Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
Texto Completo: | http://repositorio.ufes.br/handle/10/8237 |
Resumo: | The Schinus terebinthifolia Raddi, popularly known as pepper tree, is in the National Medicinal Plants of the SUS’s Interest List and has great potential for growth inhibition of microorganisms, which makes this an alternative to medicinal plant antimicrobial activity. This study aims to evaluate the antimicrobial activity against pathogenic fungi (Colletotrichum gloeosporioides Penz and Colletotrichum musae) and against the bacteria which causes human infection (Staphylococcus aureus), from the extracts fractions obtained from leaves and stem bark of S. terebinthifolia, to identify secondary metabolic compounds in leaves and stem bark and quantify the compounds of the flavonoids group. The extractions of leaves and stem bark were prepared by maceration using increasing polarity solvents (hexane, dichloromethane and ethanol) and ethanol solvent for the preparation of crude extract that underwent liquid-liquid cut. The phytochemical screening of crude ethanol extracts of both parties and the quantification of flavonoids were performed. The fractions were tested for determining the minimum inhibitory concentration for bacteria and for mycelial growth inhibition of the fungus. The results showed that all tested microorganisms demonstrated sensitivity to the compounds found in the extracts of S. terebinthifolia regardless the type of extraction, and that the flavonoids are found in greater amounts in the leaves (52,93 µg.mL-1) than in the stem bark (9.58 µg.mL-1), possibly, together with tannins, they do the inhibitory activity of fungi and bacteria. Therefore, extracts of bark stem and leaves of the pepper tree may be a good way for controlling the growth of these pathogen and they can act as an herbal medicine with antibiotic effect as well as being a possible agent to control the diseases in papaya and banana plants. |
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Atividade antimicrobiana dos extratos de Schinus terebinthifolia RaddiAntimicrobial activity of extracts of Schinus terebinthifolia RaddiAnthracnoseAntibioticSchinus terebinthifoliaAntibióticosAroeira-vermelhaAntracnoseAgronomia63The Schinus terebinthifolia Raddi, popularly known as pepper tree, is in the National Medicinal Plants of the SUS’s Interest List and has great potential for growth inhibition of microorganisms, which makes this an alternative to medicinal plant antimicrobial activity. This study aims to evaluate the antimicrobial activity against pathogenic fungi (Colletotrichum gloeosporioides Penz and Colletotrichum musae) and against the bacteria which causes human infection (Staphylococcus aureus), from the extracts fractions obtained from leaves and stem bark of S. terebinthifolia, to identify secondary metabolic compounds in leaves and stem bark and quantify the compounds of the flavonoids group. The extractions of leaves and stem bark were prepared by maceration using increasing polarity solvents (hexane, dichloromethane and ethanol) and ethanol solvent for the preparation of crude extract that underwent liquid-liquid cut. The phytochemical screening of crude ethanol extracts of both parties and the quantification of flavonoids were performed. The fractions were tested for determining the minimum inhibitory concentration for bacteria and for mycelial growth inhibition of the fungus. The results showed that all tested microorganisms demonstrated sensitivity to the compounds found in the extracts of S. terebinthifolia regardless the type of extraction, and that the flavonoids are found in greater amounts in the leaves (52,93 µg.mL-1) than in the stem bark (9.58 µg.mL-1), possibly, together with tannins, they do the inhibitory activity of fungi and bacteria. Therefore, extracts of bark stem and leaves of the pepper tree may be a good way for controlling the growth of these pathogen and they can act as an herbal medicine with antibiotic effect as well as being a possible agent to control the diseases in papaya and banana plants.A Schinus terebinthifolia Raddi, conhecida popularmente como aroeira vermelha, se encontra na Relação Nacional de Plantas Medicinais de Interesse ao Sistema Único de Saúde e possui grande potencial inibitório de crescimento de microrganismo, o que torna esta planta medicinal uma alternativa para atividade antimicrobiana. Este estudo propõe avaliar a atividade antimicrobiana das frações de extratos obtidas a partir de folhas e de casca de caule de S. terebinthifolia, contra bactéria causadora de infecção humana (Staphylococcus aureus) e contra fungos fitopatogênicos (Colletotrichum gloeosporioides Penz e Colletotrichum musae), identificar os compostos do metabolismo secundário presentes nas folhas e nas cascas de caule e quantificar os flavonóides. As extrações das folhas e das cascas de caule foram preparadas por maceração, utilizando solventes de polaridades crescentes (hexano, diclorometano e etanol) e o solvente etanol para o preparo de extrato bruto, submetido ao fracionamento líquido-líquido. Foi realizada a triagem fitoquímica dos extratos brutos etanólicos de ambas as partes e a quantificação de flavonóides. As frações foram utilizadas nos testes de determinação da concentração inibitória mínima, para bactéria, e de inibição do crescimento micelial dos fungos. Os resultados obtidos demonstraram que todos os microrganismos testados apresentaram sensibilidade aos compostos encontrados nos extratos de S. terebinthifolia, independente do tipo de extração, e que nas folhas são encontrados flavonóides em maior quantidade (52,93 μg.mL-1) do que nas cascas de caule (9,58 μg.mL-1), que possivelmente juntamente com os taninos exercem a atividade inibitória dos fungos e bactérias. Portanto, extratos de casca de caule e de folhas de aroeira podem ser uma possibilidade para controlar o crescimento de tais patógenos, como um medicamento fitoterápico com efeito antimicrobiano, bem como um possível agente para controle da antracnose em cultura de mamão e de banana.Universidade Federal do Espírito SantoBRMestrado em Agricultura TropicalUFESPrograma de Pós-Graduação em Agricultura TropicalSilva, Marcelo Barreto daCarvalho, Camilo Amaro deRamos, Maria da Penha PiccoloRebello, Luciana Camizão2018-08-01T23:26:44Z2018-08-012018-08-01T23:26:44Z2013-11-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTextapplication/pdfREBELLO, Luciana Camizão. Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi. 2013. 48 f. Dissertação (Mestrado em Agricultura Tropical) - Programa de Pós-Graduação em Agricultura Tropical, Universidade Federal do Espírito Santo, São Mateus, 2013.http://repositorio.ufes.br/handle/10/8237porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFES2024-06-26T15:51:18Zoai:repositorio.ufes.br:10/8237Repositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-06-26T15:51:18Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false |
dc.title.none.fl_str_mv |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi Antimicrobial activity of extracts of Schinus terebinthifolia Raddi |
title |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
spellingShingle |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi Rebello, Luciana Camizão Anthracnose Antibiotic Schinus terebinthifolia Antibióticos Aroeira-vermelha Antracnose Agronomia 63 |
title_short |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
title_full |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
title_fullStr |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
title_full_unstemmed |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
title_sort |
Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi |
author |
Rebello, Luciana Camizão |
author_facet |
Rebello, Luciana Camizão |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Marcelo Barreto da Carvalho, Camilo Amaro de Ramos, Maria da Penha Piccolo |
dc.contributor.author.fl_str_mv |
Rebello, Luciana Camizão |
dc.subject.por.fl_str_mv |
Anthracnose Antibiotic Schinus terebinthifolia Antibióticos Aroeira-vermelha Antracnose Agronomia 63 |
topic |
Anthracnose Antibiotic Schinus terebinthifolia Antibióticos Aroeira-vermelha Antracnose Agronomia 63 |
description |
The Schinus terebinthifolia Raddi, popularly known as pepper tree, is in the National Medicinal Plants of the SUS’s Interest List and has great potential for growth inhibition of microorganisms, which makes this an alternative to medicinal plant antimicrobial activity. This study aims to evaluate the antimicrobial activity against pathogenic fungi (Colletotrichum gloeosporioides Penz and Colletotrichum musae) and against the bacteria which causes human infection (Staphylococcus aureus), from the extracts fractions obtained from leaves and stem bark of S. terebinthifolia, to identify secondary metabolic compounds in leaves and stem bark and quantify the compounds of the flavonoids group. The extractions of leaves and stem bark were prepared by maceration using increasing polarity solvents (hexane, dichloromethane and ethanol) and ethanol solvent for the preparation of crude extract that underwent liquid-liquid cut. The phytochemical screening of crude ethanol extracts of both parties and the quantification of flavonoids were performed. The fractions were tested for determining the minimum inhibitory concentration for bacteria and for mycelial growth inhibition of the fungus. The results showed that all tested microorganisms demonstrated sensitivity to the compounds found in the extracts of S. terebinthifolia regardless the type of extraction, and that the flavonoids are found in greater amounts in the leaves (52,93 µg.mL-1) than in the stem bark (9.58 µg.mL-1), possibly, together with tannins, they do the inhibitory activity of fungi and bacteria. Therefore, extracts of bark stem and leaves of the pepper tree may be a good way for controlling the growth of these pathogen and they can act as an herbal medicine with antibiotic effect as well as being a possible agent to control the diseases in papaya and banana plants. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-29 2018-08-01T23:26:44Z 2018-08-01 2018-08-01T23:26:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
REBELLO, Luciana Camizão. Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi. 2013. 48 f. Dissertação (Mestrado em Agricultura Tropical) - Programa de Pós-Graduação em Agricultura Tropical, Universidade Federal do Espírito Santo, São Mateus, 2013. http://repositorio.ufes.br/handle/10/8237 |
identifier_str_mv |
REBELLO, Luciana Camizão. Atividade antimicrobiana dos extratos de Schinus terebinthifolia Raddi. 2013. 48 f. Dissertação (Mestrado em Agricultura Tropical) - Programa de Pós-Graduação em Agricultura Tropical, Universidade Federal do Espírito Santo, São Mateus, 2013. |
url |
http://repositorio.ufes.br/handle/10/8237 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
Text application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo BR Mestrado em Agricultura Tropical UFES Programa de Pós-Graduação em Agricultura Tropical |
publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo BR Mestrado em Agricultura Tropical UFES Programa de Pós-Graduação em Agricultura Tropical |
dc.source.none.fl_str_mv |
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Universidade Federal do Espírito Santo (UFES) |
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Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
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Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES) |
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