Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões

Detalhes bibliográficos
Autor(a) principal: Pontes, Felipe Moura
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
Texto Completo: https://repositorio.ufersa.edu.br/handle/tede/737
Resumo: Fruit flesh firmness has a close relation with the cell wall compounds, thus, a higher knowledge about the metabolism of such compounds is indispensable to aspects related to flesh texture change. In the case of melon, the study of flesh firmness is facilitated since it has a great variability, whose amplitude allows a better observation of differences between biochemistry phenomena of cell wall. For that reason, the present work aimed to evaluate the comportment of the varieties acidulus (access 16), momordica (access 2), inodorus (cv. Iracema) and cantalupensis (cv. Olympic), related to maturation and storage of the fruits. Therefore, two experiments were installed. At first one, about maturation, fruits were obtained from determined harvest times by flower anthesis, and a completely randomized design for each mentioned variety was set. In the second one, melons of acidulus, momordica and inodorus varieties were stored in cooler with humidity control (9±1 °C e 85±5%), and evaluated from fruit samples randomLy picked, on a determined time according to each variety durability. For both experiments, fruits were evaluated to physical and chemical characteristics; to the pectinases activity of pectin methylestarese, poligalacturonase and betagalactosidase; and pectin content in three solubilization levels: water soluble, chelate soluble and sodium carbonate soluble. The adequate harvest day for each melon was, 35 days after anthesis for cv. Iracema, 30 days for cv. Olympic, 30 days for access 16 and 20 days for access 2. During maturation, it was observed high flesh firmness of access 16, when compared to remain fruits evaluated, due to its low betagalactosidase activity, as well as its upkeep of chelate and sodium carbonate pectin. Access 2 showed a high decrease in firmness, followed by the tissue cracking at the end of maturation; such an event was consecutive of the water soluble pectin increase and decrease of the chelate and sodium carbonate soluble pectins, with a raise of all pectinases at the maturation ending. Access 16 and the yellow melon (cv. Iracema) has storage potential of 30 days, in refrigerated storage. Access 2, at same conditions, had durability of 10 days. The firmness loss of all studied melons types has been associated to the Betagalactosidase enzyme activity, and to the reduction of the chelate and sodium carbonate pectin fractions. Access 16 had high conservation, keeping flesh firmness until 32 days, with high pectin levels due to low pectinase levels. The access 2 fruits showed a high decrease on flesh firmness, what deteriorated the inner appearance of the fruit. Both pectinases activities as pectin dissolution contributed to the occurred
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spelling Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melõesMaturation, storage and cell wall metabolism of different melon varietiesAcidulusMomordicaFirmezaConservaçãoPectinasesCucumis meloAcidulusMomordicaFirmnessConservationPectinaseCucumis meloCIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAFruit flesh firmness has a close relation with the cell wall compounds, thus, a higher knowledge about the metabolism of such compounds is indispensable to aspects related to flesh texture change. In the case of melon, the study of flesh firmness is facilitated since it has a great variability, whose amplitude allows a better observation of differences between biochemistry phenomena of cell wall. For that reason, the present work aimed to evaluate the comportment of the varieties acidulus (access 16), momordica (access 2), inodorus (cv. Iracema) and cantalupensis (cv. Olympic), related to maturation and storage of the fruits. Therefore, two experiments were installed. At first one, about maturation, fruits were obtained from determined harvest times by flower anthesis, and a completely randomized design for each mentioned variety was set. In the second one, melons of acidulus, momordica and inodorus varieties were stored in cooler with humidity control (9±1 °C e 85±5%), and evaluated from fruit samples randomLy picked, on a determined time according to each variety durability. For both experiments, fruits were evaluated to physical and chemical characteristics; to the pectinases activity of pectin methylestarese, poligalacturonase and betagalactosidase; and pectin content in three solubilization levels: water soluble, chelate soluble and sodium carbonate soluble. The adequate harvest day for each melon was, 35 days after anthesis for cv. Iracema, 30 days for cv. Olympic, 30 days for access 16 and 20 days for access 2. During maturation, it was observed high flesh firmness of access 16, when compared to remain fruits evaluated, due to its low betagalactosidase activity, as well as its upkeep of chelate and sodium carbonate pectin. Access 2 showed a high decrease in firmness, followed by the tissue cracking at the end of maturation; such an event was consecutive of the water soluble pectin increase and decrease of the chelate and sodium carbonate soluble pectins, with a raise of all pectinases at the maturation ending. Access 16 and the yellow melon (cv. Iracema) has storage potential of 30 days, in refrigerated storage. Access 2, at same conditions, had durability of 10 days. The firmness loss of all studied melons types has been associated to the Betagalactosidase enzyme activity, and to the reduction of the chelate and sodium carbonate pectin fractions. Access 16 had high conservation, keeping flesh firmness until 32 days, with high pectin levels due to low pectinase levels. The access 2 fruits showed a high decrease on flesh firmness, what deteriorated the inner appearance of the fruit. Both pectinases activities as pectin dissolution contributed to the occurred2017-07-03A firmeza da polpa tem estreita relação com a manutenção da estrutura da parede celular, dessa forma, um maior conhecimento sobre o metabolismo da parede celular é indispensável para análise de aspectos relativos à alterações na textura da polpa. No caso do melão, o estudo da firmeza da polpa é facilitado, uma vez que este apresenta uma elevada variabilidade, cuja amplitude permite observar melhor as diferenças entre fenômenos bioquímicos na parede celular. Portanto, o presente trabalho teve como objetivo avaliar o comportamento das variedades acidulus (acesso 16), momordica (acesso 2), inodorus (cv. Iracema) e cantalupensis (cv. Olympic), com relação à maturação e armazenamento dos frutos. Para tanto, dois experimentos foram instalados. No primeiro, foram estudadas as transformações que ocorrem durante a maturação dos melões, onde os frutos foram colhidos em 5 estádios de maturação, que foram pré-determinados pela antese floral; tendo utilizado o delineamento inteiramente casualizado. No segundo, os melões das variedades acidulus, momordica e inodorus foram armazenados em ambiente refrigerado (9±1 °C e 85±5% de umidade relativa), por até 32 dias e avaliados em períodos determinados para cada variedade, de modo a constituir um delineamento inteiramente casualizado para cada tipo de melão. Em ambos os experimentos os frutos foram avaliados quanto às características físicas e químicas, à atividade das pectinametilesterase, poligalacturonase e betagalactosidase, e conteúdo de pectinas em três níveis de solubilização: solúveis em água, em quelato e carbonato de sódio. O ponto de colheita ideal para os melões foram de 35 dias após a antese para cv. Iracema, 30 dias para cv. Olympic, 30 dias para o acesso 16, e 20 dias para o acesso 2. Durante a maturação foi observada elevada firmeza da polpa do acesso 16, em relação aos demais frutos avaliados, que foi associada à uma baixa atividade da betagalactosidase, bem como a manutenção de elevadas concentrações de pectinas solúveis em quelato e carbonato de sódio. O acesso 2 apresentou uma elevada queda na firmeza, com rompimento do tecido do fruto ao final da maturação; tal acontecimento foi acompanhado da elevação da concentração de pectina solúvel em água e redução das concentrações de pectinas solúveis em quelato e carbonato de sódio, em conjunto com uma elevação da atividade das pectinases ao final da maturação. O acesso 16 e o melão amarelo (cv. Iracema) possuem potencial de armazenamento de 30 dias, em condições refrigeradas. O acesso 2, nas mesmas condições, teve vida útil pós-colheita de 10 dias. A perda da firmeza dos melões estudados está associada à atividade da enzima Beta-gal, e à redução das frações de pectina, solúveis em quelato e carbonato. O acesso 16 teve elevada vida útil, com manutenção da firmeza da polpa até 32 dias, mantendo elevados níveis de pectina, solúveis em quelato e carbonato de sódio, devido à baixa atividade das pectinases. Os frutos do acesso 2 apresentaram uma queda elevada na firmeza da polpa, fato que deteriorou a aparência interna do fruto. Tanto a atividade das pectinases quanto a dissolução das pectinas contribuíram para o ocorridoCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal Rural do Semi-ÁridoBrasilUFERSAPrograma de Pós-Graduação em FitotecniaNunes, Glauber Henrique de Sousa8288888445http://lattes.cnpq.br/6199153570472953Morais, Patrícia Lígia Dantas de9698420142http://lattes.cnpq.br/001524633448865382155097204http://lattes.cnpq.br/0352338717028438Menezes, Josivan Barbosa3568603047http://lattes.cnpq.br/9553871594940016Araújo, Francisca Marta Machado Casado de46627626349http://lattes.cnpq.br/3950159182378869Rocha, Railene Herica Carlos00820591440http://lattes.cnpq.br/0087513816949221Albuquerque, Cynthia Cavalcanti de76722376400http://lattes.cnpq.br/0424387651987567Pontes, Felipe Moura2017-07-03T14:27:44Z2017-02-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfPONTES, Felipe Moura. Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões. 2017. 88 f. Tese (Doutorado em Fitotecnia) - Universidade Federal Rural do Semi-Árido, Mossoró, 2017.https://repositorio.ufersa.edu.br/handle/tede/737porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-12-14T23:04:58Zoai:repositorio.ufersa.edu.br:tede/737Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-12-14T23:04:58Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false
dc.title.none.fl_str_mv Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
Maturation, storage and cell wall metabolism of different melon varieties
title Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
spellingShingle Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
Pontes, Felipe Moura
Acidulus
Momordica
Firmeza
Conservação
Pectinases
Cucumis melo
Acidulus
Momordica
Firmness
Conservation
Pectinase
Cucumis melo
CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
title_short Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
title_full Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
title_fullStr Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
title_full_unstemmed Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
title_sort Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões
author Pontes, Felipe Moura
author_facet Pontes, Felipe Moura
author_role author
dc.contributor.none.fl_str_mv Nunes, Glauber Henrique de Sousa
8288888445
http://lattes.cnpq.br/6199153570472953
Morais, Patrícia Lígia Dantas de
9698420142
http://lattes.cnpq.br/0015246334488653
82155097204
http://lattes.cnpq.br/0352338717028438
Menezes, Josivan Barbosa
3568603047
http://lattes.cnpq.br/9553871594940016
Araújo, Francisca Marta Machado Casado de
46627626349
http://lattes.cnpq.br/3950159182378869
Rocha, Railene Herica Carlos
00820591440
http://lattes.cnpq.br/0087513816949221
Albuquerque, Cynthia Cavalcanti de
76722376400
http://lattes.cnpq.br/0424387651987567
dc.contributor.author.fl_str_mv Pontes, Felipe Moura
dc.subject.por.fl_str_mv Acidulus
Momordica
Firmeza
Conservação
Pectinases
Cucumis melo
Acidulus
Momordica
Firmness
Conservation
Pectinase
Cucumis melo
CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
topic Acidulus
Momordica
Firmeza
Conservação
Pectinases
Cucumis melo
Acidulus
Momordica
Firmness
Conservation
Pectinase
Cucumis melo
CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA
description Fruit flesh firmness has a close relation with the cell wall compounds, thus, a higher knowledge about the metabolism of such compounds is indispensable to aspects related to flesh texture change. In the case of melon, the study of flesh firmness is facilitated since it has a great variability, whose amplitude allows a better observation of differences between biochemistry phenomena of cell wall. For that reason, the present work aimed to evaluate the comportment of the varieties acidulus (access 16), momordica (access 2), inodorus (cv. Iracema) and cantalupensis (cv. Olympic), related to maturation and storage of the fruits. Therefore, two experiments were installed. At first one, about maturation, fruits were obtained from determined harvest times by flower anthesis, and a completely randomized design for each mentioned variety was set. In the second one, melons of acidulus, momordica and inodorus varieties were stored in cooler with humidity control (9±1 °C e 85±5%), and evaluated from fruit samples randomLy picked, on a determined time according to each variety durability. For both experiments, fruits were evaluated to physical and chemical characteristics; to the pectinases activity of pectin methylestarese, poligalacturonase and betagalactosidase; and pectin content in three solubilization levels: water soluble, chelate soluble and sodium carbonate soluble. The adequate harvest day for each melon was, 35 days after anthesis for cv. Iracema, 30 days for cv. Olympic, 30 days for access 16 and 20 days for access 2. During maturation, it was observed high flesh firmness of access 16, when compared to remain fruits evaluated, due to its low betagalactosidase activity, as well as its upkeep of chelate and sodium carbonate pectin. Access 2 showed a high decrease in firmness, followed by the tissue cracking at the end of maturation; such an event was consecutive of the water soluble pectin increase and decrease of the chelate and sodium carbonate soluble pectins, with a raise of all pectinases at the maturation ending. Access 16 and the yellow melon (cv. Iracema) has storage potential of 30 days, in refrigerated storage. Access 2, at same conditions, had durability of 10 days. The firmness loss of all studied melons types has been associated to the Betagalactosidase enzyme activity, and to the reduction of the chelate and sodium carbonate pectin fractions. Access 16 had high conservation, keeping flesh firmness until 32 days, with high pectin levels due to low pectinase levels. The access 2 fruits showed a high decrease on flesh firmness, what deteriorated the inner appearance of the fruit. Both pectinases activities as pectin dissolution contributed to the occurred
publishDate 2017
dc.date.none.fl_str_mv 2017-07-03T14:27:44Z
2017-02-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PONTES, Felipe Moura. Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões. 2017. 88 f. Tese (Doutorado em Fitotecnia) - Universidade Federal Rural do Semi-Árido, Mossoró, 2017.
https://repositorio.ufersa.edu.br/handle/tede/737
identifier_str_mv PONTES, Felipe Moura. Maturação, armazenamento e metabolismo da parede celular de diferentes variedades de melões. 2017. 88 f. Tese (Doutorado em Fitotecnia) - Universidade Federal Rural do Semi-Árido, Mossoró, 2017.
url https://repositorio.ufersa.edu.br/handle/tede/737
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv CC-BY-SA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC-BY-SA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
Brasil
UFERSA
Programa de Pós-Graduação em Fitotecnia
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
Brasil
UFERSA
Programa de Pós-Graduação em Fitotecnia
dc.source.none.fl_str_mv reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
collection Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
repository.name.fl_str_mv Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br
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