Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri

Detalhes bibliográficos
Autor(a) principal: Lemos, Pedro Henrique
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/7988
Resumo: The scorpionfish Scorpaena plumieri is one the most common venomous fish found along the Brazilian coast and is often involved in human accidents. Its venomous stings are covered by an integumentary sheath coated by a skin mucus layer, which may be carried along with the venom into the wound caused by the sting. This led us to suppose that the local and systemic manifestations induced by S. plumieri in accidents and experimental animals are combined effects of substances secreted by venom glands and epidermal cells. Therefore, the aim of this work was to continue the study S. plumieri venom (SpV) and initiate the physio-pharmacological and biochemical characterization of the skin mucus of this fish (SpSM), which has never been explored. In addition, a proteomic approach was employed to unravel and compare the complex composition of these two venoms. Both SpV and SpSM exhibit proteolytic activity upon gelatin and fibrinogen, albeit the hydrolysis was slow and non-specific. Hyaluronidase, cardiovascular, inflammatory and hemolytic activities were found only for SpV, evidencing the exclusive production of toxins (cytolysin and hyaluronidase) by the venom glands. The hemolysis seems to result from a direct action of venom constituents on erythrocytes membrane, since it lacks phospholipase A2 activity. Although devoid of hemolytic activity, SpSM was able to agglutinate rabbit erythrocytes (MHC 2.5mg/mL) suggesting the presence of carbohydrate-recognition proteins (lectins) in the mucus. Mass spectrometry analysis of the main fractions eluted from the two-dimensional liquid chromatography (gel filtration and reversed phase) revealed exclusive molecular species for SpV (533 = 36%) and SpSM (416 = 28%); 269 (18%) were found in both venoms. It was observed a predominance of mass lower than 10KDa in both SpV and SpSM (69% and 93%, respectively). The data here presented suggest that SpSM does not exhibit components capable to trigger relevant envenomation reactions. Nevertheless, together with SpV, it is a rich source of bioactive compounds, specially proteins and peptides
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spelling Andrich, FilipeFigueiredo, Suely Gomes deLemos, Pedro HenriqueSantos, Daniel Moreira dosRodrigues, Lívia Carla de Melo2018-08-01T22:58:45Z2018-08-012018-08-01T22:58:45Z2013-10-25The scorpionfish Scorpaena plumieri is one the most common venomous fish found along the Brazilian coast and is often involved in human accidents. Its venomous stings are covered by an integumentary sheath coated by a skin mucus layer, which may be carried along with the venom into the wound caused by the sting. This led us to suppose that the local and systemic manifestations induced by S. plumieri in accidents and experimental animals are combined effects of substances secreted by venom glands and epidermal cells. Therefore, the aim of this work was to continue the study S. plumieri venom (SpV) and initiate the physio-pharmacological and biochemical characterization of the skin mucus of this fish (SpSM), which has never been explored. In addition, a proteomic approach was employed to unravel and compare the complex composition of these two venoms. Both SpV and SpSM exhibit proteolytic activity upon gelatin and fibrinogen, albeit the hydrolysis was slow and non-specific. Hyaluronidase, cardiovascular, inflammatory and hemolytic activities were found only for SpV, evidencing the exclusive production of toxins (cytolysin and hyaluronidase) by the venom glands. The hemolysis seems to result from a direct action of venom constituents on erythrocytes membrane, since it lacks phospholipase A2 activity. Although devoid of hemolytic activity, SpSM was able to agglutinate rabbit erythrocytes (MHC 2.5mg/mL) suggesting the presence of carbohydrate-recognition proteins (lectins) in the mucus. Mass spectrometry analysis of the main fractions eluted from the two-dimensional liquid chromatography (gel filtration and reversed phase) revealed exclusive molecular species for SpV (533 = 36%) and SpSM (416 = 28%); 269 (18%) were found in both venoms. It was observed a predominance of mass lower than 10KDa in both SpV and SpSM (69% and 93%, respectively). The data here presented suggest that SpSM does not exhibit components capable to trigger relevant envenomation reactions. Nevertheless, together with SpV, it is a rich source of bioactive compounds, specially proteins and peptidesEspécimes do peixe-escorpião Scorpaena plumieri são comumente encontrados ao longo da costa brasileira e frequentemente causam envenenamentos em seres humanos. Seus espinhos são revestidos por uma bainha tegumentar, cobertas por uma camada de muco cutâneo que pode ser inoculado na ferida causada pelo espinho. Isso nos levou a supor que as manifestações locais e sistêmicas induzidas por S. plumieri possam resultar da ação sinérgica de substâncias secretadas pelas glândulas de peçonha e pelas células epidérmicas. Portanto, o objetivo deste trabalho foi dar continuidade ao estudo da peçonha de S. plumieri (SpV) e iniciar a caracterização físio-farmacológica e bioquímica do muco da pele deste peixe (SpSM), o qual nunca foi explorado. Além disso, a análise proteômica foi utilizada para desvendar e comparar a composição complexa destes dois venenos. Ambos SpV e SpSM exibem atividade proteolítica sobre gelatina e fibrinogênio, embora apresente hidrólise lenta e não-específica. Atividades hialuronidásica, cardiovascular, inflamatória e hemolítica foram encontradas somente para SpV, evidenciando a exclusiva produção de toxinas (citolisina e hialuronidase) pelas glândulas de peçonha. A hemólise parece resultar de uma ação direta de constituintes da peçonha em membranas de eritrócitos, uma vez que SpV não exibe atividade fosfolipásica do tipo A2. Embora desprovido de atividade hemolítica, SpSM foi capaz de aglutinar eritrócitos de coelho (MHC 2,5 mg/mL), o que sugere a presença de proteínas de reconhecimento de carboidratos (lectinas) no muco. A análise de espectrometria de massas das principais frações eluídas a partir da cromatografia líquida bidimensional (filtração em gel e de fase reversa) revelaram espécies moleculares exclusivas para SpV (533 = 36%) e SpSM (416 = 28%) e 269 (18%) encontradas em ambos os venenos. Foi observada uma predominância de massas inferiores a 10KDa em ambas as amostras SpV e SpSM (69% e 93%, respectivamente). Os dados obtidos sugerem que SpSM não apresenta componentes capazes de desencadear importantes reações no envenenamento, embora, juntamente com SpV, possa ser considerado uma rica fonte de compostos bioativos, especialmente proteínas e peptídeos.Texthttp://repositorio.ufes.br/handle/10/7988porUniversidade Federal do Espírito SantoMestrado em Ciências FisiológicasPrograma de Pós-Graduação em Ciências FisiológicasUFESBRCentro de Ciências da SaúdeFish venomSkin mucusBiological activitiesProteomic analysisPeçonha de peixeScorpaena plumieriMuco da peleAtividades biológicasAnálise proteômicaFisiologia612Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieriinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_6935_Dissertação Pedro Henrique Lemos.pdfapplication/pdf1506773http://repositorio.ufes.br/bitstreams/3bcefa28-24cf-4048-b1dd-dbc7597cda62/downloada19a43ee7169619d9db1e40dcebef91dMD5110/79882024-07-16 17:10:13.914oai:repositorio.ufes.br:10/7988http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T17:57:08.030492Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
title Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
spellingShingle Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
Lemos, Pedro Henrique
Fish venom
Skin mucus
Biological activities
Proteomic analysis
Peçonha de peixe
Scorpaena plumieri
Muco da pele
Atividades biológicas
Análise proteômica
Fisiologia
612
title_short Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
title_full Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
title_fullStr Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
title_full_unstemmed Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
title_sort Estudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri
author Lemos, Pedro Henrique
author_facet Lemos, Pedro Henrique
author_role author
dc.contributor.advisor-co1.fl_str_mv Andrich, Filipe
dc.contributor.advisor1.fl_str_mv Figueiredo, Suely Gomes de
dc.contributor.author.fl_str_mv Lemos, Pedro Henrique
dc.contributor.referee1.fl_str_mv Santos, Daniel Moreira dos
dc.contributor.referee2.fl_str_mv Rodrigues, Lívia Carla de Melo
contributor_str_mv Andrich, Filipe
Figueiredo, Suely Gomes de
Santos, Daniel Moreira dos
Rodrigues, Lívia Carla de Melo
dc.subject.eng.fl_str_mv Fish venom
Skin mucus
Biological activities
Proteomic analysis
topic Fish venom
Skin mucus
Biological activities
Proteomic analysis
Peçonha de peixe
Scorpaena plumieri
Muco da pele
Atividades biológicas
Análise proteômica
Fisiologia
612
dc.subject.por.fl_str_mv Peçonha de peixe
Scorpaena plumieri
Muco da pele
Atividades biológicas
Análise proteômica
dc.subject.cnpq.fl_str_mv Fisiologia
dc.subject.udc.none.fl_str_mv 612
description The scorpionfish Scorpaena plumieri is one the most common venomous fish found along the Brazilian coast and is often involved in human accidents. Its venomous stings are covered by an integumentary sheath coated by a skin mucus layer, which may be carried along with the venom into the wound caused by the sting. This led us to suppose that the local and systemic manifestations induced by S. plumieri in accidents and experimental animals are combined effects of substances secreted by venom glands and epidermal cells. Therefore, the aim of this work was to continue the study S. plumieri venom (SpV) and initiate the physio-pharmacological and biochemical characterization of the skin mucus of this fish (SpSM), which has never been explored. In addition, a proteomic approach was employed to unravel and compare the complex composition of these two venoms. Both SpV and SpSM exhibit proteolytic activity upon gelatin and fibrinogen, albeit the hydrolysis was slow and non-specific. Hyaluronidase, cardiovascular, inflammatory and hemolytic activities were found only for SpV, evidencing the exclusive production of toxins (cytolysin and hyaluronidase) by the venom glands. The hemolysis seems to result from a direct action of venom constituents on erythrocytes membrane, since it lacks phospholipase A2 activity. Although devoid of hemolytic activity, SpSM was able to agglutinate rabbit erythrocytes (MHC 2.5mg/mL) suggesting the presence of carbohydrate-recognition proteins (lectins) in the mucus. Mass spectrometry analysis of the main fractions eluted from the two-dimensional liquid chromatography (gel filtration and reversed phase) revealed exclusive molecular species for SpV (533 = 36%) and SpSM (416 = 28%); 269 (18%) were found in both venoms. It was observed a predominance of mass lower than 10KDa in both SpV and SpSM (69% and 93%, respectively). The data here presented suggest that SpSM does not exhibit components capable to trigger relevant envenomation reactions. Nevertheless, together with SpV, it is a rich source of bioactive compounds, specially proteins and peptides
publishDate 2013
dc.date.issued.fl_str_mv 2013-10-25
dc.date.accessioned.fl_str_mv 2018-08-01T22:58:45Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T22:58:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufes.br/handle/10/7988
url http://repositorio.ufes.br/handle/10/7988
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Text
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Fisiológicas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Fisiológicas
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Fisiológicas
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institution UFES
reponame_str Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
collection Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
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