Produção de biofilme, detecção dos genes icaAD, atlE e aap e resistência aos antimicrobianos em cepas de Staphylococcus epidermidis resistentes à meticilina (MRSE)

• Autor(a) principal: Araujo, Gabrielle Luck de
• Data de Publicação: 2004
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/18425
• topic: Biofilme; Genes icaAD, atlE e aap; Resistência a antimicrobianos; Staphylococcus epidermidis; MEDICINA; CIÊNCIAS MÉDICAS; Gene; Resistência a meticilina; Biofilm; CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA

Biofilm has been considered the major virulence factor of Staphylococcus epidermidis. The presence of ica operon and of other genes, including atlE and aap, seems to be important for its formation. However, there are controversies in relation to the genes considered essential for biofilm production. In the present work we aimed to study the expression of biofilm, the presence of icaAD, atlE and aap genes and antimicrobial resistance in strains of methicillin-resistant S. epidermidis (MRSE) obtained from hospitalized patients at the Antônio Pedro University Hospital (HUAP; 25 strains), healthy individuals of the community (35 strains) and individuals of a home care system (HC; 77 strains). For biofilm detection, cultures were inoculated in Trypticase Soy Broth supplemented with 1% glucose. The biofilm was formed on polystyrene microtitre plates and subsequently stained with violet crystal. The polymerase chain reaction (PCR) was used for detecting mecA (encoding for methicillin resistance) icaAD, atlE and aap genes. Of the hospital strains, six (24 %) were classified as non-adherent, nine (36 %) as weakly, five (20 %) moderately and other five (20 %) as strongly adherent; showing certain homogeneity in the biofilm expression profile. Of the community strains, 14 (40 %) were non-adherent, three (8 %) weakly, nine (26 %) moderately and nine (26 %) strongly adherent. From the 77 HC strains, 33 (43 %) were non-adherent, 12 (16 %) weakly, seven (9 %) moderately and 25 (32 %) strongly adherent. Of the total biofilm producer strains, 99 % carried icaAD, 86 % atlE and 82 % aap. In this study we detected significant difference in the biofilm production between hospital and non-hospital MRSE strains. Our data also showed correlation between the characteristic of strong biofilm formation and the concomitant presence of icaAD, atlE and aap genes. Finally, multiresistant strains of MRSE were associated with adherent pattern mostly; suggesting the biofilm environment may facilitate exchanges of antimicrobial resistant genes.