Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal

Detalhes bibliográficos
Autor(a) principal: Feletti, Mariana Pires
Data de Publicação: 2024
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/17453
Resumo: The present study aimed to evaluate the prevalence of Candida spp. in lesions that affect the oral mucosa, assess the distribution of Candida species present, and analyze the risk factors associated with this relationship. A cross-sectional study was conducted in which patients with lesions on the oral mucosa underwent swab collection to investigate Candida spp. The first collection was carried out in the lesion area, and a second in the mucosa region without apparent lesion. Patient samples were plated on CHROMagar Candida™, and colony-forming unit counts were performed to determine fungal load. Biochemical, morphological, and mass spectrometry tests were carried out to identify the species, followed by in vitro sensitivity tests to antifungals. The data collected indicated a prevalence of 40% of Candida spp. associated with oral lesions in x patients evaluated. Among the most commonly found species, C. albicans (62.5%), C. glabrata (10.5%), C. parapsilosis (7.5%), and C. tropicalis (7.5%) stood out. It was observed that the presence of the lesion was a factor that influenced the occurrence of Candida spp., with a more significant occurrence of this microorganism in areas of altered mucosa. Furthermore, Candida spp. it was more prevalent in elderly, female, and diabetic patients. No association was identified between Candida spp. and lesions in the oral mucosa with hyposalivation, history of smoking, and alcohol consumption. Of the 37 samples submitted to antifungal susceptibility testing, all have a resistance profile to Nystatin. Based on the data from this study, it is concluded that lesions in the oral mucosa represent a risk factor for colonization by Candida spp. Although most species identified are C. albicans, nonalbicans species were isolated in the oral cavity of patients, emphasizing C. parapsilosis. Understanding the relationship between species and lesions in the oral mucosa is crucial for safer and more effective patient clinical management.
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spelling 80Tavares, Sarah Gonçalveshttps://orcid.org/0000-0003-4170-4445http://lattes.cnpq.br/1018708169217296Velloso, Tânia Regina Grãohttps://orcid.org/0000-0001-6865-7955http://lattes.cnpq.br/6427157237870625Feletti, Mariana Pireshttps://orcid.org/0000-0003-2619-5953http://lattes.cnpq.br/0978973634199704Melo, Analy Salles de Azevedohttps://orcid.org/0000-0001-6900-6468http://lattes.cnpq.br/8922840487452492Barroso, Danielle Resende Camisascahttps://orcid.org/0000-0003-1193-2113http://lattes.cnpq.br/39890745931816742024-06-21T12:53:19Z2024-06-21T12:53:19Z2024-03-12The present study aimed to evaluate the prevalence of Candida spp. in lesions that affect the oral mucosa, assess the distribution of Candida species present, and analyze the risk factors associated with this relationship. A cross-sectional study was conducted in which patients with lesions on the oral mucosa underwent swab collection to investigate Candida spp. The first collection was carried out in the lesion area, and a second in the mucosa region without apparent lesion. Patient samples were plated on CHROMagar Candida™, and colony-forming unit counts were performed to determine fungal load. Biochemical, morphological, and mass spectrometry tests were carried out to identify the species, followed by in vitro sensitivity tests to antifungals. The data collected indicated a prevalence of 40% of Candida spp. associated with oral lesions in x patients evaluated. Among the most commonly found species, C. albicans (62.5%), C. glabrata (10.5%), C. parapsilosis (7.5%), and C. tropicalis (7.5%) stood out. It was observed that the presence of the lesion was a factor that influenced the occurrence of Candida spp., with a more significant occurrence of this microorganism in areas of altered mucosa. Furthermore, Candida spp. it was more prevalent in elderly, female, and diabetic patients. No association was identified between Candida spp. and lesions in the oral mucosa with hyposalivation, history of smoking, and alcohol consumption. Of the 37 samples submitted to antifungal susceptibility testing, all have a resistance profile to Nystatin. Based on the data from this study, it is concluded that lesions in the oral mucosa represent a risk factor for colonization by Candida spp. Although most species identified are C. albicans, nonalbicans species were isolated in the oral cavity of patients, emphasizing C. parapsilosis. Understanding the relationship between species and lesions in the oral mucosa is crucial for safer and more effective patient clinical management.O presente estudo teve como objetivo avaliar a prevalência de Candida spp. em lesões que afetam a mucosa bucal, avaliar a distribuição de espécies de Candida presentes e analisar os fatores associados. Foi conduzido um estudo transversal seccional, no qual pacientes com lesões na mucosa bucal foram submetidos à coleta com swab para investigação de Candida spp. A primeira coleta foi realizada na área da lesão, e uma segunda na região da mucosa sem lesão aparente. As amostras dos pacientes foram semeadas em CHROMagar Candida™ , e a contagem das unidades formadoras de colônias foi realizada para determinar a carga fúngica. Testes bioquímicos, morfológico e espectrometria de massas foram conduzidos para a identificação das espécies, seguidos por testes de sensibilidade in vitro aos antifúngicos (TSA). Os dados coletados indicaram uma prevalência de 40% de Candida spp. associadas às lesões bucais em oitenta pacientes avaliados. Entre as espécies mais comumente encontradas, destacaram-se C. albicans (62,5%), C. glabrata (10,5%), C. Parapsilosis (7,5%), e C. tropicalis (7,5%). Observou-se que a presença da lesão foi um fator que influenciou na ocorrência de Candida spp., com uma maior ocorrência deste microrganismo em áreas da mucosa alterada. Além disso, Candida spp. foi mais prevalente em pacientes idosos, do sexo feminino e diabéticos. Não foi identificada uma associação entre Candida spp. e lesões na mucosa bucal com hipossalivação, histórico de tabagismo e etilismo. Das 37 amostras submetidas ao teste de sensibilidade a antifúngicos, todas caracterizam um perfil de resistência CIMs elevados para nistatina. Com base nos dados deste estudo, conclui-se que as lesões na mucosa bucal representam um fator de risco para a colonização por Candida spp. Embora a maioria das espécies identificadas seja de C. albicans, espécies não-albicans foram isoladas na cavidade bucal dos pacientes, com destaque para C. parapsilosis. A compreensão da relação entre as espécies e as lesões na mucosa bucal é crucial para um manejo clínico mais seguro e eficaz do paciente.CAPESTexthttp://repositorio.ufes.br/handle/10/17453porUniversidade Federal do Espírito SantoMestrado em Ciências OdontológicasPrograma de Pós-Graduação em Ciências OdontológicasUFESBRCentro de Ciências da Saúdesubject.br-rjbnÁrea(s) do conhecimento do documento (Tabela CNPq)CandidaCandidíase oralEstomatologiaantifúngicosPrevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucaltitle.alternativeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESemail@ufes.brORIGINALMarianaPiresFeletti-2024-dissertacao.pdfMarianaPiresFeletti-2024-dissertacao.pdfapplication/pdf5997211http://repositorio.ufes.br/bitstreams/2c62951f-756b-4e7f-894c-db5bf2ebe06e/downloadb5b3bd49b2a2c955b5527b33c93e140aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufes.br/bitstreams/77a1f839-7259-4bb7-afd8-ee535ef61087/download8a4605be74aa9ea9d79846c1fba20a33MD5210/174532024-08-29 11:25:10.621oai:repositorio.ufes.br:10/17453http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T17:59:13.825205Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)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
dc.title.none.fl_str_mv Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
dc.title.alternative.none.fl_str_mv title.alternative
title Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
spellingShingle Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
Feletti, Mariana Pires
Área(s) do conhecimento do documento (Tabela CNPq)
Candida
Candidíase oral
Estomatologia
antifúngicos
subject.br-rjbn
title_short Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
title_full Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
title_fullStr Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
title_full_unstemmed Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
title_sort Prevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal
author Feletti, Mariana Pires
author_facet Feletti, Mariana Pires
author_role author
dc.contributor.authorID.none.fl_str_mv https://orcid.org/0000-0003-2619-5953
dc.contributor.authorLattes.none.fl_str_mv http://lattes.cnpq.br/0978973634199704
dc.contributor.advisor-co1.fl_str_mv Tavares, Sarah Gonçalves
dc.contributor.advisor-co1ID.fl_str_mv https://orcid.org/0000-0003-4170-4445
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1018708169217296
dc.contributor.advisor1.fl_str_mv Velloso, Tânia Regina Grão
dc.contributor.advisor1ID.fl_str_mv https://orcid.org/0000-0001-6865-7955
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6427157237870625
dc.contributor.author.fl_str_mv Feletti, Mariana Pires
dc.contributor.referee1.fl_str_mv Melo, Analy Salles de Azevedo
dc.contributor.referee1ID.fl_str_mv https://orcid.org/0000-0001-6900-6468
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/8922840487452492
dc.contributor.referee2.fl_str_mv Barroso, Danielle Resende Camisasca
dc.contributor.referee2ID.fl_str_mv https://orcid.org/0000-0003-1193-2113
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/3989074593181674
contributor_str_mv Tavares, Sarah Gonçalves
Velloso, Tânia Regina Grão
Melo, Analy Salles de Azevedo
Barroso, Danielle Resende Camisasca
dc.subject.cnpq.fl_str_mv Área(s) do conhecimento do documento (Tabela CNPq)
topic Área(s) do conhecimento do documento (Tabela CNPq)
Candida
Candidíase oral
Estomatologia
antifúngicos
subject.br-rjbn
dc.subject.por.fl_str_mv Candida
Candidíase oral
Estomatologia
antifúngicos
dc.subject.br-rjbn.none.fl_str_mv subject.br-rjbn
description The present study aimed to evaluate the prevalence of Candida spp. in lesions that affect the oral mucosa, assess the distribution of Candida species present, and analyze the risk factors associated with this relationship. A cross-sectional study was conducted in which patients with lesions on the oral mucosa underwent swab collection to investigate Candida spp. The first collection was carried out in the lesion area, and a second in the mucosa region without apparent lesion. Patient samples were plated on CHROMagar Candida™, and colony-forming unit counts were performed to determine fungal load. Biochemical, morphological, and mass spectrometry tests were carried out to identify the species, followed by in vitro sensitivity tests to antifungals. The data collected indicated a prevalence of 40% of Candida spp. associated with oral lesions in x patients evaluated. Among the most commonly found species, C. albicans (62.5%), C. glabrata (10.5%), C. parapsilosis (7.5%), and C. tropicalis (7.5%) stood out. It was observed that the presence of the lesion was a factor that influenced the occurrence of Candida spp., with a more significant occurrence of this microorganism in areas of altered mucosa. Furthermore, Candida spp. it was more prevalent in elderly, female, and diabetic patients. No association was identified between Candida spp. and lesions in the oral mucosa with hyposalivation, history of smoking, and alcohol consumption. Of the 37 samples submitted to antifungal susceptibility testing, all have a resistance profile to Nystatin. Based on the data from this study, it is concluded that lesions in the oral mucosa represent a risk factor for colonization by Candida spp. Although most species identified are C. albicans, nonalbicans species were isolated in the oral cavity of patients, emphasizing C. parapsilosis. Understanding the relationship between species and lesions in the oral mucosa is crucial for safer and more effective patient clinical management.
publishDate 2024
dc.date.accessioned.fl_str_mv 2024-06-21T12:53:19Z
dc.date.available.fl_str_mv 2024-06-21T12:53:19Z
dc.date.issued.fl_str_mv 2024-03-12
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dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Odontológicas
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dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Odontológicas
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