GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA

Detalhes bibliográficos
Autor(a) principal: Silveira, Amanda Letícia da
Data de Publicação: 2019
Outros Autores: Soares, Maria Gilmara de Oliveira, Lopes, Lucas Silveira, Stracieri, Juliana, Alves, Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Caatinga
Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/7526
Resumo: Colletotrichum spp. is a pathogenic fungus of great importance in banana cultivation since it affects the fruit both in the field and in the post-harvest period, depreciating its commercial value. Although this disease is widely distributed in regions of banana cultivation, the different levels of aggression suggest the existence of genetic variability among Colletotrichum spp. isolates. The objective of the present study was therefore to verify the genetic diversity of Colletotrichum spp. in banana fruits presenting anthracnose symptoms using both molecular marker ISSR and morphological characterization. We obtained 30 isolates of the fungus from banana fruits from different states of Brazil and performed molecular characterization with five primers using the ISSR-PCR technique. The results were used to construct a dendrogram, relating the isolates according to their genetic proximity. The morphocultural characterization was performed by analyzing colony coloration and growth rate. These methods allowed us to reveal genetic and morphocultural variability among isolates.
id UFERSA-1_2153e8026b3f86baf3cfdca750e3ebc1
oai_identifier_str oai:ojs.periodicos.ufersa.edu.br:article/7526
network_acronym_str UFERSA-1
network_name_str Revista Caatinga
repository_id_str
spelling GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANADIVERSIDADE GENÉTICA DE ISOLADOS DE Colletotrichum spp. EM BANANAVariability. ISSR-PCR. Musa spp.Variabilidade. Caracterização molecular. Musa spp.Colletotrichum spp. is a pathogenic fungus of great importance in banana cultivation since it affects the fruit both in the field and in the post-harvest period, depreciating its commercial value. Although this disease is widely distributed in regions of banana cultivation, the different levels of aggression suggest the existence of genetic variability among Colletotrichum spp. isolates. The objective of the present study was therefore to verify the genetic diversity of Colletotrichum spp. in banana fruits presenting anthracnose symptoms using both molecular marker ISSR and morphological characterization. We obtained 30 isolates of the fungus from banana fruits from different states of Brazil and performed molecular characterization with five primers using the ISSR-PCR technique. The results were used to construct a dendrogram, relating the isolates according to their genetic proximity. The morphocultural characterization was performed by analyzing colony coloration and growth rate. These methods allowed us to reveal genetic and morphocultural variability among isolates.Colletotrichum spp. é um fungo patogênico de grande importância na cultura da bananeira uma vez que afeta o fruto tanto no campo quanto no período pós-colheita, depreciando seu valor comercial. Essa doença está amplamente distribuída nas regiões onde a bananeira é cultivada, entretanto há diferentes níveis de agressividade da mesma, sugerindo a existência de variabilidade genética entre isolados de Colletotrichum spp. Diante do exposto, o objetivo do presente estudo foi verificar a diversidade genética de isolados de Colletotrichum spp. associados a frutos de banana, apresentando sintomas de antracnose por meio de marcador molecular ISSR e caracterização morfológica. Foram utilizados 30 isolados do fungo, obtidos de frutos de bananeira de quatro Estados do país. A caracterização molecular foi feita pela técnica ISSR-PCR, utilizando cinco primers. Com os resultados obtidos, foi construído um dendrograma, relacionando os isolados de acordo com a sua proximidade genética. A caracterização morfocultural foi realizada por meio da avaliação da coloração da colônia e da velocidade de crescimento. Foi possível verificar a existência de variabilidade genética e morfocultural entre os isolados. Os marcadores ISSR foram eficientes na caracterização de isolados de Colletotrichum spp. associadas a frutos de bananeira.Universidade Federal Rural do Semi-Árido2019-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/752610.1590/1983-21252019v32n304rcREVISTA CAATINGA; Vol. 32 No. 3 (2019); 599-606Revista Caatinga; v. 32 n. 3 (2019); 599-6061983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/7526/10022Copyright (c) 2019 Revista Caatingainfo:eu-repo/semantics/openAccessSilveira, Amanda Letícia daSoares, Maria Gilmara de OliveiraLopes, Lucas SilveiraStracieri, JulianaAlves, Eduardo2023-07-20T17:31:52Zoai:ojs.periodicos.ufersa.edu.br:article/7526Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:35.470934Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true
dc.title.none.fl_str_mv GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
DIVERSIDADE GENÉTICA DE ISOLADOS DE Colletotrichum spp. EM BANANA
title GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
spellingShingle GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
Silveira, Amanda Letícia da
Variability. ISSR-PCR. Musa spp.
Variabilidade. Caracterização molecular. Musa spp.
title_short GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
title_full GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
title_fullStr GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
title_full_unstemmed GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
title_sort GENETIC DIVERSITY OF ISOLATES OF Colletotrichum spp. IN BANANA
author Silveira, Amanda Letícia da
author_facet Silveira, Amanda Letícia da
Soares, Maria Gilmara de Oliveira
Lopes, Lucas Silveira
Stracieri, Juliana
Alves, Eduardo
author_role author
author2 Soares, Maria Gilmara de Oliveira
Lopes, Lucas Silveira
Stracieri, Juliana
Alves, Eduardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silveira, Amanda Letícia da
Soares, Maria Gilmara de Oliveira
Lopes, Lucas Silveira
Stracieri, Juliana
Alves, Eduardo
dc.subject.por.fl_str_mv Variability. ISSR-PCR. Musa spp.
Variabilidade. Caracterização molecular. Musa spp.
topic Variability. ISSR-PCR. Musa spp.
Variabilidade. Caracterização molecular. Musa spp.
description Colletotrichum spp. is a pathogenic fungus of great importance in banana cultivation since it affects the fruit both in the field and in the post-harvest period, depreciating its commercial value. Although this disease is widely distributed in regions of banana cultivation, the different levels of aggression suggest the existence of genetic variability among Colletotrichum spp. isolates. The objective of the present study was therefore to verify the genetic diversity of Colletotrichum spp. in banana fruits presenting anthracnose symptoms using both molecular marker ISSR and morphological characterization. We obtained 30 isolates of the fungus from banana fruits from different states of Brazil and performed molecular characterization with five primers using the ISSR-PCR technique. The results were used to construct a dendrogram, relating the isolates according to their genetic proximity. The morphocultural characterization was performed by analyzing colony coloration and growth rate. These methods allowed us to reveal genetic and morphocultural variability among isolates.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/7526
10.1590/1983-21252019v32n304rc
url https://periodicos.ufersa.edu.br/caatinga/article/view/7526
identifier_str_mv 10.1590/1983-21252019v32n304rc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/7526/10022
dc.rights.driver.fl_str_mv Copyright (c) 2019 Revista Caatinga
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Revista Caatinga
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
dc.source.none.fl_str_mv REVISTA CAATINGA; Vol. 32 No. 3 (2019); 599-606
Revista Caatinga; v. 32 n. 3 (2019); 599-606
1983-2125
0100-316X
reponame:Revista Caatinga
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Revista Caatinga
collection Revista Caatinga
repository.name.fl_str_mv Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv patricio@ufersa.edu.br|| caatinga@ufersa.edu.br
_version_ 1797674027221778432