Citotoxicidade de cimentos de ionômero de vidro com adição de nanopartículas de prata

• Autor(a) principal: Siqueira, Patrícia Correia de
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/4429
• topic: Cimentos de ionômeros de vidro; Citotoxicidade; Técnicas de cultura de células; Nanotecnologia; Nanopartículas metálicas; Glass ionomer cements; Cytotoxicity; Cell culture techniques; Nanotechnology; Metal nanoparticles; CIENCIAS DA SAUDE::ODONTOLOGIA

The aim of this study was to evaluate and compare the cytotoxicity of two glass ionomer cements (GIC), a conventional (GC Gold Label 1 – GC Corporation) and a resin modified (Vitrebond – 3M ESPE), both indicated for lining, with and without addition of silver nanoparticles (NAg). The NAg were incorporated at the materials in two different concentrations by weight: 0.1% and 0.2%. Specimens with standardized dimensions (4 x 2 mm) were prepared, and for preparing liquid extracts of the cements, the specimens were immersed in 400 μL of culture medium and incubated at 37°C and 5% CO2 for 48 hours. The extracts obtained were incubated in contact with cells for 48 hours. As negative and positive controls were used respectively culture medium and solution of NAg at 0.78% by weight. To evaluate cellular viability, MTT and Trypan Blue assays were used. Data were subjected to statistical analysis with ANOVA and Tukey (α=0.05). Significant decrease in cell viability was observed in all groups of Vitrebond (p˂0.001) compared to negative control. There were no statistically significant differences between the groups of this cement with and without NAg (p˃0.05). For GC Gold Label 1, no statistically significant differences were observed in cell viability between any of the groups compared with the negative control (p>0.05). There was also no difference between the groups with and without NAg (p>0.05). The positive control showed significant reduction in cell viability (p˂0.001). It is concluded that the NAg did not influence on cytotoxicity of GICs evaluated.