Avaliação de marcadores de remodelamento cardíaco (funcionais, bioquímicos e estruturais) em ratos submetidos a exercício resistido e tratamento com decanoato de nandrolona.

• Autor(a) principal: Lima, Ewelyne Miranda de
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/8077
• topic: Anabolic steroids; Angiotensin II; Cardiac hypertrophy; Cardiac remodeling; Cardiopulmonary reflex; Cytokines; Resistance training; Esteroides anabolizantes; Angiotensina II; Hipertrofia cardíaca; Remodelamento cardíaco; Reflexo cardiopulmonar; Citocinas; Treinamento resistido; Fisiologia; 612

Resistance training (RT) has often been associated with the abuse of anabolic androgenic steroids (AAS) by athletes and non-athletes. The aim of this study was to evaluate the effects of resistance training (RT) with or without nandrolone decanoate, with the levels of cytokines and activity of angiotensin-converting enzyme (ACE) in the heart, and the sensitivity of cardiopulmonary Bezold-Jarisch reflex (BJR). Male Wistar rats were divided into 3 groups: C (receiving vehicle, untrained); E (RT: after one week of adaptation water, rats were trained to jump into the water twice a week for 4 weeks), DN (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly) e DNE (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly, associated with RT). The BJR was analyzed by measuring bradycardic and hypotensive responses induced by administration in bolus of five serotonin randomized doses (2 to 32 g/kg body weight). Myocyte hypertrophy and collagen deposition in the matrix was determined by morphometric analysis of images from slides stained with H&E and picrosirius red respectively. The TNF-α levels in the heart and ACE activity was determined by Elisa assay. The RT promoted resting bradycardia in E group (bpm 312 ± 9, p <0.01) when compared with C group (360 ± 11 bpm) and ND did not influence this parameter NDE (bpm 324 ± 9, p <0.05), furthermore promoted physiological hypertrophy in myocytes without collagen deposition and no other changes were observed. The ND association with RT shown myocyte hypertrophy, type I collagen deposition, increase on TNF-α levels (C: 207 ± 9; E: 211 ± 8; DN: 269 ± 10; DNE: 242 ± 10 pg / mg, p <0,01) and ACE activity(C: 27,0 ± 7 ; E: 39,0 ± 6; DN: 50 ± 5; DNE: 65,0 ± 4 %, p <0,01), decreased IL-10 levels (C: 220 ± 7; E: 231 ± 5; DN: 165 ± 5; DNE: 164 ± 4 pg / mg, p<0,01), impairment in the sensitivity of BJR and hypertension(C: 104 ± 2 mmHg; E: 108 ± 8 mmHg; DN: 144 ± 7; DNE: 138 ± 5 mmHg; p <0,01). ND is associated with changes in cardiac structure and function as a result of the development of pathological cardiac hypertrophy (cytokine imbalance in heart elevation of ACE activity) and cardiac injury, and resistance training was unable to reverse the deleterious cardiovascular effects.