Caracterização Genotípica do HIV-1 em Pacientes dos Estados do Tocantins e Mato Grosso

• Autor(a) principal: VIEGAS, ângela Alves
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/1833
• topic: HMA; HIV-1; Tocantins; Mato Grosso; 1. HIV-1(vírus) Diversidade genotípica 2. Infecção por HIV-1 Pacientes Tocantins (TO) 3. Infecção por HIV-1 Pacientes Mato Grosso (MT); HMA; HIV-1; Tocantins; Mato Grosso; CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA

Introduction: There are limited HIV-1 genetic diversity studies performed in Central West and North Brazil subjects. Objectives: To characterize the HIV-1 genetic diversity in the gag region of patients from the States of Tocantins (TO) and Mato Grosso (MT). Material and Methods: 130 patients infected with HIV-1 were recruited from 2003 to 2006, at the regional Public Health Central Laboratories (LACEN) from TO (n=70) e MT (n=60). The HIV-1 genetic diversity in the gag region was investigated by heteroduplex mobility analysis HMA (HMA gag kits/AIDS Reagent Program/ NIH/ USA). For the gag region the Nested-PCR was performed employing the primers H1G777/ H1P02 and H1gag1584/ g17. HIV-1 subtypes references were amplified using the primers of Nested-PCR second round. HMA gag consists in the migration analysis of the hybrid formed by HIV-1 subtypes references and patient samples after polyacrylamide gel electrophoresis (5%). Results: Males were 60% of the study population in TO and 48,3% in MT. The median age was 34 years (21 l71 years) in TO and 35.5 years (4 64 years) in MT. The risk exposure categories of this study group were: sexual exposure (TO: 72.9%, MT: 93.3%), parenteral risk (TO: 4.3%, MT: 3.4%) and vertical case (MT: 1.4%). The HIV-1 subtypes identified by HMA gag were: B (TO: 82.8%, MT: 50.0%), F (TO: 7.8%, MT: 5.6%), C (TO: 4.7%, MT: 11.1%), B/D (TO: 4.7%, MT: 31.5%) and B/F (MT: 1.8%). Subtype B was predominant among men, women and all risk exposure categories. Conclusions: The genetic diversity analysis of HIV-1 isolates from TO and MT pointed subtype B as the prevalent. The most prevalent second form in TO was subtype F and in MT was subtype C. These results contribute to the genetic molecular mapping of the HIV-1 gag region in the Central West and North Brazil.