Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/10663 |
Resumo: | This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl – APM (0, 10, 20, 30, 40, and 60 µM L–1) and colchicine (2.5 mM L–1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy. |
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Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy inductionEstimativa da ploidia e pré-seleção de autotetraploides de bananeira após indução da poliploidia in vitroMusa acuminata. Massa fresca foliar. Densidade estomática. Amiprofós-metil (APM). Colchicina.Musa acuminata. Leaf fresh mass. Stomatal density. Amiprofos-methyl (APM). Colchicine.This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl – APM (0, 10, 20, 30, 40, and 60 µM L–1) and colchicine (2.5 mM L–1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy.Este estudo objetivou avaliar a eficiência dos parâmetros massa fresca foliar e densidade estomática na estimativa da ploidia e pré-seleção de autotetraploides putativos de bananeira. Plantas jovens da cultivar diploide Ouro (AA), previamente submetidas à poliploidização in vitro com os antimitóticos amiprofos-metil - APM (0, 10, 20, 30, 40 e 60 µM L-1) e colchicina (2,5 mM L-1) foram avaliadas quanto à sobrevivência, altura, número de folhas, diâmetro do pseudocaule, massa fresca de disco foliar e densidade estomática. Determinou-se a ploidia, via citometria de fluxo, numa amostra aleatória de 200 plantas, a fim de encontrar relação entre o conteúdo genômico e as variáveis analisadas. Por meio da análise de correlação de Spearman, verificou-se forte correlação (0,84**) entre a massa fresca de disco foliar e as plantas tetraploides, bem como ausência de correlação significativa entre a densidade estomática e os níveis de ploidia. Assim, a pré-seleção das plantas foi feita mediante um valor de referência de massa fresca de disco foliar e resultou em 688 plantas pré-selecionadas como tetraploides putativos. Destas, 318 foram confirmadas como tetraploides, 291 como diploides e 79 mixoploides, após análise de citometria de fluxo. A eficiência de pré-seleção foi de 46,2% de plantas tetraploides do total pré-selecionado. Os resultados indicam que o uso da massa fresca de disco foliar é um método simples, prático e promissor para estimar a ploidia quando é obtido um elevado número de plantas em trabalhos de poliploidização in vitro da bananeira, reduzindo o total de plantas a ter a ploidia confirmada.Universidade Federal Rural do Semi-Árido2023-07-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1066310.1590/1983-21252023v36n302rcREVISTA CAATINGA; Vol. 36 No. 3 (2023); 494-501Revista Caatinga; v. 36 n. 3 (2023); 494-5011983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/10663/11276Copyright (c) 2023 Revista Caatingainfo:eu-repo/semantics/openAccessBorges, Viviane PeixotoSilveira, Daniela GarciaCosta, Maria Angélica Pereira de CarvalhoSantos-Serejo, Janay Almeida dosSilva, Sebastião de Oliveira e 2023-07-18T12:28:06Zoai:ojs.periodicos.ufersa.edu.br:article/10663Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:57.811067Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction Estimativa da ploidia e pré-seleção de autotetraploides de bananeira após indução da poliploidia in vitro |
title |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
spellingShingle |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction Borges, Viviane Peixoto Musa acuminata. Massa fresca foliar. Densidade estomática. Amiprofós-metil (APM). Colchicina. Musa acuminata. Leaf fresh mass. Stomatal density. Amiprofos-methyl (APM). Colchicine. |
title_short |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
title_full |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
title_fullStr |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
title_full_unstemmed |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
title_sort |
Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction |
author |
Borges, Viviane Peixoto |
author_facet |
Borges, Viviane Peixoto Silveira, Daniela Garcia Costa, Maria Angélica Pereira de Carvalho Santos-Serejo, Janay Almeida dos Silva, Sebastião de Oliveira e |
author_role |
author |
author2 |
Silveira, Daniela Garcia Costa, Maria Angélica Pereira de Carvalho Santos-Serejo, Janay Almeida dos Silva, Sebastião de Oliveira e |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Borges, Viviane Peixoto Silveira, Daniela Garcia Costa, Maria Angélica Pereira de Carvalho Santos-Serejo, Janay Almeida dos Silva, Sebastião de Oliveira e |
dc.subject.por.fl_str_mv |
Musa acuminata. Massa fresca foliar. Densidade estomática. Amiprofós-metil (APM). Colchicina. Musa acuminata. Leaf fresh mass. Stomatal density. Amiprofos-methyl (APM). Colchicine. |
topic |
Musa acuminata. Massa fresca foliar. Densidade estomática. Amiprofós-metil (APM). Colchicina. Musa acuminata. Leaf fresh mass. Stomatal density. Amiprofos-methyl (APM). Colchicine. |
description |
This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl – APM (0, 10, 20, 30, 40, and 60 µM L–1) and colchicine (2.5 mM L–1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/10663 10.1590/1983-21252023v36n302rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/10663 |
identifier_str_mv |
10.1590/1983-21252023v36n302rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/10663/11276 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 36 No. 3 (2023); 494-501 Revista Caatinga; v. 36 n. 3 (2023); 494-501 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674029538082816 |