Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/368 |
Resumo: | Conventional tests that evaluate sperm quality do not have the ability to measure the potential of a fertilizing sample. This study aimed to determine the ability of the collared peccary of sperm in interacting with swine oocytes recovered from antral follicles, perivitelline membrane binding capacity of the chicken egg, and finally identify relationships between sperm parameters and in vitro interaction tests. Thus study was divided in two experiments with 11 male collared peccaries from the Multiplication Center of Wild Animals (CEMAS/UFERSA). In all experiments, the semen was collected by electroejaculation and evaluated for motility, vigour, viability, normal morphology, membrane integrity, and kinetic motility parameters by CASA. In the first experiment, semen samples were analyzed by an in vitro interaction test with swine oocytes conducted under a humidified atmosphere containing 5% CO2 for 18 h at 38.5ºC. Thus, it was found that in all oocytes there interactions with the presence of spermatozoa (mean 100.00 ± 0.0), observed the mean of 19.85 ± 5.5 oocytes penetrated by spermatozoa, number bound spermatozoa 39.4 ± 4.6 and penetrated spermatozoa 2.46 ± 0.7. Also, verified a significant association between the number of bound sperm and straightness (STR) (R2=61.7%; P=0.04). In the second experiment, semen samples were frozen in a Tris-based extender plus egg yolk (10%), Aloe vera (10%) and glycerol (6%). After thawing, samples were analyzed by an egg perivitelline membrane biding test conducted under a humidified atmosphere containing 5% CO2 for 20 min at 38.5ºC. As result, we identify 140.6 ± 19.4 sperm bound to the perivitelline membrane to fresh samples (binding range 33.9 308,7), while in frozen-thawed samples, a total of 125.1 ± 5.5 sperm were found (binding range 49.8 250.7) (P>0.05). Significant associations between frozen/thawed sperm bound to perivitelline membrane and various sperm parameters as vigor (54.6%), amplitude lateral head (R=55.1%), beat cross frequency (R=76.8%), straightness (R=61.8%) and linearity (R=62.2%) were found. In conclusion, that the two tests can be used for the functional evaluation of peccaries sperm. The perivitelline membrane binding test is a reliable, practical and relatively simple method that does not require expensive equipment and can be efficiently incorporated into the routine analysis of peccaries semen samples |
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Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogosIn vitro interactions between collared peccaries (Pecari tajacu Linnaeus, 1758) spermatozoa and heterologous substratesWildlifePerivitelline membraneIn Vitro interaction testAnimais SilvestresMembrana perivitelinaTestes de interação In VitroCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAConventional tests that evaluate sperm quality do not have the ability to measure the potential of a fertilizing sample. This study aimed to determine the ability of the collared peccary of sperm in interacting with swine oocytes recovered from antral follicles, perivitelline membrane binding capacity of the chicken egg, and finally identify relationships between sperm parameters and in vitro interaction tests. Thus study was divided in two experiments with 11 male collared peccaries from the Multiplication Center of Wild Animals (CEMAS/UFERSA). In all experiments, the semen was collected by electroejaculation and evaluated for motility, vigour, viability, normal morphology, membrane integrity, and kinetic motility parameters by CASA. In the first experiment, semen samples were analyzed by an in vitro interaction test with swine oocytes conducted under a humidified atmosphere containing 5% CO2 for 18 h at 38.5ºC. Thus, it was found that in all oocytes there interactions with the presence of spermatozoa (mean 100.00 ± 0.0), observed the mean of 19.85 ± 5.5 oocytes penetrated by spermatozoa, number bound spermatozoa 39.4 ± 4.6 and penetrated spermatozoa 2.46 ± 0.7. Also, verified a significant association between the number of bound sperm and straightness (STR) (R2=61.7%; P=0.04). In the second experiment, semen samples were frozen in a Tris-based extender plus egg yolk (10%), Aloe vera (10%) and glycerol (6%). After thawing, samples were analyzed by an egg perivitelline membrane biding test conducted under a humidified atmosphere containing 5% CO2 for 20 min at 38.5ºC. As result, we identify 140.6 ± 19.4 sperm bound to the perivitelline membrane to fresh samples (binding range 33.9 308,7), while in frozen-thawed samples, a total of 125.1 ± 5.5 sperm were found (binding range 49.8 250.7) (P>0.05). Significant associations between frozen/thawed sperm bound to perivitelline membrane and various sperm parameters as vigor (54.6%), amplitude lateral head (R=55.1%), beat cross frequency (R=76.8%), straightness (R=61.8%) and linearity (R=62.2%) were found. In conclusion, that the two tests can be used for the functional evaluation of peccaries sperm. The perivitelline membrane binding test is a reliable, practical and relatively simple method that does not require expensive equipment and can be efficiently incorporated into the routine analysis of peccaries semen samplesOs testes convencionais, que avaliam a qualidade seminal, não possuem a capacidade de predizer o potencial fecundante de uma amostra. Com o intuito de superar esse entrave, objetivou-se determinar a capacidade dos espermatozoides de catetos em promover interações com oócitos suínos recuperados de folículos antrais, bem como a capacidade de ligação à membrana perivitelina do ovo de galinha e identificação das relações dos mesmos com os parâmetros espermáticos. Para tanto, o estudo foi dividido em dois experimentos, sendo utilizados 11 catetos machos, oriundos do Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Em todos os experimentos, o sêmen foi colhido por eletroejaculação e avaliado quanto à motilidade espermática, vigor, viabilidade, morfologia, parâmetros cinéticos por análise computadorizada (CASA), integridade e funcionalidade da membrana espermática. No primeiro experimento, as amostras de sêmen fresco foram analisadas pelo teste de interação in vitro com oócitos suínos em 5% CO2 por 18 h a 38,5ºC. Assim, verificou-se que em todos os oócitos avaliados existia a presença de interações com espermatozoides (média de 100 ± 0,0), tendo sido observado uma média de 19,85 ± 5,5 de oócitos com espermatozoides penetrados, 39,4 ± 4,6 espermatozoides ligados/oócito e 2,46 ± 0,7 espermatozoides penetrados/oócito. Ainda, observou-se uma significativa associação entre o número de espermatozoides ligados com índice de progressão (R=61,7%). No segundo experimento, o sêmen foi criopreservado em diluente a base de Tris, suplementado com gema de ovo (10%), Aloe vera (10%) e glicerol (3%). Após a descongelação, as amostras foram analisadas pelo teste de ligação à membrana perivitelina da gema do ovo de galinha, que foi realizado em atmosfera controlada contendo 5% CO2 por 20 min a 38,5ºC. As amostras de sêmen fresco apresentaram 140,6 ± 19,4 espermatozoides ligados a membrana perivitelina (taxa de variação de 33,9 308,7), já no sêmen congelado/descongelado, verificaram-se 125,1 ± 5,5 espermatozoides ligados (taxa de variação de 49,8 250,07) (P>0,05). Em adição, observou-se a existência de relações entre o teste de ligação e os parâmetros espermáticos: vigor (54,6%), deslocamento lateral da cabeça (R=55,1%), frequência de batimento cruzado (R=76,8%), índice de progressão (R=61,8%) e índice de linearidade (R=62,2%). Diante disso, conclui-se que ambos os testes podem ser utilizados para a avaliação funcional dos espermatozoides de catetos. Ainda, o teste de ligação à membrana perivitelina é um método confiável, prático e relativamente simples e que pode ser eficientemente incorporado à análise de rotina de amostras de sêmen catetosCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalPereira, Alexsandra FernandesCPF:91307198368http://lattes.cnpq.br/8114638410593492Silva, Alexandre RodriguesCPF:70298354387http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707684Y3Freitas, Vicente José de FigueirêdoCPF:20287020363http://lattes.cnpq.br/8123478315205308Campos, Lívia Batista2016-08-15T20:31:24Z2015-04-172015-02-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfCAMPOS, Lívia Batista. In vitro interactions between collared peccaries (Pecari tajacu Linnaeus, 1758) spermatozoa and heterologous substrates. 2015. 86 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.https://repositorio.ufersa.edu.br/handle/tede/368porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-14T00:54:10Zoai:repositorio.ufersa.edu.br:tede/368Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-14T00:54:10Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos In vitro interactions between collared peccaries (Pecari tajacu Linnaeus, 1758) spermatozoa and heterologous substrates |
title |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
spellingShingle |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos Campos, Lívia Batista Wildlife Perivitelline membrane In Vitro interaction test Animais Silvestres Membrana perivitelina Testes de interação In Vitro CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
title_full |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
title_fullStr |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
title_full_unstemmed |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
title_sort |
Interação in vitro entre espermatozoides de catetos (Pecari tajacu Linnaeus, 1758) e substratos heterólogos |
author |
Campos, Lívia Batista |
author_facet |
Campos, Lívia Batista |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pereira, Alexsandra Fernandes CPF:91307198368 http://lattes.cnpq.br/8114638410593492 Silva, Alexandre Rodrigues CPF:70298354387 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707684Y3 Freitas, Vicente José de Figueirêdo CPF:20287020363 http://lattes.cnpq.br/8123478315205308 |
dc.contributor.author.fl_str_mv |
Campos, Lívia Batista |
dc.subject.por.fl_str_mv |
Wildlife Perivitelline membrane In Vitro interaction test Animais Silvestres Membrana perivitelina Testes de interação In Vitro CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Wildlife Perivitelline membrane In Vitro interaction test Animais Silvestres Membrana perivitelina Testes de interação In Vitro CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Conventional tests that evaluate sperm quality do not have the ability to measure the potential of a fertilizing sample. This study aimed to determine the ability of the collared peccary of sperm in interacting with swine oocytes recovered from antral follicles, perivitelline membrane binding capacity of the chicken egg, and finally identify relationships between sperm parameters and in vitro interaction tests. Thus study was divided in two experiments with 11 male collared peccaries from the Multiplication Center of Wild Animals (CEMAS/UFERSA). In all experiments, the semen was collected by electroejaculation and evaluated for motility, vigour, viability, normal morphology, membrane integrity, and kinetic motility parameters by CASA. In the first experiment, semen samples were analyzed by an in vitro interaction test with swine oocytes conducted under a humidified atmosphere containing 5% CO2 for 18 h at 38.5ºC. Thus, it was found that in all oocytes there interactions with the presence of spermatozoa (mean 100.00 ± 0.0), observed the mean of 19.85 ± 5.5 oocytes penetrated by spermatozoa, number bound spermatozoa 39.4 ± 4.6 and penetrated spermatozoa 2.46 ± 0.7. Also, verified a significant association between the number of bound sperm and straightness (STR) (R2=61.7%; P=0.04). In the second experiment, semen samples were frozen in a Tris-based extender plus egg yolk (10%), Aloe vera (10%) and glycerol (6%). After thawing, samples were analyzed by an egg perivitelline membrane biding test conducted under a humidified atmosphere containing 5% CO2 for 20 min at 38.5ºC. As result, we identify 140.6 ± 19.4 sperm bound to the perivitelline membrane to fresh samples (binding range 33.9 308,7), while in frozen-thawed samples, a total of 125.1 ± 5.5 sperm were found (binding range 49.8 250.7) (P>0.05). Significant associations between frozen/thawed sperm bound to perivitelline membrane and various sperm parameters as vigor (54.6%), amplitude lateral head (R=55.1%), beat cross frequency (R=76.8%), straightness (R=61.8%) and linearity (R=62.2%) were found. In conclusion, that the two tests can be used for the functional evaluation of peccaries sperm. The perivitelline membrane binding test is a reliable, practical and relatively simple method that does not require expensive equipment and can be efficiently incorporated into the routine analysis of peccaries semen samples |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-17 2015-02-24 2016-08-15T20:31:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CAMPOS, Lívia Batista. In vitro interactions between collared peccaries (Pecari tajacu Linnaeus, 1758) spermatozoa and heterologous substrates. 2015. 86 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. https://repositorio.ufersa.edu.br/handle/tede/368 |
identifier_str_mv |
CAMPOS, Lívia Batista. In vitro interactions between collared peccaries (Pecari tajacu Linnaeus, 1758) spermatozoa and heterologous substrates. 2015. 86 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
url |
https://repositorio.ufersa.edu.br/handle/tede/368 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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1809747467463294976 |