Resposta a estresses consecutivos em Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Costa, Ane Catarine Tosi
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/7103
Resumo: The yeast Saccharomyces cerevisiae yeast plays an important role in industry due to its high fermentative capacity. During fermentation, there are constant changes in the conditions of the medium, exposing the yeasts to a series of simultaneous or sequential stresses and an efficient adaptation can lead to increased productivity and a improvement of their fermentative performance. In S. cerevisiae, adaptation involves an organized mobilization of genes called environmental stress response (ESR). Hsp12 is a protein belonging to families of heat shock proteins (HSPs) and this, in addition to maintaining the internal organization of the cell and increasing the flexibility of the cell wall and plasma membrane, is used as a stress reporter gene because its induction is in Largely through ESR already being used as a marker of stress status in yeast. Thus, the present work outlined a protocol of consecutive stress studies in order to evaluate morphological modifications and production of Hsp12 protein in S.cerevisiae. The results showed a similar variation of size of the mother cells and daughters in successive stresses compared to the growth of these cells in medium without stress addition. Cell cycle arrest was also a characteristic observed in both cells in consecutive stresses. The production of Hsp12 was higher in response to osmotic stress compared to oxidative and alcoholic stress in the isolated treatments, but the concentration of this protein in the latter two increased when the cell was exposed to consecutive stresses. This increase may be justified by the cross-protection of the acquired cell after contact with a high osmolarity solution. The difference in the results of the response to isolated and successive stresses shows that this methodology is more efficient to understand the behavior of the cell, because it is similar to the environment in the fermentation tanks.
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spelling Fernandes, Antônio Alberto RibeiroFernandes, Patrícia Machado BuenoCosta, Ane Catarine TosiVentura, José AiresSantos, Alexandre Martins Costa2018-08-01T21:35:00Z2018-08-012018-08-01T21:35:00Z2017-03-09The yeast Saccharomyces cerevisiae yeast plays an important role in industry due to its high fermentative capacity. During fermentation, there are constant changes in the conditions of the medium, exposing the yeasts to a series of simultaneous or sequential stresses and an efficient adaptation can lead to increased productivity and a improvement of their fermentative performance. In S. cerevisiae, adaptation involves an organized mobilization of genes called environmental stress response (ESR). Hsp12 is a protein belonging to families of heat shock proteins (HSPs) and this, in addition to maintaining the internal organization of the cell and increasing the flexibility of the cell wall and plasma membrane, is used as a stress reporter gene because its induction is in Largely through ESR already being used as a marker of stress status in yeast. Thus, the present work outlined a protocol of consecutive stress studies in order to evaluate morphological modifications and production of Hsp12 protein in S.cerevisiae. The results showed a similar variation of size of the mother cells and daughters in successive stresses compared to the growth of these cells in medium without stress addition. Cell cycle arrest was also a characteristic observed in both cells in consecutive stresses. The production of Hsp12 was higher in response to osmotic stress compared to oxidative and alcoholic stress in the isolated treatments, but the concentration of this protein in the latter two increased when the cell was exposed to consecutive stresses. This increase may be justified by the cross-protection of the acquired cell after contact with a high osmolarity solution. The difference in the results of the response to isolated and successive stresses shows that this methodology is more efficient to understand the behavior of the cell, because it is similar to the environment in the fermentation tanks.A levedura Saccharomyces cerevisiae desempenha um papel importante na indústria, devido a sua alta capacidade fermentativa. Durante a fermentação há mudanças constantes nas condições do meio, expondo as leveduras a uma série de estresses simultâneos ou sequenciais e uma adaptação eficiente pode levar a aumento da produtividade e um consequente aperfeiçoamento do seu desempenho fermentativo. Em S. cerevisiae, a adaptação envolve uma mobilização organizada de genes denominada resposta ao estresse ambiental (ESR). Hsp12 é uma proteína pertencente a famílias das proteínas de choque térmico (HSPs) e esta, além de manter a organização interna da célula e aumentar a flexibilidade da parede celular e membrana plasmática, é utilizada como gene repórter de estresse, pois sua indução é em grande parte através da ESR já sendo utilizada como um marcador do status de estresse em leveduras. Assim o presente trabalho delineou um protocolo de estudos de estresses consecutivos afim de avaliar modificações morfológicas e produção da proteína Hsp12 em S.cerevisiae. Os resultados mostraram uma variação semelhante de tamanho das células-mães e filhas nos estresses sucessivos comparada ao crescimento dessas células em meio sem adição de estresses. A parada no ciclo celular também foi uma característica observada em ambas as células em estresses consecutivos. A produção de Hsp12 foi maior em resposta ao estresse osmótico comparado aos estresses oxidativo e alcoólico nos tratamentos isolados, mas a concentração desta proteína nos dois últimos foi aumentada quando a célula foi exposta aos estresses consecutivos. Esse aumento pode ser justificado pela proteção cruzada da célula adquirida após o contato com uma solução com alta osmolaridade. A diferença nos resultados da resposta a estresses isolados e sucessivos constata que esta metodologia é mais eficaz para entender o comportamento da célula, pois se assemelha ao ambiente nas dornas de fermentação.Texthttp://repositorio.ufes.br/handle/10/7103porUniversidade Federal do Espírito SantoMestrado em BiotecnologiaPrograma de Pós-Graduação em BiotecnologiaUFESBRCentro de Ciências da SaúdeConsecutive stressesCross-protectionStress toleranceSaccharomyces cerevisiaeHsp12Estresses consecutivosProteção cruzadaTolerância a estresseResposta a estresses consecutivos em Saccharomyces cerevisiaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_10830_Dissertação_Ane Catarine Tosi Costa.pdfapplication/pdf1708151http://repositorio.ufes.br/bitstreams/2e45fb30-f894-44da-b67f-8c57415613b6/download864bf59fdffed1b6179cb9c87c5f7593MD5110/71032024-07-16 17:05:30.764oai:repositorio.ufes.br:10/7103http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T17:54:30.095339Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Resposta a estresses consecutivos em Saccharomyces cerevisiae
title Resposta a estresses consecutivos em Saccharomyces cerevisiae
spellingShingle Resposta a estresses consecutivos em Saccharomyces cerevisiae
Costa, Ane Catarine Tosi
Consecutive stresses
Cross-protection
Stress tolerance
Saccharomyces cerevisiae
Hsp12
Estresses consecutivos
Proteção cruzada
Tolerância a estresse
title_short Resposta a estresses consecutivos em Saccharomyces cerevisiae
title_full Resposta a estresses consecutivos em Saccharomyces cerevisiae
title_fullStr Resposta a estresses consecutivos em Saccharomyces cerevisiae
title_full_unstemmed Resposta a estresses consecutivos em Saccharomyces cerevisiae
title_sort Resposta a estresses consecutivos em Saccharomyces cerevisiae
author Costa, Ane Catarine Tosi
author_facet Costa, Ane Catarine Tosi
author_role author
dc.contributor.advisor-co1.fl_str_mv Fernandes, Antônio Alberto Ribeiro
dc.contributor.advisor1.fl_str_mv Fernandes, Patrícia Machado Bueno
dc.contributor.author.fl_str_mv Costa, Ane Catarine Tosi
dc.contributor.referee1.fl_str_mv Ventura, José Aires
dc.contributor.referee2.fl_str_mv Santos, Alexandre Martins Costa
contributor_str_mv Fernandes, Antônio Alberto Ribeiro
Fernandes, Patrícia Machado Bueno
Ventura, José Aires
Santos, Alexandre Martins Costa
dc.subject.eng.fl_str_mv Consecutive stresses
Cross-protection
Stress tolerance
topic Consecutive stresses
Cross-protection
Stress tolerance
Saccharomyces cerevisiae
Hsp12
Estresses consecutivos
Proteção cruzada
Tolerância a estresse
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
Hsp12
Estresses consecutivos
Proteção cruzada
Tolerância a estresse
description The yeast Saccharomyces cerevisiae yeast plays an important role in industry due to its high fermentative capacity. During fermentation, there are constant changes in the conditions of the medium, exposing the yeasts to a series of simultaneous or sequential stresses and an efficient adaptation can lead to increased productivity and a improvement of their fermentative performance. In S. cerevisiae, adaptation involves an organized mobilization of genes called environmental stress response (ESR). Hsp12 is a protein belonging to families of heat shock proteins (HSPs) and this, in addition to maintaining the internal organization of the cell and increasing the flexibility of the cell wall and plasma membrane, is used as a stress reporter gene because its induction is in Largely through ESR already being used as a marker of stress status in yeast. Thus, the present work outlined a protocol of consecutive stress studies in order to evaluate morphological modifications and production of Hsp12 protein in S.cerevisiae. The results showed a similar variation of size of the mother cells and daughters in successive stresses compared to the growth of these cells in medium without stress addition. Cell cycle arrest was also a characteristic observed in both cells in consecutive stresses. The production of Hsp12 was higher in response to osmotic stress compared to oxidative and alcoholic stress in the isolated treatments, but the concentration of this protein in the latter two increased when the cell was exposed to consecutive stresses. This increase may be justified by the cross-protection of the acquired cell after contact with a high osmolarity solution. The difference in the results of the response to isolated and successive stresses shows that this methodology is more efficient to understand the behavior of the cell, because it is similar to the environment in the fermentation tanks.
publishDate 2017
dc.date.issued.fl_str_mv 2017-03-09
dc.date.accessioned.fl_str_mv 2018-08-01T21:35:00Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T21:35:00Z
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dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Biotecnologia
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biotecnologia
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Biotecnologia
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