Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/18723 |
Resumo: | The general objective of this work was to evaluate the fertility of male domestic and wild felids through andrological examination and testicular cytology using nonaspiration fine needle cytology. Twenty-five domestic cats (Felis catus, Linnaeus,1758), of various ages, presented for clinical evaluation at the University Veterinary Clinic were examined. Seven adult small wild felids were also examined: five oncillas (Leopardus tigrinus, Schreber, 1775), one Geoffroy s cat (Leopardus geoffroyi, d Orbign & Gervais, 1843), and one jaguarundi (Puma yagouaroundi, E. Geoffroyi, 1803), all belonging to the Fundação RIOZOO (RJ). All animals were submitted to a complete andrological examination and testicular cytology. The animals were kept under anesthesia throughout the whole procedure with the use of ketamine (5 mg/kg), xylazine (0.5 mg/kg) and atropine (0.04 mg/kg) as well as epidural anesthesia with 2% lidocaine without vasoconstrictor (6 mg/kg). Semen samples were collected using the electroejaculation method and examined regarding its macroscopic characteristics (volume, color and odor) as well as microscopic characteristics (motility, vigor and morphology). Following the examination, a testicular cytology using nonaspiration fine needle cytology was performed on both testicles. Testicular cytology revealed cells from the spermatic lineage as well as Sertoli cells. The color of the ejaculate varied from transparent to milky white, while odor showed no alterations and seminal pH varied between 6.0 and 7.0. Mean sperm motility was 60% in domestic cats and 80% in wild felids. Vigor varied between 0 and 5. The average and standard deviation primary defects observed were 22,57 ± 13,44% for domestic cats and 14,75 ± 4,13% for wild felids. The domestic cats secondary defects were 16,20 ± 6,82% and the wild 17,00 ± 6,27%.Mean total sperm defects observed were 38.78 ± 17,11% for domestic cats and 31.75 ± 8,50% for wild felids. Regarding the macroscopic evaluation of the testicles of domestic cats, 70% of the animals had oval-shaped testicles and in the microscopic evaluation, focal hemorrhaging was observed in 88% of cats and 10% of the seminiferous tubules were found to be underdeveloped. The Mann-Whitney test demonstrated that there was no significant difference (p>0.05) between the studied groups. It was concluded that 60% of domestic cats were fertile; within the wild felids, the jaguarondi was the only cat to present compromised reproductive capability; the anesthetic protocol used in this study was found to be efficient for andrological evaluation; there is no correlation between mensurated scrotal perimeter and seminal characteristics; the electroejaculation technique in felids allows for the collection of a good quality semen sample for evaluation; the clinical examination and seminal analysis were essential for fertility evaluation of the male individuals. Testicular cytology using nonaspiration fine needle cytology proved to be a fast, simple and efficient technique; testicular cytology is an important complementary method for the diagnosis of subfertility and infertility |
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Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae CarnívoraFelino domésticoSelvagemSêmenEletroejaculaçãoHistopatologiaCitologiaTestículoDomestic felineSalvageSemenElectroejaculationHistopathologyCytologyTestisCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAThe general objective of this work was to evaluate the fertility of male domestic and wild felids through andrological examination and testicular cytology using nonaspiration fine needle cytology. Twenty-five domestic cats (Felis catus, Linnaeus,1758), of various ages, presented for clinical evaluation at the University Veterinary Clinic were examined. Seven adult small wild felids were also examined: five oncillas (Leopardus tigrinus, Schreber, 1775), one Geoffroy s cat (Leopardus geoffroyi, d Orbign & Gervais, 1843), and one jaguarundi (Puma yagouaroundi, E. Geoffroyi, 1803), all belonging to the Fundação RIOZOO (RJ). All animals were submitted to a complete andrological examination and testicular cytology. The animals were kept under anesthesia throughout the whole procedure with the use of ketamine (5 mg/kg), xylazine (0.5 mg/kg) and atropine (0.04 mg/kg) as well as epidural anesthesia with 2% lidocaine without vasoconstrictor (6 mg/kg). Semen samples were collected using the electroejaculation method and examined regarding its macroscopic characteristics (volume, color and odor) as well as microscopic characteristics (motility, vigor and morphology). Following the examination, a testicular cytology using nonaspiration fine needle cytology was performed on both testicles. Testicular cytology revealed cells from the spermatic lineage as well as Sertoli cells. The color of the ejaculate varied from transparent to milky white, while odor showed no alterations and seminal pH varied between 6.0 and 7.0. Mean sperm motility was 60% in domestic cats and 80% in wild felids. Vigor varied between 0 and 5. The average and standard deviation primary defects observed were 22,57 ± 13,44% for domestic cats and 14,75 ± 4,13% for wild felids. The domestic cats secondary defects were 16,20 ± 6,82% and the wild 17,00 ± 6,27%.Mean total sperm defects observed were 38.78 ± 17,11% for domestic cats and 31.75 ± 8,50% for wild felids. Regarding the macroscopic evaluation of the testicles of domestic cats, 70% of the animals had oval-shaped testicles and in the microscopic evaluation, focal hemorrhaging was observed in 88% of cats and 10% of the seminiferous tubules were found to be underdeveloped. The Mann-Whitney test demonstrated that there was no significant difference (p>0.05) between the studied groups. It was concluded that 60% of domestic cats were fertile; within the wild felids, the jaguarondi was the only cat to present compromised reproductive capability; the anesthetic protocol used in this study was found to be efficient for andrological evaluation; there is no correlation between mensurated scrotal perimeter and seminal characteristics; the electroejaculation technique in felids allows for the collection of a good quality semen sample for evaluation; the clinical examination and seminal analysis were essential for fertility evaluation of the male individuals. Testicular cytology using nonaspiration fine needle cytology proved to be a fast, simple and efficient technique; testicular cytology is an important complementary method for the diagnosis of subfertility and infertilityO presente estudo foi realizado em 25 gatos domésticos (Felis catus, Linnaeus,1758), diversas idades, oriundos da Clínica Escola. Foram examinados sete pequenos felinos selvagens, adultos: cinco gatos-do-mato pequenos (Leopardus tigrinus, Schreber, 1775), um gato-do-mato grande (Leopardus geoffroyi, d Orbign & Gervais, 1843) e um gato jaguarondi (Puma yagouaroundi, E. Geoffroyi, 1803), da Fundação RIOZOO (RJ). O objetivo geral do trabalho foi avaliar a fertilidade (capacidade reprodutiva) de felinos domésticos e selvagens, através de exames andrológicos e citologia testicular, com punção por agulha fina sem aspiração. Foram realizados exame andrológico completo e citologia testicular. Durante os exames, estes foram anestesiados com ketamina (5 mg/kg), xilazina (0,5 mg/kg) e atropina (0,04 mg/kg), associada com lidocaína 2% sem vasoconstritor peridural (6 mg/kg). As amostras de sêmen foram coletadas por eletroejaculação e examinadas quanto às características macroscópicas (volume, coloração e odor) e microscópicas (motilidade, vigor e morfologia). Após os exames foi realizada a punção sem aspiração dos testículos. A maioria dos animais apresentou normalidade no exame clínico e na biometria testicular. Na citologia testicular foram encontradas células da linhagem espermática e Sertoli. A coloração dos ejaculados variou de transparente a branco leitoso, o odor não apresentou alterações e o pH do sêmen variou de 6,0 a 7,0. A média da motilidade observada nos felinos domésticos foi de 60,0 ± 29,91% e dos selvagens de 80,0 ± 12,65%. O vigor variou de 0 a 5. A média e o respectivo desvio padrão dos defeitos maiores observados nos felinos domésticos foram de 22,57 ± 13,44% e os dos selvagens de 14,75 ± 4,13%. Nos felinos domésticos os defeitos menores foram de 16,20 ± 6,82% e dos selvagens de 17,00 ± 6,27%. A média do total de defeitos nos felinos domésticos foi 38,78 ± 17,11% e nos selvagens foi 31,75 ± 8,50%. Na macroscopia dos felinos domésticos, os testículos apresentaram o formato ovalado em 70% e na microscopia 88% tinham hemorragias focais e 10% dos túbulos seminíferos apresentavam-se pouco desenvolvidos. No teste de Mann-Whitney não foi verificada diferença estatisticamente significativa (p>0,05) nas características avaliadas, não havendo diferenças significativas entre os felinos domésticos e selvagens. Conclui-se que, 60% dos felinos domésticos são férteis; nos selvagens somente o gato jaguarondi, teve sua capacidade reprodutiva comprometida; o protocolo anestésico utilizado foi bastante eficaz em todos os procedimentos. Não houve correlação entre o perímetro escrotal medido e as características seminais; a técnica de eletroejaculação nos felinos permitiu obter ejaculado de boa qualidade; o exame clínico e a análise seminal foram essenciais para avaliação da fertilidade do macho. A punção sem aspiração mostrou-se ser uma técnica simples, rápida e eficiente; a citologia testicular é um importante método auxiliar no diagnóstico de casos de subfertilidade e infertilidadePrograma de Pós-graduação em PatologiaPatologiaFerreira, Ana Maria Reishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786534Z1Pissinatti, Alcideshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701955Y7Brandão, Felipe Zandonadihttp://lattes.cnpq.br/5996890400232803Jesus, Vera Lucia Teixeira dehttp://lattes.cnpq.br/6838386218159684Mello, Marcela Freire Vallim dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703048Y6Martins, Daniela de Carvalhohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763376J4Leite, Dala Kezen Vieira Hardman2021-03-10T20:45:24Z2010-02-082021-03-10T20:45:24Z2009-07-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18723porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2023-03-01T19:38:58Zoai:app.uff.br:1/18723Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T10:49:26.238728Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
title |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
spellingShingle |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora Leite, Dala Kezen Vieira Hardman Felino doméstico Selvagem Sêmen Eletroejaculação Histopatologia Citologia Testículo Domestic feline Salvage Semen Electroejaculation Histopathology Cytology Testis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
title_full |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
title_fullStr |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
title_full_unstemmed |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
title_sort |
Avaliações das características histológicas, citológicas, clínicas e seminais de felinos domésticos (Felis catus, Linnaeus, 1758) e selvagens (Leopardus tigrinus, Schreber, 1775), Leopardus geoffroyi, D Orbign & Gervais, 1843 e Puma yagouaroundi, E. Geoffroyi, 1803). Felidae Carnívora |
author |
Leite, Dala Kezen Vieira Hardman |
author_facet |
Leite, Dala Kezen Vieira Hardman |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ferreira, Ana Maria Reis http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786534Z1 Pissinatti, Alcides http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701955Y7 Brandão, Felipe Zandonadi http://lattes.cnpq.br/5996890400232803 Jesus, Vera Lucia Teixeira de http://lattes.cnpq.br/6838386218159684 Mello, Marcela Freire Vallim de http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703048Y6 Martins, Daniela de Carvalho http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763376J4 |
dc.contributor.author.fl_str_mv |
Leite, Dala Kezen Vieira Hardman |
dc.subject.por.fl_str_mv |
Felino doméstico Selvagem Sêmen Eletroejaculação Histopatologia Citologia Testículo Domestic feline Salvage Semen Electroejaculation Histopathology Cytology Testis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Felino doméstico Selvagem Sêmen Eletroejaculação Histopatologia Citologia Testículo Domestic feline Salvage Semen Electroejaculation Histopathology Cytology Testis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
The general objective of this work was to evaluate the fertility of male domestic and wild felids through andrological examination and testicular cytology using nonaspiration fine needle cytology. Twenty-five domestic cats (Felis catus, Linnaeus,1758), of various ages, presented for clinical evaluation at the University Veterinary Clinic were examined. Seven adult small wild felids were also examined: five oncillas (Leopardus tigrinus, Schreber, 1775), one Geoffroy s cat (Leopardus geoffroyi, d Orbign & Gervais, 1843), and one jaguarundi (Puma yagouaroundi, E. Geoffroyi, 1803), all belonging to the Fundação RIOZOO (RJ). All animals were submitted to a complete andrological examination and testicular cytology. The animals were kept under anesthesia throughout the whole procedure with the use of ketamine (5 mg/kg), xylazine (0.5 mg/kg) and atropine (0.04 mg/kg) as well as epidural anesthesia with 2% lidocaine without vasoconstrictor (6 mg/kg). Semen samples were collected using the electroejaculation method and examined regarding its macroscopic characteristics (volume, color and odor) as well as microscopic characteristics (motility, vigor and morphology). Following the examination, a testicular cytology using nonaspiration fine needle cytology was performed on both testicles. Testicular cytology revealed cells from the spermatic lineage as well as Sertoli cells. The color of the ejaculate varied from transparent to milky white, while odor showed no alterations and seminal pH varied between 6.0 and 7.0. Mean sperm motility was 60% in domestic cats and 80% in wild felids. Vigor varied between 0 and 5. The average and standard deviation primary defects observed were 22,57 ± 13,44% for domestic cats and 14,75 ± 4,13% for wild felids. The domestic cats secondary defects were 16,20 ± 6,82% and the wild 17,00 ± 6,27%.Mean total sperm defects observed were 38.78 ± 17,11% for domestic cats and 31.75 ± 8,50% for wild felids. Regarding the macroscopic evaluation of the testicles of domestic cats, 70% of the animals had oval-shaped testicles and in the microscopic evaluation, focal hemorrhaging was observed in 88% of cats and 10% of the seminiferous tubules were found to be underdeveloped. The Mann-Whitney test demonstrated that there was no significant difference (p>0.05) between the studied groups. It was concluded that 60% of domestic cats were fertile; within the wild felids, the jaguarondi was the only cat to present compromised reproductive capability; the anesthetic protocol used in this study was found to be efficient for andrological evaluation; there is no correlation between mensurated scrotal perimeter and seminal characteristics; the electroejaculation technique in felids allows for the collection of a good quality semen sample for evaluation; the clinical examination and seminal analysis were essential for fertility evaluation of the male individuals. Testicular cytology using nonaspiration fine needle cytology proved to be a fast, simple and efficient technique; testicular cytology is an important complementary method for the diagnosis of subfertility and infertility |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-30 2010-02-08 2021-03-10T20:45:24Z 2021-03-10T20:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/18723 |
url |
https://app.uff.br/riuff/handle/1/18723 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
_version_ |
1811823580209479680 |