Diagnóstico da infecção por amebídeos através de técnicas morfológicas, imunológicas e moleculares em microrregião de Ilhéus - Itabuna, Bahia, Brasil

• Autor(a) principal: Martins, Luci Ana Fernandes
• Data de Publicação: 2010
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/20225
• topic: Amebíase; Entamoeba; Parasitas intestinais; MEDICINA; PATOLOGIA; Doenças parasitárias; Amoebiasis; Entamoeba; Intestinal parasites; CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA

The Entamoeba gender possesses several species, hindering the diagnosis of amebiasis for morphologic likeness among protozoa, what carts in erroneous diagnosis and unnecessary treatment of many patients. Because of this, new techniques are being developed with the objective of differentiate those species. The present work has as objectives to evaluate the hygienic and sanitary conditions and the frequency of intestinal parasitosis on the studied population, identify and differentiate through immunological and molecular techniques the species of Entamoeba histolytica/ Entamoeba dispar complex and identify through morphological techniques the specie of Entamoeba hartmanni in residents of a micro region of Ilhéus-Itabuna. Thirty eight families participated in the study, having been collected 97 people's fecal samples. In the parasitological examination of feces, were used direct methods (Paratest) and the sedimentation method for centrifugation (modified Ritchie). The coproantigen research was accomplished through the commercial imunnoenzimactic method (ELISA, E. histolytica II-TECHLAB Inc. Blacksburg, VA, USA). In differentiation of species of E.histolytica/ E. dispar complex, the polymerase chain reaction of Multiplex PCR was accomplished and the E. hartmanni specie was identified through morphometry on slides stained by ferric hematoxylin. The results are suggestive that hygienic and sanitary conditions at the settlement are not satisfactory, being observed through the parasitological examination of feces a global frequency of enteroparasitosis by the order of 65%. The evaluation of specific frequency shown Ascaris lumbricoides (29%); Trichuris trichiura (10%); Ancylostomids (8%); Strongyloides stercoralis (3%), Entamoeba coli (25%); Endolimax nana (25%); Blastocistis hominis (13%); Entamoeba histolytica/Entamoeba dispar (25%); Giardia intestinalis (3%); Iodameba butschlii (1%). The method of floating-centrifugation was most efficient than the direct method on parasites recovering. The coproantigen research was efficient on identification of Entamoeba histolytica, showing that E. dispar was the most frequent specie in the studied population. The method of ferric hematoxylin colour was an important tool to identify E. hartmanni. This study shows that through association of various techniques, it is possible to identify amoebiasis agent, differentiating it from the other enteric amoebas, contributing to epidemiologic studies and avoiding unnecessary treatment of patients infected by other amoebas than E. histolytica