Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica

Detalhes bibliográficos
Autor(a) principal: Sousa, Dyeime Ribeiro
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/7761
Resumo: The fascioliasis is a disease caused by the parasite Fasciola hepatica which can cause severe liver fibrosis. This study aimed to characterize the immunophenotype of cells involved in liver fibrogenesis of cattle with this disease. To this, 74 bovine livers condemned for fascioliasis were collected for histological processing and immunohistochemistry with anti-a-SMA, anti-elastin and anti-ER-TR7 antibodies. Was taken the classification of the degree of fibrosis followed by identification and quantification of each cell type in the parenchyma and portal space. Were used descriptive statistics and Spearman correlation test. In the right lobe of the liver 90.5% (67/74) was classified as fibrosis grade 1, 8.1% (6/74) as fibrosis grade 2 and 1.4% (01/74) as fibrosis grade 3. In the left hepatic lobe, 55.4% (41/74) of livers were classified as grade 1, 28.4% (21/74) as grade 2 and 16.2% (12/74) as grade 3. There were significant positive association (? = 0.41, p <0.0001) between the degree of fibrosis and hepatic lobe, revealing more fibrosis in the left lobe. Observed hepatic stellate cell (HSC) distributed in the parenchyma and myofibroblasts (MF) in portal spaces. No fibroblasts were found. The mean of HSC was 23.9 and there was significant correlation between the degree of fibrosis (? = -0.44, p <0.0001) and the amount of HSC (? = -0.61, p <0.0001) in the right and left lobes. The MF were found around the portal structures, venules, arterioles and biliar ducts and there was positive and significant correlation between the number of layers around each structure of the EP with the degree of fibrosis (? = 0.19, p <0.0001), though, has not been influenced by any structure of the portal space, in particular (p> 0.05). Concluded that the cells that participate in the fibrogenesis of the bovine livers with chronic fascioliasis are HSC and MF. These cells are involved in hepatic fibrogenesis because they increase in number with the progression of fibrosis
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spelling Nunes, Louisiane de CarvalhoSousa, Dyeime RibeiroHataka, AlessandreBoeloni, J.N.2018-08-01T22:56:53Z2018-08-012018-08-01T22:56:53Z2014-02-21The fascioliasis is a disease caused by the parasite Fasciola hepatica which can cause severe liver fibrosis. This study aimed to characterize the immunophenotype of cells involved in liver fibrogenesis of cattle with this disease. To this, 74 bovine livers condemned for fascioliasis were collected for histological processing and immunohistochemistry with anti-a-SMA, anti-elastin and anti-ER-TR7 antibodies. Was taken the classification of the degree of fibrosis followed by identification and quantification of each cell type in the parenchyma and portal space. Were used descriptive statistics and Spearman correlation test. In the right lobe of the liver 90.5% (67/74) was classified as fibrosis grade 1, 8.1% (6/74) as fibrosis grade 2 and 1.4% (01/74) as fibrosis grade 3. In the left hepatic lobe, 55.4% (41/74) of livers were classified as grade 1, 28.4% (21/74) as grade 2 and 16.2% (12/74) as grade 3. There were significant positive association (? = 0.41, p <0.0001) between the degree of fibrosis and hepatic lobe, revealing more fibrosis in the left lobe. Observed hepatic stellate cell (HSC) distributed in the parenchyma and myofibroblasts (MF) in portal spaces. No fibroblasts were found. The mean of HSC was 23.9 and there was significant correlation between the degree of fibrosis (? = -0.44, p <0.0001) and the amount of HSC (? = -0.61, p <0.0001) in the right and left lobes. The MF were found around the portal structures, venules, arterioles and biliar ducts and there was positive and significant correlation between the number of layers around each structure of the EP with the degree of fibrosis (? = 0.19, p <0.0001), though, has not been influenced by any structure of the portal space, in particular (p> 0.05). Concluded that the cells that participate in the fibrogenesis of the bovine livers with chronic fascioliasis are HSC and MF. These cells are involved in hepatic fibrogenesis because they increase in number with the progression of fibrosisA fasciolose é uma doença causada por um trematódeo Fasciola sp. que pode ocasionar fibrose hepática severa. Objetivou-se caracterizar o imunofenótipo das células envolvidas na fibrogênese hepática de bovinos com esta enfermidade. Para isto, 74 fígados bovinos condenados por fasciolose foram coletados para processamento histológico e imunoistoquímico com os anticorpos anti-α-SMA, anti-elastina e anti-ER-TR7. Foi feita a classificação do grau de fibrose seguida pela identificação e quantificação de cada tipo celular no parênquima e nos espaços porta (EP). A análise estatística foi realizada pelo método descritivo e pelo teste de correlação de Spearman. No lobo hepático direito 90,5% (67/74) dos fígados foram classificados como fibrose de grau 1; 8,1% (6/74) como fibrose de grau 2 e 1,4% (01/74) como fibrose de grau 3. No lobo hepático esquerdo, 55,4% (41/74) dos fígados foram classificados como grau 1; 28,4% (21/74) como grau 2 e 16,2% (12/74) como grau 3. Houve associação positiva e significativa (ρ=0,41; p<0,0001) entre o grau de fibrose e o lobo hepático, revelando mais fibrose no lobo esquerdo. Observaram-se as células estreladas hepáticas (CEH) distribuídas no parênquima e miofibroblastos (MF) nos EP. Não foram encontrados fibroblastos. A média de CEH foi de 23,9 e houve correlação significativa entre os graus de fibrose (ρ=-0,44, p<0,0001) e a quantidade de CEH (ρ=-0,61, p<0,0001) nos lobos hepáticos, direito e esquerdo. Os miofibroblastos (MF) foram encontrados em torno das estruturas portais, vênula, arteríola e ducto biliar e houve correlação positiva e significativa do número de camadas em volta de cada estrutura do EP com o grau de fibrose (ρ=0,19, p<0,0001), embora, não tenha sido observada influência de qualquer estrutura do EP, em particular, sobre o grau de fibrose hepática (p>0,05). Conclui-se que as células CEH e os MF participam da fibrogênese de fígados bovinos com fasciolose crônica.Texthttp://repositorio.ufes.br/handle/10/7761porUniversidade Federal do Espírito SantoMestrado em Ciências VeterináriasPrograma de Pós-Graduação em Ciências VeterináriasUFESBRCentro de Ciências Agrárias e EngenhariasFascioloseFibrogêneseImunoistoquímicaBovino - DoençasFasciola hepáticaCiências AgráriasCaracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepaticainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_7521_Dyeime Ribeiro de Souza20150312-73655.pdfapplication/pdf2758326http://repositorio.ufes.br/bitstreams/eefc36d8-185f-4892-9c95-995f749dc58c/downloada243531277c9fb368a4c1d7ca96c451fMD5110/77612024-06-24 08:37:47.884oai:repositorio.ufes.br:10/7761http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-07-11T14:35:14.318312Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
title Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
spellingShingle Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
Sousa, Dyeime Ribeiro
Fasciolose
Fibrogênese
Imunoistoquímica
Ciências Agrárias
Bovino - Doenças
Fasciola hepática
title_short Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
title_full Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
title_fullStr Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
title_full_unstemmed Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
title_sort Caracterização do imunofenótipo das células envolvidas no processo fibrótico de fígados bovinos cronicamente infectados por Fasciola hepatica
author Sousa, Dyeime Ribeiro
author_facet Sousa, Dyeime Ribeiro
author_role author
dc.contributor.advisor1.fl_str_mv Nunes, Louisiane de Carvalho
dc.contributor.author.fl_str_mv Sousa, Dyeime Ribeiro
dc.contributor.referee1.fl_str_mv Hataka, Alessandre
dc.contributor.referee2.fl_str_mv Boeloni, J.N.
contributor_str_mv Nunes, Louisiane de Carvalho
Hataka, Alessandre
Boeloni, J.N.
dc.subject.por.fl_str_mv Fasciolose
Fibrogênese
Imunoistoquímica
topic Fasciolose
Fibrogênese
Imunoistoquímica
Ciências Agrárias
Bovino - Doenças
Fasciola hepática
dc.subject.cnpq.fl_str_mv Ciências Agrárias
dc.subject.br-rjbn.none.fl_str_mv Bovino - Doenças
Fasciola hepática
description The fascioliasis is a disease caused by the parasite Fasciola hepatica which can cause severe liver fibrosis. This study aimed to characterize the immunophenotype of cells involved in liver fibrogenesis of cattle with this disease. To this, 74 bovine livers condemned for fascioliasis were collected for histological processing and immunohistochemistry with anti-a-SMA, anti-elastin and anti-ER-TR7 antibodies. Was taken the classification of the degree of fibrosis followed by identification and quantification of each cell type in the parenchyma and portal space. Were used descriptive statistics and Spearman correlation test. In the right lobe of the liver 90.5% (67/74) was classified as fibrosis grade 1, 8.1% (6/74) as fibrosis grade 2 and 1.4% (01/74) as fibrosis grade 3. In the left hepatic lobe, 55.4% (41/74) of livers were classified as grade 1, 28.4% (21/74) as grade 2 and 16.2% (12/74) as grade 3. There were significant positive association (? = 0.41, p <0.0001) between the degree of fibrosis and hepatic lobe, revealing more fibrosis in the left lobe. Observed hepatic stellate cell (HSC) distributed in the parenchyma and myofibroblasts (MF) in portal spaces. No fibroblasts were found. The mean of HSC was 23.9 and there was significant correlation between the degree of fibrosis (? = -0.44, p <0.0001) and the amount of HSC (? = -0.61, p <0.0001) in the right and left lobes. The MF were found around the portal structures, venules, arterioles and biliar ducts and there was positive and significant correlation between the number of layers around each structure of the EP with the degree of fibrosis (? = 0.19, p <0.0001), though, has not been influenced by any structure of the portal space, in particular (p> 0.05). Concluded that the cells that participate in the fibrogenesis of the bovine livers with chronic fascioliasis are HSC and MF. These cells are involved in hepatic fibrogenesis because they increase in number with the progression of fibrosis
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-21
dc.date.accessioned.fl_str_mv 2018-08-01T22:56:53Z
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Mestrado em Ciências Veterinárias
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dc.publisher.department.fl_str_mv Centro de Ciências Agrárias e Engenharias
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Veterinárias
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