Utilization of green coconut shell (Cocos nucifera L.) for cellulase production
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6469 |
Resumo: | Enzyme production by fermentative process is a broad field of biotechnology. In recent decades, there has been an increasing trend in the use of solid-state fermentation (SSF) for the production of some enzymes, especially those involved in the degradation of vegetables complex macromolecules. Regarding the enzyme market Brazil is consumer of imported products. In this scenario, the coconut shell emerges as a strategic raw material to reach the production of enzymes in the country. Thus, it is important to further on the use of coconut green shell powder as substrate for cellulolytic enzymes of interest in the food industry. Cellulase producing fungi used in this study were isolated from coconut shell. The results of enzyme activity obtained with the isolated strains from coconut shell were compared in terms of production capacity of cellulases with strains of Trichoderma polysporum, T. viride, T. reesei NRRL 11460 and Aspergillus niger NRRL 2001. From the results, the coconut shell waste can be considered a good inducer for cellulase production . The strain isolated from coconut shell (CZ01) is a good producer under solid-state fermentation because higher enzyme activity was obtained with its crude enzyme extract when compared with other enzyme producers, including a patented strain of T. reesei Rut C-30 (NRRL 11460), using the same inducer substrate. It is also worth mentioning that the majority of cellulase reported in the scientific literature are obtained under conditions of acidic pH and in this study the maximum enzyme activity was obtained at pH close to neutral (range 6.0 to 6.5). Therefore, the isolated and selected strain from the coconut shell (CZ01) showed good enzyme activity when compared with results obtained by other strains reported in the scientific literature, being therefore a very promising strain for the cellulase production enabling the use of this agroindustrial wastes. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisUtilization of green coconut shell (Cocos nucifera L.) for cellulase productionAproveitamento da casca do coco verde (Cocos Nucifera, L.) para produÃÃo de celulases2010-02-26Sueli Rodrigues19633877830http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4707745Z6Claudia Miranda Martins00462326721http://lattes.cnpq.br/8875218219119631CÃlia Maria AraÃjo GalvÃo93808968400http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709028U375605309315http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4506945A8Simone Lopes do RÃgo de OliveiraUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em CiÃncia e Tecnologia de AlimentosUFCBRCIENCIA E TECNOLOGIA DE ALIMENTOSEnzyme production by fermentative process is a broad field of biotechnology. In recent decades, there has been an increasing trend in the use of solid-state fermentation (SSF) for the production of some enzymes, especially those involved in the degradation of vegetables complex macromolecules. Regarding the enzyme market Brazil is consumer of imported products. In this scenario, the coconut shell emerges as a strategic raw material to reach the production of enzymes in the country. Thus, it is important to further on the use of coconut green shell powder as substrate for cellulolytic enzymes of interest in the food industry. Cellulase producing fungi used in this study were isolated from coconut shell. The results of enzyme activity obtained with the isolated strains from coconut shell were compared in terms of production capacity of cellulases with strains of Trichoderma polysporum, T. viride, T. reesei NRRL 11460 and Aspergillus niger NRRL 2001. From the results, the coconut shell waste can be considered a good inducer for cellulase production . The strain isolated from coconut shell (CZ01) is a good producer under solid-state fermentation because higher enzyme activity was obtained with its crude enzyme extract when compared with other enzyme producers, including a patented strain of T. reesei Rut C-30 (NRRL 11460), using the same inducer substrate. It is also worth mentioning that the majority of cellulase reported in the scientific literature are obtained under conditions of acidic pH and in this study the maximum enzyme activity was obtained at pH close to neutral (range 6.0 to 6.5). Therefore, the isolated and selected strain from the coconut shell (CZ01) showed good enzyme activity when compared with results obtained by other strains reported in the scientific literature, being therefore a very promising strain for the cellulase production enabling the use of this agroindustrial wastes.A produÃÃo de enzimas por processos fermentativos à um vasto campo da biotecnologia. Nas Ãltimas dÃcadas tem-se observado um aumento na tendÃncia do uso da fermentaÃÃo semi-sÃlida para a produÃÃo de algumas enzimas, em especial aquelas envolvidas na degradaÃÃo de macromolÃculas vegetais complexas. No campo da comercializaÃÃo de enzimas, o Brasil à consumidor de produtos importados. Neste cenÃrio, a casca do coco verde surge como uma matÃria-prima estratÃgica para alavancar a produÃÃo de enzimas em territÃrio nacional. Deste modo, torna-se importante um estudo mais aprofundado sobre o uso do pà da casca do coco verde como substrato para a obtenÃÃo de enzimas celulolÃticas, sobretudo de interesse da indÃstria de alimentos. Os fungos produtores de celulases utilizados neste trabalho foram isolados da casca de coco. Os resultados de atividade enzimÃtica obtidos com as cepas isoladas da casca do coco foram comparados quanto à capacidade de produÃÃo de celulases com linhagens de Trichoderma polysporum, T. viride, T. reesei NRRL 11460 e Aspergillus niger NRRL 2001. O resÃduo da casca de coco verde como fonte alternativa para a produÃÃo de celulases se revelou como um Ãtimo substrato indutor, pois de acordo com os resultados obtidos, pode-se afirmar que a linhagem CZ01 (isolada da casca do coco) à boa produtora de celulases, pois cresceu facilmente em substrato semi-sÃlido lignificado, alÃm de apresentar elevada atividade no extrato enzimÃtico bruto, quando comparada com outros produtores enzimÃticos, incluindo uma linhagem patenteada de T. reesei Rut C-30 (NRRL 11460), utilizando o mesmo substrato indutor. Vale ressaltar tambÃm, que a grande maioria das celulases reportadas na literatura cientÃfica sÃo obtidas em condiÃÃes de pH Ãcido e neste estudo a mÃxima atividade enzimÃtica foi obtida na faixa de pH muito prÃximo da neutralidade (faixa de 6.0 a 6.5). Portanto, a linhagem selecionada e isolada da casca do coco (CZ01) apresentou boa atividade enzimÃtica quando comparada com resultados obtidos por outras linhagens reportadas na literatura cientÃfica, tratando-se, portanto, de uma linhagem bastante promissora para a produÃÃo de celulases viabilizando o aproveitamento deste resÃduo agroindustrial. Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicohttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6469application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:19:27Zmail@mail.com - |
dc.title.en.fl_str_mv |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
dc.title.alternative.pt.fl_str_mv |
Aproveitamento da casca do coco verde (Cocos Nucifera, L.) para produÃÃo de celulases |
title |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
spellingShingle |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production Simone Lopes do RÃgo de Oliveira CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
title_full |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
title_fullStr |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
title_full_unstemmed |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
title_sort |
Utilization of green coconut shell (Cocos nucifera L.) for cellulase production |
author |
Simone Lopes do RÃgo de Oliveira |
author_facet |
Simone Lopes do RÃgo de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Sueli Rodrigues |
dc.contributor.advisor1ID.fl_str_mv |
19633877830 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4707745Z6 |
dc.contributor.referee1.fl_str_mv |
Claudia Miranda Martins |
dc.contributor.referee1ID.fl_str_mv |
00462326721 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8875218219119631 |
dc.contributor.referee2.fl_str_mv |
CÃlia Maria AraÃjo GalvÃo |
dc.contributor.referee2ID.fl_str_mv |
93808968400 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709028U3 |
dc.contributor.authorID.fl_str_mv |
75605309315 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4506945A8 |
dc.contributor.author.fl_str_mv |
Simone Lopes do RÃgo de Oliveira |
contributor_str_mv |
Sueli Rodrigues Claudia Miranda Martins CÃlia Maria AraÃjo GalvÃo |
dc.subject.cnpq.fl_str_mv |
CIENCIA E TECNOLOGIA DE ALIMENTOS |
topic |
CIENCIA E TECNOLOGIA DE ALIMENTOS |
dc.description.sponsorship.fl_txt_mv |
Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico |
dc.description.abstract.por.fl_txt_mv |
Enzyme production by fermentative process is a broad field of biotechnology. In recent decades, there has been an increasing trend in the use of solid-state fermentation (SSF) for the production of some enzymes, especially those involved in the degradation of vegetables complex macromolecules. Regarding the enzyme market Brazil is consumer of imported products. In this scenario, the coconut shell emerges as a strategic raw material to reach the production of enzymes in the country. Thus, it is important to further on the use of coconut green shell powder as substrate for cellulolytic enzymes of interest in the food industry. Cellulase producing fungi used in this study were isolated from coconut shell. The results of enzyme activity obtained with the isolated strains from coconut shell were compared in terms of production capacity of cellulases with strains of Trichoderma polysporum, T. viride, T. reesei NRRL 11460 and Aspergillus niger NRRL 2001. From the results, the coconut shell waste can be considered a good inducer for cellulase production . The strain isolated from coconut shell (CZ01) is a good producer under solid-state fermentation because higher enzyme activity was obtained with its crude enzyme extract when compared with other enzyme producers, including a patented strain of T. reesei Rut C-30 (NRRL 11460), using the same inducer substrate. It is also worth mentioning that the majority of cellulase reported in the scientific literature are obtained under conditions of acidic pH and in this study the maximum enzyme activity was obtained at pH close to neutral (range 6.0 to 6.5). Therefore, the isolated and selected strain from the coconut shell (CZ01) showed good enzyme activity when compared with results obtained by other strains reported in the scientific literature, being therefore a very promising strain for the cellulase production enabling the use of this agroindustrial wastes. A produÃÃo de enzimas por processos fermentativos à um vasto campo da biotecnologia. Nas Ãltimas dÃcadas tem-se observado um aumento na tendÃncia do uso da fermentaÃÃo semi-sÃlida para a produÃÃo de algumas enzimas, em especial aquelas envolvidas na degradaÃÃo de macromolÃculas vegetais complexas. No campo da comercializaÃÃo de enzimas, o Brasil à consumidor de produtos importados. Neste cenÃrio, a casca do coco verde surge como uma matÃria-prima estratÃgica para alavancar a produÃÃo de enzimas em territÃrio nacional. Deste modo, torna-se importante um estudo mais aprofundado sobre o uso do pà da casca do coco verde como substrato para a obtenÃÃo de enzimas celulolÃticas, sobretudo de interesse da indÃstria de alimentos. Os fungos produtores de celulases utilizados neste trabalho foram isolados da casca de coco. Os resultados de atividade enzimÃtica obtidos com as cepas isoladas da casca do coco foram comparados quanto à capacidade de produÃÃo de celulases com linhagens de Trichoderma polysporum, T. viride, T. reesei NRRL 11460 e Aspergillus niger NRRL 2001. O resÃduo da casca de coco verde como fonte alternativa para a produÃÃo de celulases se revelou como um Ãtimo substrato indutor, pois de acordo com os resultados obtidos, pode-se afirmar que a linhagem CZ01 (isolada da casca do coco) à boa produtora de celulases, pois cresceu facilmente em substrato semi-sÃlido lignificado, alÃm de apresentar elevada atividade no extrato enzimÃtico bruto, quando comparada com outros produtores enzimÃticos, incluindo uma linhagem patenteada de T. reesei Rut C-30 (NRRL 11460), utilizando o mesmo substrato indutor. Vale ressaltar tambÃm, que a grande maioria das celulases reportadas na literatura cientÃfica sÃo obtidas em condiÃÃes de pH Ãcido e neste estudo a mÃxima atividade enzimÃtica foi obtida na faixa de pH muito prÃximo da neutralidade (faixa de 6.0 a 6.5). Portanto, a linhagem selecionada e isolada da casca do coco (CZ01) apresentou boa atividade enzimÃtica quando comparada com resultados obtidos por outras linhagens reportadas na literatura cientÃfica, tratando-se, portanto, de uma linhagem bastante promissora para a produÃÃo de celulases viabilizando o aproveitamento deste resÃduo agroindustrial. |
description |
Enzyme production by fermentative process is a broad field of biotechnology. In recent decades, there has been an increasing trend in the use of solid-state fermentation (SSF) for the production of some enzymes, especially those involved in the degradation of vegetables complex macromolecules. Regarding the enzyme market Brazil is consumer of imported products. In this scenario, the coconut shell emerges as a strategic raw material to reach the production of enzymes in the country. Thus, it is important to further on the use of coconut green shell powder as substrate for cellulolytic enzymes of interest in the food industry. Cellulase producing fungi used in this study were isolated from coconut shell. The results of enzyme activity obtained with the isolated strains from coconut shell were compared in terms of production capacity of cellulases with strains of Trichoderma polysporum, T. viride, T. reesei NRRL 11460 and Aspergillus niger NRRL 2001. From the results, the coconut shell waste can be considered a good inducer for cellulase production . The strain isolated from coconut shell (CZ01) is a good producer under solid-state fermentation because higher enzyme activity was obtained with its crude enzyme extract when compared with other enzyme producers, including a patented strain of T. reesei Rut C-30 (NRRL 11460), using the same inducer substrate. It is also worth mentioning that the majority of cellulase reported in the scientific literature are obtained under conditions of acidic pH and in this study the maximum enzyme activity was obtained at pH close to neutral (range 6.0 to 6.5). Therefore, the isolated and selected strain from the coconut shell (CZ01) showed good enzyme activity when compared with results obtained by other strains reported in the scientific literature, being therefore a very promising strain for the cellulase production enabling the use of this agroindustrial wastes. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
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masterThesis |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6469 |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6469 |
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openAccess |
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Universidade Federal do Cearà |
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Programa de PÃs-GraduaÃÃo em CiÃncia e Tecnologia de Alimentos |
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UFC |
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BR |
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Universidade Federal do Cearà |
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reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Universidade Federal do Ceará |
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UFC |
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UFC |
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mail@mail.com |
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