Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos

Detalhes bibliográficos
Autor(a) principal: Silva, Shamyra Georgia de Azevedo e
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
Texto Completo: https://repositorio.ufersa.edu.br/handle/tede/94
Resumo: Silk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genome
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spelling Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicosGenetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungiCalotropis proceraPathogenic fungigenetic variabilityCalotropis proceraFungos fitopatogênicosVariabilidade genéticaCNPQ::CIENCIAS AGRARIAS::AGRONOMIASilk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genomeFlor de Seda [Calotropis procera (Aiton) W.T. Aiton] é uma planta que tem recebido destaque por se tratar de uma espécie que possui diversas funcionalidades como alimentação animal, medicina tradicional, agricultura, meio ambiente, indústria cosmética e têxtil, podendo também atuar no combate à pragas, fungos e vírus através de mecanismos de defesa associados ao seu látex. Objetivou-se nesse estudo avaliar fluidos laticíferos da Flor de Seda na inibição do desenvolvimento de fungos fitopatogênicos de Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus e estudar a divergência genética de genótipos de Flor de Seda por meio de marcadores moleculares RAPD e ISSR. Vinte amostras de folhas da espécie foram coletadas na cidade de Grossos/RN e na cidade de Mossoró/RN no Campus Leste da UFERSA (dez plantas para cada local de coleta). O látex das plantas foram testados em quatro diferentes tipos de fungos fitopatogênicos, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus com duas diferentes metodologias (adicionando o látex ao meio de cultura antes e após a polimerização do meio) e com dois níveis diferentes de concentração (puro e diluído 1:1). Para as análises moleculares, o DNA de cada uma das plantas foi extraído com 11 primers RAPD e 10 primers ISSR. Para as análises do látex, foi observado que não houve efeito inibitório para nenhum dos isolados de Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus em nenhuma das concentrações testadas nas condições estudadas. Os resultados moleculares obtidos revelaram um bom padrão de amplificação para ambos os marcadores de DNA, mostrando à formação de grupos distintos, tendo sido detectada a presença de variabilidade genética. Para as análises com os marcadores RAPD houve a formação de quatro grupos distintos, e para o marcador ISSR foi possível observar a formação de seis grupos. Quando se analisou os dois marcadores de forma correlacionada foi possível detectar a formação de sete grupos distintos demonstrando a existência de variabilidade. A diferença encontrada nos resultados de ambos marcadores pode ser explicada por se tratar de dois marcadores que cobriram diferentes regiões do genomaCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em FitotecniaHolanda, Ioná Santos AraújoCPF:65180062500http://lattes.cnpq.br/2079609001869001Silveira, Lindomar Maria daCPF:88207340444http://lattes.cnpq.br/2988333573887017Silva, Paulo Cesar Moura daCPF:91358340497http://lattes.cnpq.br/4070582933073155Silva, Shamyra Georgia de Azevedo e2016-08-12T19:15:31Z2016-02-152015-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.https://repositorio.ufersa.edu.br/handle/tede/94porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-13T23:29:55Zoai:repositorio.ufersa.edu.br:tede/94Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-13T23:29:55Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false
dc.title.none.fl_str_mv Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi
title Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
spellingShingle Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
Silva, Shamyra Georgia de Azevedo e
Calotropis procera
Pathogenic fungi
genetic variability
Calotropis procera
Fungos fitopatogênicos
Variabilidade genética
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
title_short Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
title_full Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
title_fullStr Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
title_full_unstemmed Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
title_sort Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
author Silva, Shamyra Georgia de Azevedo e
author_facet Silva, Shamyra Georgia de Azevedo e
author_role author
dc.contributor.none.fl_str_mv Holanda, Ioná Santos Araújo
CPF:65180062500
http://lattes.cnpq.br/2079609001869001
Silveira, Lindomar Maria da
CPF:88207340444
http://lattes.cnpq.br/2988333573887017
Silva, Paulo Cesar Moura da
CPF:91358340497
http://lattes.cnpq.br/4070582933073155
dc.contributor.author.fl_str_mv Silva, Shamyra Georgia de Azevedo e
dc.subject.por.fl_str_mv Calotropis procera
Pathogenic fungi
genetic variability
Calotropis procera
Fungos fitopatogênicos
Variabilidade genética
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
topic Calotropis procera
Pathogenic fungi
genetic variability
Calotropis procera
Fungos fitopatogênicos
Variabilidade genética
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
description Silk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genome
publishDate 2015
dc.date.none.fl_str_mv 2015-02-27
2016-08-12T19:15:31Z
2016-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.
https://repositorio.ufersa.edu.br/handle/tede/94
identifier_str_mv SILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.
url https://repositorio.ufersa.edu.br/handle/tede/94
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv CC-BY-SA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC-BY-SA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
BR
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Fitotecnia
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
BR
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Fitotecnia
dc.source.none.fl_str_mv reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
collection Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
repository.name.fl_str_mv Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br
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