Contribuição ao estudo da microbiota bacteriana presente em vesícula biliar de bovinos abatidos em matadouro frigorífico sob inspeção sanitária

• Autor(a) principal: Dias, Francesca Silva
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/17960
• topic: Medicina veterinária; Inspeção de carne; Bovino; Vesicula biliar; Antibiótico; Bactérias; Resistência microbiana a drogas; Microbiologia; Antimicrobiano; Patógenos; Tecnologia de carnes; Gallbladder; Cattle; Resistance; Antimicrobial; Pathogens; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

The cattle have a resistant microbiote in the skin and digestive tract which are transferred to the carcass during slaughter. Because of that, more attention should be given to the gastrointestinal tract, especially to the gallbladder, which, even though presents adverse characteristics such as pH variations and high osmolarity, it is described as a potent habitat for several bacteria. This is because the microorganisms have been developing mechanisms to tolerate the bile, such as protein alterations which dominate the construction of the cell s membrane and maintenance of intracellular homalostasis. Thirty full gallbladders were collected of cattle slaughtered in slaughterhouses under sanitary inspection with the purpose to research bacteria present in the liquid and epithelium. The microorganisms in study were Heterophilic Aerobic Mesophis Bacteria (HAMB), genus Staphylococcus and Enterobacteria in agreement to, Brazil (2003); E. coli e Enterococcus spp. as Merck (2000) with appropriate modifications, and Salmonella spp. according to the methodology described by Pignato (1995). During research, an anti-microbial sensitivity test was also done for isolated strains of Salmonella and Staphylococcus genus. The isolation frequency of HAMB of Staphylococcus, Enterobacteria, E. coli, Enterococcus spp. and Salmonella spp genus was, respectively: 23.02%, 14.39%, 13.67%, 24.46%, 0% and 24.46%. Concerning both environments of the gallbladder, the isolation frequency of the microorganisms in the epithelium was 64.03%, and in the liquid 35.97%, with no statistical difference in Chi-Square test (p>0.05), but with significant difference between the population averages ascertained in the t Student test (p<0.05). In the anti-microbial sensitivity test, Salmonella spp. strains and Staphylococcus genus had the highest average of resistance to drugs in the biliary liquid and the sensitivity frequency of microorganisms by Fisher s exact test (p<0.05) to some drugs was greater when these bacteria were found in the biliary epithelium. For the Staphylococcus genus the antimicrobials of choice were: penicillin G, ceftriaxone, chloramphenicol and gentamicin, and for Salmonella spp.: aztreonam. However, in strain salmonella multidrug resistance occurred. The observation that the gallbladder supports a high frequency of several microorganisms brings us to the possible fact that the cattle might be a persistent carrier of pathogens of great importance to collective health and thus, the sanitary inspection services should review news procedures for the common technique of gallbladder compression to avoid bile spill over the liver surface.