Detecção e caracterização molecular de norovírus em casos de gastroenterite aguda ocorridos na região do médio Paraíba, Estado do Rio de Janeiro, 2005.

• Autor(a) principal: Ferreira, Mônica Simões Rocha
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/18446
• topic: Gastroenterite; Norovírus; MEDICINA; CIÊNCIAS MÉDICAS; Gastroenterite aguda; Epidemiologia molecular; CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA

In March 2005, the Epidemiological Surveillance Service of Resende, municipality of the Middle Paraiba Valley, State of Rio de Janeiro, reported a sudden spontaneous occurrence of acute gastroenteritis cases in children in a public day care center. The follow investigation showed that children s relative were also affected. From May to June, two other municipalities, Piraí and Rio Claro, also localized in the Middle Paraiba Valley, reported gastroenteritis outbreaks or sporadic cases. A total of 50 fecal samples were collected from March to June 2005. Since bacterial and parasitic investigations were negative in those samples, we performed virus investigation in those outbreaks and sporadic cases of acute gastroenteritis. Polyacrylamide gel electrophoresis for rotaviruses, enzyme immunoassay for adenovirus and rotavirus, reverse transcription-polymerase chain reaction (RT-PCR) were performed to investigate the presence of noroviruses (NoV) and astroviruses. Also, a quantitative TaqMan real time PCR for NoV was performed to detect, quantified samples and to compare these results with those obtained by conventional RT-PCR. NoV was detected in 33 out of 50 (66%) samples showing an agreement of 100% between both methodologies. Partial nucleotide sequence analysis of the genome sequence for the capsid showed that the circulating strain belonged to genogroup II and genotype 4. The results highlight the role of NoV infection in children and adults in outbreaks as well as in sporadic cases of acute gastroenteritis.