GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO

Detalhes bibliográficos
Autor(a) principal: Welz, Vanessa Fernandes Fonseca
Data de Publicação: 2020
Outros Autores: Trettel, Jéssica Rezende, Nascimento, Andressa Bezerra, Magalhães, Hélida Mara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Caatinga
Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/8896
Resumo: Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70%  medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity.  
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spelling GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITROCRESCIMENTO, ATIVIDADE ENZIMÁTICA E ANTIOXIDANTE DE MANJERICÃO CULTIVADO IN VITROEnzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’.Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’.Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70%  medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity.  O manjericão é uma erva perene. Objetivou-se com essa pesquisa avaliar a influência da concentração do meio de cultura associado a antioxidantes e reguladores de crescimento sobre o crescimento e atividade bioquímica de plântulas de manjericão cultivadas in vitro. Sementes da cultivar genovese foram inoculadas em meio Murashige e Skoog - MS suplementado com carvão ativado e reguladores de crescimento benzilamonopurina e ácido naftalenoacético. As plântulas cresceram em condições controladas durante 80 dias e após este período avaliou-se características biométricas e bioquímicas. Maior quantidade de plântulas anormais foi verificada no meio composto por MS 100%, sacarose 30 gL-1, BAP 0,4 gL-1 e ANA 0,2 gL-1 (T4) e (T1) sem reguladores. No entanto, esse mesmo tratamento incrementou o número de folhas, e massa e fresca seca das brotações. A atividade antioxidante foi maior nas plântulas que foram mantidas no T5 - 100% MS, carvão ativado 3,0 gL-1+ 0,4 mgL-1 BAP + 0,2 mgL-1 ANA e no T3 - 70% MS, carvão ativado 3,0 gL-1+ 0,1mgL-1 BAP. A análise enzimática revelou que a enzima superóxido dismutase apresentou maior atividade em todos os tratamentos quando comparada com a catalase e a ascorbato peroxidase. Sendo assim, as plântulas de manjericão genovese que cresceram no T4 e T1 apresentaram melhores médias de crescimento em folhas e menor atividade antioxidante. Já as plântulas que cresceram no T3 apresentaram médias maiores para as enzimas catalase e ascorbato peroxidase.  Universidade Federal Rural do Semi-Árido2020-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/889610.1590/1983-21252020v33n309rcREVISTA CAATINGA; Vol. 33 No. 3 (2020); 660-670Revista Caatinga; v. 33 n. 3 (2020); 660-6701983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/8896/10278Copyright (c) 2020 Revista Caatingainfo:eu-repo/semantics/openAccessWelz, Vanessa Fernandes FonsecaTrettel, Jéssica RezendeNascimento, Andressa BezerraMagalhães, Hélida Mara2023-07-21T17:02:36Zoai:ojs.periodicos.ufersa.edu.br:article/8896Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:43.403770Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true
dc.title.none.fl_str_mv GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
CRESCIMENTO, ATIVIDADE ENZIMÁTICA E ANTIOXIDANTE DE MANJERICÃO CULTIVADO IN VITRO
title GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
spellingShingle GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
Welz, Vanessa Fernandes Fonseca
Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’.
Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’.
title_short GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
title_full GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
title_fullStr GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
title_full_unstemmed GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
title_sort GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
author Welz, Vanessa Fernandes Fonseca
author_facet Welz, Vanessa Fernandes Fonseca
Trettel, Jéssica Rezende
Nascimento, Andressa Bezerra
Magalhães, Hélida Mara
author_role author
author2 Trettel, Jéssica Rezende
Nascimento, Andressa Bezerra
Magalhães, Hélida Mara
author2_role author
author
author
dc.contributor.author.fl_str_mv Welz, Vanessa Fernandes Fonseca
Trettel, Jéssica Rezende
Nascimento, Andressa Bezerra
Magalhães, Hélida Mara
dc.subject.por.fl_str_mv Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’.
Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’.
topic Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’.
Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’.
description Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70%  medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity.  
publishDate 2020
dc.date.none.fl_str_mv 2020-07-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/8896
10.1590/1983-21252020v33n309rc
url https://periodicos.ufersa.edu.br/caatinga/article/view/8896
identifier_str_mv 10.1590/1983-21252020v33n309rc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/8896/10278
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista Caatinga
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista Caatinga
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
dc.source.none.fl_str_mv REVISTA CAATINGA; Vol. 33 No. 3 (2020); 660-670
Revista Caatinga; v. 33 n. 3 (2020); 660-670
1983-2125
0100-316X
reponame:Revista Caatinga
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
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reponame_str Revista Caatinga
collection Revista Caatinga
repository.name.fl_str_mv Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv patricio@ufersa.edu.br|| caatinga@ufersa.edu.br
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