Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)

Detalhes bibliográficos
Autor(a) principal: Luns, Darcy Adriann Rebonato
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/7802
Resumo: The tick is the ectoparasite that affects the cattle in the world. Their losses are caused as a result of the animal generated anemia, hindering weight gain and reducing milk production, abrasions on leather, transmission of toxins and pathogens besides spending on acaricide to control it. Two surveys were conducted to evaluate different extracts for control of cattle tick. The first experiment was to evaluate the efficacy of aqueous extracts of Cinamomo, Purple Pepper, Garlic, Herb-of-Santa-Maria and Tobacco in controlling this pest. Initially, the effects of the extracts to 15% (w / v) on the position and viability of both eggs and larvae of the tick following the methodology Drummond (1973) in addition to quantifying the total phenolic content and total flavonoid extract each. The most effective treatments to control the cattle tick were the 88.45% efficiency with smoke extract followed by the extract from the bark of the Cinamomo effectively to 63.63%. The extracts showed higher total phenolic content were the Cinamomo bark extract with 17.83 mg / g extract and the conventional Garlic with 16.79 mg / g. The extracts that showed the highest flavonoids contents were extracts of Yerba de-Santa-Maria with 5.4 mg / g and also the shell of cinamomo with 4.42 mg / g. It was observed that there is no relation between the total phenolic content and total flavonoid with insecticide activity of plant extracts studied. The tobacco and the Cinamomo showed potential as an alternative method for the control of cattle tick. The second experiment aimed to test in the extracts with higher activity ticks associated with a polysaccharide biofilm. The extracts were tested Tobacco, garlic and cinamomo at 5% concentration associated and not associated with biofilm, a combination of tobacco and cinnamon extract and 2.5% pure biofilm. Drummond's method (1973) for in vitro testing was used. The cinamomo extract associated with biofilm showed the best results demonstrating that the use of biofilm is a viable alternative to enhance the insecticide activity of the extract. The combination of cinnamon extract and tobacco statistically equal results to cinnamon extracts and tobacco insulated with double the concentration indicating a possible synergy.
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spelling Martins, Isabella Vilhena FreireQueiroz, Vagner Tebaldi deLuns, Darcy Adriann RebonatoSeveri, Juliana AparecidaNunes, Erika Takagi2018-08-01T22:56:59Z2018-08-012018-08-01T22:56:59Z2015-02-25The tick is the ectoparasite that affects the cattle in the world. Their losses are caused as a result of the animal generated anemia, hindering weight gain and reducing milk production, abrasions on leather, transmission of toxins and pathogens besides spending on acaricide to control it. Two surveys were conducted to evaluate different extracts for control of cattle tick. The first experiment was to evaluate the efficacy of aqueous extracts of Cinamomo, Purple Pepper, Garlic, Herb-of-Santa-Maria and Tobacco in controlling this pest. Initially, the effects of the extracts to 15% (w / v) on the position and viability of both eggs and larvae of the tick following the methodology Drummond (1973) in addition to quantifying the total phenolic content and total flavonoid extract each. The most effective treatments to control the cattle tick were the 88.45% efficiency with smoke extract followed by the extract from the bark of the Cinamomo effectively to 63.63%. The extracts showed higher total phenolic content were the Cinamomo bark extract with 17.83 mg / g extract and the conventional Garlic with 16.79 mg / g. The extracts that showed the highest flavonoids contents were extracts of Yerba de-Santa-Maria with 5.4 mg / g and also the shell of cinamomo with 4.42 mg / g. It was observed that there is no relation between the total phenolic content and total flavonoid with insecticide activity of plant extracts studied. The tobacco and the Cinamomo showed potential as an alternative method for the control of cattle tick. The second experiment aimed to test in the extracts with higher activity ticks associated with a polysaccharide biofilm. The extracts were tested Tobacco, garlic and cinamomo at 5% concentration associated and not associated with biofilm, a combination of tobacco and cinnamon extract and 2.5% pure biofilm. Drummond's method (1973) for in vitro testing was used. The cinamomo extract associated with biofilm showed the best results demonstrating that the use of biofilm is a viable alternative to enhance the insecticide activity of the extract. The combination of cinnamon extract and tobacco statistically equal results to cinnamon extracts and tobacco insulated with double the concentration indicating a possible synergy.O carrapato bovino tem sido responsável por grandes perdas econômicas na pecuária pela transmissão de doenças e redução da produção de carne e leite. Neste contexto, os produtos naturais constituem uma alternativa potencial aos carrapaticidas comerciais quepodem gerar acúmulo no meio ambiente e resíduos nos alimentos. Sendo assim, objetivou-se determinar o teor de fenóis totais e flavonoides totais e avaliar a eficácia carrapaticida de extratos vegetaisassociados ou não a biofilme sobre Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887). As soluções extrativas deCapsicum chinense, Nicotiana tabacum, Chenopoide ambrosioides, Melia azedarach e Allium sativumforam testadas a 15%. A eficácia carrapaticida foi avaliada pela imersão de teleóginas nas soluções extrativas segundo Drummond (1973). O teste foi realizado em triplicata com 10 carrapatos por repetição. As soluções extrativas que apresentaram a maior eficiência carrapaticida foram incorporadas a 5% em soluções filmogênicas e avaliadas no controle, in vitro, de R. microplus utilizando a metodologia supracitada.Os maiores teores de fenóis totais e flavonóides totais foram encontrados nas soluções extrativas preparadas a partir da casca de cinamomo e das folhas de erva-de-santa-maria. As soluções extrativas a 15%com maior eficácia carrapaticida foram as de fumo (89,02%), casca de cinamomo (85,33%) e alho orgânico (58,36%). O biofilme incorporado com extrato de cinamomo a 5% apresentou o maior efeito carrapaticida (76,41%) quando comparado ao biofilme associado aos extratos de fumo (58,31 %), alho (34,16 %) e biofilme não associado (60,86%). Estudos futuros são importantes para determinar o efeito residual do extrato de cinamomo associado ao biofilme para avaliar seu potencial em assegurar a liberação controlada dos princípios ativos desse extrato.Texthttp://repositorio.ufes.br/handle/10/7802porUniversidade Federal do Espírito SantoMestrado em Ciências VeterináriasPrograma de Pós-Graduação em Ciências VeterináriasUFESBRCentro de Ciências Agrárias e EngenhariasNicotiana tabacumMelia azedarachAllium sativumBiocarrapaticidaControle parasitárioExtratos vegetaisMedicina Veterinária619Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_9382_Darcy Adriann.pdfapplication/pdf1067264http://repositorio.ufes.br/bitstreams/ecf9e313-521f-4657-9ac5-bc9ebb1f53b7/download04c6250a2beb7cde0fdce121daa52a0fMD5110/78022024-06-24 08:37:50.451oai:repositorio.ufes.br:10/7802http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-07-11T14:40:07.696205Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
title Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
spellingShingle Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
Luns, Darcy Adriann Rebonato
Nicotiana tabacum
Melia azedarach
Allium sativum
Biocarrapaticida
Controle parasitário
Extratos vegetais
Medicina Veterinária
619
title_short Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
title_full Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
title_fullStr Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
title_full_unstemmed Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
title_sort Extratos vegetais associados ou não a biofilme no controle de rhipicephalus (boophilus) microplus (canestrini, 1887)
author Luns, Darcy Adriann Rebonato
author_facet Luns, Darcy Adriann Rebonato
author_role author
dc.contributor.advisor-co1.fl_str_mv Martins, Isabella Vilhena Freire
dc.contributor.advisor1.fl_str_mv Queiroz, Vagner Tebaldi de
dc.contributor.author.fl_str_mv Luns, Darcy Adriann Rebonato
dc.contributor.referee1.fl_str_mv Severi, Juliana Aparecida
dc.contributor.referee2.fl_str_mv Nunes, Erika Takagi
contributor_str_mv Martins, Isabella Vilhena Freire
Queiroz, Vagner Tebaldi de
Severi, Juliana Aparecida
Nunes, Erika Takagi
dc.subject.por.fl_str_mv Nicotiana tabacum
Melia azedarach
Allium sativum
Biocarrapaticida
Controle parasitário
Extratos vegetais
topic Nicotiana tabacum
Melia azedarach
Allium sativum
Biocarrapaticida
Controle parasitário
Extratos vegetais
Medicina Veterinária
619
dc.subject.cnpq.fl_str_mv Medicina Veterinária
dc.subject.udc.none.fl_str_mv 619
description The tick is the ectoparasite that affects the cattle in the world. Their losses are caused as a result of the animal generated anemia, hindering weight gain and reducing milk production, abrasions on leather, transmission of toxins and pathogens besides spending on acaricide to control it. Two surveys were conducted to evaluate different extracts for control of cattle tick. The first experiment was to evaluate the efficacy of aqueous extracts of Cinamomo, Purple Pepper, Garlic, Herb-of-Santa-Maria and Tobacco in controlling this pest. Initially, the effects of the extracts to 15% (w / v) on the position and viability of both eggs and larvae of the tick following the methodology Drummond (1973) in addition to quantifying the total phenolic content and total flavonoid extract each. The most effective treatments to control the cattle tick were the 88.45% efficiency with smoke extract followed by the extract from the bark of the Cinamomo effectively to 63.63%. The extracts showed higher total phenolic content were the Cinamomo bark extract with 17.83 mg / g extract and the conventional Garlic with 16.79 mg / g. The extracts that showed the highest flavonoids contents were extracts of Yerba de-Santa-Maria with 5.4 mg / g and also the shell of cinamomo with 4.42 mg / g. It was observed that there is no relation between the total phenolic content and total flavonoid with insecticide activity of plant extracts studied. The tobacco and the Cinamomo showed potential as an alternative method for the control of cattle tick. The second experiment aimed to test in the extracts with higher activity ticks associated with a polysaccharide biofilm. The extracts were tested Tobacco, garlic and cinamomo at 5% concentration associated and not associated with biofilm, a combination of tobacco and cinnamon extract and 2.5% pure biofilm. Drummond's method (1973) for in vitro testing was used. The cinamomo extract associated with biofilm showed the best results demonstrating that the use of biofilm is a viable alternative to enhance the insecticide activity of the extract. The combination of cinnamon extract and tobacco statistically equal results to cinnamon extracts and tobacco insulated with double the concentration indicating a possible synergy.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-25
dc.date.accessioned.fl_str_mv 2018-08-01T22:56:59Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T22:56:59Z
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dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Veterinárias
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Veterinárias
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências Agrárias e Engenharias
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Veterinárias
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