Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFERSA |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/315 |
Resumo: | Gossypol is a polyphenol compound produced by the pigment glands of cotton plants (Gossypium spp.) and presents proven deleterious action on spermatogenesis, rodent´s estrous cycle, granulosa cells steroidogenesis in pigs, early termination of pregnancy and embryonic development. However, the effects on sheep ovaries were not yet studied. This study was divided into two stages and aimed to determine in vitro and in vivo effect of gossypol on sheep ovarian follicles. In the first stage, eight ovarian fragments from each ewe were cultured in α-MEM supplemented culture and subjected to three concentrations of gossypol-acetic acid (5, 10 and 20 µg/mL) for periods of 24 hours and seven days. In vitro action of gossypol resulted in a significant reduction in the number of viable ovarian follicles of all development stages, and a significant increase in the number of atretic follicles after 24 hours and seven days of cultivation (p < 0,01). In the latter it was remarkable the absence of viable antral follicles and the primordial follicles presented less than 50% of viability. In the second stage, we used twelve crossbred ewes, which were randomly divided into two groups and fed diets containing cottonseed cake or not (control group). Concentrate feed was offered at a rate of 1.5% of body weight of the animal for 63 days. Concentrations of total and free gossypol in cottonseed cake was 3.28 mg/g and 0.11 mg/g, respectively. Throughout the trial period no animal showed clinical signs of toxicosis. There was no interference on progesterone concentrations, weight, weight gain and ovaries measurements. Only occasional changes on vaginal cytology, hematological and biochemical parameters were noted. However, treatment with gossypol was responsible for significant reduction in the number of viable ovarian follicles (20.6% in treated sheep and 65.1% the control group) and significant increase in the number of atretic follicles (79.4% in treated sheep and 34.9% in control group), interfering at all stages of follicular development (p < 0,01). In conclusion, the gossypol promotes direct damage to the ovarian follicles of sheep, resulting in an increase on the proportion of atretic follicles and reduction of viable follicles both in vitro and in vivo |
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Blanco, Benito Sotohttp://lattes.cnpq.br/5009042939501223Silva, José Augusto Bastos Afonso dahttp://lattes.cnpq.br/9754109726295756Braga, Alexandre Paulahttp://lattes.cnpq.br/1480331510087793Sousa, Marcos Antonio Nobrega dehttp://lattes.cnpq.br/4412109117021331Borges, Jose Renato Junqueirahttp://lattes.cnpq.br/3586382943482522http://lattes.cnpq.br/1614037096605288Câmara, Antônio Carlos Lopes2016-08-15T20:27:59Z2016-05-312015-04-16CÂMARA, Antônio Carlos Lopes. Determination of gossypol toxicity on sheep ovarian foliccles. 2015. 82 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.https://repositorio.ufersa.edu.br/handle/tede/315Gossypol is a polyphenol compound produced by the pigment glands of cotton plants (Gossypium spp.) and presents proven deleterious action on spermatogenesis, rodent´s estrous cycle, granulosa cells steroidogenesis in pigs, early termination of pregnancy and embryonic development. However, the effects on sheep ovaries were not yet studied. This study was divided into two stages and aimed to determine in vitro and in vivo effect of gossypol on sheep ovarian follicles. In the first stage, eight ovarian fragments from each ewe were cultured in α-MEM supplemented culture and subjected to three concentrations of gossypol-acetic acid (5, 10 and 20 µg/mL) for periods of 24 hours and seven days. In vitro action of gossypol resulted in a significant reduction in the number of viable ovarian follicles of all development stages, and a significant increase in the number of atretic follicles after 24 hours and seven days of cultivation (p < 0,01). In the latter it was remarkable the absence of viable antral follicles and the primordial follicles presented less than 50% of viability. In the second stage, we used twelve crossbred ewes, which were randomly divided into two groups and fed diets containing cottonseed cake or not (control group). Concentrate feed was offered at a rate of 1.5% of body weight of the animal for 63 days. Concentrations of total and free gossypol in cottonseed cake was 3.28 mg/g and 0.11 mg/g, respectively. Throughout the trial period no animal showed clinical signs of toxicosis. There was no interference on progesterone concentrations, weight, weight gain and ovaries measurements. Only occasional changes on vaginal cytology, hematological and biochemical parameters were noted. However, treatment with gossypol was responsible for significant reduction in the number of viable ovarian follicles (20.6% in treated sheep and 65.1% the control group) and significant increase in the number of atretic follicles (79.4% in treated sheep and 34.9% in control group), interfering at all stages of follicular development (p < 0,01). In conclusion, the gossypol promotes direct damage to the ovarian follicles of sheep, resulting in an increase on the proportion of atretic follicles and reduction of viable follicles both in vitro and in vivoO gossipol é um composto polifenólico produzido pelas glândulas de pigmento do algodoeiro (Gossypium spp.); apresentando ação deletéria comprovada sobre a espermatogênese, ciclo estral de roedores, esteroidogênese nas células da granulosa em suínos, interrupção precoce da gestação e desenvolvimento embrionário inicial. No entanto, os efeitos sobre os ovários de ovelhas ainda não havia sido estudado. O presente estudo foi dividido em duas etapas: determinar o efeito in vitro e in vivo do gossipol sobre folículos ovarianos de ovelhas. Na primeira etapa, oito fragmentos ovarianos de cada animal foram cultivados em Meio α-MEM suplementado e submetidas a cultivo em três concentrações de gossipol-ácido acético (5, 10 e 20 μg/mL) por períodos de 24h e sete dias. A ação do gossipol in vitro proporcionou redução significativa no número de folículos viáveis em todos os estágios de desenvolvimento folicular; e aumento significativo no número de folículos atrésicos após 24h e sete dias de cultivo (p < 0,01). Neste último também foi notório a ausência de folículos antrais viáveis, e, os folículos primordiais apresentaram viabilidade inferior a 50%. Na segunda etapa, foram utilizadas doze ovelhas mestiças, que foram aleatoriamente separadas em dois grupos e alimentadas com rações contendo torta de algodão ou não (grupo controle). A ração foi oferecida na proporção de 1,5% do peso vivo do animal por 63 dias. As concentrações de gossipol total e livre na torta de algodão foram 3,28 mg/g e 0,11 mg/g, respectivamente. Durante todo o período experimental, nenhum animal apresentou qualquer sinal clínico de intoxicação. Não houve interferência sobre as concentrações de progesterona, peso, ganho em peso e medidas ovarianas. Apenas alterações ocasionais sobre a citologia vaginal, parâmetros hematológicos e bioquímicos foram evidenciadas. Entretanto, o tratamento com gossipol foi responsável por redução significante no número de folículos ovarianos viáveis (20,6% nas ovelhas tratadas e 65,1% no grupo controle) e aumento significante no número de folículos atrésicos (79,4% nas ovelhas tratadas e 34,9% no grupo controle), com interferência em todas as fases de desenvolvimento folicular (p < 0,01). Em conclusão, o gossipol promove danos diretos aos folículos ovarianos de ovelhas, resultando em aumento na proporção de folículos atrésicos e redução dos folículos viáveis tanto in vitro como in vivoConselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfhttp://repositorio.ufersa.edu.br/retrieve/704/AntonioCLC_TESE.pdf.jpgporUniversidade Federal Rural do Semi-ÁridoPrograma de Pós-Graduação em Ciência AnimalUFERSABRCentro de Ciências Agrárias - CCACC-BY-SAinfo:eu-repo/semantics/openAccessSheepGossypium spp.Antinutriciona factorFollicles atreticCottonseed cakeOvelhasGossypium spp.Fator antinutricionaFolículos atrésicosTorta de algodãoCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADeterminação da toxicidade do gossipol em folículos ovarianos de ovelhasDetermination of gossypol toxicity on sheep ovarian folicclesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Biblioteca Digital de Teses e Dissertações da UFERSAinstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSATEXTAntonioCLC_TESE.pdf.txtAntonioCLC_TESE.pdf.txtExtracted texttext/plain154295https://repositorio.ufersa.edu.br//bitstream/tede/315/3/AntonioCLC_TESE.pdf.txt43ac3780c190e924b413178b45097c75MD53ORIGINALAntonioCLC_TESE.pdfapplication/pdf1302444https://repositorio.ufersa.edu.br//bitstream/tede/315/1/AntonioCLC_TESE.pdfe465e8b76dbdec077ba3f1534f5d9898MD51THUMBNAILAntonioCLC_TESE.pdf.jpgAntonioCLC_TESE.pdf.jpgGenerated Thumbnailimage/jpeg3290https://repositorio.ufersa.edu.br//bitstream/tede/315/2/AntonioCLC_TESE.pdf.jpg5010904fd6af009818d7308e6fe8e6edMD52tede/3152022-06-29 15:02:37.238oai:repositorio.ufersa.edu.br:tede/315Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufersa.edu.br/PUBhttp://bdtd.ufersa.edu.br/oai/requestdirecaosisbi@ufersa.edu.br|| direcaosisbi@ufersa.edu.bropendoar:2022-06-29T18:02:37Biblioteca Digital de Teses e Dissertações da UFERSA - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.por.fl_str_mv |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
dc.title.alternative.eng.fl_str_mv |
Determination of gossypol toxicity on sheep ovarian foliccles |
title |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
spellingShingle |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas Câmara, Antônio Carlos Lopes Sheep Gossypium spp. Antinutriciona factor Follicles atretic Cottonseed cake Ovelhas Gossypium spp. Fator antinutriciona Folículos atrésicos Torta de algodão CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
title_full |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
title_fullStr |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
title_full_unstemmed |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
title_sort |
Determinação da toxicidade do gossipol em folículos ovarianos de ovelhas |
author |
Câmara, Antônio Carlos Lopes |
author_facet |
Câmara, Antônio Carlos Lopes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Blanco, Benito Soto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5009042939501223 |
dc.contributor.referee1.fl_str_mv |
Silva, José Augusto Bastos Afonso da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9754109726295756 |
dc.contributor.referee2.fl_str_mv |
Braga, Alexandre Paula |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1480331510087793 |
dc.contributor.referee3.fl_str_mv |
Sousa, Marcos Antonio Nobrega de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4412109117021331 |
dc.contributor.referee4.fl_str_mv |
Borges, Jose Renato Junqueira |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/3586382943482522 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1614037096605288 |
dc.contributor.author.fl_str_mv |
Câmara, Antônio Carlos Lopes |
contributor_str_mv |
Blanco, Benito Soto Silva, José Augusto Bastos Afonso da Braga, Alexandre Paula Sousa, Marcos Antonio Nobrega de Borges, Jose Renato Junqueira |
dc.subject.eng.fl_str_mv |
Sheep Gossypium spp. Antinutriciona factor Follicles atretic Cottonseed cake |
topic |
Sheep Gossypium spp. Antinutriciona factor Follicles atretic Cottonseed cake Ovelhas Gossypium spp. Fator antinutriciona Folículos atrésicos Torta de algodão CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.por.fl_str_mv |
Ovelhas Gossypium spp. Fator antinutriciona Folículos atrésicos Torta de algodão |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Gossypol is a polyphenol compound produced by the pigment glands of cotton plants (Gossypium spp.) and presents proven deleterious action on spermatogenesis, rodent´s estrous cycle, granulosa cells steroidogenesis in pigs, early termination of pregnancy and embryonic development. However, the effects on sheep ovaries were not yet studied. This study was divided into two stages and aimed to determine in vitro and in vivo effect of gossypol on sheep ovarian follicles. In the first stage, eight ovarian fragments from each ewe were cultured in α-MEM supplemented culture and subjected to three concentrations of gossypol-acetic acid (5, 10 and 20 µg/mL) for periods of 24 hours and seven days. In vitro action of gossypol resulted in a significant reduction in the number of viable ovarian follicles of all development stages, and a significant increase in the number of atretic follicles after 24 hours and seven days of cultivation (p < 0,01). In the latter it was remarkable the absence of viable antral follicles and the primordial follicles presented less than 50% of viability. In the second stage, we used twelve crossbred ewes, which were randomly divided into two groups and fed diets containing cottonseed cake or not (control group). Concentrate feed was offered at a rate of 1.5% of body weight of the animal for 63 days. Concentrations of total and free gossypol in cottonseed cake was 3.28 mg/g and 0.11 mg/g, respectively. Throughout the trial period no animal showed clinical signs of toxicosis. There was no interference on progesterone concentrations, weight, weight gain and ovaries measurements. Only occasional changes on vaginal cytology, hematological and biochemical parameters were noted. However, treatment with gossypol was responsible for significant reduction in the number of viable ovarian follicles (20.6% in treated sheep and 65.1% the control group) and significant increase in the number of atretic follicles (79.4% in treated sheep and 34.9% in control group), interfering at all stages of follicular development (p < 0,01). In conclusion, the gossypol promotes direct damage to the ovarian follicles of sheep, resulting in an increase on the proportion of atretic follicles and reduction of viable follicles both in vitro and in vivo |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-04-16 |
dc.date.accessioned.fl_str_mv |
2016-08-15T20:27:59Z |
dc.date.available.fl_str_mv |
2016-05-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CÂMARA, Antônio Carlos Lopes. Determination of gossypol toxicity on sheep ovarian foliccles. 2015. 82 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufersa.edu.br/handle/tede/315 |
identifier_str_mv |
CÂMARA, Antônio Carlos Lopes. Determination of gossypol toxicity on sheep ovarian foliccles. 2015. 82 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
url |
https://repositorio.ufersa.edu.br/handle/tede/315 |
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por |
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por |
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Universidade Federal Rural do Semi-Árido |
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Programa de Pós-Graduação em Ciência Animal |
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UFERSA |
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BR |
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Centro de Ciências Agrárias - CCA |
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Universidade Federal Rural do Semi-Árido |
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