To this end, we used soursop leaves for preparation of extracts for further analyzes of phytochemical composition, antioxidant capacity, ear edema assays in vivo and in vitro antibacterial activity. For analysis of the antioxidant activity of hexane and ethanol extracts were determined total polyphenols, yellows flavonoids and anthocyanins and total antioxidant capacity by ABTS●+ and DPPH methods. To evaluate the anti-inflammatory activity of the extracts was performed ear edema model induced by xylene or TPA in mice (Swiss females, 25-35g)-treated intragastrically (ig) for 7 consecutive days or topically (single dose) of different concentrations of the extracts (EE or HE):10, 100 and 1000 mg/kg. The antimicrobial activity of the extracts was evaluated against strains of Staphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) and Salmonella choleraesuis (ATCC 14028). The extracts showed wide variety of phytochemical compounds, especially EE, and high antioxidant activity. Pretreatment via i.g. with the extracts was able to inhibit the edema induced by xylene, with a maximum percentage of inhibition of 61.72% for EE 10 and 57.08% for HE 100, but no effect on TPA-induced edema. The topical application with HE inhibited in a dose-dependent form the xylene-induced edema with maximum inhibition of 78.89% for HE1000, while for EE1000 was 67.95%, with no significant difference EE10 and EE100. As for the TPA-induced edema, HE (10, 100 and 1000 mg / kg) inhibited edema at 84.39%, 68.68% and 65.96%, while for EE (10, 100 and 1000 mg / kg ) the inhibition was 75%, 56% and 51%, respectively. Moreover, the extracts showed no antibacterial activity against all tested strains. The hexane and ethanol extracts of soursop leaves exhibit high differentiated antioxidant and anti-inflammatory activity compared to edema induced by xylene and TPA. A gravioleira (Annona muricata L.) à uma Ãrvore da famÃlia Annonaceae largamente distribuÃda em todas as regiÃes tropicais do mundo, produz fruto conhecido como graviola e suas folhas sÃo usadas na medicina tradicional como fitoterÃpicos. Alguns compostos presentes na A. muricata jà foram relacionados com as atividades antioxidante, anti-inflamatÃria e antimicrobiana dessa planta. Esse trabalho teve por objetivos avaliar as atividades antioxidante, anti-inflamatÃria e antibacteriana dos extratos hexÃnico (EH) e etanÃlico (EE) das folhas de gravioleira. Para tanto, foram utilizadas folhas de gravioleira para o preparo dos extratos utilizados em anÃlises da composiÃÃo fitoquÃmica, da capacidade antioxidante total, ensaios de edema de orelha in vivo e da atividade antibacteriana in vitro. Para a anÃlise da atividade antioxidante dos extratos hexÃnico e etanÃlico foram determinados os polifenÃis totais, flavonoides amarelos e antocianinas totais e a capacidade antioxidante total pelos mÃtodos ABTS●+ e DPPH. Para avaliaÃÃo da atividade anti-inflamatÃria dos extratos, foi utilizado o modelo de edema de orelha induzido por xileno ou TPA em camundongos (Swiss fÃmeas, 25-35g) submetidos a tratamentos por via intragÃstrica (i.g.) por 7 dias consecutivos ou por via tÃpica (dose Ãnica) com diferentes concentraÃÃes dos extratos (EH ou EE): 10, 100 e 1000 mg/kg. A atividade antimicrobiana dos extratos foi avaliada contra cepas de Sthaphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) e Salmonella choleraesuis (ATCC 14028). Os extratos apresentaram grande variedade de compostos fitoquÃmicos, principalmente o EE, alÃm de elevada atividade antioxidante. O prÃ-tratamento por via i.g. com os extratos foi capaz de inibir o edema induzido por xileno, com um percentual mÃximo de inibiÃÃo de 61,72% para o EE10 e de 57,08% para o EH100, mas nenhum efeito no edema induzido por TPA. O tratamento tÃpico por EH inibiu de forma dose-dependente o edema induzido por xileno, com mÃximo de inibiÃÃo de 78,89% para EH1000, enquanto para EE1000 foi de 67,95%, sem diferenÃa significativa com EE10 e EE100. Jà para o edema induzido por TPA, EH (10, 100 e 1000 mg/kg) inibiram o edema em 84,39%, 68,68% e 65,96%, enquanto para EE (10, 100 e 1000 mg/kg) a inibiÃÃo foi de 75%, 56% e 51%, respectivamente. Ademais, os extratos nÃo apresentaram atividade antibacteriana frente Ãs cepas testadas. Os extratos hexÃnico e etanÃlico das folhas de gravioleira apresentam elevada capacidade antioxidante e atividade anti-inflamatÃria diferenciada em relaÃÃo aos edemas induzidos por xileno e TPA. |
description |
Soursop (Annona muricata L.) is a tree of Annonaceae family widely distributed in all tropical regions of the world, produces fruit known as graviola and its leaves are used in traditional medicine as phytotherapy. Some compounds present in A. muricata have been related to antioxidant activity, anti-inflammatory and antimicrobial activities of this plant. This study aimed to evaluate the antioxidant, anti-inflammatory and antibacterial activities of hexane (HE) and ethanol (EE) soursop leaves extracts. To this end, we used soursop leaves for preparation of extracts for further analyzes of phytochemical composition, antioxidant capacity, ear edema assays in vivo and in vitro antibacterial activity. For analysis of the antioxidant activity of hexane and ethanol extracts were determined total polyphenols, yellows flavonoids and anthocyanins and total antioxidant capacity by ABTS●+ and DPPH methods. To evaluate the anti-inflammatory activity of the extracts was performed ear edema model induced by xylene or TPA in mice (Swiss females, 25-35g)-treated intragastrically (ig) for 7 consecutive days or topically (single dose) of different concentrations of the extracts (EE or HE):10, 100 and 1000 mg/kg. The antimicrobial activity of the extracts was evaluated against strains of Staphylococcus aureus (ATCC 14458), Listeria monocytogenes (ATCC 33090), Escherichia coli (ATCC 11775) and Salmonella choleraesuis (ATCC 14028). The extracts showed wide variety of phytochemical compounds, especially EE, and high antioxidant activity. Pretreatment via i.g. with the extracts was able to inhibit the edema induced by xylene, with a maximum percentage of inhibition of 61.72% for EE 10 and 57.08% for HE 100, but no effect on TPA-induced edema. The topical application with HE inhibited in a dose-dependent form the xylene-induced edema with maximum inhibition of 78.89% for HE1000, while for EE1000 was 67.95%, with no significant difference EE10 and EE100. As for the TPA-induced edema, HE (10, 100 and 1000 mg / kg) inhibited edema at 84.39%, 68.68% and 65.96%, while for EE (10, 100 and 1000 mg / kg ) the inhibition was 75%, 56% and 51%, respectively. Moreover, the extracts showed no antibacterial activity against all tested strains. The hexane and ethanol extracts of soursop leaves exhibit high differentiated antioxidant and anti-inflammatory activity compared to edema induced by xylene and TPA. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-03-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
format |
masterThesis |
dc.identifier.uri.fl_str_mv |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15629 |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15629 |
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por |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em BioquÃmica |
dc.publisher.initials.fl_str_mv |
UFC |
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BR |
publisher.none.fl_str_mv |
Universidade Federal do Cearà |
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reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
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Universidade Federal do Ceará |
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UFC |
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UFC |
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mail@mail.com |
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