NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains

Detalhes bibliográficos
Autor(a) principal: Nóbrega, Pedro Ivo Lucena da
Data de Publicação: 2016
Outros Autores: Faget, Douglas V., Pachulec, Emilia, Robaina, Marcela C., Klumb, Claudete E., Robbs, Bruno K., Viola, João P. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
Texto Completo: http://app.uff.br/riuff/handle/1/28833
Resumo: Sim
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spelling NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domainsNFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domainsIsoformas de proteínasFatores de Transcrição NFATCSimThe NFAT (nuclear factor of activated T cells) family of transcription factors is composed of four calcium-responsive proteins (NFAT1 to -4). The NFAT2 (also called NFATc1) gene encodes the isoforms NFAT2 and NFAT2 that result mainly from alternative initiation exons that provide two different N-terminal transactivation domains. However, the specific roles of the NFAT2 isoforms in cell physiology remain unclear. Because previous studies have shown oncogenic potential for NFAT2, this study emphasized the role of the NFAT2 isoforms in cell transformation. Here, we show that a constitutively active form of NFAT2 (CANFAT2 ) and CA-NFAT2 distinctly control death and transformation in NIH 3T3 cells. While CA-NFAT2 strongly induces cell transformation, CA-NFAT2 leads to reduced cell proliferation and intense cell death through the upregulation of tumor necrosis factor alpha (TNF- ). CA-NFAT2 also increases cell death and upregulates Fas ligand (FasL) and TNF- in CD4 T cells. Furthermore, we demonstrate that differential roles of NFAT2 isoforms in NIH 3T3 cells depend on the N-terminal domain, where the NFAT2 -specific N-terminal acidic motif is necessary to induce cell death. Interestingly, the NFAT2 isoform is upregulated in Burkitt lymphomas, suggesting an isoform-specific involvement of NFAT2 in cancer development. Finally, our data suggest that alternative N-terminal domains of NFAT2 could provide differential mechanisms for the control of cellular functions.Taylor and FrancisNiterói, RJ2023-05-16T12:00:52Z2023-05-16T12:00:52Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNÓBREGA, Pedro Ivo Lucena da et al. NFAT2 isoforms differentially regulate gene expression, cell death,dand transformation through alternative n-terminal domains. Molecular and Cellular Biology, v. 36, n. 1, p. 119-131, Jan. 2016. Disponível em: https://doi.org/10.1128/MCB.00501-15http://app.uff.br/riuff/handle/1/28833Aluno de Graduaçãodoi.org/10.1128/MCB.00501-15MOLECULAR AND CELLULAR BIOLOGY. London: Taylor and Francis, 1981-2023. Mensal. ISSN: 1098-5549CC-BY-SAinfo:eu-repo/semantics/openAccessNóbrega, Pedro Ivo Lucena daFaget, Douglas V.Pachulec, EmiliaRobaina, Marcela C.Klumb, Claudete E.Robbs, Bruno K.Viola, João P. B.engreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2023-05-17T21:01:12Zoai:app.uff.br:1/28833Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T10:57:09.362079Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false
dc.title.none.fl_str_mv NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
title NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
spellingShingle NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
Nóbrega, Pedro Ivo Lucena da
Isoformas de proteínas
Fatores de Transcrição NFATC
title_short NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
title_full NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
title_fullStr NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
title_full_unstemmed NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
title_sort NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains
author Nóbrega, Pedro Ivo Lucena da
author_facet Nóbrega, Pedro Ivo Lucena da
Faget, Douglas V.
Pachulec, Emilia
Robaina, Marcela C.
Klumb, Claudete E.
Robbs, Bruno K.
Viola, João P. B.
author_role author
author2 Faget, Douglas V.
Pachulec, Emilia
Robaina, Marcela C.
Klumb, Claudete E.
Robbs, Bruno K.
Viola, João P. B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nóbrega, Pedro Ivo Lucena da
Faget, Douglas V.
Pachulec, Emilia
Robaina, Marcela C.
Klumb, Claudete E.
Robbs, Bruno K.
Viola, João P. B.
dc.subject.por.fl_str_mv Isoformas de proteínas
Fatores de Transcrição NFATC
topic Isoformas de proteínas
Fatores de Transcrição NFATC
description Sim
publishDate 2016
dc.date.none.fl_str_mv 2016
2023-05-16T12:00:52Z
2023-05-16T12:00:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NÓBREGA, Pedro Ivo Lucena da et al. NFAT2 isoforms differentially regulate gene expression, cell death,dand transformation through alternative n-terminal domains. Molecular and Cellular Biology, v. 36, n. 1, p. 119-131, Jan. 2016. Disponível em: https://doi.org/10.1128/MCB.00501-15
http://app.uff.br/riuff/handle/1/28833
Aluno de Graduação
doi.org/10.1128/MCB.00501-15
identifier_str_mv NÓBREGA, Pedro Ivo Lucena da et al. NFAT2 isoforms differentially regulate gene expression, cell death,dand transformation through alternative n-terminal domains. Molecular and Cellular Biology, v. 36, n. 1, p. 119-131, Jan. 2016. Disponível em: https://doi.org/10.1128/MCB.00501-15
Aluno de Graduação
doi.org/10.1128/MCB.00501-15
url http://app.uff.br/riuff/handle/1/28833
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv MOLECULAR AND CELLULAR BIOLOGY. London: Taylor and Francis, 1981-2023. Mensal. ISSN: 1098-5549
dc.rights.driver.fl_str_mv CC-BY-SA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC-BY-SA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
Niterói, RJ
publisher.none.fl_str_mv Taylor and Francis
Niterói, RJ
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)
instname:Universidade Federal Fluminense (UFF)
instacron:UFF
instname_str Universidade Federal Fluminense (UFF)
instacron_str UFF
institution UFF
reponame_str Repositório Institucional da Universidade Federal Fluminense (RIUFF)
collection Repositório Institucional da Universidade Federal Fluminense (RIUFF)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)
repository.mail.fl_str_mv riuff@id.uff.br
_version_ 1811823617245184000

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