Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/3373 |
Resumo: | Peripheral nerves are constant targets of traumatic injuries such as crushing and partial or complete sections resulting in decrease or loss of motor function and sensitivity of the innervated area, its severity will depend on the involvement of nervous structures. The worst damage frame is that of total nerve collapse (neurotmesis), which invariably requires surgical treatment. This study aimed to evaluate the morphological and functional effects of rat’s sciatic nerve regeneration after surgical section tubing with silicone chamber and treatment with low-intensity laser (830 nm) in 126 days. We used two groups, control (GC) and laser (GL) with six animals in each. All animals underwent left sciatic nerve neurotmesis followed by tubing with silicone chamber with five mm distance between nerve stumps. Animals in GL received radiation with a wavelength of 830 nm, energy density of 4.13 J/cm², on eight points from the spinal cord to nerve injury site, totaling 40 days of irradiation divided into two distinct periods. The first stage began at the immediate post-operative period where 20 laser applications were performed on alternate days. The second stage occurred 40 days before performing euthanasia in animals submitted to more than 20 days of laser applications on alternate days. During the experiment, we performed functional assessment through the Sciatic Functional Index and Ankle Angle. After 126 days, the animals were euthanized and the fragments of the nerves were removed and fixed in 10% formalin buffered for histological analysis with HE, Luxol Fast Blue and Picrosirius Red staining, and immunohistochemistry with neurofilament antibody (NF), FGF-2 and S-100. Microscopically, we observed the presence of axonal growth, Wallerian degeneration, inflammatory infiltrate and fascicular reorganization. For HE analysis, we stablished descriptive scores and for the other histological data we carried out quantitative analysis through program Image J. In GL we observed better axonal growth, reorganization of issues, absence of inflammatory infiltrate, greater amount of myelin and less collagen, increased expression of S-100 and NF and lower expression of FGF-2. Regarding the function of the sciatic nerve, no functional improvement was observed. |
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Brito, Luiz Augusto Batistahttp://lattes.cnpq.br/3212353728321020Damasceno, Adilson DonizetiPaulo, Neusa MargaridaBrito, Luiz Augusto BatistaAndraus, Rodrigo Antônio CarvalhoSilva, Marcelo Seixo de Brito eVieira, Marcus FragaSantin, Ana Paula Iglesiashttp://lattes.cnpq.br/7616613572733630Mendonça, Giselle Bonifácio Neves2014-10-17T20:45:01Z2013-12-19MENDONÇA, Giselle Bonifácio Neves. Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone. 2013. 115 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.http://repositorio.bc.ufg.br/tede/handle/tede/3373Peripheral nerves are constant targets of traumatic injuries such as crushing and partial or complete sections resulting in decrease or loss of motor function and sensitivity of the innervated area, its severity will depend on the involvement of nervous structures. The worst damage frame is that of total nerve collapse (neurotmesis), which invariably requires surgical treatment. This study aimed to evaluate the morphological and functional effects of rat’s sciatic nerve regeneration after surgical section tubing with silicone chamber and treatment with low-intensity laser (830 nm) in 126 days. We used two groups, control (GC) and laser (GL) with six animals in each. All animals underwent left sciatic nerve neurotmesis followed by tubing with silicone chamber with five mm distance between nerve stumps. Animals in GL received radiation with a wavelength of 830 nm, energy density of 4.13 J/cm², on eight points from the spinal cord to nerve injury site, totaling 40 days of irradiation divided into two distinct periods. The first stage began at the immediate post-operative period where 20 laser applications were performed on alternate days. The second stage occurred 40 days before performing euthanasia in animals submitted to more than 20 days of laser applications on alternate days. During the experiment, we performed functional assessment through the Sciatic Functional Index and Ankle Angle. After 126 days, the animals were euthanized and the fragments of the nerves were removed and fixed in 10% formalin buffered for histological analysis with HE, Luxol Fast Blue and Picrosirius Red staining, and immunohistochemistry with neurofilament antibody (NF), FGF-2 and S-100. Microscopically, we observed the presence of axonal growth, Wallerian degeneration, inflammatory infiltrate and fascicular reorganization. For HE analysis, we stablished descriptive scores and for the other histological data we carried out quantitative analysis through program Image J. In GL we observed better axonal growth, reorganization of issues, absence of inflammatory infiltrate, greater amount of myelin and less collagen, increased expression of S-100 and NF and lower expression of FGF-2. Regarding the function of the sciatic nerve, no functional improvement was observed.Os nervos periféricos são alvos constantes de lesões de origem traumática, como esmagamento e secções parciais ou totais que resulta em diminuição ou perda da motricidade e da sensibilidade no território inervado, cuja severidade dependerá do acometimento de estruturas nervosas. O pior quadro de lesão é aquele em que ocorre ruptura total do nervo (neurotmese), o qual requer invariavelmente tratamento cirúrgico. Este estudo teve o objetivo de avaliar os efeitos morfológicos e funcionais da regeneração do nervo isquiático de rato após secção cirúrgica, tubulização com câmara de silicone e tratamento com laser de baixa intensidade (830 nm) em 126 dias. Utilizaram-se dois grupos, controle (GC) e laser (GL) com seis animais em cada. Todos os animais foram submetidos à neurotmese do nervo isquiático esquerdo, seguido de tubulização com câmara de silicone com cinco mm de distância entre os cotos nervosos. Os animais do GL receberam radiação com comprimento de onda de 830nm, densidade de energia de 4,13J/cm², em oito pontos, desde a região da medula espinhal até o local de lesão do nervo, totalizando 40 dias de irradiação divididos em dois momentos distintos. O primeiro momento iniciou-se no pós-cirúrgico imediato em que foram realizadas 20 aplicações de laser, em dias alternados. O segundo momento ocorreu 40 dias antes de realizar a eutanásia dos animais com mais 20 dias de aplicações de laser, em dias alternados. Durante o experimento procedeu-se avaliação funcional por meio do Índice Funcional do Ciático e Ângulo do Tarso. Após 126 dias os animais foram submetidos à eutanásia e os fragmentos dos nervos foram retirados e fixados em formol tamponado a 10% para análise histológica, com as colorações de HE, Luxol Fast Blue e Picrosirius Red, e imunoistoquímica com os anticorpos neurofilamento (NF), S100 e FGF-2. Microscopicamente, avaliou-se a presença de proliferação axonal, degeneração Walleriana, reorganização fascicular e infiltrado inflamatório. Para a análise do HE estabeleceram-se escores descritivos e para os demais dados histológicos procedeu-se análise quantitativa por meio do programa Image J. Observou-se no GL melhor proliferação axonal, reorganização dos fascículos, ausência de infiltrado inflamatório, maior quantidade de mielina e menor quantidade de colágeno, maior expressão de S-100 e NF e menor expressão de FGF-2. Com relação à função do nervo isquiático, não se observou melhora funcional.Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-10-17T20:41:06Z No. of bitstreams: 2 Tese - Giselle Bonifacio Neves Mendonça - 2013.pdf: 4442248 bytes, checksum: fd3c1ae469f5bfd89f5767680dc30981 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-10-17T20:45:01Z (GMT) No. of bitstreams: 2 Tese - Giselle Bonifacio Neves Mendonça - 2013.pdf: 4442248 bytes, checksum: fd3c1ae469f5bfd89f5767680dc30981 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-10-17T20:45:01Z (GMT). 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dc.title.por.fl_str_mv |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
dc.title.alternative.eng.fl_str_mv |
Low-level laser therapy (830 nm) on sciatic nerve regeneration of rats submitted to neurotmesis and tubing with silicone chamber |
title |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
spellingShingle |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone Mendonça, Giselle Bonifácio Neves Ângulo do tarso Biomecânica Células de Schwann Fototerapia Imunoistoquímica Ankle angle Biomechanics Immunohistochemical Phototherapy Schwann cells CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
title_short |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
title_full |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
title_fullStr |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
title_full_unstemmed |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
title_sort |
Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone |
author |
Mendonça, Giselle Bonifácio Neves |
author_facet |
Mendonça, Giselle Bonifácio Neves |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Brito, Luiz Augusto Batista |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3212353728321020 |
dc.contributor.advisor-co1.fl_str_mv |
Damasceno, Adilson Donizeti |
dc.contributor.advisor-co2.fl_str_mv |
Paulo, Neusa Margarida |
dc.contributor.referee1.fl_str_mv |
Brito, Luiz Augusto Batista |
dc.contributor.referee2.fl_str_mv |
Andraus, Rodrigo Antônio Carvalho |
dc.contributor.referee3.fl_str_mv |
Silva, Marcelo Seixo de Brito e |
dc.contributor.referee4.fl_str_mv |
Vieira, Marcus Fraga |
dc.contributor.referee5.fl_str_mv |
Santin, Ana Paula Iglesias |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7616613572733630 |
dc.contributor.author.fl_str_mv |
Mendonça, Giselle Bonifácio Neves |
contributor_str_mv |
Brito, Luiz Augusto Batista Damasceno, Adilson Donizeti Paulo, Neusa Margarida Brito, Luiz Augusto Batista Andraus, Rodrigo Antônio Carvalho Silva, Marcelo Seixo de Brito e Vieira, Marcus Fraga Santin, Ana Paula Iglesias |
dc.subject.por.fl_str_mv |
Ângulo do tarso Biomecânica Células de Schwann Fototerapia Imunoistoquímica |
topic |
Ângulo do tarso Biomecânica Células de Schwann Fototerapia Imunoistoquímica Ankle angle Biomechanics Immunohistochemical Phototherapy Schwann cells CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
dc.subject.eng.fl_str_mv |
Ankle angle Biomechanics Immunohistochemical Phototherapy Schwann cells |
dc.subject.cnpq.fl_str_mv |
CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL |
description |
Peripheral nerves are constant targets of traumatic injuries such as crushing and partial or complete sections resulting in decrease or loss of motor function and sensitivity of the innervated area, its severity will depend on the involvement of nervous structures. The worst damage frame is that of total nerve collapse (neurotmesis), which invariably requires surgical treatment. This study aimed to evaluate the morphological and functional effects of rat’s sciatic nerve regeneration after surgical section tubing with silicone chamber and treatment with low-intensity laser (830 nm) in 126 days. We used two groups, control (GC) and laser (GL) with six animals in each. All animals underwent left sciatic nerve neurotmesis followed by tubing with silicone chamber with five mm distance between nerve stumps. Animals in GL received radiation with a wavelength of 830 nm, energy density of 4.13 J/cm², on eight points from the spinal cord to nerve injury site, totaling 40 days of irradiation divided into two distinct periods. The first stage began at the immediate post-operative period where 20 laser applications were performed on alternate days. The second stage occurred 40 days before performing euthanasia in animals submitted to more than 20 days of laser applications on alternate days. During the experiment, we performed functional assessment through the Sciatic Functional Index and Ankle Angle. After 126 days, the animals were euthanized and the fragments of the nerves were removed and fixed in 10% formalin buffered for histological analysis with HE, Luxol Fast Blue and Picrosirius Red staining, and immunohistochemistry with neurofilament antibody (NF), FGF-2 and S-100. Microscopically, we observed the presence of axonal growth, Wallerian degeneration, inflammatory infiltrate and fascicular reorganization. For HE analysis, we stablished descriptive scores and for the other histological data we carried out quantitative analysis through program Image J. In GL we observed better axonal growth, reorganization of issues, absence of inflammatory infiltrate, greater amount of myelin and less collagen, increased expression of S-100 and NF and lower expression of FGF-2. Regarding the function of the sciatic nerve, no functional improvement was observed. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-19 |
dc.date.accessioned.fl_str_mv |
2014-10-17T20:45:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MENDONÇA, Giselle Bonifácio Neves. Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone. 2013. 115 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/3373 |
identifier_str_mv |
MENDONÇA, Giselle Bonifácio Neves. Laser de baixa intensidade na regeneração de nervo isquiático de ratos após tubulização com câmara de silicone. 2013. 115 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/3373 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4581960685150189167 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
5710819066431151437 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 -2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Ciência Animal (EVZ) |
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UFG |
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Brasil |
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Escola de Veterinária e Zootecnia - EVZ (RG) |
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Universidade Federal de Goiás |
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