INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/9617 |
Resumo: | Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew–resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant. |
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INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)INDUÇÃO DE POLIPLOIDIA EM GENÓTIPOS DE MELANCIA COM RESISTÊNCIA AO OÍDIO (Podosphaera xanthii)Duplicação cromossômica. Tetraploidia. Citogenética. Citrullus lanatus.Chromosomal duplication. Tetraploidy. Cytogenetics. Citrullus lanatus.Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew–resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.A melancia triploide é muito apreciada pelos mercados mais exigentes, e, devido ao seu tamanho reduzido, torna-se ideal para consumo em famílias pequenas. Com o incremento de áreas cultivadas com melancia sem sementes no mundo, há demanda pelo desenvolvimento de germoplasma tetraploides para obtenção de híbridos triploides de melhor desempenho agronômico. Foram realizados dois ensaios com o objetivo de induzir poliploidia na Linhagem resistente ao oídio desenvolvida pela Embrapa Semiárido, LDRO, sob diferentes concentrações de colchicina e métodos de aplicação. As sementes foram tratadas com colchicina (0,0%; 0,1% e 0,2%) por 24h e 48h (Experimento 1) e sob diferentes métodos de aplicação de colchicina a 0,2% a) direto na semente (MDS) com e sem escarificação, b) sementes com emissão da radícula (MER), c) no ponto de inserção entre o hipocótilo e a raiz (MIHR), d) no ápice da plântula (MAP) e e) no hipocótilo invertido (MHI) (Experimento 2). Foi realizada a análise citogenética (contagem do número cromossômico), do número de cloroplastos por pares de células-guardas, número de estômatos, altura da plântula e diâmetro do hipocótilo. Na Linhagem LDRO, a duplicação cromossômica ocorreu em algumas células das plantas, mas não foi possível obter plantas 100% tetraploides. A colchicina a 0,2% por 48h sem escarificação mecânica induz a duplicação cromossômica em melancia. A análise do número de cloroplastos identifica os níveis de ploidia, de forma precoce, reduzindo o número de plantas, que serão avaliadas em citogenética, sendo que esta permite identificar com maior precisão os diferentes níveis de ploidia da planta.Universidade Federal Rural do Semi-Árido2022-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/961710.1590/1983-21252022v35n301rcREVISTA CAATINGA; Vol. 35 No. 3 (2022); 505-513Revista Caatinga; v. 35 n. 3 (2022); 505-5131983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/9617/10980Copyright (c) 2022 Revista Caatingainfo:eu-repo/semantics/openAccessSilva, Carla Maria de JesusDias, Rita de Cássia SouzaSantos, Joice Simone dosSouza, Flávio de FrançaMelo, Natoniel Franklin de2023-07-03T13:54:38Zoai:ojs.periodicos.ufersa.edu.br:article/9617Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:51.426906Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) INDUÇÃO DE POLIPLOIDIA EM GENÓTIPOS DE MELANCIA COM RESISTÊNCIA AO OÍDIO (Podosphaera xanthii) |
title |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
spellingShingle |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) Silva, Carla Maria de Jesus Duplicação cromossômica. Tetraploidia. Citogenética. Citrullus lanatus. Chromosomal duplication. Tetraploidy. Cytogenetics. Citrullus lanatus. |
title_short |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
title_full |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
title_fullStr |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
title_full_unstemmed |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
title_sort |
INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii) |
author |
Silva, Carla Maria de Jesus |
author_facet |
Silva, Carla Maria de Jesus Dias, Rita de Cássia Souza Santos, Joice Simone dos Souza, Flávio de França Melo, Natoniel Franklin de |
author_role |
author |
author2 |
Dias, Rita de Cássia Souza Santos, Joice Simone dos Souza, Flávio de França Melo, Natoniel Franklin de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Carla Maria de Jesus Dias, Rita de Cássia Souza Santos, Joice Simone dos Souza, Flávio de França Melo, Natoniel Franklin de |
dc.subject.por.fl_str_mv |
Duplicação cromossômica. Tetraploidia. Citogenética. Citrullus lanatus. Chromosomal duplication. Tetraploidy. Cytogenetics. Citrullus lanatus. |
topic |
Duplicação cromossômica. Tetraploidia. Citogenética. Citrullus lanatus. Chromosomal duplication. Tetraploidy. Cytogenetics. Citrullus lanatus. |
description |
Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew–resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9617 10.1590/1983-21252022v35n301rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/9617 |
identifier_str_mv |
10.1590/1983-21252022v35n301rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9617/10980 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 35 No. 3 (2022); 505-513 Revista Caatinga; v. 35 n. 3 (2022); 505-513 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674028948783104 |