Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fernanda Mirella Garcia de
Data de Publicação: 2023
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/12439
Resumo: The infusion of Eugenia uniflora L. leaves is popularly used to treat viral and gastric symptoms, and the effectiveness of these treatments can be associated with the presence of flavonoids and tannins in its leaves. Eugenia uniflora L. is one of the species included in the National List of Medicinal Plants of Interest to the Unified Health System (RENISUS) and listed in the DRC on March 10, 2010 due to its importance in popular medicine. Furthermore, its leaves are used to treat sore throats, diarrhea, infections and gastrointestinal disorders. Among the secondary metabolites present in its composition, and which already have biological activities reported in the literature, flavonoids and tannins can be mentioned. Myricitrin is one of the flavonoids found abundantly in the leaves of Eugenia uniflora L. and has been associated with antioxidant, anti-inflammatory, antidiabetic, antihypertensive and antiviral activity. Oenotein B and gemin D/hipommannin A also have several related activities, including antibacterial activity. Thus, the objective of this study was to verify the anti-Helicobacter pylori activity and cytotoxicity of the extract and fractions of Eugenia uniflora L. leaves on gastric adenocarcinoma cells (AGS). Furthermore, verify better leaf collection conditions considering different locations (between mangrove, beach and mountain regions) and times (8:00 am, 1:00 pm and 6:00 pm), in addition to proposing FTIRPCA as a low-cost screening method for obtaining bioactive samples. The extracts and fractions had their chemical profiles determined by ESI(-)FT-ICR MS, ESI(-)FT-ICR MS/MS, ESI(-)LTQ MS and MSn. The aqueous fraction, rich in tannins, was considered the most active sample for Helicobacter pylori (MBC of 1024 µg/mL and MIC of 256 µg/mL) and the most cytotoxic for AGS cells (IC50 of 178 µg/mL). The strain analyzed showed resistance to metronidazole (MBC >1024 µg/mL and MIC of 512 µg/mL). For the FTIR-PCA analysis of the collection location, the samples from the beach and mangrove region were located in the PC1 > 0 direction, but separated along PC2. Those from the mountainous region were located at PC1 < 0. Regarding the separation by collection time, the samples collected at 1:00 pm were grouped at PC1 > 0. Considering the TIC values of the mass spectra and FTIR-PCA, the sample with the highest abundance of flavonoids is the one collected in the beach region at 1:00 p.m. In conclusion, the greatest cytotoxicity on AGS cells and anti Helicobacter pylori activity was found for the aqueous fraction of Eugenia uniflora L. leaves, which may be directly related to the presence of the tannins oenotein B and gemin D/hippomannin A. Finally, The best collection location and time to obtain bioactive extracts is at 1:00 pm in the beach area due to the high incidence of UV rays.
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spelling Kuster, Ricardo Machadohttps://orcid.org/0000000289615348http://lattes.cnpq.br/4149814906786366Oliveira, Fernanda Mirella Garcia dehttps://orcid.org/0000-0002-0627-223Xhttp://lattes.cnpq.br/7757710237470773Filgueiras, Paulo Robertohttps://orcid.org/0000000326171601http://lattes.cnpq.br/1907915547207861Gomes, Anne Caroline CandidoFranca, Hildegardo Seiberthttp://lattes.cnpq.br/1284874997224988Borges, Warley de Souzahttps://orcid.org/0000000344751028http://lattes.cnpq.br/97424022859704292024-05-29T20:55:16Z2024-05-29T20:55:16Z2023-11-17The infusion of Eugenia uniflora L. leaves is popularly used to treat viral and gastric symptoms, and the effectiveness of these treatments can be associated with the presence of flavonoids and tannins in its leaves. Eugenia uniflora L. is one of the species included in the National List of Medicinal Plants of Interest to the Unified Health System (RENISUS) and listed in the DRC on March 10, 2010 due to its importance in popular medicine. Furthermore, its leaves are used to treat sore throats, diarrhea, infections and gastrointestinal disorders. Among the secondary metabolites present in its composition, and which already have biological activities reported in the literature, flavonoids and tannins can be mentioned. Myricitrin is one of the flavonoids found abundantly in the leaves of Eugenia uniflora L. and has been associated with antioxidant, anti-inflammatory, antidiabetic, antihypertensive and antiviral activity. Oenotein B and gemin D/hipommannin A also have several related activities, including antibacterial activity. Thus, the objective of this study was to verify the anti-Helicobacter pylori activity and cytotoxicity of the extract and fractions of Eugenia uniflora L. leaves on gastric adenocarcinoma cells (AGS). Furthermore, verify better leaf collection conditions considering different locations (between mangrove, beach and mountain regions) and times (8:00 am, 1:00 pm and 6:00 pm), in addition to proposing FTIRPCA as a low-cost screening method for obtaining bioactive samples. The extracts and fractions had their chemical profiles determined by ESI(-)FT-ICR MS, ESI(-)FT-ICR MS/MS, ESI(-)LTQ MS and MSn. The aqueous fraction, rich in tannins, was considered the most active sample for Helicobacter pylori (MBC of 1024 µg/mL and MIC of 256 µg/mL) and the most cytotoxic for AGS cells (IC50 of 178 µg/mL). The strain analyzed showed resistance to metronidazole (MBC >1024 µg/mL and MIC of 512 µg/mL). For the FTIR-PCA analysis of the collection location, the samples from the beach and mangrove region were located in the PC1 > 0 direction, but separated along PC2. Those from the mountainous region were located at PC1 < 0. Regarding the separation by collection time, the samples collected at 1:00 pm were grouped at PC1 > 0. Considering the TIC values of the mass spectra and FTIR-PCA, the sample with the highest abundance of flavonoids is the one collected in the beach region at 1:00 p.m. In conclusion, the greatest cytotoxicity on AGS cells and anti Helicobacter pylori activity was found for the aqueous fraction of Eugenia uniflora L. leaves, which may be directly related to the presence of the tannins oenotein B and gemin D/hippomannin A. Finally, The best collection location and time to obtain bioactive extracts is at 1:00 pm in the beach area due to the high incidence of UV rays.A infusão das folhas de Eugenia uniflora L. é utilizada popularmente no tratamento de sintomas virais e gástricos, e, a efetividade desses tratamentos pode ser associada à presença de flavonoides e taninos em suas folhas. A Eugenia uniflora L. é uma das espécies incluídas na Relação Nacional de Plantas Medicinais de Interesse ao Sistema Único de Saúde (RENISUS) e listada na RDC no10 de março de 2010 devido à sua importância na medicina popular. Além disso, suas folhas são utilizadas no tratamento de dores de garganta, diarreia, infecções e distúrbios gastrointestinais. Dentre os metabólitos secundários presentes em sua composição, e que já possuem atividades biológicas relatadas em literatura, podem ser citados os flavonoides e taninos. A miricitrina é um dos flavonoides encontrados de maneira abundante em folhas de Eugenia uniflora L. e que tem sido associada à atividade antioxidante, antiinflamatória, antidiabética, anti-hipertensiva e antiviral. A oenoteína B e a gemina D/ hipommanina A também possuem diversas atividades relatadas, entre estas a atividade antibacteriana. Dessa forma, O objetivo deste estudo foi verificar a atividade anti- H.pylori e a citotoxicidade do extrato e frações de folhas de Eugenia uniflora L. em células de adenocarcinoma gástrico (AGS). Além disso, verificar melhores condições de coleta das folhas considerando diferentes localidades (entre as regiões de mangue, praia e montanha) e horários (8h00min, 13h00min e 18h00min), além de propor o FTIR-PCA como método de triagem de baixo custo para a obtenção de amostras bioativas. Os extratos e frações tiveram seus perfis químicos determinados por ESI(-)FT-ICR MS, ESI(-)FT-ICR MS/MS, ESI(-)LTQ MS e MSn . A fração aquosa, rica em taninos, foi considerada a amostra mais ativa para H. pylori (CBM de 1024 µg/mL e CMI de 256 µg/mL) e a mais citotoxica para células AGS (CI50 de 178 µg/mL). A cepa analisada apresentou resistência ao metronidazol (CBM >1024 µg/mL e CMI de 512 µg/mL). Para a análise de FTIR-PCA da localidade de coleta, as amostras da região de praia e mangue localizaram-se na direção PC1 > 0, porém separadas ao longo de PC2. As da região serrana localizaram-se em PC1 < 0. Em relação à separação por horário de coleta, as amostras coletadas às 13h00min agruparam-se em PC1 > 0. Considerando os valores de TIC dos espectros de massas e FTIR-PCA, a amostra com maior abundancia de flavonoides é a coletada na região de praia as 13h00min. Em conclusão, a maior citotoxicidade sobre células AGS e atividade anti H. pylori foi encontrada para a fração aquosa das folhas de E. uniflora L. podendo ser estas diretamente relacionadas a presença dos taninos oenoteina B e gemina D/hippomanina A. Por fim, o melhor local e horario de coleta para obtenção de extratos bioativos é as 13h00min na região de praia devida a alta incidência de raios UV.Texthttp://repositorio.ufes.br/handle/10/12439porUniversidade Federal do Espírito SantoDoutorado em QuímicaPrograma de Pós-Graduação em QuímicaUFESBRCentro de Ciências ExatasQuímicaE. uniflora L.Compostos fenólicosCitotoxicidadeH. pyloriEspectrometria de massasQuimiometriaCompostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pyloriinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALFernandaMirellaGarciadeOliveira-2023-tese.pdfapplication/pdf4182044http://repositorio.ufes.br/bitstreams/a34af609-b17c-44b7-a146-cf1458daed7f/download8e9264d0e7239c5e6ec06808a2466d00MD5110/124392024-09-11 13:19:56.445oai:repositorio.ufes.br:10/12439http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T17:52:45.581101Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
title Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
spellingShingle Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
Oliveira, Fernanda Mirella Garcia de
Química
E. uniflora L.
Compostos fenólicos
Citotoxicidade
H. pylori
Espectrometria de massas
Quimiometria
title_short Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
title_full Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
title_fullStr Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
title_full_unstemmed Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
title_sort Compostos fenólicos em folhas de Eugenia uniflora L.: atividade citotóxica em adenocarcinoma gástrico (células AGS) e atividade anti-Helicobacter pylori
author Oliveira, Fernanda Mirella Garcia de
author_facet Oliveira, Fernanda Mirella Garcia de
author_role author
dc.contributor.authorID.none.fl_str_mv https://orcid.org/0000-0002-0627-223X
dc.contributor.authorLattes.none.fl_str_mv http://lattes.cnpq.br/7757710237470773
dc.contributor.advisor1.fl_str_mv Kuster, Ricardo Machado
dc.contributor.advisor1ID.fl_str_mv https://orcid.org/0000000289615348
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4149814906786366
dc.contributor.author.fl_str_mv Oliveira, Fernanda Mirella Garcia de
dc.contributor.referee1.fl_str_mv Filgueiras, Paulo Roberto
dc.contributor.referee1ID.fl_str_mv https://orcid.org/0000000326171601
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1907915547207861
dc.contributor.referee2.fl_str_mv Gomes, Anne Caroline Candido
dc.contributor.referee3.fl_str_mv Franca, Hildegardo Seibert
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1284874997224988
dc.contributor.referee4.fl_str_mv Borges, Warley de Souza
dc.contributor.referee4ID.fl_str_mv https://orcid.org/0000000344751028
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/9742402285970429
contributor_str_mv Kuster, Ricardo Machado
Filgueiras, Paulo Roberto
Gomes, Anne Caroline Candido
Franca, Hildegardo Seibert
Borges, Warley de Souza
dc.subject.cnpq.fl_str_mv Química
topic Química
E. uniflora L.
Compostos fenólicos
Citotoxicidade
H. pylori
Espectrometria de massas
Quimiometria
dc.subject.por.fl_str_mv E. uniflora L.
Compostos fenólicos
Citotoxicidade
H. pylori
Espectrometria de massas
Quimiometria
description The infusion of Eugenia uniflora L. leaves is popularly used to treat viral and gastric symptoms, and the effectiveness of these treatments can be associated with the presence of flavonoids and tannins in its leaves. Eugenia uniflora L. is one of the species included in the National List of Medicinal Plants of Interest to the Unified Health System (RENISUS) and listed in the DRC on March 10, 2010 due to its importance in popular medicine. Furthermore, its leaves are used to treat sore throats, diarrhea, infections and gastrointestinal disorders. Among the secondary metabolites present in its composition, and which already have biological activities reported in the literature, flavonoids and tannins can be mentioned. Myricitrin is one of the flavonoids found abundantly in the leaves of Eugenia uniflora L. and has been associated with antioxidant, anti-inflammatory, antidiabetic, antihypertensive and antiviral activity. Oenotein B and gemin D/hipommannin A also have several related activities, including antibacterial activity. Thus, the objective of this study was to verify the anti-Helicobacter pylori activity and cytotoxicity of the extract and fractions of Eugenia uniflora L. leaves on gastric adenocarcinoma cells (AGS). Furthermore, verify better leaf collection conditions considering different locations (between mangrove, beach and mountain regions) and times (8:00 am, 1:00 pm and 6:00 pm), in addition to proposing FTIRPCA as a low-cost screening method for obtaining bioactive samples. The extracts and fractions had their chemical profiles determined by ESI(-)FT-ICR MS, ESI(-)FT-ICR MS/MS, ESI(-)LTQ MS and MSn. The aqueous fraction, rich in tannins, was considered the most active sample for Helicobacter pylori (MBC of 1024 µg/mL and MIC of 256 µg/mL) and the most cytotoxic for AGS cells (IC50 of 178 µg/mL). The strain analyzed showed resistance to metronidazole (MBC >1024 µg/mL and MIC of 512 µg/mL). For the FTIR-PCA analysis of the collection location, the samples from the beach and mangrove region were located in the PC1 > 0 direction, but separated along PC2. Those from the mountainous region were located at PC1 < 0. Regarding the separation by collection time, the samples collected at 1:00 pm were grouped at PC1 > 0. Considering the TIC values of the mass spectra and FTIR-PCA, the sample with the highest abundance of flavonoids is the one collected in the beach region at 1:00 p.m. In conclusion, the greatest cytotoxicity on AGS cells and anti Helicobacter pylori activity was found for the aqueous fraction of Eugenia uniflora L. leaves, which may be directly related to the presence of the tannins oenotein B and gemin D/hippomannin A. Finally, The best collection location and time to obtain bioactive extracts is at 1:00 pm in the beach area due to the high incidence of UV rays.
publishDate 2023
dc.date.issued.fl_str_mv 2023-11-17
dc.date.accessioned.fl_str_mv 2024-05-29T20:55:16Z
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dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Doutorado em Química
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências Exatas
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Doutorado em Química
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