INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS

Detalhes bibliográficos
Autor(a) principal: Borges, Neilson Oliveira
Data de Publicação: 2022
Outros Autores: Solino, Antônio Jussiê da Silva, Francischini, Ricardo, Oliveira, Juliana Santos Batista, Schwan-Estrada, Kátia Regina Freitas, Campos, Hercules Diniz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Caatinga
Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/9692
Resumo: The biological control, thinking about the integrated management, has been inserted with other management techniques to disease control, such as soybean anthracnose. The aims of this work were to verify the action of Trichoderma and Bacillus isolates in the induction of soybean resistance mechanisms to anthracnose as a function of seed treatment. The statistical design was entirely randomised, in a 5 x 2 (agent species x sampling times) factorial scheme with five replicates. Soybean seeds were treated with Bacillus amyloliquefaciens BV03, B. subtilis BV02, Trichoderma asperellum BV10, Carbendazim + Thiram and distilled water (control). Seven days after seedling emergence, 2 µL of 1 x 10-4 Colletotrichum truncatum spores were inoculated on the cotyledons. Catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and glyceollin (GLY) activities before and after pathogen inoculation, as well as the diameter of the anthracnose lesion on the cotyledons, were evaluated. Data were submitted to analysis of variance and, when significant, the mean values were compared by Fisher’s test (p < 0.05). The treatments did not influence the first sampling time before inoculation. Trichoderma asperellum BV10 increased POX and PAL activities up to 173%, while B. amyloliquefaciens BV03 increased POX activity. Glyceollin was not influenced by the treatments. The T. asperellum BV10 reduces the diameter of the anthracnose lesion by up to 61%. Thus, T. asperellum BV10 has the potential to control soybean anthracnose, improved the response defense against C. truncatum, when performed on seed treatment.
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spelling INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTSINDUÇÃO DE MECANISMOS DE RESISTÊNCIA DE SOJA À ANTRACNOSE COM AGENTES DE BIOCONTROLEColletotrichum truncatum. Glycine max. Indução de resistência.Colletotrichum truncatum. Glycine max. Resistance induction.The biological control, thinking about the integrated management, has been inserted with other management techniques to disease control, such as soybean anthracnose. The aims of this work were to verify the action of Trichoderma and Bacillus isolates in the induction of soybean resistance mechanisms to anthracnose as a function of seed treatment. The statistical design was entirely randomised, in a 5 x 2 (agent species x sampling times) factorial scheme with five replicates. Soybean seeds were treated with Bacillus amyloliquefaciens BV03, B. subtilis BV02, Trichoderma asperellum BV10, Carbendazim + Thiram and distilled water (control). Seven days after seedling emergence, 2 µL of 1 x 10-4 Colletotrichum truncatum spores were inoculated on the cotyledons. Catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and glyceollin (GLY) activities before and after pathogen inoculation, as well as the diameter of the anthracnose lesion on the cotyledons, were evaluated. Data were submitted to analysis of variance and, when significant, the mean values were compared by Fisher’s test (p < 0.05). The treatments did not influence the first sampling time before inoculation. Trichoderma asperellum BV10 increased POX and PAL activities up to 173%, while B. amyloliquefaciens BV03 increased POX activity. Glyceollin was not influenced by the treatments. The T. asperellum BV10 reduces the diameter of the anthracnose lesion by up to 61%. Thus, T. asperellum BV10 has the potential to control soybean anthracnose, improved the response defense against C. truncatum, when performed on seed treatment.O controle biológico, pensando no manejo integrado, tem sido inserido em conjunto com outras técnicas de manejo de doenças, como antracnose da cultura da soja. O objetivo deste trabalho foi verificar a ação de isolados de Trichoderma e Bacillus na indução de mecanismos de resistência da soja à antracnose. O delineamento estatístico foi inteiramente casualizado em esquema fatorial 5 x 2 (agentes de controle biológico x horários de coleta) com cinco repetições. Sementes de soja foram tratadas com Trichoderma asperellum BV10, Bacillus subtilis BV02, B. amyloliquefaciens BV03, Carbendazim + Thiram e água destilada (testemunha). Sete dias após a emergência foi realizada a inoculação de Colletotrichum truncatum sobre os cotilédones com 2 µL de suspensão esporos (5 x 104 mL-1) sobre os cotilédones. A atividade de catalase (CAT), peroxidase (POX), fenilalanina amônia liase (FAL), gliceolina (GLI) foram avaliadas antes e após a inoculação do patógeno. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Fisher (p<0,05). Os tratamentos não influenciaram o primeiro horário de coleta. O T. asperellum BV10 incrementou a atividade de POX e FAL em até 173%. B. amyloliquefaciens BV03 aumentou a atividade de POX. A GLI não foi influenciada pelos tratamentos em nenhum horário de coleta e ensaio. T. asperellum BV10 reduziu até 61% do diâmetro da lesão de antracnose. O tratamento de sementes de soja com T. asperellum BV10 possui potencial no controle da antracnose, aumentando a resposta de defesa de plântulas à C. truncatum.Universidade Federal Rural do Semi-Árido2022-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/969210.1590/1983-21252022v35n203rcREVISTA CAATINGA; Vol. 35 No. 2 (2022); 265-275Revista Caatinga; v. 35 n. 2 (2022); 265-2751983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/9692/10897Copyright (c) 2022 Revista Caatingainfo:eu-repo/semantics/openAccessBorges, Neilson OliveiraSolino, Antônio Jussiê da SilvaFrancischini, RicardoOliveira, Juliana Santos BatistaSchwan-Estrada, Kátia Regina FreitasCampos, Hercules Diniz2023-07-03T17:38:02Zoai:ojs.periodicos.ufersa.edu.br:article/9692Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:51.883372Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true
dc.title.none.fl_str_mv INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
INDUÇÃO DE MECANISMOS DE RESISTÊNCIA DE SOJA À ANTRACNOSE COM AGENTES DE BIOCONTROLE
title INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
spellingShingle INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
Borges, Neilson Oliveira
Colletotrichum truncatum. Glycine max. Indução de resistência.
Colletotrichum truncatum. Glycine max. Resistance induction.
title_short INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
title_full INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
title_fullStr INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
title_full_unstemmed INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
title_sort INDUCTION OF SOYBEAN RESISTANCE MECHANISMS TO ANTHRACNOSE BY BIOCONTROL AGENTS
author Borges, Neilson Oliveira
author_facet Borges, Neilson Oliveira
Solino, Antônio Jussiê da Silva
Francischini, Ricardo
Oliveira, Juliana Santos Batista
Schwan-Estrada, Kátia Regina Freitas
Campos, Hercules Diniz
author_role author
author2 Solino, Antônio Jussiê da Silva
Francischini, Ricardo
Oliveira, Juliana Santos Batista
Schwan-Estrada, Kátia Regina Freitas
Campos, Hercules Diniz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Borges, Neilson Oliveira
Solino, Antônio Jussiê da Silva
Francischini, Ricardo
Oliveira, Juliana Santos Batista
Schwan-Estrada, Kátia Regina Freitas
Campos, Hercules Diniz
dc.subject.por.fl_str_mv Colletotrichum truncatum. Glycine max. Indução de resistência.
Colletotrichum truncatum. Glycine max. Resistance induction.
topic Colletotrichum truncatum. Glycine max. Indução de resistência.
Colletotrichum truncatum. Glycine max. Resistance induction.
description The biological control, thinking about the integrated management, has been inserted with other management techniques to disease control, such as soybean anthracnose. The aims of this work were to verify the action of Trichoderma and Bacillus isolates in the induction of soybean resistance mechanisms to anthracnose as a function of seed treatment. The statistical design was entirely randomised, in a 5 x 2 (agent species x sampling times) factorial scheme with five replicates. Soybean seeds were treated with Bacillus amyloliquefaciens BV03, B. subtilis BV02, Trichoderma asperellum BV10, Carbendazim + Thiram and distilled water (control). Seven days after seedling emergence, 2 µL of 1 x 10-4 Colletotrichum truncatum spores were inoculated on the cotyledons. Catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and glyceollin (GLY) activities before and after pathogen inoculation, as well as the diameter of the anthracnose lesion on the cotyledons, were evaluated. Data were submitted to analysis of variance and, when significant, the mean values were compared by Fisher’s test (p < 0.05). The treatments did not influence the first sampling time before inoculation. Trichoderma asperellum BV10 increased POX and PAL activities up to 173%, while B. amyloliquefaciens BV03 increased POX activity. Glyceollin was not influenced by the treatments. The T. asperellum BV10 reduces the diameter of the anthracnose lesion by up to 61%. Thus, T. asperellum BV10 has the potential to control soybean anthracnose, improved the response defense against C. truncatum, when performed on seed treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/9692
10.1590/1983-21252022v35n203rc
url https://periodicos.ufersa.edu.br/caatinga/article/view/9692
identifier_str_mv 10.1590/1983-21252022v35n203rc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/9692/10897
dc.rights.driver.fl_str_mv Copyright (c) 2022 Revista Caatinga
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Revista Caatinga
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
dc.source.none.fl_str_mv REVISTA CAATINGA; Vol. 35 No. 2 (2022); 265-275
Revista Caatinga; v. 35 n. 2 (2022); 265-275
1983-2125
0100-316X
reponame:Revista Caatinga
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Revista Caatinga
collection Revista Caatinga
repository.name.fl_str_mv Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv patricio@ufersa.edu.br|| caatinga@ufersa.edu.br
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