O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2024 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
Texto Completo: | http://repositorio.ufes.br/handle/10/17410 |
Resumo: | Postmenopausal women have a higher incidence of acute myocardial infarction (MI) and a worse prognosis. The main cause of fibrosis and heart failure is MI. We previously demonstrated that Ellagic acid (EA) attenuates diastolic dysfunction by reducing cardiac oxidative stress. Here, we examined the effect of the administration of EA on cardiac fibrosis and other pathological effects induced by MI in female rats that have been exposed to estrogen deprivation. Ovariectomized Wistar rats were subjected to coronary artery ligation to induce MI. The EA (30 mg/kg) was administered by oral gavage daily for four weeks. Hemodynamic parameters were measured through cannulation of the carotid artery. Picrosirius red staining was used to evaluate collagen deposition and to determine the size of the infarction. MMP-8 expression was measured by immunofluorescence. Nitric oxide (NO) and reactive oxygen species (ROS) production were quantified by the DAF-2 and DHE probes, respectively. ELISA was used for the quantification of inflammatory cytokines. MI promoted ventricular dysfunction and cardiac fibrosis. Oral administration of EA reduced the size of the myocardial lesion, improved hemodynamic parameters, decreased the deposition of total collagen, and type I collagen, and increased the deposition of type III collagen. MMP-8 expression and IL-6 production were increased in the heart after MI and EA decreased them. Finally, the administration of EA increased the production of in situ NO and decreased the production of ROS. These data highlight that oral treatment with EA may be a potential natural therapeutic for menopausal women under cardiac stress. |
id |
UFES_5092e1eab8c7ee2314a586a253d648b8 |
---|---|
oai_identifier_str |
oai:repositorio.ufes.br:10/17410 |
network_acronym_str |
UFES |
network_name_str |
Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
repository_id_str |
2108 |
spelling |
76Simões, Simone Alves de Almeidahttps://orcid.org/0000-0002-9905-509Xhttp://lattes.cnpq.br/4036865618615755Abreu, Glaucia Rodrigues dehttps://orcid.org/0009-0008-8772-8470http://lattes.cnpq.br/0229590907405570Gonçalves, Laís Lopeshttps://orcid.org/0000-0002-8603-8354http://lattes.cnpq.br/0964569425731887Gouvea, Sônia Alveshttps://orcid.org/0000-0001-5180-471Xhttp://lattes.cnpq.br/7268228122543743Lemos, Virgínia Soareshttps://orcid.org/0000-0003-1234-9325http://lattes.cnpq.br/65755692643190712024-06-20T08:21:47Z2024-06-20T08:21:47Z2024-03-27Postmenopausal women have a higher incidence of acute myocardial infarction (MI) and a worse prognosis. The main cause of fibrosis and heart failure is MI. We previously demonstrated that Ellagic acid (EA) attenuates diastolic dysfunction by reducing cardiac oxidative stress. Here, we examined the effect of the administration of EA on cardiac fibrosis and other pathological effects induced by MI in female rats that have been exposed to estrogen deprivation. Ovariectomized Wistar rats were subjected to coronary artery ligation to induce MI. The EA (30 mg/kg) was administered by oral gavage daily for four weeks. Hemodynamic parameters were measured through cannulation of the carotid artery. Picrosirius red staining was used to evaluate collagen deposition and to determine the size of the infarction. MMP-8 expression was measured by immunofluorescence. Nitric oxide (NO) and reactive oxygen species (ROS) production were quantified by the DAF-2 and DHE probes, respectively. ELISA was used for the quantification of inflammatory cytokines. MI promoted ventricular dysfunction and cardiac fibrosis. Oral administration of EA reduced the size of the myocardial lesion, improved hemodynamic parameters, decreased the deposition of total collagen, and type I collagen, and increased the deposition of type III collagen. MMP-8 expression and IL-6 production were increased in the heart after MI and EA decreased them. Finally, the administration of EA increased the production of in situ NO and decreased the production of ROS. These data highlight that oral treatment with EA may be a potential natural therapeutic for menopausal women under cardiac stress.Mulheres na pós menopausa têm uma incidência mais alta de infarto agudo do miocárdio (IAM) e um pior prognóstico. A principal causa de fibrose e insuficiência cardíaca é o IAM. Demonstramos anteriormente que o ácido elágico (EA) atenua a disfunção diastólica reduzindo o estresse oxidativo cardíaco. Aqui, examinamos o efeito da administração de EA na fibrose cardíaca e outros efeitos patológicos induzidos pelo IAM em ratas fêmeas expostas à privação de estrogênio. Ratas Wistar ovariectomizadas foram submetidas à ligação da artéria coronária para induzir IAM. O EA (30 mg/kg) foi administrado por sonda oral diariamente durante quatro semanas. Parâmetros hemodinâmicos foram medidos através da cânulação da artéria carótida. A coloração de vermelho de Picrosirius foi usada para avaliar a deposição de colágeno e determinar o tamanho do infarto. A expressão de MMP-8 foi medida por imunofluorescência. A produção de óxido nítrico (NO) e espécies reativas de oxigênio (ROS) foi quantificada pelas sondas DAF-2 e DHE, respectivamente. ELISA foi usado para a quantificação de citocinas inflamatórias. O IAM promoveu disfunção ventricular e fibrose cardíaca. A administração oral de EA reduziu o tamanho da lesão miocárdica, melhorou os parâmetros hemodinâmicos, diminuiu a deposição de colágeno total e colágeno tipo I, e aumentou a deposição de colágeno tipo III. A expressão de MMP-8 e a produção de IL-6 aumentaram no coração após o IAM e o EA as diminuíram. Finalmente, a administração de EA aumentou a produção de NO in situ e diminuiu a produção de ROS. Estes dados destacam que o tratamento oral com EA pode ser uma terapia natural potencial para mulheres na menopausa sob estresse cardíaco.FAPESTexthttp://repositorio.ufes.br/handle/10/17410porUniversidade Federal do Espírito SantoMestrado em Ciências FisiológicasPrograma de Pós-Graduação em Ciências FisiológicasUFESBRCentro de Ciências da Saúdesubject.br-rjbnCiências da SaúdeInfartoovariectomiamenopausaácido elágicopolifenóisO ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadastitle.alternativeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESemail@ufes.brORIGINALLaisLopesGonçalves-2024-dissertacao.pdfLaisLopesGonçalves-2024-dissertacao.pdfapplication/pdf1556018http://repositorio.ufes.br/bitstreams/f151a85a-5f25-47af-b782-a9c286687f67/download9d7f93cf39c88d07c7cf963884aaf7a2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufes.br/bitstreams/bddb29c0-da66-4716-a55f-b0d637e8b586/download8a4605be74aa9ea9d79846c1fba20a33MD5210/174102024-08-29 11:25:07.002oai:repositorio.ufes.br:10/17410http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T18:00:48.193996Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)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 |
dc.title.none.fl_str_mv |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
dc.title.alternative.none.fl_str_mv |
title.alternative |
title |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
spellingShingle |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas Gonçalves, Laís Lopes Ciências da Saúde Infarto ovariectomia menopausa ácido elágico polifenóis subject.br-rjbn |
title_short |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
title_full |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
title_fullStr |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
title_full_unstemmed |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
title_sort |
O ácido elágico melhora o padrão funcional hemodinâmico e previne o remodelamento cardíaco após infarto agudo do miocárdio em ratas ovariectomizadas |
author |
Gonçalves, Laís Lopes |
author_facet |
Gonçalves, Laís Lopes |
author_role |
author |
dc.contributor.authorID.none.fl_str_mv |
https://orcid.org/0000-0002-8603-8354 |
dc.contributor.authorLattes.none.fl_str_mv |
http://lattes.cnpq.br/0964569425731887 |
dc.contributor.advisor-co1.fl_str_mv |
Simões, Simone Alves de Almeida |
dc.contributor.advisor-co1ID.fl_str_mv |
https://orcid.org/0000-0002-9905-509X |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4036865618615755 |
dc.contributor.advisor1.fl_str_mv |
Abreu, Glaucia Rodrigues de |
dc.contributor.advisor1ID.fl_str_mv |
https://orcid.org/0009-0008-8772-8470 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0229590907405570 |
dc.contributor.author.fl_str_mv |
Gonçalves, Laís Lopes |
dc.contributor.referee1.fl_str_mv |
Gouvea, Sônia Alves |
dc.contributor.referee1ID.fl_str_mv |
https://orcid.org/0000-0001-5180-471X |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7268228122543743 |
dc.contributor.referee2.fl_str_mv |
Lemos, Virgínia Soares |
dc.contributor.referee2ID.fl_str_mv |
https://orcid.org/0000-0003-1234-9325 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6575569264319071 |
contributor_str_mv |
Simões, Simone Alves de Almeida Abreu, Glaucia Rodrigues de Gouvea, Sônia Alves Lemos, Virgínia Soares |
dc.subject.cnpq.fl_str_mv |
Ciências da Saúde |
topic |
Ciências da Saúde Infarto ovariectomia menopausa ácido elágico polifenóis subject.br-rjbn |
dc.subject.por.fl_str_mv |
Infarto ovariectomia menopausa ácido elágico polifenóis |
dc.subject.br-rjbn.none.fl_str_mv |
subject.br-rjbn |
description |
Postmenopausal women have a higher incidence of acute myocardial infarction (MI) and a worse prognosis. The main cause of fibrosis and heart failure is MI. We previously demonstrated that Ellagic acid (EA) attenuates diastolic dysfunction by reducing cardiac oxidative stress. Here, we examined the effect of the administration of EA on cardiac fibrosis and other pathological effects induced by MI in female rats that have been exposed to estrogen deprivation. Ovariectomized Wistar rats were subjected to coronary artery ligation to induce MI. The EA (30 mg/kg) was administered by oral gavage daily for four weeks. Hemodynamic parameters were measured through cannulation of the carotid artery. Picrosirius red staining was used to evaluate collagen deposition and to determine the size of the infarction. MMP-8 expression was measured by immunofluorescence. Nitric oxide (NO) and reactive oxygen species (ROS) production were quantified by the DAF-2 and DHE probes, respectively. ELISA was used for the quantification of inflammatory cytokines. MI promoted ventricular dysfunction and cardiac fibrosis. Oral administration of EA reduced the size of the myocardial lesion, improved hemodynamic parameters, decreased the deposition of total collagen, and type I collagen, and increased the deposition of type III collagen. MMP-8 expression and IL-6 production were increased in the heart after MI and EA decreased them. Finally, the administration of EA increased the production of in situ NO and decreased the production of ROS. These data highlight that oral treatment with EA may be a potential natural therapeutic for menopausal women under cardiac stress. |
publishDate |
2024 |
dc.date.accessioned.fl_str_mv |
2024-06-20T08:21:47Z |
dc.date.available.fl_str_mv |
2024-06-20T08:21:47Z |
dc.date.issued.fl_str_mv |
2024-03-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufes.br/handle/10/17410 |
url |
http://repositorio.ufes.br/handle/10/17410 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
Text |
dc.publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo Mestrado em Ciências Fisiológicas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Fisiológicas |
dc.publisher.initials.fl_str_mv |
UFES |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo Mestrado em Ciências Fisiológicas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) instname:Universidade Federal do Espírito Santo (UFES) instacron:UFES |
instname_str |
Universidade Federal do Espírito Santo (UFES) |
instacron_str |
UFES |
institution |
UFES |
reponame_str |
Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
collection |
Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
bitstream.url.fl_str_mv |
http://repositorio.ufes.br/bitstreams/f151a85a-5f25-47af-b782-a9c286687f67/download http://repositorio.ufes.br/bitstreams/bddb29c0-da66-4716-a55f-b0d637e8b586/download |
bitstream.checksum.fl_str_mv |
9d7f93cf39c88d07c7cf963884aaf7a2 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES) |
repository.mail.fl_str_mv |
|
_version_ |
1813022566808289280 |