Efeito da longevidade na resposta a alta pressão hidrostática de Saccharomyces cerevisiae

• Autor(a) principal: Spagnol, Brigida de Almeida Amorim
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/7128
• topic: High hydrostatic pressure (HHP); Stress; Longevity; Aging; Alta pressão hidrostática (HHP); Saccharomyces cerevisiae; Estresse; Longevidade; Envelhecimento; Biotecnologia; 61

A strong relationship between the activation of regulatory pathways in response to stress, longevity and aging has been pointed out in many studies. The causes of aging are multifactorial, affecting a series of cell mechanisms, but these regulatory pathways among eukaryotes are surprisingly well-conserved. The maintenance of an efficient stress response mechanism can be a potential strategy to extend longevity and to delay aging and the appearance of age-associated diseases. In research addressing longevity and aging, model organisms such as the yeast Saccharomyces cerevisiae are being used, since the pathways involved in energy balance, damage accumulation, stress response, genomic integrity, apoptosis, and other mechanisms are also preserved in these cells. Thus, the purpose of this study was to evaluate the effects of high hydrostatic pressure (HHP) on mother and daughter cells of S. cerevisiae, to investigate whether longevity favors stress response. To achieve these goals mother cells and daughter cell were separated through of affinity chromatography method, where mother cells had their cell surface marked with biotin and streptavidin containing magnetics microbeads. After that, it was allowed that the mother cells could generate unlabeled daughter cells. Thus, the inoculum was added to a magnetic column where the labeled cells were retained and the unlabeled eluted into a test-tube. To cause stress was used the HHP that is a useful tool to induce oxidative stress in S. cerevisiae, making it possible to analyze yeast survival and gene expression involved in stress response, longevity and aging. We observed huge differences of the mother and daughter cells, between survival rates as well as between the results of gene expression. So that the first group of cells produced better results in the adaptative response to stress than the second group. It was therefore concluded that stress contributes to longevity, and longevity in turn proved important for the most effective adaptive response mechanisms to stress stimuli.