Isolamento e seleção de bactérias lipolíticas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/599 |
Resumo: | The production of enzymes by microorganisms is advantageous that the vegetable and animal origin due to the ease in obtaining and handling them. The processes catalyzed by microbial enzymes are generally faster, more efficient, less costly and environmentally sustainable than those that are made with chemical catalysts. This fact directly influences the increased use of these enzymes in industrial environment. Lipases are enzymes that catalyze the hydrolysis of long chain triglyceride transforming them into glycerides and fatty acids. Microbial lipases are part of one of the most important groups of enzymes for industrial application. Besides being involved in certain processes in the food, pharmaceutical, cosmetics, textile, paper and leather, these enzymes are also used in supplementation of animal feed that have deficiency in processing lipids. On the promising potential of these enzymes, this work had as main objective the isolation of bacteria producing lipase from samples from hot springs with temperatures ranging from 37-54 ° C. The most promising lines for lipase production were selected by testing the Rhodamine B and p-nitrophenyl palmitate (pNPP). Of the 51 isolated bacterial strains, 17 demonstrated the potential lipolytic Rhodamine test. Of these, nine were selected in the enzyme activity test (pNPP) verifying that the cultivation temperature influences the production of enzymes. The temperature of 30 ° C was the most favorable to growth and production of the enzymes. For the enzymatic activity temperature, it was found that one of the lines has greater activity at 70 ° C, thereby increasing interest in the use of same in the industrial environment the thermostable enzyme production. The results reflect the importance of studying the biotechnological potential of the microbiota associated with thermal environments for prospecting producing strains of lipases, and future agro-industrial applications |
id |
UFER_95c8975d1667481e8f2fcaf3c365e9ef |
---|---|
oai_identifier_str |
oai:repositorio.ufersa.edu.br:tede/599 |
network_acronym_str |
UFER |
network_name_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository_id_str |
|
spelling |
Isolamento e seleção de bactérias lipolíticasIsolation and selection of lipolytic bacteriaEnzimaLipaseMicrorganismosBactériaEnzymeLipaseMicroorganismsBacteriaCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe production of enzymes by microorganisms is advantageous that the vegetable and animal origin due to the ease in obtaining and handling them. The processes catalyzed by microbial enzymes are generally faster, more efficient, less costly and environmentally sustainable than those that are made with chemical catalysts. This fact directly influences the increased use of these enzymes in industrial environment. Lipases are enzymes that catalyze the hydrolysis of long chain triglyceride transforming them into glycerides and fatty acids. Microbial lipases are part of one of the most important groups of enzymes for industrial application. Besides being involved in certain processes in the food, pharmaceutical, cosmetics, textile, paper and leather, these enzymes are also used in supplementation of animal feed that have deficiency in processing lipids. On the promising potential of these enzymes, this work had as main objective the isolation of bacteria producing lipase from samples from hot springs with temperatures ranging from 37-54 ° C. The most promising lines for lipase production were selected by testing the Rhodamine B and p-nitrophenyl palmitate (pNPP). Of the 51 isolated bacterial strains, 17 demonstrated the potential lipolytic Rhodamine test. Of these, nine were selected in the enzyme activity test (pNPP) verifying that the cultivation temperature influences the production of enzymes. The temperature of 30 ° C was the most favorable to growth and production of the enzymes. For the enzymatic activity temperature, it was found that one of the lines has greater activity at 70 ° C, thereby increasing interest in the use of same in the industrial environment the thermostable enzyme production. The results reflect the importance of studying the biotechnological potential of the microbiota associated with thermal environments for prospecting producing strains of lipases, and future agro-industrial applications2017-01-11A produção de enzimas por microrganismos é mais vantajosa que as de origem vegetal e animal devido à maior facilidade na obtenção e manipulação dos mesmos. Os processos catalisados por enzimas microbianas são geralmente mais rápidos, mais eficientes, de menor custo e ambientalmente sustentáveis do que aqueles que são realizados com catalisadores químicos. Esse fato influencia diretamente no aumento da utilização dessas enzimas no ambiente industrial. As lipases são enzimas que catalisam a hidrólise de longas cadeias de triglicerídeos transformando-os em glicerídeos e ácidos graxos. As lipases microbianas fazem parte de um dos mais importantes grupos de enzimas para a aplicação industrial. Além de estar envolvida em determinados processos dos setores alimentício, farmacêutico, cosméticos, têxtil, de papel e couro, essas enzimas também são utilizadas na suplementação de ração para animais que possuem deficiência no processamento de lipídeos. Diante do potencial promissor destas enzimas, este trabalho teve como objetivo principal o isolamento de bactérias produtoras de lipase a partir de amostras provenientes de águas termais com temperaturas variando entre 37 a 54 °C. As linhagens mais promissoras para produção de lipase foram selecionadas por meio do teste da Rodamina B e do p-nitrofenil palmitato (pNPP). Das 51 linhagens bacterianas isoladas, 17 demonstraram potencial lipolítico no teste de Rodamina. Destas, nove foram selecionadas no teste de atividade enzimática (pNPP) verificando que a temperatura de cultivo influencia na produção das enzimas. A temperatura de 30 °C foi a mais favorável para crescimento e produção das enzimas. Quanto à temperatura de atividade enzimática, verificou-se que uma das linhagens possui maior atividade à 70 °C, aumentando assim, o interesse da utilização da mesma no meio industrial pela produção enzimática termoestável. Os resultados obtidos refletem a importância em se estudar o potencial biotecnológico da microbiota associada aos ambientes térmicos para a prospecção de linhagens produtoras de lipases, e suas futuras aplicações agroindustriaisCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal Rural do Semi-ÁridoBrasilUFERSAPrograma de Pós-Graduação em Produção AnimalMatias, Fernanda97250180010http://lattes.cnpq.br/3668017453612079Cordeiro, Luiz Augusto Vieira12741719896http://lattes.cnpq.br/701753283405703909374853493Rocha, Lidianne Leal96282509300http://lattes.cnpq.br/6236113002989510Barreto, Raphaela Vasconcelos Gomes79687130300http://lattes.cnpq.br/4617213714835536Souza, Camila Michele Pereira de2017-03-21T14:54:53Z2016-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSOUZA, Carla Michele Pereira de. Isolamento e seleção de bactérias lipolíticas. 2016. 67 f. Dissertação (Mestrado) - Curso de Pós-graduação em Produção Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016.https://repositorio.ufersa.edu.br/handle/tede/599porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-14T02:19:42Zoai:repositorio.ufersa.edu.br:tede/599Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-14T02:19:42Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Isolamento e seleção de bactérias lipolíticas Isolation and selection of lipolytic bacteria |
title |
Isolamento e seleção de bactérias lipolíticas |
spellingShingle |
Isolamento e seleção de bactérias lipolíticas Souza, Camila Michele Pereira de Enzima Lipase Microrganismos Bactéria Enzyme Lipase Microorganisms Bacteria CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Isolamento e seleção de bactérias lipolíticas |
title_full |
Isolamento e seleção de bactérias lipolíticas |
title_fullStr |
Isolamento e seleção de bactérias lipolíticas |
title_full_unstemmed |
Isolamento e seleção de bactérias lipolíticas |
title_sort |
Isolamento e seleção de bactérias lipolíticas |
author |
Souza, Camila Michele Pereira de |
author_facet |
Souza, Camila Michele Pereira de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Matias, Fernanda 97250180010 http://lattes.cnpq.br/3668017453612079 Cordeiro, Luiz Augusto Vieira 12741719896 http://lattes.cnpq.br/7017532834057039 09374853493 Rocha, Lidianne Leal 96282509300 http://lattes.cnpq.br/6236113002989510 Barreto, Raphaela Vasconcelos Gomes 79687130300 http://lattes.cnpq.br/4617213714835536 |
dc.contributor.author.fl_str_mv |
Souza, Camila Michele Pereira de |
dc.subject.por.fl_str_mv |
Enzima Lipase Microrganismos Bactéria Enzyme Lipase Microorganisms Bacteria CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
topic |
Enzima Lipase Microrganismos Bactéria Enzyme Lipase Microorganisms Bacteria CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
description |
The production of enzymes by microorganisms is advantageous that the vegetable and animal origin due to the ease in obtaining and handling them. The processes catalyzed by microbial enzymes are generally faster, more efficient, less costly and environmentally sustainable than those that are made with chemical catalysts. This fact directly influences the increased use of these enzymes in industrial environment. Lipases are enzymes that catalyze the hydrolysis of long chain triglyceride transforming them into glycerides and fatty acids. Microbial lipases are part of one of the most important groups of enzymes for industrial application. Besides being involved in certain processes in the food, pharmaceutical, cosmetics, textile, paper and leather, these enzymes are also used in supplementation of animal feed that have deficiency in processing lipids. On the promising potential of these enzymes, this work had as main objective the isolation of bacteria producing lipase from samples from hot springs with temperatures ranging from 37-54 ° C. The most promising lines for lipase production were selected by testing the Rhodamine B and p-nitrophenyl palmitate (pNPP). Of the 51 isolated bacterial strains, 17 demonstrated the potential lipolytic Rhodamine test. Of these, nine were selected in the enzyme activity test (pNPP) verifying that the cultivation temperature influences the production of enzymes. The temperature of 30 ° C was the most favorable to growth and production of the enzymes. For the enzymatic activity temperature, it was found that one of the lines has greater activity at 70 ° C, thereby increasing interest in the use of same in the industrial environment the thermostable enzyme production. The results reflect the importance of studying the biotechnological potential of the microbiota associated with thermal environments for prospecting producing strains of lipases, and future agro-industrial applications |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-25 2017-03-21T14:54:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUZA, Carla Michele Pereira de. Isolamento e seleção de bactérias lipolíticas. 2016. 67 f. Dissertação (Mestrado) - Curso de Pós-graduação em Produção Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. https://repositorio.ufersa.edu.br/handle/tede/599 |
identifier_str_mv |
SOUZA, Carla Michele Pereira de. Isolamento e seleção de bactérias lipolíticas. 2016. 67 f. Dissertação (Mestrado) - Curso de Pós-graduação em Produção Animal, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. |
url |
https://repositorio.ufersa.edu.br/handle/tede/599 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Produção Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Produção Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
_version_ |
1809747468035817472 |