GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/8896 |
Resumo: | Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70% medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity. |
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GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITROCRESCIMENTO, ATIVIDADE ENZIMÁTICA E ANTIOXIDANTE DE MANJERICÃO CULTIVADO IN VITROEnzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’.Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’.Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70% medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity. O manjericão é uma erva perene. Objetivou-se com essa pesquisa avaliar a influência da concentração do meio de cultura associado a antioxidantes e reguladores de crescimento sobre o crescimento e atividade bioquímica de plântulas de manjericão cultivadas in vitro. Sementes da cultivar genovese foram inoculadas em meio Murashige e Skoog - MS suplementado com carvão ativado e reguladores de crescimento benzilamonopurina e ácido naftalenoacético. As plântulas cresceram em condições controladas durante 80 dias e após este período avaliou-se características biométricas e bioquímicas. Maior quantidade de plântulas anormais foi verificada no meio composto por MS 100%, sacarose 30 gL-1, BAP 0,4 gL-1 e ANA 0,2 gL-1 (T4) e (T1) sem reguladores. No entanto, esse mesmo tratamento incrementou o número de folhas, e massa e fresca seca das brotações. A atividade antioxidante foi maior nas plântulas que foram mantidas no T5 - 100% MS, carvão ativado 3,0 gL-1+ 0,4 mgL-1 BAP + 0,2 mgL-1 ANA e no T3 - 70% MS, carvão ativado 3,0 gL-1+ 0,1mgL-1 BAP. A análise enzimática revelou que a enzima superóxido dismutase apresentou maior atividade em todos os tratamentos quando comparada com a catalase e a ascorbato peroxidase. Sendo assim, as plântulas de manjericão genovese que cresceram no T4 e T1 apresentaram melhores médias de crescimento em folhas e menor atividade antioxidante. Já as plântulas que cresceram no T3 apresentaram médias maiores para as enzimas catalase e ascorbato peroxidase. Universidade Federal Rural do Semi-Árido2020-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/889610.1590/1983-21252020v33n309rcREVISTA CAATINGA; Vol. 33 No. 3 (2020); 660-670Revista Caatinga; v. 33 n. 3 (2020); 660-6701983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/8896/10278Copyright (c) 2020 Revista Caatingainfo:eu-repo/semantics/openAccessWelz, Vanessa Fernandes FonsecaTrettel, Jéssica RezendeNascimento, Andressa BezerraMagalhães, Hélida Mara2023-07-21T17:02:36Zoai:ojs.periodicos.ufersa.edu.br:article/8896Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:43.403770Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO CRESCIMENTO, ATIVIDADE ENZIMÁTICA E ANTIOXIDANTE DE MANJERICÃO CULTIVADO IN VITRO |
title |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
spellingShingle |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO Welz, Vanessa Fernandes Fonseca Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’. Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’. |
title_short |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
title_full |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
title_fullStr |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
title_full_unstemmed |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
title_sort |
GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO |
author |
Welz, Vanessa Fernandes Fonseca |
author_facet |
Welz, Vanessa Fernandes Fonseca Trettel, Jéssica Rezende Nascimento, Andressa Bezerra Magalhães, Hélida Mara |
author_role |
author |
author2 |
Trettel, Jéssica Rezende Nascimento, Andressa Bezerra Magalhães, Hélida Mara |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Welz, Vanessa Fernandes Fonseca Trettel, Jéssica Rezende Nascimento, Andressa Bezerra Magalhães, Hélida Mara |
dc.subject.por.fl_str_mv |
Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’. Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’. |
topic |
Enzimas. Lamiaceae. Microporpagação. Ocimum basilicum L. ‘Genovese’. Enzymes. Lamiaceae. Micropropagation. Ocimum basilicum L. ‘Genovese’. |
description |
Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6-benzylaminopurine and α-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L−1 sucrose, 0.4 g L−1 6-benzylaminopurine, and 0.2 g L−1 α-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L−1 activated carbon + 0.4 mg L−1 6-benzylaminopurine + 0.2 mg L−1 α-naphthaleneacetic acid (T5) and that composed of 70% medium + 3.0 g L−1 activated carbon + 0.1 mg L−1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/8896 10.1590/1983-21252020v33n309rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/8896 |
identifier_str_mv |
10.1590/1983-21252020v33n309rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/8896/10278 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 33 No. 3 (2020); 660-670 Revista Caatinga; v. 33 n. 3 (2020); 660-670 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674028254625792 |