Análise de QTLs e herança de caracteres associados à qualidade de melão
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFERSA |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/174 |
Resumo: | The quality of the melon is one of the main objectives of breeding programs. An important aspect for the breeder is to know its inheritance as it assists in choosing the breeding strategy with time and resource savings. In addition, the use of biotechnological tools to assist in the knowledge of architecture of the characters and facilitate the selection process may be useful. The objectives of this study were: to identify SSR markers linked to QTLs of six characters related to the quality of melon and to study the genetic control of traits related to fruit quality of melon through classic study of generations involving mixed models. Based on this, we evaluated 100 F2: 3 families of crossing between the parents 'Hales Best Jumbo' (HBJ) and 'MR-1', momordica type, in the years 2013 and 2014. For the phenotypic evaluation of the families, two experiments were carried out in randomized block design with three replications. Each plot consisted of a line with five plants, spaced 2.0 m between rows and 0.3 m between plants. The traits evaluated were: fruit weight, shape index, flesh thickness, flesh firmness, soluble solids and percentage of fruit cracking. From the results, the identification of stable molecular markers linked to quality traits of melon fruit through multiple regression procedure was possible. Using multiple regression process were identified markers associated to the average fruit weight characters, shape index, flesh thickness, firmness, soluble solids and percentage of fruit cracking. The CMMS22-2, CMCTN4 and CMMS4-3 markers were the most stable in both evaluations. The CMGAN3 markers, CMAGN33, CMSTN4, CMMS4-3, CMMS22-2 were those who stood out as the most promising for assisted selection to improve the melon quality. For the inheritance study, the generations F1, F2 and backcross of crossings OL x A-16 and OL-PV were evaluated in two separate experiments carried out in randomized block design with three replications, in 2012. The traits evaluated were: average fruit weight, shape index, flesh thickness, flesh firmness and soluble solids. The analyses were performed using mixed models to estimate the parameters of the additive-dominant model and the variance components. The heritability in the narrow and broad sense and number of loci that control the character were estimated. Maximum likelihood hierarchical genetic models were used in tests of hypothesis in order to assess the presence of polygenes and major effect gene in the genetic control character. From the results, it is concluded that the inheritance of the studied characters is complex, with the presence of major gene and polygenes with additive and dominance effects |
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Nunes, Glauber Henrique de Sousahttp://lattes.cnpq.br/6199153570472953Antonio, Rafaela Priscilahttp://lattes.cnpq.br/4570230133800020Lima, Lidiane Kely dehttp://lattes.cnpq.br/2278487003313130Silveira, Lindomar Maria dahttp://lattes.cnpq.br/2988333573887017http://lattes.cnpq.br/1157538942515084Dantas, Ana Carolina de Assis2016-08-12T19:18:31Z2016-02-172015-02-20DANTAS, Ana Carolina de Assis. QTL analysis and inheritance of fruit quality traits in melon. 2015. 96 f. Tese (Doutorado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.https://repositorio.ufersa.edu.br/handle/tede/174The quality of the melon is one of the main objectives of breeding programs. An important aspect for the breeder is to know its inheritance as it assists in choosing the breeding strategy with time and resource savings. In addition, the use of biotechnological tools to assist in the knowledge of architecture of the characters and facilitate the selection process may be useful. The objectives of this study were: to identify SSR markers linked to QTLs of six characters related to the quality of melon and to study the genetic control of traits related to fruit quality of melon through classic study of generations involving mixed models. Based on this, we evaluated 100 F2: 3 families of crossing between the parents 'Hales Best Jumbo' (HBJ) and 'MR-1', momordica type, in the years 2013 and 2014. For the phenotypic evaluation of the families, two experiments were carried out in randomized block design with three replications. Each plot consisted of a line with five plants, spaced 2.0 m between rows and 0.3 m between plants. The traits evaluated were: fruit weight, shape index, flesh thickness, flesh firmness, soluble solids and percentage of fruit cracking. From the results, the identification of stable molecular markers linked to quality traits of melon fruit through multiple regression procedure was possible. Using multiple regression process were identified markers associated to the average fruit weight characters, shape index, flesh thickness, firmness, soluble solids and percentage of fruit cracking. The CMMS22-2, CMCTN4 and CMMS4-3 markers were the most stable in both evaluations. The CMGAN3 markers, CMAGN33, CMSTN4, CMMS4-3, CMMS22-2 were those who stood out as the most promising for assisted selection to improve the melon quality. For the inheritance study, the generations F1, F2 and backcross of crossings OL x A-16 and OL-PV were evaluated in two separate experiments carried out in randomized block design with three replications, in 2012. The traits evaluated were: average fruit weight, shape index, flesh thickness, flesh firmness and soluble solids. The analyses were performed using mixed models to estimate the parameters of the additive-dominant model and the variance components. The heritability in the narrow and broad sense and number of loci that control the character were estimated. Maximum likelihood hierarchical genetic models were used in tests of hypothesis in order to assess the presence of polygenes and major effect gene in the genetic control character. From the results, it is concluded that the inheritance of the studied characters is complex, with the presence of major gene and polygenes with additive and dominance effectsA qualidade dos frutos é um dos principais objetivos dos programas de melhoramento genético do meloeiro. Um aspecto importante para o melhorista é conhecer a herança do caráter, de vez que auxilia o pesquisador na escolha da estratégia de melhoramento com economia de tempo e recursos. Além disso, o uso de ferramentas biotecnológicas auxilia no conhecimento da arquitetura dos caracteres e facilita o processo seletivo. Os objetivos do presente trabalho foram: identificar marcadores SSR ligados aos locos gênicos (Quantitative Trait Loci - QTLs) controladores de seis caracteres relacionados à qualidade de frutos de meloeiro e estudar o controle genético de caracteres relacionados à qualidade de frutos de melão por meio de estudo clássico de gerações envolvendo modelos mistos. Foram avaliadas 100 famílias F2:3 do cruzamento entre os genitores Hales Best Jumbo (HBJ), tipo cantaloupensis e MR-1 , tipo momordica, nos anos de 2013 e 2014. Para a avaliação fenotípica das famílias, foram conduzidos dois experimentos, nos quais foi utilizado o delineamento em blocos casualizados com três repetições. Cada parcela foi constituída por uma linha com cinco plantas, com espaçamento de 2,0 m entre as linhas e 0,3 m entre plantas. Foram avaliadas as características peso médio do fruto, índice de formato, espessura da polpa, firmeza da polpa, sólidos solúveis e porcentagem de rachadura do fruto. Para genotipagem, foram utilizados os marcadores moleculares microssatélites ou SSR. Foi possível a identificação de marcadores moleculares estáveis ligados a caracteres de qualidade de frutos de melão pelo processo de regressão múltipla. Foram identificados marcadores associados aos caracteres peso médio do fruto, índice de formato, espessura da polpa, firmeza da polpa, sólidos solúveis e porcentagem de rachadura do fruto. Os marcadores CMMS22-2, CMCTN4 e CMMS4-3 foram os mais estáveis nas duas avaliações. Os marcadores CMGAN3, CMAGN33, CMSTN4, CMMS4-3, CMMS22-2 são os mais promissores para seleção assistida, visando ao melhoramento da qualidade de frutos do meloeiro. Para o estudo de herança, foram avaliadas os genitores: OL, tipo cantaloupensis; PV, tipo não definido; A-16, tipo acidulus; as gerações F1, F2 e os retrocruzamentos dos OL x A-16 e OL x PV em dois ensaios distintos conduzidos em blocos casualizados com três repetições no ano de 2012. Os caracteres avaliados foram: peso médio de fruto, índice de formato, espessura da polpa, firmeza da polpa e sólidos solúveis. As análises foram feitas utilizando modelos mistos para estimar os parâmetros do modelo aditivo-dominante e os componentes de variância. Foram estimados os parâmetros de herdabilidades nos sentidos amplo e restrito e número de locos que controlam o caráter. Utilizaram-se testes de hipóteses de máxima verossimilhança de modelos genéticos hierárquicos para avaliar a presença de poligenes e gene de efeito maior no controle genético do caráter. Pelos resultados, conclui-se que a herança dos caracteres estudados é complexa, com a presença de gene de efeito maior e poligenes com efeitos aditivos e de dominânciaCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfhttp://repositorio.ufersa.edu.br/retrieve/435/AnaCAD_TESE.pdf.jpgporUniversidade Federal Rural do Semi-ÁridoPrograma de Pós-Graduação em FitotecniaUFERSABRCentro de Ciências Agrárias - CCACC-BY-SAinfo:eu-repo/semantics/openAccessMelonSSR markersPoligenesQuality FruitsMajor effect genesMelãoMarcadores SSRPoligenesFrutos - qualidadeGene de efeito maiorCNPQ::CIENCIAS AGRARIAS::AGRONOMIAAnálise de QTLs e herança de caracteres associados à qualidade de melãoQTL analysis and inheritance of fruit quality traits in meloninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Biblioteca Digital de Teses e Dissertações da UFERSAinstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSATEXTAnaCAD_TESE.pdf.txtAnaCAD_TESE.pdf.txtExtracted texttext/plain195339https://repositorio.ufersa.edu.br//bitstream/tede/174/3/AnaCAD_TESE.pdf.txtb43ac206eda9f4524b2367d6378cbdd7MD53ORIGINALAnaCAD_TESE.pdfapplication/pdf1798262https://repositorio.ufersa.edu.br//bitstream/tede/174/1/AnaCAD_TESE.pdf234cf9c328c4c92a52de25fe960cac0aMD51THUMBNAILAnaCAD_TESE.pdf.jpgAnaCAD_TESE.pdf.jpgGenerated Thumbnailimage/jpeg2656https://repositorio.ufersa.edu.br//bitstream/tede/174/2/AnaCAD_TESE.pdf.jpgb7995510b02fbf9aa841df40589c781cMD52tede/1742022-07-05 16:36:19.214oai:repositorio.ufersa.edu.br:tede/174Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufersa.edu.br/PUBhttp://bdtd.ufersa.edu.br/oai/requestdirecaosisbi@ufersa.edu.br|| direcaosisbi@ufersa.edu.bropendoar:2022-07-05T19:36:19Biblioteca Digital de Teses e Dissertações da UFERSA - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.por.fl_str_mv |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
dc.title.alternative.eng.fl_str_mv |
QTL analysis and inheritance of fruit quality traits in melon |
title |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
spellingShingle |
Análise de QTLs e herança de caracteres associados à qualidade de melão Dantas, Ana Carolina de Assis Melon SSR markers Poligenes Quality Fruits Major effect genes Melão Marcadores SSR Poligenes Frutos - qualidade Gene de efeito maior CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
title_full |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
title_fullStr |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
title_full_unstemmed |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
title_sort |
Análise de QTLs e herança de caracteres associados à qualidade de melão |
author |
Dantas, Ana Carolina de Assis |
author_facet |
Dantas, Ana Carolina de Assis |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nunes, Glauber Henrique de Sousa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6199153570472953 |
dc.contributor.advisor-co1.fl_str_mv |
Antonio, Rafaela Priscila |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4570230133800020 |
dc.contributor.referee1.fl_str_mv |
Lima, Lidiane Kely de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2278487003313130 |
dc.contributor.referee2.fl_str_mv |
Silveira, Lindomar Maria da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2988333573887017 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1157538942515084 |
dc.contributor.author.fl_str_mv |
Dantas, Ana Carolina de Assis |
contributor_str_mv |
Nunes, Glauber Henrique de Sousa Antonio, Rafaela Priscila Lima, Lidiane Kely de Silveira, Lindomar Maria da |
dc.subject.eng.fl_str_mv |
Melon SSR markers Poligenes Quality Fruits Major effect genes |
topic |
Melon SSR markers Poligenes Quality Fruits Major effect genes Melão Marcadores SSR Poligenes Frutos - qualidade Gene de efeito maior CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.por.fl_str_mv |
Melão Marcadores SSR Poligenes Frutos - qualidade Gene de efeito maior |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The quality of the melon is one of the main objectives of breeding programs. An important aspect for the breeder is to know its inheritance as it assists in choosing the breeding strategy with time and resource savings. In addition, the use of biotechnological tools to assist in the knowledge of architecture of the characters and facilitate the selection process may be useful. The objectives of this study were: to identify SSR markers linked to QTLs of six characters related to the quality of melon and to study the genetic control of traits related to fruit quality of melon through classic study of generations involving mixed models. Based on this, we evaluated 100 F2: 3 families of crossing between the parents 'Hales Best Jumbo' (HBJ) and 'MR-1', momordica type, in the years 2013 and 2014. For the phenotypic evaluation of the families, two experiments were carried out in randomized block design with three replications. Each plot consisted of a line with five plants, spaced 2.0 m between rows and 0.3 m between plants. The traits evaluated were: fruit weight, shape index, flesh thickness, flesh firmness, soluble solids and percentage of fruit cracking. From the results, the identification of stable molecular markers linked to quality traits of melon fruit through multiple regression procedure was possible. Using multiple regression process were identified markers associated to the average fruit weight characters, shape index, flesh thickness, firmness, soluble solids and percentage of fruit cracking. The CMMS22-2, CMCTN4 and CMMS4-3 markers were the most stable in both evaluations. The CMGAN3 markers, CMAGN33, CMSTN4, CMMS4-3, CMMS22-2 were those who stood out as the most promising for assisted selection to improve the melon quality. For the inheritance study, the generations F1, F2 and backcross of crossings OL x A-16 and OL-PV were evaluated in two separate experiments carried out in randomized block design with three replications, in 2012. The traits evaluated were: average fruit weight, shape index, flesh thickness, flesh firmness and soluble solids. The analyses were performed using mixed models to estimate the parameters of the additive-dominant model and the variance components. The heritability in the narrow and broad sense and number of loci that control the character were estimated. Maximum likelihood hierarchical genetic models were used in tests of hypothesis in order to assess the presence of polygenes and major effect gene in the genetic control character. From the results, it is concluded that the inheritance of the studied characters is complex, with the presence of major gene and polygenes with additive and dominance effects |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-20 |
dc.date.accessioned.fl_str_mv |
2016-08-12T19:18:31Z |
dc.date.available.fl_str_mv |
2016-02-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DANTAS, Ana Carolina de Assis. QTL analysis and inheritance of fruit quality traits in melon. 2015. 96 f. Tese (Doutorado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufersa.edu.br/handle/tede/174 |
identifier_str_mv |
DANTAS, Ana Carolina de Assis. QTL analysis and inheritance of fruit quality traits in melon. 2015. 96 f. Tese (Doutorado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
url |
https://repositorio.ufersa.edu.br/handle/tede/174 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
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Programa de Pós-Graduação em Fitotecnia |
dc.publisher.initials.fl_str_mv |
UFERSA |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro de Ciências Agrárias - CCA |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
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