Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/11239 |
Resumo: | The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), is one of the most harmful species to world horticulture, thus requiring suppression or eradication programs. The sterile insect technique is widely used for autocidal control of nuisance insects because it neither releases exotic agents into the environment nor introduces new genetic material into existing populations. In 2005, the Moscamed Brasil biofactory imported the tsl Vienna 8 C. capitata strain from the International Atomic Energy Agency (IAEA); this action required genetic testing of the strain’s biological characteristics. The objective of this study was to discriminate males of wild populations from tsl Vienna 8 using molecular markers, and to conclude which male line dominated in a remating condition. Four crosses, each using 100 males and 100 females, were performed: wild female × wild male; wild female × tsl Vienna 8 male; wild female × wild male, followed by remating with tsl Vienna 8 males after 48 h, and wild female × tsl Vienna 8 male, followed by remating with wild males after 48 h. The results showed that the tsl Vienna 8 strain is compatible with wild females from the São Francisco Valley region and that these males can successfully transfer their sperm to the female spermathecae. Regarding remating, the sperm of the second male dominated over that of the first one. Based on these findings, the sterile insect technique success may be reduced, since the progeny of a female that remates with a wild male could be viable. |
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Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approachInfluência da recópula na técnica do inseto estéril em Ceratitis capitata (Diptera: Tephritidae): uma abordagem molecularCriação massal. Espermateca. Fruticultura. Marcadores genéticos. Moscamed.Genetic markers. Fruitgrowing. Mass rearing. Medfly. Spermathecae.The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), is one of the most harmful species to world horticulture, thus requiring suppression or eradication programs. The sterile insect technique is widely used for autocidal control of nuisance insects because it neither releases exotic agents into the environment nor introduces new genetic material into existing populations. In 2005, the Moscamed Brasil biofactory imported the tsl Vienna 8 C. capitata strain from the International Atomic Energy Agency (IAEA); this action required genetic testing of the strain’s biological characteristics. The objective of this study was to discriminate males of wild populations from tsl Vienna 8 using molecular markers, and to conclude which male line dominated in a remating condition. Four crosses, each using 100 males and 100 females, were performed: wild female × wild male; wild female × tsl Vienna 8 male; wild female × wild male, followed by remating with tsl Vienna 8 males after 48 h, and wild female × tsl Vienna 8 male, followed by remating with wild males after 48 h. The results showed that the tsl Vienna 8 strain is compatible with wild females from the São Francisco Valley region and that these males can successfully transfer their sperm to the female spermathecae. Regarding remating, the sperm of the second male dominated over that of the first one. Based on these findings, the sterile insect technique success may be reduced, since the progeny of a female that remates with a wild male could be viable.A mosca-das-frutas do Mediterrâneo, Ceratitis capitata (Diptera: Tephritidae), é uma das espécies mais prejudiciais à horticultura mundial, portanto requer programas de supressão ou erradicação. A técnica do inseto estéril é amplamente utilizada para o controle autocida de insetos nocivos, pois não libera agentes exóticos no meio ambiente nem introduz novos materiais genéticos nas populações existentes. Em 2005, a biofábrica Moscamed Brasil importou a cepa Vienna 8 C. capitata da Agência Internacional de Energia Atômica (IAEA); essa ação exigiu testes genéticos das características biológicas da linhagem. O objetivo deste estudo foi discriminar machos de populações silvestres de tsl Viena 8 usando marcadores moleculares, e inferir sobre qual linhagem masculina era dominante em condição de recópula. Quatro cruzamentos, cada um com 100 machos e 100 fêmeas, foram realizados: fêmea selvagem × macho selvagem; fêmea selvagem × macho tsl Viena 8; fêmea selvagem × macho selvagem, seguido por recópula com machos tsl Vienna 8 após 48 h, e fêmea selvagem × machos tsl Vienna 8, seguido por recópula com machos selvagens após 48 h. Os resultados mostraram que a cepa tsl Vienna 8 é compatível com fêmeas selvagens da região do Vale do São Francisco e que esses machos podem transferir com sucesso seus espermatozóides para a espermateca das fêmeas. Quanto à recópula, o esperma do segundo macho precedeu o primeiro. Com base nesses achados, o sucesso da técnica de insetos estéril pode ser reduzido, já que a progênie de uma fêmea que recopulou com um macho selvagem pode ser viável.Universidade Federal Rural do Semi-Árido2023-07-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1123910.1590/1983-21252023v36n308rcREVISTA CAATINGA; Vol. 36 No. 3 (2023); 553-560Revista Caatinga; v. 36 n. 3 (2023); 553-5601983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/11239/11282Copyright (c) 2023 Revista Caatingainfo:eu-repo/semantics/openAccessBarboza, Vilmara PereiraParanhos, Beatriz Aguiar JordãoSilva junior, Juvenal Cordeiro2023-07-18T12:27:59Zoai:ojs.periodicos.ufersa.edu.br:article/11239Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:47:02.612916Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach Influência da recópula na técnica do inseto estéril em Ceratitis capitata (Diptera: Tephritidae): uma abordagem molecular |
title |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
spellingShingle |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach Barboza, Vilmara Pereira Criação massal. Espermateca. Fruticultura. Marcadores genéticos. Moscamed. Genetic markers. Fruitgrowing. Mass rearing. Medfly. Spermathecae. |
title_short |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
title_full |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
title_fullStr |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
title_full_unstemmed |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
title_sort |
Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach |
author |
Barboza, Vilmara Pereira |
author_facet |
Barboza, Vilmara Pereira Paranhos, Beatriz Aguiar Jordão Silva junior, Juvenal Cordeiro |
author_role |
author |
author2 |
Paranhos, Beatriz Aguiar Jordão Silva junior, Juvenal Cordeiro |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Barboza, Vilmara Pereira Paranhos, Beatriz Aguiar Jordão Silva junior, Juvenal Cordeiro |
dc.subject.por.fl_str_mv |
Criação massal. Espermateca. Fruticultura. Marcadores genéticos. Moscamed. Genetic markers. Fruitgrowing. Mass rearing. Medfly. Spermathecae. |
topic |
Criação massal. Espermateca. Fruticultura. Marcadores genéticos. Moscamed. Genetic markers. Fruitgrowing. Mass rearing. Medfly. Spermathecae. |
description |
The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), is one of the most harmful species to world horticulture, thus requiring suppression or eradication programs. The sterile insect technique is widely used for autocidal control of nuisance insects because it neither releases exotic agents into the environment nor introduces new genetic material into existing populations. In 2005, the Moscamed Brasil biofactory imported the tsl Vienna 8 C. capitata strain from the International Atomic Energy Agency (IAEA); this action required genetic testing of the strain’s biological characteristics. The objective of this study was to discriminate males of wild populations from tsl Vienna 8 using molecular markers, and to conclude which male line dominated in a remating condition. Four crosses, each using 100 males and 100 females, were performed: wild female × wild male; wild female × tsl Vienna 8 male; wild female × wild male, followed by remating with tsl Vienna 8 males after 48 h, and wild female × tsl Vienna 8 male, followed by remating with wild males after 48 h. The results showed that the tsl Vienna 8 strain is compatible with wild females from the São Francisco Valley region and that these males can successfully transfer their sperm to the female spermathecae. Regarding remating, the sperm of the second male dominated over that of the first one. Based on these findings, the sterile insect technique success may be reduced, since the progeny of a female that remates with a wild male could be viable. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11239 10.1590/1983-21252023v36n308rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/11239 |
identifier_str_mv |
10.1590/1983-21252023v36n308rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11239/11282 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 36 No. 3 (2023); 553-560 Revista Caatinga; v. 36 n. 3 (2023); 553-560 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674030015184896 |