MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/9938 |
Resumo: | Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues. |
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MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE)MODULAÇÃO DO MEIO DE CULTURA NA CONSERVAÇÃO EX SITU DE Neoregelia mucugensis Leme (BROMELIACEAE)Crescimento lento. Bromélia. Osmorregulador.Slow growth. Bromeliad. Osmoregulators.Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues.As bromélias são alvo de extrativismo predatório e com isso muitas estão inseridas na lista vermelha de espécies ameaçadas, a exemplo das que pertencem ao gênero Neoregelia. Apesar da Neoregelia mucugensis não ter sido avaliada quanto ao grau de ameaça, sua exploração é exclusivamente extrativista e sua ocorrência na Chapada Diamantina-BA está sujeita à ação das queimadas que afetam a região anualmente. Neste contexto, a aplicação de técnicas que visem proteger este recurso genético é fundamental tanto para manutenção de suas populações naturais quanto para conservação ex situ deste material genético. Técnicas de cultura de tecidos vegetais têm sido aplicadas com êxito para conservação de diversas espécies de bromélias. Um dos métodos utilizados é o crescimento lento que consiste na redução do metabolismo da planta e consequentemente na desaceleração do seu crescimento, o que possibilita a manutenção das coleções de plantas in vitro sem necessidade de subcultivo. Neste sentido, o objetivo deste trabalho foi testar a redução de sais do meio de cultura e a adição de osmorregulador na indução do crescimento lento de N. mucugensis. Plantas foram submetidas a tratamentos compostos por diferentes concentrações do meio MS e de manitol pelo período de 12 meses, quando então foram realizadas as análises de crescimento, teor de clorofila e capacidade de regeneração de brotos in vitro. Constatou-se que o tratamento contendo MS ½ e 7,8 g.L-1 de manitol é indicado para a conservação in vitro de N. mucugensis com manutenção da capacidade regenerativa dos seus tecidos. Universidade Federal Rural do Semi-Árido2021-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/993810.1590/1983-21252021v34n403rcREVISTA CAATINGA; Vol. 34 No. 4 (2021); 763-771Revista Caatinga; v. 34 n. 4 (2021); 763-7711983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/9938/10738Copyright (c) 2021 Revista Caatingainfo:eu-repo/semantics/openAccessLima, Andressa Priscila Piancó SantosBastos, Fernanda de Jesus OliveiraLima-Brito, AloneFernandes, Gilênio BorgesSantana, José Raniere Ferreira de2023-07-04T13:30:02Zoai:ojs.periodicos.ufersa.edu.br:article/9938Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:54.223651Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) MODULAÇÃO DO MEIO DE CULTURA NA CONSERVAÇÃO EX SITU DE Neoregelia mucugensis Leme (BROMELIACEAE) |
title |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
spellingShingle |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) Lima, Andressa Priscila Piancó Santos Crescimento lento. Bromélia. Osmorregulador. Slow growth. Bromeliad. Osmoregulators. |
title_short |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
title_full |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
title_fullStr |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
title_full_unstemmed |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
title_sort |
MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE) |
author |
Lima, Andressa Priscila Piancó Santos |
author_facet |
Lima, Andressa Priscila Piancó Santos Bastos, Fernanda de Jesus Oliveira Lima-Brito, Alone Fernandes, Gilênio Borges Santana, José Raniere Ferreira de |
author_role |
author |
author2 |
Bastos, Fernanda de Jesus Oliveira Lima-Brito, Alone Fernandes, Gilênio Borges Santana, José Raniere Ferreira de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lima, Andressa Priscila Piancó Santos Bastos, Fernanda de Jesus Oliveira Lima-Brito, Alone Fernandes, Gilênio Borges Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Crescimento lento. Bromélia. Osmorregulador. Slow growth. Bromeliad. Osmoregulators. |
topic |
Crescimento lento. Bromélia. Osmorregulador. Slow growth. Bromeliad. Osmoregulators. |
description |
Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9938 10.1590/1983-21252021v34n403rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/9938 |
identifier_str_mv |
10.1590/1983-21252021v34n403rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9938/10738 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 34 No. 4 (2021); 763-771 Revista Caatinga; v. 34 n. 4 (2021); 763-771 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
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1797674029077757952 |