Avaliação da transfusão sanguínea em caprinos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://doi.org/10.21708/bdtd.ppgca.tese.684 https://repositorio.ufersa.edu.br/handle/tede/684 |
Resumo: | This work aimed to evaluate the clinical, hematological, biochemical, hemogasometric, oxidative stress, lipid peroxidation and transfusion reactions of goats submitted to homologous transfusion of fresh whole blood or stored for 15 and 35 days. Eighteen healthy adult male goats were submitted to the induction of anemia and six hours later were transfused with whole blood stored in CPDA-1 bags (Citrate, phosphate, dextrose and adenine) according to the composite experimental group For 6 animals each: G0 (received fresh blood); G15 and G35 (received blood stored for 15 and 35 days, respectively). The transfusion reactions were recorded during the course of the transfusion, whereas clinical, hematological, biochemical, hemogasometric, oxidative stress and lipid peroxidation parameters were assessed by blood sampling at the following moments: before induction of anemia (TC0); 6 hours after phlebotomy and before transfusion (CT scan); 1, 6, 12, 24 and 96 hours after transfusion (T1, T6, T12, T24 and T96, respectively); 8, 16 and 32 days after transfusion (T8d, T16d and T32d, respectively). Samples of blood were also withdrawn from the pockets, prior to transfusion, for the same analyzes. The pockets of blood stored for 15 and 35 days presented more biochemical changes, hemogasometric, oxidative stress and lipid peroxidation than fresh blood bags. However, the hematological parameters were similar to each other. All groups presented transfusion reactions, among which fasciculations and vocalization occurred more frequently. In the hematological analysis, the animals that received fresh blood presented higher globular volume, number of red blood cells and hemoglobin concentration when compared to the animals that received stored blood. As for the biochemical analysis, after the transfusion it was observed an increase of the proteins in the 3 groups; Transient increase of serum glucose in G0 and G35; Elevation of total bilirubin, direct bilirubin, and urea in G15 and G35; In addition, there was elevation of creatine kinase in the 3 groups. The changes observed in the hemogasometric analysis had no clinical significance, as they were within the reference values for the species. As for oxidative stress, the animals that received stored blood presented disorder in their antioxidant system demonstrated by the alteration of the activity of SOD until the T12. In the analysis of lipid peroxidation there was no difference between the groups for the concentration of malondialdehyde. Thus, it can be concluded that transfusion of fresh total homologous blood was more efficient in restoring blood volume, number of erythrocytes and hemoglobin concentration than the transfusion of stored whole blood. However, transfusion of fresh or stored whole blood did not compromise the blood gas, lipid peroxidation, hepatic and renal functions of goats and proved to be efficient in restoring, among other components, the total proteins. Thus, blood transfusion, as performed in this study, was shown to be safe for use in the clinical practice of this species |
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Avaliação da transfusão sanguínea em caprinosEvaluation of blood transfusion in goatsHemoterapiaBodesEstresse oxidativoHemogasometriaReações transfusionaisHemotherapyGoatsOxidative stressHemogasometryTransfusion reactionsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThis work aimed to evaluate the clinical, hematological, biochemical, hemogasometric, oxidative stress, lipid peroxidation and transfusion reactions of goats submitted to homologous transfusion of fresh whole blood or stored for 15 and 35 days. Eighteen healthy adult male goats were submitted to the induction of anemia and six hours later were transfused with whole blood stored in CPDA-1 bags (Citrate, phosphate, dextrose and adenine) according to the composite experimental group For 6 animals each: G0 (received fresh blood); G15 and G35 (received blood stored for 15 and 35 days, respectively). The transfusion reactions were recorded during the course of the transfusion, whereas clinical, hematological, biochemical, hemogasometric, oxidative stress and lipid peroxidation parameters were assessed by blood sampling at the following moments: before induction of anemia (TC0); 6 hours after phlebotomy and before transfusion (CT scan); 1, 6, 12, 24 and 96 hours after transfusion (T1, T6, T12, T24 and T96, respectively); 8, 16 and 32 days after transfusion (T8d, T16d and T32d, respectively). Samples of blood were also withdrawn from the pockets, prior to transfusion, for the same analyzes. The pockets of blood stored for 15 and 35 days presented more biochemical changes, hemogasometric, oxidative stress and lipid peroxidation than fresh blood bags. However, the hematological parameters were similar to each other. All groups presented transfusion reactions, among which fasciculations and vocalization occurred more frequently. In the hematological analysis, the animals that received fresh blood presented higher globular volume, number of red blood cells and hemoglobin concentration when compared to the animals that received stored blood. As for the biochemical analysis, after the transfusion it was observed an increase of the proteins in the 3 groups; Transient increase of serum glucose in G0 and G35; Elevation of total bilirubin, direct bilirubin, and urea in G15 and G35; In addition, there was elevation of creatine kinase in the 3 groups. The changes observed in the hemogasometric analysis had no clinical significance, as they were within the reference values for the species. As for oxidative stress, the animals that received stored blood presented disorder in their antioxidant system demonstrated by the alteration of the activity of SOD until the T12. In the analysis of lipid peroxidation there was no difference between the groups for the concentration of malondialdehyde. Thus, it can be concluded that transfusion of fresh total homologous blood was more efficient in restoring blood volume, number of erythrocytes and hemoglobin concentration than the transfusion of stored whole blood. However, transfusion of fresh or stored whole blood did not compromise the blood gas, lipid peroxidation, hepatic and renal functions of goats and proved to be efficient in restoring, among other components, the total proteins. Thus, blood transfusion, as performed in this study, was shown to be safe for use in the clinical practice of this species2017-05-03Este trabalho teve como objetivo avaliar as respostas clínica, hematológica, bioquímica, hemogasométrica, estresse oxidativo, peroxidação lipídica e as reações transfusionais de caprinos submetidos à transfusão homóloga de sangue total fresco ou armazenado por 15 e 35 dias. Foram utilizados 18 caprinos machos, adultos, hígidos, os quais foram submetidos à indução de anemia e, seis horas após, transfundidos com sangue total armazenado em bolsas CPDA-1 (Citrato, fosfato, dextrose e adenina) de acordo com o grupo experimental composto por 6 animais cada: G0 (recebeu sangue fresco); G15 e G35 (receberam sangue armazenado por 15 e 35 dias, respectivamente). As reações transfusionais foram registradas durante o decorrer da transfusão, enquanto que parâmetros clínicos, hematológicos, bioquímicos, hemogasométricos, estresse oxidativo e peroxidação lipídica foram avaliados através da coleta de amostras de sangue nos seguintes momentos: antes da indução da anemia (TC0); 6 horas após a flebotomia e antes de transfusão (TC1); 1, 6, 12, 24 e 96 horas após a transfusão (T1, T6, T12, T24 e T96, respectivamente); 8, 16 e 32 dias após a transfusão (T8d, T16d e T32d, respectivamente). Amostras de sangue também foram retiradas das bolsas, antes da transfusão, para realização das mesmas análises. As bolsas de sangue armazenado por 15 e 35 dias apresentaram mais alterações bioquímicas, hemogasométricas, estresse oxidativo e peroxidação lipídica do que as bolsas de sangue fresco, porém quanto aos parâmetros hematológicos elas foram semelhantes entre si. Todos os grupos apresentaram reações transfusionais, entre as quais fasciculações e vocalização ocorreram com maior frequência. Na análise hematológica, os animais que receberam sangue fresco apresentaram maior volume globular, número de hemácias e concentração de hemoglobina quando comparados aos animais que receberam sangue armazenado. Quanto à análise bioquímica, após a transfusão observou-se aumento das proteínas nos 3 grupos; aumento transitório da glicose sérica no G0 e G35; elevação da bilirrubina total, bilirrubina direta e uréia no G15 e G35; além disso, houve elevação da creatina quinase nos 3 grupos. As alterações observadas na análise hemogasométrica não tiveram significado clínico, pois estavam dentro dos valores de referência para a espécie. Quanto ao estresse oxidativo, os animais que receberam sangue armazenado apresentaram desordem no seu sistema antioxidante demonstrada pela alteração da atividade da SOD até o T12. Na análise da peroxidação lipídica não houve diferença entre os grupos para a concentração de malondialdéido. Sendo assim, pode-se concluir que a transfusão de sangue homólogo total fresco foi mais eficiente em repor a volemia, número de hemácias e concentração de hemoglobina do que a transfusão de sangue total armazenado. Porém, a transfusão seja de sangue total fresco ou armazenado não comprometeu a gasometria, peroxidação lipídica, funções hepática e renal dos caprinos e mostrou-se eficiente em repor entre outros componentes, as proteínas totais. Sendo assim, a transfusão sanguínea, conforme realizada neste estudo, mostrou-se segura para ser utilizada na prática clínica desta espécieFundação de Apoio à Pesquisa do RNUniversidade Federal Rural do Semi-ÁridoBrasilUFERSAPrograma de Pós-Graduação em Ciência AnimalBarrêto Júnior, Raimundo Alves43214363387http://lattes.cnpq.br/051697123283849401358998426http://lattes.cnpq.br/7537160271956210Macedo, Michelly Fernandes de01208223429http://lattes.cnpq.br/3820982476384228Costa, Wirton Peixoto02866148479http://lattes.cnpq.br/3442520902027224Antunes, João Marcelo Azevedo de Paula08711888725http://lattes.cnpq.br/4718683077685105Nogueira, Andressa Francisca Silva22077893818http://lattes.cnpq.br/4705014856970093Fonseca, Nayanna Brunna da Silva2017-05-04T16:05:14Z2016-10-31info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfFONSECA, Nayanna Brunna da Silva. Avaliação da transfusão sanguínea em caprinos. 2016. 72 f. Tese (Doutorado) - Curso de Pós-graduação em Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016.https://doi.org/10.21708/bdtd.ppgca.tese.684https://repositorio.ufersa.edu.br/handle/tede/684porinfo:eu-repo/semantics/openAccessCC-BY-SAreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2024-11-21T22:10:17Zoai:repositorio.ufersa.edu.br:tede/684Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2024-11-21T22:10:17Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Avaliação da transfusão sanguínea em caprinos Evaluation of blood transfusion in goats |
title |
Avaliação da transfusão sanguínea em caprinos |
spellingShingle |
Avaliação da transfusão sanguínea em caprinos Fonseca, Nayanna Brunna da Silva Hemoterapia Bodes Estresse oxidativo Hemogasometria Reações transfusionais Hemotherapy Goats Oxidative stress Hemogasometry Transfusion reactions CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação da transfusão sanguínea em caprinos |
title_full |
Avaliação da transfusão sanguínea em caprinos |
title_fullStr |
Avaliação da transfusão sanguínea em caprinos |
title_full_unstemmed |
Avaliação da transfusão sanguínea em caprinos |
title_sort |
Avaliação da transfusão sanguínea em caprinos |
author |
Fonseca, Nayanna Brunna da Silva |
author_facet |
Fonseca, Nayanna Brunna da Silva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Barrêto Júnior, Raimundo Alves 43214363387 http://lattes.cnpq.br/0516971232838494 01358998426 http://lattes.cnpq.br/7537160271956210 Macedo, Michelly Fernandes de 01208223429 http://lattes.cnpq.br/3820982476384228 Costa, Wirton Peixoto 02866148479 http://lattes.cnpq.br/3442520902027224 Antunes, João Marcelo Azevedo de Paula 08711888725 http://lattes.cnpq.br/4718683077685105 Nogueira, Andressa Francisca Silva 22077893818 http://lattes.cnpq.br/4705014856970093 |
dc.contributor.author.fl_str_mv |
Fonseca, Nayanna Brunna da Silva |
dc.subject.por.fl_str_mv |
Hemoterapia Bodes Estresse oxidativo Hemogasometria Reações transfusionais Hemotherapy Goats Oxidative stress Hemogasometry Transfusion reactions CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Hemoterapia Bodes Estresse oxidativo Hemogasometria Reações transfusionais Hemotherapy Goats Oxidative stress Hemogasometry Transfusion reactions CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This work aimed to evaluate the clinical, hematological, biochemical, hemogasometric, oxidative stress, lipid peroxidation and transfusion reactions of goats submitted to homologous transfusion of fresh whole blood or stored for 15 and 35 days. Eighteen healthy adult male goats were submitted to the induction of anemia and six hours later were transfused with whole blood stored in CPDA-1 bags (Citrate, phosphate, dextrose and adenine) according to the composite experimental group For 6 animals each: G0 (received fresh blood); G15 and G35 (received blood stored for 15 and 35 days, respectively). The transfusion reactions were recorded during the course of the transfusion, whereas clinical, hematological, biochemical, hemogasometric, oxidative stress and lipid peroxidation parameters were assessed by blood sampling at the following moments: before induction of anemia (TC0); 6 hours after phlebotomy and before transfusion (CT scan); 1, 6, 12, 24 and 96 hours after transfusion (T1, T6, T12, T24 and T96, respectively); 8, 16 and 32 days after transfusion (T8d, T16d and T32d, respectively). Samples of blood were also withdrawn from the pockets, prior to transfusion, for the same analyzes. The pockets of blood stored for 15 and 35 days presented more biochemical changes, hemogasometric, oxidative stress and lipid peroxidation than fresh blood bags. However, the hematological parameters were similar to each other. All groups presented transfusion reactions, among which fasciculations and vocalization occurred more frequently. In the hematological analysis, the animals that received fresh blood presented higher globular volume, number of red blood cells and hemoglobin concentration when compared to the animals that received stored blood. As for the biochemical analysis, after the transfusion it was observed an increase of the proteins in the 3 groups; Transient increase of serum glucose in G0 and G35; Elevation of total bilirubin, direct bilirubin, and urea in G15 and G35; In addition, there was elevation of creatine kinase in the 3 groups. The changes observed in the hemogasometric analysis had no clinical significance, as they were within the reference values for the species. As for oxidative stress, the animals that received stored blood presented disorder in their antioxidant system demonstrated by the alteration of the activity of SOD until the T12. In the analysis of lipid peroxidation there was no difference between the groups for the concentration of malondialdehyde. Thus, it can be concluded that transfusion of fresh total homologous blood was more efficient in restoring blood volume, number of erythrocytes and hemoglobin concentration than the transfusion of stored whole blood. However, transfusion of fresh or stored whole blood did not compromise the blood gas, lipid peroxidation, hepatic and renal functions of goats and proved to be efficient in restoring, among other components, the total proteins. Thus, blood transfusion, as performed in this study, was shown to be safe for use in the clinical practice of this species |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-31 2017-05-04T16:05:14Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FONSECA, Nayanna Brunna da Silva. Avaliação da transfusão sanguínea em caprinos. 2016. 72 f. Tese (Doutorado) - Curso de Pós-graduação em Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. https://doi.org/10.21708/bdtd.ppgca.tese.684 https://repositorio.ufersa.edu.br/handle/tede/684 |
identifier_str_mv |
FONSECA, Nayanna Brunna da Silva. Avaliação da transfusão sanguínea em caprinos. 2016. 72 f. Tese (Doutorado) - Curso de Pós-graduação em Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. |
url |
https://doi.org/10.21708/bdtd.ppgca.tese.684 https://repositorio.ufersa.edu.br/handle/tede/684 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess CC-BY-SA |
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openAccess |
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CC-BY-SA |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Ciência Animal |
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reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
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Universidade Federal Rural do Semi-Árido (UFERSA) |
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UFERSA |
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UFERSA |
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Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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