Qualidade química de suco de uva comercial, perfil químico de bananas e açaí juçara e atividade quimiopreventiva de câncer de extratos de bananas

• Autor(a) principal: Oliveira, Bruno Gomes de
• Data de Publicação: 2019
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/11307
• topic: Perfil químico; Espectrometria de Massas; Adulteração de suco de uva e Quimi; Química; Suco de uva - Indústria; 54

Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT ICR-MS) allied to electrospray ionization (ESI) is a powerful tool for chemical analysis. In this work, a methodology was developed for the identification and quantification of grape juice with apple juiceadulteration. Anunivariate and two multivariate calibration models (PLS and MRL) were developed. The three models presented results with good precision and accuracy. Eleven samples of commercial whole grape juice were tested. Of these eleven samples, two of them presented apple juice content of approximately 24% m/m and the other nine juices investigated they either did not contain apple juice or had a content below the limit of quantification.In addition, using the ESI(-) FT ICR-MS, it was possible to study the glycidic and phenolic profile of bananas of the Pacovan, Ambrosia, Vitória, Japira, Prata Comum and Tropical varieties at four maturation stages. It was possible to observe the increase of the carbohydrate content in the fruits, during maturation. The Prata (Japira, Vitória, PrataComum and Pacovan) bananas had a monosaccharide content higher than the disaccharide content. Conversely, Gros Michel (Ambrosia) and Maçã(Tropical) had a higher disaccharide content than the monosaccharide content. The polyphenolic profile of these samples indicated a predominance of flavonoids and cinnamic acids, of which 20 polyphenols were detected, for example: epicatechin, hexoside ferulic acid, caffeic acid derivative, gallic acid derivative, proanthocyanidin and quercetin deoxyhexosehexoside.The cancer chemopreventive potential associated with quinone reductase induction,inhibition of the nitric oxideproduction, NF-kB and aromatase were also analyzed.Pacovan cultivar, maturation stage 2, showed high inhibition of NF-kB whereas Japira cultivar, at maturation stages 2, 4 and 7 and Ambrosia, stage 4, showed aromatase inhibition higher than 50%. All samples except Pacovan at maturity stage 7, Tropical at maturation stage 7, showed inhibition of iNOS greater than 80%.The polyphenolic profile of seven genotypes ofjuçara was also investigated in this work. Polyphenols were classified into three phytochemical classes: flavonoids, phenolic acids and anthocyanins, with the most abundant flavonoid class.Among the identified compounds are dihydrokaempferol, protocatecuic hexoside acid, syringe hexoside acid, orientin and malvidin-glycoside.