Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/18725 |
Resumo: | The infection by Helicobacter pylori (H.pylori) can be related to the occurrence of carcinomas and MALT lymphomas, while the latent infection by Epstein-Barr virus (EBV) is associated both to gastric epithelial malignant neoplasias as to lymphomatous gastric epithelial neoplasias. The purpose of this study was to perform a systematic and global histopathological analysis of gastric biopsies from children submitted to upper digestive endoscopy, with recurrent abdominal pain, in Antonio Pedro University Hospital/UFF and to study the association between lymphoid proliferation and H.pylori and EBV. The gastric biopsies (n=142) from children up to 12 years old (n=129; 52% male; age 8±3 years) from January 2002 to December 2004 were reviewed and the slides were stained with Giemsa and Whartin-Starry for identification of H.pylori and immunostains for EBV (anti-LMP1) and T-lymphocytes CD8+ were used. Under light microscopy, 63% presented chronic gastritis and 30% infection by H.pylori. Forty-eight percent of children with chronic gastritis had lymphoid proliferation, but 15 among these were negative for H.pylori. None of the children was positive for EBV and 83% of them presented up to five T CD8+ lymphocytes per 100 superficial epithelial cells. H.pylori positive children were older than the negative ones (9.1±2.8 vs. 7.1±3.3 years; P<0,001) and the presence of lymphoid response was more frequently found in children with H.pylori (80% vs. 15%; P <0,001). In conclusion, EBV is not related to lymphoid response in children gastric biopsies. However, this lymphoid proliferation is associated with H.pylori infection in 48% of children with chronic gastritis. Five out of 15 children with lymphoid proliferation but negative for H.pylori, had been previously treated for this bacteria, suggesting that this response could be post-treatment sequelae. The remaining 10 biopsies could represent post-treatment sequelae from other systemic infections. |
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Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-BarrGastrite crônicaHelicobacter pyloriResposta linfóideVírus Epstein-BarrMEDICINAANATOMIA PATOLÓGICAGastriteHerpesvírus humano 4Infecção por helicobacterChronic gastritisHelicobacter pyloriLymphoid proliferationEpstein-Barr virusCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAThe infection by Helicobacter pylori (H.pylori) can be related to the occurrence of carcinomas and MALT lymphomas, while the latent infection by Epstein-Barr virus (EBV) is associated both to gastric epithelial malignant neoplasias as to lymphomatous gastric epithelial neoplasias. The purpose of this study was to perform a systematic and global histopathological analysis of gastric biopsies from children submitted to upper digestive endoscopy, with recurrent abdominal pain, in Antonio Pedro University Hospital/UFF and to study the association between lymphoid proliferation and H.pylori and EBV. The gastric biopsies (n=142) from children up to 12 years old (n=129; 52% male; age 8±3 years) from January 2002 to December 2004 were reviewed and the slides were stained with Giemsa and Whartin-Starry for identification of H.pylori and immunostains for EBV (anti-LMP1) and T-lymphocytes CD8+ were used. Under light microscopy, 63% presented chronic gastritis and 30% infection by H.pylori. Forty-eight percent of children with chronic gastritis had lymphoid proliferation, but 15 among these were negative for H.pylori. None of the children was positive for EBV and 83% of them presented up to five T CD8+ lymphocytes per 100 superficial epithelial cells. H.pylori positive children were older than the negative ones (9.1±2.8 vs. 7.1±3.3 years; P<0,001) and the presence of lymphoid response was more frequently found in children with H.pylori (80% vs. 15%; P <0,001). In conclusion, EBV is not related to lymphoid response in children gastric biopsies. However, this lymphoid proliferation is associated with H.pylori infection in 48% of children with chronic gastritis. Five out of 15 children with lymphoid proliferation but negative for H.pylori, had been previously treated for this bacteria, suggesting that this response could be post-treatment sequelae. The remaining 10 biopsies could represent post-treatment sequelae from other systemic infections.A infecção gástrica pelo Helicobacter pylori (H.pylori) pode estar relacionada ao aparecimento de carcinomas e de linfomas MALT e a infecção latente pelo vírus Epstein-Barr (EBV) está associada tanto ao desenvolvimento de neoplasias malignas epiteliais gástricas usuais, quanto a neoplasias epiteliais com estroma linfomatoso. O objetivo deste trabalho foi realizar uma análise histopatológica sistemática e global das biópsias gástricas de crianças submetidas a endoscopia digestiva alta, com queixas de dor abdominal recorrente, no Hospital Universitário Antônio Pedro/UFF e estudar a possível associação da resposta linfóide com Helicobacter pylori e com vírus Epstein-Barr. Foram revistas as biopsias gástricas (n=142) realizadas entre janeiro de 2002 a dezembro de 2004 de crianças com até 12 anos (n=129; 52% masculino; idade 8±3 anos) e os fragmentos foram submetidos às colorações de Giemsa e Whartin-Starry para pesquisa de H.pylori, à imuno-histoquímica para pesquisa de EBV (anti-LMP1) e pesquisa de linfócitos T CD8+. À microscopia, 63% apresentaram gastrite crônica e 30% infecção pelo H.pylori. Dentre as crianças com gastrite crônica, 48% apresentaram resposta linfóide, sendo que apenas 15 dessas não tinham positividade para H.pylori. Nenhuma das crianças apresentou positividade para EBV e 83% delas exibiu até 5 linfócitos T CD8+ por 100 células epiteliais superficiais. A idade das crianças positivas para H.pylori foi maior do que as negativas (9,1±2,8 vs. 7,1±3,3 anos; P<0,001) e a presença de resposta linfóide foi mais freqüente nas crianças com H.pylori (80% vs. 15%; P <0,001). Em conclusão, o EBV não está relacionado a resposta linfóide em biopsias gástricas de crianças, entretanto este afluxo linfóide está associado a positividade do H. pylori em 48% das crianças com gastrite crônica. De quinze crianças com reposta linfóide e H.pylori negativo, cinco foram tratadas para erradicação do bacilo, podendo ser esta resposta uma seqüela pós-tratamento. As dez biopsias restantes sugerem tratar-se também de resolução/seqüela pós-tratamento para outras infecções sistêmicas.Programa de Pós-graduação em PatologiaPatologiaLopes, Vânia SilamiCPF:99985743122http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793225Y6Dias, Eliane PedraCPF:77790588753http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786983U3&dataRevisao=nullGuedes, Lélia Paiva eCPF:49579486522Moraes, Heleno Pinto deCPF:27895653768Herdy, Gesmar Volga HaddadCPF:98495468122http://lattes.cnpq.br/3333782223605106Carvalho, Angela Cristina Gouvêa2021-03-10T20:45:24Z2010-02-092021-03-10T20:45:24Z2006-12-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18725porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:45:24Zoai:app.uff.br:1/18725Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202021-03-10T20:45:24Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
title |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
spellingShingle |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr Carvalho, Angela Cristina Gouvêa Gastrite crônica Helicobacter pylori Resposta linfóide Vírus Epstein-Barr MEDICINA ANATOMIA PATOLÓGICA Gastrite Herpesvírus humano 4 Infecção por helicobacter Chronic gastritis Helicobacter pylori Lymphoid proliferation Epstein-Barr virus CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
title_full |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
title_fullStr |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
title_full_unstemmed |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
title_sort |
Resposta linfóide em biópsias gástricas de crianças do Hospital Universitário Antonio Pedro: associação com Helicobacter pylori e com vírus Epstein-Barr |
author |
Carvalho, Angela Cristina Gouvêa |
author_facet |
Carvalho, Angela Cristina Gouvêa |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lopes, Vânia Silami CPF:99985743122 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793225Y6 Dias, Eliane Pedra CPF:77790588753 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786983U3&dataRevisao=null Guedes, Lélia Paiva e CPF:49579486522 Moraes, Heleno Pinto de CPF:27895653768 Herdy, Gesmar Volga Haddad CPF:98495468122 http://lattes.cnpq.br/3333782223605106 |
dc.contributor.author.fl_str_mv |
Carvalho, Angela Cristina Gouvêa |
dc.subject.por.fl_str_mv |
Gastrite crônica Helicobacter pylori Resposta linfóide Vírus Epstein-Barr MEDICINA ANATOMIA PATOLÓGICA Gastrite Herpesvírus humano 4 Infecção por helicobacter Chronic gastritis Helicobacter pylori Lymphoid proliferation Epstein-Barr virus CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Gastrite crônica Helicobacter pylori Resposta linfóide Vírus Epstein-Barr MEDICINA ANATOMIA PATOLÓGICA Gastrite Herpesvírus humano 4 Infecção por helicobacter Chronic gastritis Helicobacter pylori Lymphoid proliferation Epstein-Barr virus CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
The infection by Helicobacter pylori (H.pylori) can be related to the occurrence of carcinomas and MALT lymphomas, while the latent infection by Epstein-Barr virus (EBV) is associated both to gastric epithelial malignant neoplasias as to lymphomatous gastric epithelial neoplasias. The purpose of this study was to perform a systematic and global histopathological analysis of gastric biopsies from children submitted to upper digestive endoscopy, with recurrent abdominal pain, in Antonio Pedro University Hospital/UFF and to study the association between lymphoid proliferation and H.pylori and EBV. The gastric biopsies (n=142) from children up to 12 years old (n=129; 52% male; age 8±3 years) from January 2002 to December 2004 were reviewed and the slides were stained with Giemsa and Whartin-Starry for identification of H.pylori and immunostains for EBV (anti-LMP1) and T-lymphocytes CD8+ were used. Under light microscopy, 63% presented chronic gastritis and 30% infection by H.pylori. Forty-eight percent of children with chronic gastritis had lymphoid proliferation, but 15 among these were negative for H.pylori. None of the children was positive for EBV and 83% of them presented up to five T CD8+ lymphocytes per 100 superficial epithelial cells. H.pylori positive children were older than the negative ones (9.1±2.8 vs. 7.1±3.3 years; P<0,001) and the presence of lymphoid response was more frequently found in children with H.pylori (80% vs. 15%; P <0,001). In conclusion, EBV is not related to lymphoid response in children gastric biopsies. However, this lymphoid proliferation is associated with H.pylori infection in 48% of children with chronic gastritis. Five out of 15 children with lymphoid proliferation but negative for H.pylori, had been previously treated for this bacteria, suggesting that this response could be post-treatment sequelae. The remaining 10 biopsies could represent post-treatment sequelae from other systemic infections. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-28 2010-02-09 2021-03-10T20:45:24Z 2021-03-10T20:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/18725 |
url |
https://app.uff.br/riuff/handle/1/18725 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
_version_ |
1802135313769824256 |