Efeitos de alguns tensoativos sobre a viabilidade celular de linhagens celulares de câncer de pulmão

• Autor(a) principal: Aguilar, Naidilene Chaves
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/5752
• topic: Lung carcinoma; Surfactants; Cell viability; Carcinoma de Pulmão; Fumo - vício; Tensoativos; Viabilidade celular; Pulmões - cancer; Biotecnologia; 61

The Lung Carcinoma (LG) represents the major challenge to the global health, becoming the leading cause of death by cancer among men and women in Brazil. The natural history of the disease includes high mortality rates and aggressive evolution, often with the patient coming to the doctor when the disease is already advanced. The incidence of this disease has its peak between the ages of 55 and 65 years old. The occurrence of lung cancer is intrinsically linked to the exposure to carcinogens, so that 90% of the cases are associated with active smoking. Thus, this study evaluated the feasibility of tumor lines through tests with four types of drugs of different classes. Recently, several experiments were performed with drugs that selectively inhibit the spread of tumor cells but have no effect on primary growth (Ross et al., 1969). This communication is in agreement with experiments performed to study the effect anti-metastatic of some drugs on the tumor spread. Thus, our study experiments to use the surfactants, Triton® X-100 (Sigma), Tween® 20 (BioAgency), SDS (Vetec) and CDs (Sigma) in varying concentrations, in order to evaluate cell viability. In this study we used two tumor cell lines, the H460 and A549, respectively classified as large cell carcinoma and adenocarcinoma. The screening of the substances with potential cytotoxic effect was done by the colorimetric test using MTT (3 - (4, 5-dimethyl-2-y1) 2, 5-diphenyl tetrazolium bromide), as described by Mosmann (1983). The results showed variations in cell viability shown by the strains under study when treated with different types of drugs resulting in a viability proportional to the type and concentration of the drug. He was taken into consideration the different classes and chemical structures of the substances tested to discuss the different results and different strains. The SDS showed greater cytotoxic effect and the CD with the lowest or no effect under the same 8 conditions, Tween 20 and Triton X-100 had similar effects, with Triton X-100 getting a greater reduction in cell viability.