Isolation of enterococos and fecais coliformes of oysters (Crassostrea rhizophorae) sold in the beach of the Futuro, Fortaleza, CearÃ.

• Autor(a) principal: Ana MÃrcia de Faria Morelli
• Data de Publicação: 2003
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
• Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=1738
• topic: Coliformes fecais; animais aquÃticos; invertebrados marinhos; microbiologia marinha; ostras; crustÃceo; CIENCIA E TECNOLOGIA DE ALIMENTOS

The Most Probable Numbers (NMPs) of enterococci and thermotolerant coliforms were estimated in 60 oyster samples (crassostrea rhizophorea) sold at two beach restaurantes, A and B, at Praia do Futuro, Fortaleza City, Cearà state, during the period between september 2000 and september 2001. Each sample included 25 individuals, therefore reaching a total of approximately 1,500 tested oyster. The enterococci NMPs in the samples collected at A ranged between <3,0/g and >1,100/g, while those sampled at B were between 3,0/g and >1,100/g. For the thermotolerant coliforms the obtained values were between <3,0/g and 9,300/g for A, and between <3,0/g and >110,000/g for B. Of all the tested samples, 40% of the oyster sampled at A and 43% of those sampled at B showed thermotolerant coliforms NMPs values over the limits established by the National Health Safety Agency ( AgÃncia Nacional de VigilÃncia SanitÃria) for human consumption of raw food (BRASIL, 2001). There was no statistical correlation between the enterococci and thermotolerant coliforms NMPs (p<0.05). 90 strains of E. faecalis (71%), 8 of E. hirae (6%), 2 of E. mundtii (2%) and 1 of E. durans (1%) were isolated at A, while at B 76 strains of E. faecalis (63%), 5 of E. hirae (4%), 2 of E. mundtii (2%) were found. Regarding the thermotolerant coliform group, 56 strains of E. coli (82%), 12 of Enterobacter (17%) and 1 of Citrobacter (1%) were confirmed at A, while 40 strains of E. coli (63%), 19 of Enterobacter (30%), 3 of Citrobacter (5%) and 1 of Klebsiella (2%) were found at B. All the E. coli strains isolated at A were sensitive to the following antimicrobial agents: CIP, CN, CRO, FOX, IMP, NA and SXT. 70% of these strains showed resistence to ampiciline. At B, the higher sensitivies (100%) were observed for the following antimicrobial agents: CIP, CN, CRO, FOX, IMP, NA and NIT. The ampiciline-resistence values were similar to those at A. None of the Enterococcus species showed to be capable to produce growth inhibiting substances (bacteriocins) aganist E. coli.