Análise da expressão de Patched e distribuição de mastócitos em tumores odontogênicos ceratocísticos

• Autor(a) principal: Milholli, Ana Flávia Lagassa
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/8188
• topic: Keratocystic odontogenic tumor; Mast cells; Tumor odontogênico ceratocístico; Patched; Mastócitos; Odontologia; Tumores odontogênicos; 616.314

The Keratocystic Odontogenic Tumor (KCOT) is an intraosseous benign tumor, which displays aggressive behavior, with a tendency to local destruction and infiltrative and recurrent potential. It is also one of the manifestations of the Nevoid Basal Cell Carcinoma Syndrome (NBCCS). Evidence shows that the patched gene may be a significant factor in the development of KCOTs by having their activity inactivated. The aim of this study was to perform a comparative analysis between sporadic and syndromic KCOTs by checking the expression pattern of Patched protein and the distribution pattern of mast cells. Twenty-eight specimens of KCOTs recorded in Anatomic Pathology Service (SAP) of the School of Dentistry of the Federal University of Espirito Santo (UFES) during the period 2004-2012 were submitted to immunohistochemical technique to detect Patched protein and stained with Alcian Blue to detect mast cells. Histopathologic features were evaluated. All clinical data (gender, age, ethnicity and location of the lesion) were obtained from the files. For immunohistochemical analysis a score was adopted. Regarding the quantitative expression of patched, we found that 77.77% of KCOTs (syndromic and non-syndromic) showed immunostaining score of 4 (between 76% and 100% of cells stained). The basal and intermediate epithelial layers comprised most of staining compared to the surperficial layer. Only cytoplasmic staining was found. The histopathologic features were more evident in syndromic tumors, however, there was no significant difference in mean mast cells found to syndromic and non-syndromic tumors. Therefore, our findings demonstrated a strong epithelial expression of Patched in KCOTs, suggesting that this protein seems to be important for the pathogenesis of these tumors.