Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/94 |
Resumo: | Silk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genome |
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Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicosGenetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungiCalotropis proceraPathogenic fungigenetic variabilityCalotropis proceraFungos fitopatogênicosVariabilidade genéticaCIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIASilk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genomeFlor de Seda [Calotropis procera (Aiton) W.T. Aiton] é uma planta que tem recebido destaque por se tratar de uma espécie que possui diversas funcionalidades como alimentação animal, medicina tradicional, agricultura, meio ambiente, indústria cosmética e têxtil, podendo também atuar no combate à pragas, fungos e vírus através de mecanismos de defesa associados ao seu látex. Objetivou-se nesse estudo avaliar fluidos laticíferos da Flor de Seda na inibição do desenvolvimento de fungos fitopatogênicos de Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus e estudar a divergência genética de genótipos de Flor de Seda por meio de marcadores moleculares RAPD e ISSR. Vinte amostras de folhas da espécie foram coletadas na cidade de Grossos/RN e na cidade de Mossoró/RN no Campus Leste da UFERSA (dez plantas para cada local de coleta). O látex das plantas foram testados em quatro diferentes tipos de fungos fitopatogênicos, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus com duas diferentes metodologias (adicionando o látex ao meio de cultura antes e após a polimerização do meio) e com dois níveis diferentes de concentração (puro e diluído 1:1). Para as análises moleculares, o DNA de cada uma das plantas foi extraído com 11 primers RAPD e 10 primers ISSR. Para as análises do látex, foi observado que não houve efeito inibitório para nenhum dos isolados de Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae e Monosporascus cannonballus em nenhuma das concentrações testadas nas condições estudadas. Os resultados moleculares obtidos revelaram um bom padrão de amplificação para ambos os marcadores de DNA, mostrando à formação de grupos distintos, tendo sido detectada a presença de variabilidade genética. Para as análises com os marcadores RAPD houve a formação de quatro grupos distintos, e para o marcador ISSR foi possível observar a formação de seis grupos. Quando se analisou os dois marcadores de forma correlacionada foi possível detectar a formação de sete grupos distintos demonstrando a existência de variabilidade. A diferença encontrada nos resultados de ambos marcadores pode ser explicada por se tratar de dois marcadores que cobriram diferentes regiões do genomaCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em FitotecniaHolanda, Ioná Santos AraújoCPF:65180062500http://lattes.cnpq.br/2079609001869001Silveira, Lindomar Maria daCPF:88207340444http://lattes.cnpq.br/2988333573887017Silva, Paulo Cesar Moura daCPF:91358340497http://lattes.cnpq.br/4070582933073155Silva, Shamyra Georgia de Azevedo e2016-08-12T19:15:31Z2016-02-152015-02-27info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfSILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015.https://repositorio.ufersa.edu.br/handle/tede/94porinfo:eu-repo/semantics/openAccessCC-BY-SAreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-12-19T01:36:38Zoai:repositorio.ufersa.edu.br:tede/94Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-12-19T01:36:38Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi |
title |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
spellingShingle |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos Silva, Shamyra Georgia de Azevedo e Calotropis procera Pathogenic fungi genetic variability Calotropis procera Fungos fitopatogênicos Variabilidade genética CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
title_short |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
title_full |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
title_fullStr |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
title_full_unstemmed |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
title_sort |
Estudo de diversidade genética e efeito inibitório do látex de Calotropis procera em fungos fitopatogênicos |
author |
Silva, Shamyra Georgia de Azevedo e |
author_facet |
Silva, Shamyra Georgia de Azevedo e |
author_role |
author |
dc.contributor.none.fl_str_mv |
Holanda, Ioná Santos Araújo CPF:65180062500 http://lattes.cnpq.br/2079609001869001 Silveira, Lindomar Maria da CPF:88207340444 http://lattes.cnpq.br/2988333573887017 Silva, Paulo Cesar Moura da CPF:91358340497 http://lattes.cnpq.br/4070582933073155 |
dc.contributor.author.fl_str_mv |
Silva, Shamyra Georgia de Azevedo e |
dc.subject.por.fl_str_mv |
Calotropis procera Pathogenic fungi genetic variability Calotropis procera Fungos fitopatogênicos Variabilidade genética CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
topic |
Calotropis procera Pathogenic fungi genetic variability Calotropis procera Fungos fitopatogênicos Variabilidade genética CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
description |
Silk Flower [Calotropis procera (Aiton) W.T. Aiton] is a plant that has been highlighted by the case of a species that has several features such as animal feed, traditional medicine, agriculture, environment, cosmetics and textile industry, and may also act to combat pests, fungi and viruses through mechanisms defense associated with your latex. This study aimed to evaluate latex fluid from Silk Flower in inhibiting the development of pathogenic fungi of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus and study the genetic diversity from Silk Flower genotypes using the RAPD and ISSR molecular markers. Twenty samples of species of leaves were collected in the Grossos/RN and Mossoró/RN cities (ten plants from each city). The plants latex were tested on four different types of phytopathogenic fungi, Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus with two different methods (adding latex to the culture medium before and after polymerization middle), and with two different concentration level (neat and diluted 1:1). For molecular analysis, DNA of each plant was extracted with 11 RAPD primers and 10 ISSR primers. For latex analyzes, it was observed that there was no inhibitory effect for any of Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum musae and Monosporascus cannonballus isolates in any of the concentrations tested under the conditions studied. Molecular results showed a good standard for both amplification of DNA markers, showing the formation of different groups, and the presence of genetic variability has been detected. For the analysis with molecular markers there was the formation of the four distinct groups, and in the ISSR marker was observed the formation of six groups. When it was analyzed the two markers in a corresponding manner it was possible to detect the formation of seven distinct groups showing the existence of variability. The difference in the results of both markers can be explained by the case of two markers that covered different regions of the genome |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-27 2016-08-12T19:15:31Z 2016-02-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. https://repositorio.ufersa.edu.br/handle/tede/94 |
identifier_str_mv |
SILVA, Shamyra Georgia de Azevedo e. Genetic diversity study and inhibitory effect of Calotropis procera latex in pathogenic fungi. 2015. 95 f. Dissertação (Mestrado em Agricultura Tropical) - Universidade Federal Rural do Semi-Árido, Mossoró, 2015. |
url |
https://repositorio.ufersa.edu.br/handle/tede/94 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess CC-BY-SA |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
CC-BY-SA |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Fitotecnia |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Fitotecnia |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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1816711950377156608 |